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Protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3

Gene

Alkbh3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (By similarity). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).By similarity1 Publication

Catalytic activityi

N(1)-methyladenosine in mRNA + 2-oxoglutarate + O2 = adenosine in mRNA + formaldehyde + succinate + CO2.By similarity

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Enzyme regulationi

Activated by ascorbate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151SubstrateBy similarity
Metal bindingi191 – 1911Iron; catalyticPROSITE-ProRule annotation
Metal bindingi193 – 1931Iron; catalyticPROSITE-ProRule annotation
Binding sitei194 – 1941SubstrateBy similarity
Metal bindingi257 – 2571Iron; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Curated (EC:1.14.11.-By similarity)
Alternative name(s):
Alkylated DNA repair protein alkB homolog 31 Publication
Short name:
mAbh31 Publication
Gene namesi
Name:Alkbh3Imported
Synonyms:Abh31 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916363. Alkbh3.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Predominantly localizes to the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi193 – 1931D → A: Loss of activity. 1 Publication
Mutagenesisi257 – 2571H → A: Reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3PRO_0000239279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei177 – 1771(4R)-5-hydroxyleucine; alternateBy similarity
Modified residuei177 – 1771(4R)-5-oxoleucine; alternateBy similarity

Keywords - PTMi

Hydroxylation, Oxidation

Proteomic databases

EPDiQ8K1E6.
MaxQBiQ8K1E6.
PaxDbiQ8K1E6.
PeptideAtlasiQ8K1E6.
PRIDEiQ8K1E6.

PTM databases

iPTMnetiQ8K1E6.
PhosphoSiteiQ8K1E6.

Expressioni

Tissue specificityi

Detected in testis, kidney, liver and heart.1 Publication

Gene expression databases

BgeeiQ8K1E6.
CleanExiMM_ALKBH3.
ExpressionAtlasiQ8K1E6. baseline and differential.
GenevisibleiQ8K1E6. MM.

Interactioni

Subunit structurei

Interacts with the TRIP4 complex, notably composed of ASCC3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038721.

Structurei

3D structure databases

ProteinModelPortaliQ8K1E6.
SMRiQ8K1E6. Positions 70-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 278107Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1433Substrate bindingBy similarity
Regioni179 – 1813Alpha-ketoglutarate bindingBy similarity
Regioni269 – 2757Alpha-ketoglutarate bindingBy similarity

Sequence similaritiesi

Belongs to the alkB family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IETS. Eukaryota.
COG3145. LUCA.
GeneTreeiENSGT00530000063618.
HOGENOMiHOG000207105.
HOVERGENiHBG056732.
InParanoidiQ8K1E6.
KOiK10860.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR032854. ALKBH3.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PANTHERiPTHR31212. PTHR31212. 1 hit.
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDKRQRARV QGGWATPTKS QSATQPASPA RSRLSQTAGP AWRSKEQQQC
60 70 80 90 100
DRQFVFKEPQ LVVRAAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDLK
110 120 130 140 150
EADWILEQLC KDVPWKQRMG IREDVTYPQP RLTAWYGELP YTYSRITMEP
160 170 180 190 200
NPHWLPVLWT LKSRIEENTS HTFNSLLCNF YRDEKDSVDW HSDDEPSLGS
210 220 230 240 250
CPVIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD HGTLLIMEGA
260 270 280
TQADWQHRVP KEYHSRQPRV NLTFRTVYPD PRGAPR
Length:286
Mass (Da):33,061
Last modified:October 1, 2002 - v1
Checksum:i2480D0D20A9893F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018196 mRNA. Translation: AAH18196.1.
CCDSiCCDS38184.1.
RefSeqiNP_081220.1. NM_026944.1.
XP_006500186.1. XM_006500123.2.
UniGeneiMm.272498.

Genome annotation databases

EnsembliENSMUST00000040005; ENSMUSP00000038721; ENSMUSG00000040174.
GeneIDi69113.
KEGGimmu:69113.
UCSCiuc008lgn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018196 mRNA. Translation: AAH18196.1.
CCDSiCCDS38184.1.
RefSeqiNP_081220.1. NM_026944.1.
XP_006500186.1. XM_006500123.2.
UniGeneiMm.272498.

3D structure databases

ProteinModelPortaliQ8K1E6.
SMRiQ8K1E6. Positions 70-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038721.

PTM databases

iPTMnetiQ8K1E6.
PhosphoSiteiQ8K1E6.

Proteomic databases

EPDiQ8K1E6.
MaxQBiQ8K1E6.
PaxDbiQ8K1E6.
PeptideAtlasiQ8K1E6.
PRIDEiQ8K1E6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040005; ENSMUSP00000038721; ENSMUSG00000040174.
GeneIDi69113.
KEGGimmu:69113.
UCSCiuc008lgn.1. mouse.

Organism-specific databases

CTDi221120.
MGIiMGI:1916363. Alkbh3.

Phylogenomic databases

eggNOGiENOG410IETS. Eukaryota.
COG3145. LUCA.
GeneTreeiENSGT00530000063618.
HOGENOMiHOG000207105.
HOVERGENiHBG056732.
InParanoidiQ8K1E6.
KOiK10860.

Miscellaneous databases

PROiQ8K1E6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1E6.
CleanExiMM_ALKBH3.
ExpressionAtlasiQ8K1E6. baseline and differential.
GenevisibleiQ8K1E6. MM.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR032854. ALKBH3.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PANTHERiPTHR31212. PTHR31212. 1 hit.
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  2. "Repair of methylation damage in DNA and RNA by mammalian AlkB homologues."
    Lee D.-H., Jin S.-G., Cai S., Chen Y., Pfeifer G.P., O'Connor T.R.
    J. Biol. Chem. 280:39448-39459(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-193 AND HIS-257, TISSUE SPECIFICITY.
  3. "Repair deficient mice reveal mABH2 as the primary oxidative demethylase for repairing 1meA and 3meC lesions in DNA."
    Ringvoll J., Nordstrand L.M., Vaagboe C.B., Talstad V., Reite K., Aas P.A., Lauritzen K.H., Liabakk N.B., Bjoerk A., Doughty R.W., Falnes P.O., Krokan H.E., Klungland A.
    EMBO J. 25:2189-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. Cited for: DISRUPTION PHENOTYPE.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Lung and Testis.

Entry informationi

Entry nameiALKB3_MOUSE
AccessioniPrimary (citable) accession number: Q8K1E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 1, 2002
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.