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Q8K1B9

- GLT18_MOUSE

UniProt

Q8K1B9 - GLT18_MOUSE

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Protein

Polypeptide N-acetylgalactosaminyltransferase 18

Gene

Galnt18

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei194 – 1941SubstrateBy similarity
Metal bindingi251 – 2511ManganeseBy similarity
Metal bindingi253 – 2531ManganeseBy similarity
Metal bindingi397 – 3971ManganeseBy similarity
Binding sitei400 – 4001SubstrateBy similarity
Binding sitei405 – 4051SubstrateBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 18 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 18
Short name:
GalNAc-T18
Polypeptide GalNAc transferase-like protein 4
Short name:
GalNAc-T-like protein 4
Short name:
pp-GaNTase-like protein 4
Polypeptide N-acetylgalactosaminyltransferase-like protein 4
Protein-UDP acetylgalactosaminyltransferase-like protein 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
Gene namesi
Name:Galnt18
Synonyms:Galntl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2446239. Galnt18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3523Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini36 – 622587LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Polypeptide N-acetylgalactosaminyltransferase 18PRO_0000059142Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi144 ↔ 392PROSITE-ProRule annotation
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi383 ↔ 462PROSITE-ProRule annotation
Disulfide bondi497 ↔ 513PROSITE-ProRule annotation
Disulfide bondi545 ↔ 558PROSITE-ProRule annotation
Disulfide bondi586 ↔ 606PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8K1B9.

PTM databases

PhosphoSiteiQ8K1B9.

Expressioni

Gene expression databases

BgeeiQ8K1B9.
ExpressionAtlasiQ8K1B9. baseline and differential.
GenevestigatoriQ8K1B9.

Structurei

3D structure databases

ProteinModelPortaliQ8K1B9.
SMRiQ8K1B9. Positions 121-616.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini484 – 614131Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 267115Catalytic subdomain AAdd
BLAST
Regioni324 – 40077Catalytic subdomain BAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG123124.
GeneTreeiENSGT00670000097647.
HOGENOMiHOG000038228.
HOVERGENiHBG051699.
InParanoidiQ8K1B9.
KOiK00710.
OMAiAPDKKLE.
OrthoDBiEOG70087G.
PhylomeDBiQ8K1B9.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1B9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPVPDK
60 70 80 90 100
KLEEDKGDTL KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH
110 120 130 140 150
WGQELSPEGR RVALKQFQYY GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP
160 170 180 190 200
DSLPEVSIVF IFVNEALSVL LRSIHSAMER TPSHLLKEII LVDDNSSNEE
210 220 230 240 250
LKEKLTEYVD KVNGQKPGFI KVVRHSKQEG LIRSRVSGWR AATAPVVALF
260 270 280 290 300
DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF
310 320 330 340 350
DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FEEIGLLDEG
360 370 380 390 400
MEVYGGENVE LGIRVSEISH TGLSSAPMMV WQCGGSVEVL PCSRIAHIER
410 420 430 440 450
AHKPYTEDLT AHVRRNALRV AEVWMDEFKS HVYMAWNIPQ EDSGIDIGDI
460 470 480 490 500
TARKALRKQL QCKTFRWYLV SVYPEMRMYS DIIAYGVLQN SLKTDLCLDQ
510 520 530 540 550
GPDTENVPIV YICHGMTPQN VYYTSSQQIH VGILSPTVDD DDNRCLVDVN
560 570 580 590 600
SRPRLIECSY AKAKRMKLHW QFSQGGSIQN RKSKRCLELQ ENSDMEFGFQ
610 620
LVLQKCSGQH WTITNVLRSL VS
Length:622
Mass (Da):71,096
Last modified:October 1, 2002 - v1
Checksum:i4FE9493795E3A561
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024988 mRNA. Translation: AAH24988.1.
CCDSiCCDS21751.1.
RefSeqiNP_776100.2. NM_173739.3.
UniGeneiMm.323322.

Genome annotation databases

EnsembliENSMUST00000049430; ENSMUSP00000043636; ENSMUSG00000038296.
GeneIDi233733.
KEGGimmu:233733.
UCSCiuc009jgc.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Putative polypeptide N-acetylgalactosaminyltransferase-like protein 4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024988 mRNA. Translation: AAH24988.1 .
CCDSi CCDS21751.1.
RefSeqi NP_776100.2. NM_173739.3.
UniGenei Mm.323322.

3D structure databases

ProteinModelPortali Q8K1B9.
SMRi Q8K1B9. Positions 121-616.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSitei Q8K1B9.

Proteomic databases

PRIDEi Q8K1B9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049430 ; ENSMUSP00000043636 ; ENSMUSG00000038296 .
GeneIDi 233733.
KEGGi mmu:233733.
UCSCi uc009jgc.1. mouse.

Organism-specific databases

CTDi 374378.
MGIi MGI:2446239. Galnt18.

Phylogenomic databases

eggNOGi NOG123124.
GeneTreei ENSGT00670000097647.
HOGENOMi HOG000038228.
HOVERGENi HBG051699.
InParanoidi Q8K1B9.
KOi K00710.
OMAi APDKKLE.
OrthoDBi EOG70087G.
PhylomeDBi Q8K1B9.
TreeFami TF313267.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 381803.
PROi Q8K1B9.
SOURCEi Search...

Gene expression databases

Bgeei Q8K1B9.
ExpressionAtlasi Q8K1B9. baseline and differential.
Genevestigatori Q8K1B9.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.

Entry informationi

Entry nameiGLT18_MOUSE
AccessioniPrimary (citable) accession number: Q8K1B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3