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Q8K1B9

- GLT18_MOUSE

UniProt

Q8K1B9 - GLT18_MOUSE

Protein

Polypeptide N-acetylgalactosaminyltransferase 18

Gene

Galnt18

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941SubstrateBy similarity
    Metal bindingi251 – 2511ManganeseBy similarity
    Metal bindingi253 – 2531ManganeseBy similarity
    Metal bindingi397 – 3971ManganeseBy similarity
    Binding sitei400 – 4001SubstrateBy similarity
    Binding sitei405 – 4051SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 18 (EC:2.4.1.41)
    Alternative name(s):
    Polypeptide GalNAc transferase 18
    Short name:
    GalNAc-T18
    Polypeptide GalNAc transferase-like protein 4
    Short name:
    GalNAc-T-like protein 4
    Short name:
    pp-GaNTase-like protein 4
    Polypeptide N-acetylgalactosaminyltransferase-like protein 4
    Protein-UDP acetylgalactosaminyltransferase-like protein 4
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
    Gene namesi
    Name:Galnt18
    Synonyms:Galntl4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2446239. Galnt18.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Polypeptide N-acetylgalactosaminyltransferase 18PRO_0000059142Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi144 ↔ 392PROSITE-ProRule annotation
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi383 ↔ 462PROSITE-ProRule annotation
    Disulfide bondi497 ↔ 513PROSITE-ProRule annotation
    Disulfide bondi545 ↔ 558PROSITE-ProRule annotation
    Disulfide bondi586 ↔ 606PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8K1B9.

    PTM databases

    PhosphoSiteiQ8K1B9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K1B9.
    BgeeiQ8K1B9.
    GenevestigatoriQ8K1B9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1B9.
    SMRiQ8K1B9. Positions 121-616.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 622587LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3523Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini484 – 614131Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni153 – 267115Catalytic subdomain AAdd
    BLAST
    Regioni324 – 40077Catalytic subdomain BAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG123124.
    GeneTreeiENSGT00670000097647.
    HOGENOMiHOG000038228.
    HOVERGENiHBG051699.
    InParanoidiQ8K1B9.
    KOiK00710.
    OMAiAPDKKLE.
    OrthoDBiEOG70087G.
    PhylomeDBiQ8K1B9.
    TreeFamiTF313267.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K1B9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPVPDK    50
    KLEEDKGDTL KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH 100
    WGQELSPEGR RVALKQFQYY GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP 150
    DSLPEVSIVF IFVNEALSVL LRSIHSAMER TPSHLLKEII LVDDNSSNEE 200
    LKEKLTEYVD KVNGQKPGFI KVVRHSKQEG LIRSRVSGWR AATAPVVALF 250
    DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF 300
    DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FEEIGLLDEG 350
    MEVYGGENVE LGIRVSEISH TGLSSAPMMV WQCGGSVEVL PCSRIAHIER 400
    AHKPYTEDLT AHVRRNALRV AEVWMDEFKS HVYMAWNIPQ EDSGIDIGDI 450
    TARKALRKQL QCKTFRWYLV SVYPEMRMYS DIIAYGVLQN SLKTDLCLDQ 500
    GPDTENVPIV YICHGMTPQN VYYTSSQQIH VGILSPTVDD DDNRCLVDVN 550
    SRPRLIECSY AKAKRMKLHW QFSQGGSIQN RKSKRCLELQ ENSDMEFGFQ 600
    LVLQKCSGQH WTITNVLRSL VS 622
    Length:622
    Mass (Da):71,096
    Last modified:October 1, 2002 - v1
    Checksum:i4FE9493795E3A561
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC024988 mRNA. Translation: AAH24988.1.
    CCDSiCCDS21751.1.
    RefSeqiNP_776100.2. NM_173739.3.
    UniGeneiMm.323322.

    Genome annotation databases

    EnsembliENSMUST00000049430; ENSMUSP00000043636; ENSMUSG00000038296.
    GeneIDi233733.
    KEGGimmu:233733.
    UCSCiuc009jgc.1. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Putative polypeptide N-acetylgalactosaminyltransferase-like protein 4

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC024988 mRNA. Translation: AAH24988.1 .
    CCDSi CCDS21751.1.
    RefSeqi NP_776100.2. NM_173739.3.
    UniGenei Mm.323322.

    3D structure databases

    ProteinModelPortali Q8K1B9.
    SMRi Q8K1B9. Positions 121-616.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    PTM databases

    PhosphoSitei Q8K1B9.

    Proteomic databases

    PRIDEi Q8K1B9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049430 ; ENSMUSP00000043636 ; ENSMUSG00000038296 .
    GeneIDi 233733.
    KEGGi mmu:233733.
    UCSCi uc009jgc.1. mouse.

    Organism-specific databases

    CTDi 374378.
    MGIi MGI:2446239. Galnt18.

    Phylogenomic databases

    eggNOGi NOG123124.
    GeneTreei ENSGT00670000097647.
    HOGENOMi HOG000038228.
    HOVERGENi HBG051699.
    InParanoidi Q8K1B9.
    KOi K00710.
    OMAi APDKKLE.
    OrthoDBi EOG70087G.
    PhylomeDBi Q8K1B9.
    TreeFami TF313267.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 381803.
    PROi Q8K1B9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K1B9.
    Bgeei Q8K1B9.
    Genevestigatori Q8K1B9.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.

    Entry informationi

    Entry nameiGLT18_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1B9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3