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Protein

Lactase-like protein

Gene

Lctl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactase-like protein
Alternative name(s):
Klotho/lactase-phlorizin hydrolase-related protein
Gene namesi
Name:Lctl
Synonyms:Klph
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2183549. Lctl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 540520ExtracellularSequence analysisAdd
BLAST
Transmembranei541 – 56121HelicalSequence analysisAdd
BLAST
Topological domaini562 – 5665CytoplasmicSequence analysis

GO - Cellular componenti

  • brush border Source: UniProtKB
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 566546Lactase-like proteinPRO_0000042252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8K1F9.
PRIDEiQ8K1F9.

PTM databases

PhosphoSiteiQ8K1F9.

Expressioni

Tissue specificityi

Kidney and skin.1 Publication

Gene expression databases

BgeeiQ8K1F9.
CleanExiMM_LCTL.
ExpressionAtlasiQ8K1F9. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034969.

Structurei

3D structure databases

ProteinModelPortaliQ8K1F9.
SMRiQ8K1F9. Positions 35-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
GeneTreeiENSGT00550000074452.
HOGENOMiHOG000088630.
HOVERGENiHBG053101.
InParanoidiQ8K1F9.
OMAiFNRWFLD.
OrthoDBiEOG7MH0XV.
PhylomeDBiQ8K1F9.
TreeFamiTF314803.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K1F9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPVWVIILG WILLVPRVGT AWRGPPEEAS FYYGTFPPGF SWGVGSSAYQ
60 70 80 90 100
TEGAWDEDGK GPSIWDAFTH GRKEQVLGGD TADTACDSYY KVQEDIALLK
110 120 130 140 150
ELQVSHYRFS LSWPRLLPTG VRAEQVNKRG IKFYSDFIDA LLKSNITPVV
160 170 180 190 200
TLHHWDLPQM LQVAYGGWQN VSMTRYFSDY ADLCFEVFGD RVKHWLTFSD
210 220 230 240 250
PRTMVEKGYE TGLHAPGLRL QGTGLYVAAH HIIKAHAQAW HSYNNTWRSK
260 270 280 290 300
QHGLVGISLN CDWGEPVDID NPDDIEAAER YLQFCLGWFA NPIYAGDYPQ
310 320 330 340 350
VMKDHIGTKS AEQGLEMSRL PTFSLQEKSY LKGTSDFLGL GHFTTRYITQ
360 370 380 390 400
RKYPSHQGPS YQNDRDLVEL VDPNWPEMGS PWLYSVPWGF RRLLNFAQTQ
410 420 430 440 450
YGDPPIYVTE SGAPQKLHCT QFCDEWRIQY LKGYINEMLK AIKDGVDIKG
460 470 480 490 500
YTSWSLLDKF EWEKGYADKY GFYYVEFNVR NKPRYPKASV QYYKEIITAS
510 520 530 540 550
GFPNPQEVES WRLKALETCS INNQMLATEP LLRHMHVASE IVVPTVCALS
560
ILTAALMLTL LLRRRG
Length:566
Mass (Da):64,826
Last modified:October 1, 2002 - v1
Checksum:iEC50A5730275DD84
GO
Isoform 3 (identifier: Q8K1F9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     529-566: EPLLRHMHVA...MLTLLLRRRG → GDVAETGSPL...SSRDVYFSGS

Note: No experimental confirmation available.
Show »
Length:407
Mass (Da):46,955
Checksum:iDD10330AC0BA30E7
GO

Sequence cautioni

The sequence AAH30631.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 159159Missing in isoform 3. 1 PublicationVSP_015833Add
BLAST
Alternative sequencei529 – 56638EPLLR…LRRRG → GDVAETGSPLHLHLSKHLFR ITLLFCCRSSRDVYFSGS in isoform 3. 1 PublicationVSP_015834Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309072 mRNA. Translation: AAM77699.1.
BC030631 mRNA. Translation: AAH30631.1. Different initiation.
CCDSiCCDS23274.1. [Q8K1F9-1]
RefSeqiNP_665834.1. NM_145835.2. [Q8K1F9-1]
UniGeneiMm.436581.

Genome annotation databases

EnsembliENSMUST00000034969; ENSMUSP00000034969; ENSMUSG00000032401. [Q8K1F9-1]
GeneIDi235435.
KEGGimmu:235435.
UCSCiuc009qbl.1. mouse. [Q8K1F9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309072 mRNA. Translation: AAM77699.1.
BC030631 mRNA. Translation: AAH30631.1. Different initiation.
CCDSiCCDS23274.1. [Q8K1F9-1]
RefSeqiNP_665834.1. NM_145835.2. [Q8K1F9-1]
UniGeneiMm.436581.

3D structure databases

ProteinModelPortaliQ8K1F9.
SMRiQ8K1F9. Positions 35-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034969.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

PTM databases

PhosphoSiteiQ8K1F9.

Proteomic databases

PaxDbiQ8K1F9.
PRIDEiQ8K1F9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034969; ENSMUSP00000034969; ENSMUSG00000032401. [Q8K1F9-1]
GeneIDi235435.
KEGGimmu:235435.
UCSCiuc009qbl.1. mouse. [Q8K1F9-1]

Organism-specific databases

CTDi197021.
MGIiMGI:2183549. Lctl.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
GeneTreeiENSGT00550000074452.
HOGENOMiHOG000088630.
HOVERGENiHBG053101.
InParanoidiQ8K1F9.
OMAiFNRWFLD.
OrthoDBiEOG7MH0XV.
PhylomeDBiQ8K1F9.
TreeFamiTF314803.

Miscellaneous databases

NextBioi382664.
PROiQ8K1F9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1F9.
CleanExiMM_LCTL.
ExpressionAtlasiQ8K1F9. baseline and differential.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel mouse membrane-bound family 1 glycosidase-like protein, which carries an atypical active site structure."
    Ito S., Fujimori T., Hayashizaki Y., Nabeshima Y.
    Biochim. Biophys. Acta 1576:341-345(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: C57BL/6J.
    Tissue: Aorta and Vein.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiLCTL_MOUSE
AccessioniPrimary (citable) accession number: Q8K1F9
Secondary accession number(s): Q8BPR1, Q8K2M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 1, 2002
Last modified: March 16, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although it belongs to the glycosyl hydrolase 1 family, Asp-200 is present instead of the conserved Glu which is an active site residue. It is therefore expected that this protein lacks glycosidase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.