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Protein

Fermitin family homolog 3

Gene

Fermt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in cell adhesion in hematopoietic cells (By similarity). Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3) (PubMed:18278053). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells (PubMed:19234461). Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs) (PubMed:18278053).By similarity3 Publications

GO - Molecular functioni

  • integrin binding Source: UniProtKB

GO - Biological processi

  • integrin activation Source: UniProtKB
  • integrin-mediated signaling pathway Source: UniProtKB
  • leukocyte cell-cell adhesion Source: UniProtKB
  • platelet aggregation Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • regulation of cell-cell adhesion mediated by integrin Source: UniProtKB
  • substrate adhesion-dependent cell spreading Source: UniProtKB

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Fermitin family homolog 3
Alternative name(s):
Kindlin-3
Unc-112-related protein 2
Gene namesi
Name:Fermt3
Synonyms:Kind3, Urp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2147790. Fermt3.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell projection Source: UniProtKB-KW
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • podosome Source: UniProtKB

Keywords - Cellular componenti

Cell junction, Cell projection

Pathology & Biotechi

Disruption phenotypei

Mice suffer from fatal anemia, is caused by severe bleeding and erythrocytopenia. mice also show markedly reduced size and cellularity. Platelets cannot activate integrins despite normal Talin (Tln1) expression.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi597Q → A: Impairs ability to activsate integrins. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194551 – 665Fermitin family homolog 3Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei11PhosphotyrosineBy similarity1
Modified residuei502PhosphotyrosineBy similarity1
Modified residuei589PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K1B8.
MaxQBiQ8K1B8.
PaxDbiQ8K1B8.
PRIDEiQ8K1B8.

PTM databases

iPTMnetiQ8K1B8.
PhosphoSitePlusiQ8K1B8.

Expressioni

Tissue specificityi

Specifically expressed in hematopoietic cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000024965.
CleanExiMM_FERMT3.
ExpressionAtlasiQ8K1B8. baseline and differential.
GenevisibleiQ8K1B8. MM.

Interactioni

Subunit structurei

Interacts with ITGB1, ITGB2 and ITGB3 (via cytoplasmic tails).2 Publications

GO - Molecular functioni

  • integrin binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8K1B8. 1 interactor.
STRINGi10090.ENSMUSP00000037858.

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi351 – 359Combined sources9
Beta strandi370 – 377Combined sources8
Beta strandi380 – 387Combined sources8
Turni388 – 392Combined sources5
Beta strandi395 – 399Combined sources5
Beta strandi404 – 410Combined sources7
Turni411 – 414Combined sources4
Beta strandi415 – 424Combined sources10
Beta strandi427 – 434Combined sources8
Helixi438 – 452Combined sources15
Helixi462 – 476Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5L81X-ray2.23A/B344-478[»]
ProteinModelPortaliQ8K1B8.
SMRiQ8K1B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini229 – 556FERMAdd BLAST328
Domaini357 – 453PHPROSITE-ProRule annotationAdd BLAST97

Domaini

The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

Sequence similaritiesi

Belongs to the kindlin family.Curated

Phylogenomic databases

eggNOGiKOG3727. Eukaryota.
ENOG410XS1B. LUCA.
GeneTreeiENSGT00390000013444.
HOGENOMiHOG000231715.
HOVERGENiHBG020688.
InParanoidiQ8K1B8.
KOiK17084.
OMAiQMAIEFE.
OrthoDBiEOG091G03SD.
PhylomeDBiQ8K1B8.
TreeFamiTF314677.

Family and domain databases

CDDicd14473. FERM_B-lobe. 1 hit.
Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 2 hits.
InterProiView protein in InterPro
IPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
PfamiView protein in Pfam
PF00373. FERM_M. 1 hit.
PF00169. PH. 1 hit.
SMARTiView protein in SMART
SM00295. B41. 1 hit.
SM00233. PH. 1 hit.
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiView protein in PROSITE
PS00661. FERM_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K1B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGMKTASGD YIDSSWELRV FVGEEDPEAQ SVTLRVTGES HIGGVLLKIV
60 70 80 90 100
EEINRKQDWS DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV
110 120 130 140 150
ILRLPNRRVL RLRASFSKPL FQTVAAICRL LSIRHPEELS LLRAPEKKEK
160 170 180 190 200
KKKEKEPEEE VHDLTKVVLA GGVAPTLFRG MPAHFSDSAQ TEACYHMLSR
210 220 230 240 250
PQPAPDPLLL QRLPRPSSLP DKTQLHSRWL DSSRCLMQQG IKAGDVLWLR
260 270 280 290 300
FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL TEEIDCTEEE MMVFAALQYH
310 320 330 340 350
INKLTLSGDV GELASGDLGL DDLDAALNNL EVKLKGSAPS DMLDSLTTIP
360 370 380 390 400
ELKDHLRIFR PRKLTLKGYR QYWVVFKDTT LSYYKSQDEA PGDPTQQLNL
410 420 430 440 450
KGCEVVPDVN VSGQKFCIKL LVPSPEGMSE IYLRCQDEQQ YAQWMAACRL
460 470 480 490 500
ASKGRTMADS SYASEVQAIL AFLSLQRAGG SNGGSGNKPQ GPEAPAEGLN
510 520 530 540 550
PYGLVAPRFQ RKFKAKQLTP RILEAHQNVA QLSLTEAQLR FIQAWQSLPD
560 570 580 590 600
FGISYVMVRF KGSRKDEILG IANNRLIRID LAVGDVVKTW RFSNMRQWNV
610 620 630 640 650
NWDIRQVAIE FDEHINVAFS CVSASCRIVH EYIGGYIFLS TRERARGEEL
660
DEDLFLQLTG GHEAF
Length:665
Mass (Da):75,635
Last modified:October 1, 2002 - v1
Checksum:iB0BDC2E6FC3D0894
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025119 mRNA. Translation: AAH25119.1.
BC032204 mRNA. Translation: AAH32204.1.
CCDSiCCDS29518.1.
RefSeqiNP_722490.1. NM_153795.2.
XP_006526656.1. XM_006526593.3.
UniGeneiMm.157591.

Genome annotation databases

EnsembliENSMUST00000040772; ENSMUSP00000037858; ENSMUSG00000024965.
GeneIDi108101.
KEGGimmu:108101.
UCSCiuc008gkd.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiURP2_MOUSE
AccessioniPrimary (citable) accession number: Q8K1B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: October 1, 2002
Last modified: June 7, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families