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Protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3

Gene

Alkbh3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (By similarity). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).By similarity1 Publication

Catalytic activityi

N(1)-methyladenosine in mRNA + 2-oxoglutarate + O2 = adenosine in mRNA + formaldehyde + succinate + CO2.By similarity

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Enzyme regulationi

Activated by ascorbate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115SubstrateBy similarity1
Metal bindingi191Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi193Iron; catalyticPROSITE-ProRule annotation1
Binding sitei194SubstrateBy similarity1
Metal bindingi257Iron; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Curated (EC:1.14.11.-By similarity)
Alternative name(s):
Alkylated DNA repair protein alkB homolog 31 Publication
Short name:
mAbh31 Publication
Gene namesi
Name:Alkbh3Imported
Synonyms:Abh31 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916363. Alkbh3.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Predominantly localizes to the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi193D → A: Loss of activity. 1 Publication1
Mutagenesisi257H → A: Reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002392791 – 286Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177(4R)-5-hydroxyleucine; alternateBy similarity1
Modified residuei177(4R)-5-oxoleucine; alternateBy similarity1

Keywords - PTMi

Hydroxylation, Oxidation

Proteomic databases

EPDiQ8K1E6.
MaxQBiQ8K1E6.
PaxDbiQ8K1E6.
PeptideAtlasiQ8K1E6.
PRIDEiQ8K1E6.

PTM databases

iPTMnetiQ8K1E6.
PhosphoSitePlusiQ8K1E6.

Expressioni

Tissue specificityi

Detected in testis, kidney, liver and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000040174.
CleanExiMM_ALKBH3.
ExpressionAtlasiQ8K1E6. baseline and differential.
GenevisibleiQ8K1E6. MM.

Interactioni

Subunit structurei

Interacts with the ASC-1 complex, notably composed of ASCC3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038721.

Structurei

3D structure databases

ProteinModelPortaliQ8K1E6.
SMRiQ8K1E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 278Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 143Substrate bindingBy similarity3
Regioni179 – 181Alpha-ketoglutarate bindingBy similarity3
Regioni269 – 275Alpha-ketoglutarate bindingBy similarity7

Sequence similaritiesi

Belongs to the alkB family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IETS. Eukaryota.
COG3145. LUCA.
GeneTreeiENSGT00530000063618.
HOGENOMiHOG000207105.
HOVERGENiHBG056732.
InParanoidiQ8K1E6.
KOiK10860.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR032854. ALKBH3.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PANTHERiPTHR31212. PTHR31212. 1 hit.
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDKRQRARV QGGWATPTKS QSATQPASPA RSRLSQTAGP AWRSKEQQQC
60 70 80 90 100
DRQFVFKEPQ LVVRAAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDLK
110 120 130 140 150
EADWILEQLC KDVPWKQRMG IREDVTYPQP RLTAWYGELP YTYSRITMEP
160 170 180 190 200
NPHWLPVLWT LKSRIEENTS HTFNSLLCNF YRDEKDSVDW HSDDEPSLGS
210 220 230 240 250
CPVIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD HGTLLIMEGA
260 270 280
TQADWQHRVP KEYHSRQPRV NLTFRTVYPD PRGAPR
Length:286
Mass (Da):33,061
Last modified:October 1, 2002 - v1
Checksum:i2480D0D20A9893F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018196 mRNA. Translation: AAH18196.1.
CCDSiCCDS38184.1.
RefSeqiNP_081220.1. NM_026944.1.
XP_006500186.1. XM_006500123.3.
UniGeneiMm.272498.

Genome annotation databases

EnsembliENSMUST00000040005; ENSMUSP00000038721; ENSMUSG00000040174.
GeneIDi69113.
KEGGimmu:69113.
UCSCiuc008lgn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018196 mRNA. Translation: AAH18196.1.
CCDSiCCDS38184.1.
RefSeqiNP_081220.1. NM_026944.1.
XP_006500186.1. XM_006500123.3.
UniGeneiMm.272498.

3D structure databases

ProteinModelPortaliQ8K1E6.
SMRiQ8K1E6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038721.

PTM databases

iPTMnetiQ8K1E6.
PhosphoSitePlusiQ8K1E6.

Proteomic databases

EPDiQ8K1E6.
MaxQBiQ8K1E6.
PaxDbiQ8K1E6.
PeptideAtlasiQ8K1E6.
PRIDEiQ8K1E6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040005; ENSMUSP00000038721; ENSMUSG00000040174.
GeneIDi69113.
KEGGimmu:69113.
UCSCiuc008lgn.1. mouse.

Organism-specific databases

CTDi221120.
MGIiMGI:1916363. Alkbh3.

Phylogenomic databases

eggNOGiENOG410IETS. Eukaryota.
COG3145. LUCA.
GeneTreeiENSGT00530000063618.
HOGENOMiHOG000207105.
HOVERGENiHBG056732.
InParanoidiQ8K1E6.
KOiK10860.

Miscellaneous databases

PROiQ8K1E6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040174.
CleanExiMM_ALKBH3.
ExpressionAtlasiQ8K1E6. baseline and differential.
GenevisibleiQ8K1E6. MM.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR032854. ALKBH3.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PANTHERiPTHR31212. PTHR31212. 1 hit.
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALKB3_MOUSE
AccessioniPrimary (citable) accession number: Q8K1E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.