Q8K1B9 (GLTL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative polypeptide N-acetylgalactosaminyltransferase-like protein 4
EC=2.4.1.41

Alternative name(s):
Polypeptide GalNAc transferase-like protein 4
Short name=GalNAc-T-like protein 4
Short name=pp-GaNTase-like protein 4
Protein-UDP acetylgalactosaminyltransferase-like protein 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4

Gene names

Name:

Galntl4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide.
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polypeptide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Putative polypeptide N-acetylgalactosaminyltransferase-like protein 4		PRO_0000059142
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 35	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 622	587	Lumenal Potential
	Domain	484 – 614	131	Ricin B-type lectin
	Region	153 – 267	115	Catalytic subdomain A
	Region	324 – 400	77	Catalytic subdomain B
Amino acid modifications
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
	Disulfide bond	497 ↔ 513		By similarity
	Disulfide bond	545 ↔ 558		By similarity
	Disulfide bond	586 ↔ 606		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K1B9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 4FE9493795E3A561	FASTA	622	71,096
	10 20 30 40 50 60 MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPVPDK KLEEDKGDTL 70 80 90 100 110 120 KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH WGQELSPEGR RVALKQFQYY 130 140 150 160 170 180 GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP DSLPEVSIVF IFVNEALSVL LRSIHSAMER 190 200 210 220 230 240 TPSHLLKEII LVDDNSSNEE LKEKLTEYVD KVNGQKPGFI KVVRHSKQEG LIRSRVSGWR 250 260 270 280 290 300 AATAPVVALF DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF 310 320 330 340 350 360 DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FEEIGLLDEG MEVYGGENVE 370 380 390 400 410 420 LGIRVSEISH TGLSSAPMMV WQCGGSVEVL PCSRIAHIER AHKPYTEDLT AHVRRNALRV 430 440 450 460 470 480 AEVWMDEFKS HVYMAWNIPQ EDSGIDIGDI TARKALRKQL QCKTFRWYLV SVYPEMRMYS 490 500 510 520 530 540 DIIAYGVLQN SLKTDLCLDQ GPDTENVPIV YICHGMTPQN VYYTSSQQIH VGILSPTVDD 550 560 570 580 590 600 DDNRCLVDVN SRPRLIECSY AKAKRMKLHW QFSQGGSIQN RKSKRCLELQ ENSDMEFGFQ 610 620 LVLQKCSGQH WTITNVLRSL VS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Putative polypeptide N-acetylgalactosaminyltransferase-like protein 4

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC024988 mRNA. Translation: AAH24988.1.
IPI	IPI00230295.
RefSeq	NP_776100.2. NM_173739.3.
UniGene	Mm.323322.
3D structure databases
ProteinModelPortal	Q8K1B9.
SMR	Q8K1B9. Positions 102-615.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K1B9.
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27.
Proteomic databases
PRIDE	Q8K1B9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049430; ENSMUSP00000043636; ENSMUSG00000038296.
GeneID	233733.
KEGG	mmu:233733.
UCSC	uc009jgc.1. mouse.
Organism-specific databases
CTD	374378.
MGI	MGI:2446239. Galntl4.
Phylogenomic databases
eggNOG	roNOG08578.
GeneTree	ENSGT00560000076662.
HOGENOM	HBG715048.
HOVERGEN	HBG051699.
InParanoid	Q8K1B9.
OMA	PIQNRKS.
OrthoDB	EOG4M91R2.
PhylomeDB	Q8K1B9.
Gene expression databases
ArrayExpress	Q8K1B9.
Bgee	Q8K1B9.
Genevestigator	Q8K1B9.
GermOnline	ENSMUSG00000038296. Mus musculus.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR008997. Ricin_B-rel_lectin. IPR000772. Ricin_B_lectin. [Graphical view]
KO	K00710.
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
SUPFAM	SSF50370. RicinB_like. 1 hit.
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	381803.
SOURCE	Search...

Entry information

Entry name

GLTL4_MOUSE

Accession

Primary (citable) accession number: Q8K1B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2002
Last modified:	November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program