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Protein

Fermitin family homolog 3

Gene

Fermt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in cell adhesion in hematopoietic cells (By similarity). Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3) (PubMed:18278053). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells (PubMed:19234461). Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs) (PubMed:18278053).By similarity3 Publications

GO - Molecular functioni

  • integrin binding Source: UniProtKB

GO - Biological processi

  • integrin activation Source: UniProtKB
  • integrin-mediated signaling pathway Source: UniProtKB
  • leukocyte cell-cell adhesion Source: UniProtKB
  • platelet aggregation Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • regulation of cell-cell adhesion mediated by integrin Source: UniProtKB
  • substrate adhesion-dependent cell spreading Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Fermitin family homolog 3
Alternative name(s):
Kindlin-3
Unc-112-related protein 2
Gene namesi
Name:Fermt3
Synonyms:Kind3, Urp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2147790. Fermt3.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell projection Source: UniProtKB-KW
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • podosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection

Pathology & Biotechi

Disruption phenotypei

Mice suffer from fatal anemia, is caused by severe bleeding and erythrocytopenia. mice also show markedly reduced size and cellularity. Platelets cannot activate integrins despite normal Talin (Tln1) expression.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi597 – 5971Q → A: Impairs ability to activsate integrins. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 665665Fermitin family homolog 3PRO_0000219455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphotyrosineBy similarity
Modified residuei502 – 5021PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K1B8.
MaxQBiQ8K1B8.
PaxDbiQ8K1B8.
PRIDEiQ8K1B8.

PTM databases

iPTMnetiQ8K1B8.
PhosphoSiteiQ8K1B8.

Expressioni

Tissue specificityi

Specifically expressed in hematopoietic cells.1 Publication

Gene expression databases

BgeeiQ8K1B8.
CleanExiMM_FERMT3.
ExpressionAtlasiQ8K1B8. baseline and differential.
GenevisibleiQ8K1B8. MM.

Interactioni

Subunit structurei

Interacts with ITGB1, ITGB2 and ITGB3 (via cytoplasmic tails).2 Publications

GO - Molecular functioni

  • integrin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037858.

Structurei

3D structure databases

ProteinModelPortaliQ8K1B8.
SMRiQ8K1B8. Positions 7-98, 349-477, 521-637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini229 – 556328FERMAdd
BLAST
Domaini357 – 45397PHPROSITE-ProRule annotationAdd
BLAST

Domaini

The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

Sequence similaritiesi

Belongs to the kindlin family.Curated
Contains 1 FERM domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3727. Eukaryota.
ENOG410XS1B. LUCA.
GeneTreeiENSGT00390000013444.
HOGENOMiHOG000231715.
HOVERGENiHBG020688.
InParanoidiQ8K1B8.
KOiK17084.
OMAiEMMLFGA.
OrthoDBiEOG7T7GSC.
PhylomeDBiQ8K1B8.
TreeFamiTF314677.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 2 hits.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00295. B41. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00661. FERM_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGMKTASGD YIDSSWELRV FVGEEDPEAQ SVTLRVTGES HIGGVLLKIV
60 70 80 90 100
EEINRKQDWS DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV
110 120 130 140 150
ILRLPNRRVL RLRASFSKPL FQTVAAICRL LSIRHPEELS LLRAPEKKEK
160 170 180 190 200
KKKEKEPEEE VHDLTKVVLA GGVAPTLFRG MPAHFSDSAQ TEACYHMLSR
210 220 230 240 250
PQPAPDPLLL QRLPRPSSLP DKTQLHSRWL DSSRCLMQQG IKAGDVLWLR
260 270 280 290 300
FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL TEEIDCTEEE MMVFAALQYH
310 320 330 340 350
INKLTLSGDV GELASGDLGL DDLDAALNNL EVKLKGSAPS DMLDSLTTIP
360 370 380 390 400
ELKDHLRIFR PRKLTLKGYR QYWVVFKDTT LSYYKSQDEA PGDPTQQLNL
410 420 430 440 450
KGCEVVPDVN VSGQKFCIKL LVPSPEGMSE IYLRCQDEQQ YAQWMAACRL
460 470 480 490 500
ASKGRTMADS SYASEVQAIL AFLSLQRAGG SNGGSGNKPQ GPEAPAEGLN
510 520 530 540 550
PYGLVAPRFQ RKFKAKQLTP RILEAHQNVA QLSLTEAQLR FIQAWQSLPD
560 570 580 590 600
FGISYVMVRF KGSRKDEILG IANNRLIRID LAVGDVVKTW RFSNMRQWNV
610 620 630 640 650
NWDIRQVAIE FDEHINVAFS CVSASCRIVH EYIGGYIFLS TRERARGEEL
660
DEDLFLQLTG GHEAF
Length:665
Mass (Da):75,635
Last modified:October 1, 2002 - v1
Checksum:iB0BDC2E6FC3D0894
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025119 mRNA. Translation: AAH25119.1.
BC032204 mRNA. Translation: AAH32204.1.
CCDSiCCDS29518.1.
RefSeqiNP_722490.1. NM_153795.2.
XP_006526656.1. XM_006526593.2.
UniGeneiMm.157591.

Genome annotation databases

EnsembliENSMUST00000040772; ENSMUSP00000037858; ENSMUSG00000024965.
GeneIDi108101.
KEGGimmu:108101.
UCSCiuc008gkd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025119 mRNA. Translation: AAH25119.1.
BC032204 mRNA. Translation: AAH32204.1.
CCDSiCCDS29518.1.
RefSeqiNP_722490.1. NM_153795.2.
XP_006526656.1. XM_006526593.2.
UniGeneiMm.157591.

3D structure databases

ProteinModelPortaliQ8K1B8.
SMRiQ8K1B8. Positions 7-98, 349-477, 521-637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037858.

PTM databases

iPTMnetiQ8K1B8.
PhosphoSiteiQ8K1B8.

Proteomic databases

EPDiQ8K1B8.
MaxQBiQ8K1B8.
PaxDbiQ8K1B8.
PRIDEiQ8K1B8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040772; ENSMUSP00000037858; ENSMUSG00000024965.
GeneIDi108101.
KEGGimmu:108101.
UCSCiuc008gkd.2. mouse.

Organism-specific databases

CTDi83706.
MGIiMGI:2147790. Fermt3.

Phylogenomic databases

eggNOGiKOG3727. Eukaryota.
ENOG410XS1B. LUCA.
GeneTreeiENSGT00390000013444.
HOGENOMiHOG000231715.
HOVERGENiHBG020688.
InParanoidiQ8K1B8.
KOiK17084.
OMAiEMMLFGA.
OrthoDBiEOG7T7GSC.
PhylomeDBiQ8K1B8.
TreeFamiTF314677.

Miscellaneous databases

PROiQ8K1B8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1B8.
CleanExiMM_FERMT3.
ExpressionAtlasiQ8K1B8. baseline and differential.
GenevisibleiQ8K1B8. MM.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 2 hits.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00295. B41. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00661. FERM_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  2. "The Kindlins: subcellular localization and expression during murine development."
    Ussar S., Wang H.-V., Linder S., Faessler R., Moser M.
    Exp. Cell Res. 312:3142-3151(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function."
    Krueger M., Moser M., Ussar S., Thievessen I., Luber C.A., Forner F., Schmidt S., Zanivan S., Faessler R., Mann M.
    Cell 134:353-364(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Kindlin-3 is essential for integrin activation and platelet aggregation."
    Moser M., Nieswandt B., Ussar S., Pozgajova M., Faessler R.
    Nat. Med. 14:325-330(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH ITGB1 AND ITGB3, MUTAGENESIS OF GLN-597.
  5. "Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells."
    Moser M., Bauer M., Schmid S., Ruppert R., Schmidt S., Sixt M., Wang H.-V., Sperandio M., Faessler R.
    Nat. Med. 15:300-305(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH ITGB2.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiURP2_MOUSE
AccessioniPrimary (citable) accession number: Q8K1B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.