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Q8K1E0 (STX5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-5
Gene names
Name:Stx5
Synonyms:Stx5a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates endoplasmic reticulum to Golgi transport By similarity.

Subunit structure

Part of a ternary complex containing STX5A, NSFL1C and VCP. Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR1, GOSR2, YKT6 and VTI1A. Interacts with COG4 By similarity. Ref.4

Subcellular location

Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein By similarity. Golgi apparatus membrane By similarity. Note: Localizes throughout the Golgi apparatus, but most abundant in the cis-most cisternae By similarity. Ref.4

Sequence similarities

Belongs to the syntaxin family.

Contains 1 t-SNARE coiled-coil homology domain.

Caution

It is uncertain whether Met-1 or Met-55 is the initiator. However, for the rat ortholog it has been shown that the equivalent alternative isoform isproduced by alternative initiation at a conserved initiator methionine at position 55.

Sequence caution

The sequence AAH04849.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAH21883.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q8K1E0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q8K1E0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.
Note: Produced by alternative initiation at Met-55 of isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Syntaxin-5
PRO_0000210206

Regions

Topological domain1 – 333333Cytoplasmic Potential
Transmembrane334 – 35421Helical; Anchor for type IV membrane protein; Potential
Topological domain3551Vesicular Potential
Domain263 – 32563t-SNARE coiled-coil homology
Coiled coil287 – 31832 Potential

Natural variations

Alternative sequence1 – 5454Missing in isoform 2.
VSP_020118

Experimental info

Sequence conflict911C → S in AAH21883. Ref.3
Sequence conflict179 – 1802LQ → FE in AAF36981. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 3.
Checksum: 29CFD4EA5665CFEF

FASTA35539,713
        10         20         30         40         50         60 
MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AISSSSDSTP LPTPVALVPS PPDTMSCRDR 

        70         80         90        100        110        120 
TQEFQSACKS LQSRQNGIQT SKPALHAARQ CSEFTLMARR IGKDLSNTFA KLEKLTILAK 

       130        140        150        160        170        180 
RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ 

       190        200        210        220        230        240 
SKLASMSNDF KSVLEVRTEN LKQQRNRREQ FSRAPVSALP LAPNNLGGGP IILGAESRAS 

       250        260        270        280        290        300 
RDVAIDMMDP RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET 

       310        320        330        340        350 
IQRIDENVLG AQLDVEAAHS EILKYFQSVT SNRWLMVKIF LILIVFFIIF VVFLA 

« Hide

Isoform 2 [UniParc].

Checksum: 561706F552EB9D93
Show »

FASTA30134,103

References

« Hide 'large scale' references
[1]"Mouse syntaxin 5."
Low D.Y.H., Tang B.L., Hong W.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N-3.
Tissue: Mammary gland.
[4]"A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway."
Xu Y., Wong S.H., Tang B.L., Subramaniam V.N., Zhang T., Hong W.
J. Biol. Chem. 273:21783-21789(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN SNARE COMPLEX WITH VTI1A, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF232709 mRNA. Translation: AAF36981.1.
AK166087 mRNA. Translation: BAE38563.1.
BC004849 mRNA. Translation: AAH04849.1. Different initiation.
BC021883 mRNA. Translation: AAH21883.1. Different initiation.
CCDSCCDS29542.2. [Q8K1E0-1]
RefSeqNP_001161271.1. NM_001167799.1. [Q8K1E0-1]
NP_062803.4. NM_019829.4. [Q8K1E0-1]
XP_006527287.1. XM_006527224.1. [Q8K1E0-1]
XP_006527288.1. XM_006527225.1. [Q8K1E0-1]
XP_006527289.1. XM_006527226.1. [Q8K1E0-1]
UniGeneMm.153061.

3D structure databases

ProteinModelPortalQ8K1E0.
SMRQ8K1E0. Positions 263-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207947. 1 interaction.
IntActQ8K1E0. 1 interaction.
MINTMINT-4136014.

PTM databases

PhosphoSiteQ8K1E0.

Proteomic databases

MaxQBQ8K1E0.
PaxDbQ8K1E0.
PRIDEQ8K1E0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000073430; ENSMUSP00000073136; ENSMUSG00000010110. [Q8K1E0-1]
ENSMUST00000176381; ENSMUSP00000134854; ENSMUSG00000010110. [Q8K1E0-1]
GeneID56389.
KEGGmmu:56389.
UCSCuc008gmo.2. mouse. [Q8K1E0-1]

Organism-specific databases

CTD56389.
MGIMGI:1928483. Stx5a.

Phylogenomic databases

eggNOGNOG326964.
GeneTreeENSGT00390000000652.
HOGENOMHOG000194860.
HOVERGENHBG001973.
InParanoidQ8K1E0.
KOK08490.
PhylomeDBQ8K1E0.
TreeFamTF315068.

Gene expression databases

ArrayExpressQ8K1E0.
BgeeQ8K1E0.
CleanExMM_STX5A.
GenevestigatorQ8K1E0.

Family and domain databases

InterProIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMSSF47661. SSF47661. 1 hit.
PROSITEPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio312484.
PROQ8K1E0.
SOURCESearch...

Entry information

Entry nameSTX5_MOUSE
AccessionPrimary (citable) accession number: Q8K1E0
Secondary accession number(s): Q3TM79, Q99J28, Q9JKP2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot