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Protein

Complement component C8 alpha chain

Gene

C8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processComplement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-166665. Terminal pathway of complement.
R-MMU-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C8 alpha chain
Alternative name(s):
Complement component 8 subunit alpha
Gene namesi
Name:C8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2668347. C8a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Membrane attack complex, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002358721 – 30Sequence analysis10
ChainiPRO_000002358831 – 587Complement component C8 alpha chainAdd BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 74By similarity
Glycosylationi44C-linked (Man) tryptophanBy similarity1
Disulfide bondi50 ↔ 53By similarity
Disulfide bondi84 ↔ 90By similarity
Disulfide bondi96 ↔ 108By similarity
Disulfide bondi102 ↔ 121By similarity
Disulfide bondi115 ↔ 130By similarity
Disulfide bondi140 ↔ 177By similarity
Disulfide bondi194Interchain (with C-60 in C8-gamma chain)PROSITE-ProRule annotation
Disulfide bondi375 ↔ 399By similarity
Glycosylationi437N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi545C-linked (Man) tryptophanBy similarity1
Glycosylationi548C-linked (Man) tryptophanBy similarity1
Glycosylationi551C-linked (Man) tryptophanBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8K182.
PaxDbiQ8K182.
PeptideAtlasiQ8K182.
PRIDEiQ8K182.

PTM databases

iPTMnetiQ8K182.
PhosphoSitePlusiQ8K182.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035031.
CleanExiMM_C8A.
ExpressionAtlasiQ8K182. baseline and differential.
GenevisibleiQ8K182. MM.

Interactioni

Subunit structurei

Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b sequentially binds C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067541.

Structurei

3D structure databases

ProteinModelPortaliQ8K182.
SMRiQ8K182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 91TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini94 – 132LDL-receptor class APROSITE-ProRule annotationAdd BLAST39
Domaini136 – 498MACPFPROSITE-ProRule annotationAdd BLAST363
Domaini499 – 529EGF-likeAdd BLAST31
Domaini542 – 586TSP type-1 2PROSITE-ProRule annotationAdd BLAST45

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG410IE8U. Eukaryota.
ENOG410Y5MF. LUCA.
GeneTreeiENSGT00550000074478.
HOGENOMiHOG000231146.
HOVERGENiHBG005368.
InParanoidiQ8K182.
KOiK03997.
OMAiACRCGPC.
OrthoDBiEOG091G07LK.
PhylomeDBiQ8K182.
TreeFamiTF330498.

Family and domain databases

CDDicd00112. LDLa. 1 hit.
Gene3Di2.20.100.10. 1 hit.
3.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR016186. C-type_lectin-like/link_sf.
IPR036055. LDL_receptor-like_sf.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
IPR036383. TSP1_rpt_sf.
PfamiView protein in Pfam
PF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 1 hit.
PRINTSiPR00764. COMPLEMENTC9.
SMARTiView protein in SMART
SM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 2 hits.
SUPFAMiSSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiView protein in PROSITE
PS00022. EGF_1. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVVAFFGLS LVAWHPGVTA QEKVNQRVTR AVTPQAVSCQ LSDWYKWTDC
60 70 80 90 100
FPCQDKKYRY RSLLQPSKFG GTICSGDIWD EASCDSPTPC LRQAQCGQDF
110 120 130 140 150
QCRETGRCLK RHLVCNGDND CLDGSDESDC EDVRVTEDDC HQYEPIPGSE
160 170 180 190 200
RAALGYNILT QEEAQSVYDA KYYGGQCETV YNGDWRKLRY DPTCERLYYG
210 220 230 240 250
EDEKYFRKPY NFLKYHFEAL ADTSISSEFY DDANDLFFHI KNGKSHSAGV
260 270 280 290 300
TVGVAPVKSP VSIEVTGSGS KASSFLNKLN KYNEKRYGFM RVSTKIQTAQ
310 320 330 340 350
FKMRRNNIVL DEGMLQSLME LPEQFNYGMY AKFINDYGTH YITSGTMGGI
360 370 380 390 400
YEYVMVLDKE KMKTEGTTVD EVQKCIGGGI GIGIKDSTIE GVGISGEFCE
410 420 430 440 450
NSGDGDRDIR KKITGVEDII SRVQGGSSVW GSVLTHNSSA ITYQSWGRSL
460 470 480 490 500
KYNPVVIDFE MQPIYQLLRH TNLGPLETKR QNLRRALDQY LMEFNACRCG
510 520 530 540 550
PCFNNGEPIL DGTNCRCQCS MGRQGLACER TVIEGLKDFK AAGHWSCWSS
560 570 580
WSECRGGSQE RRRQCNNPPP KNGGTPCLGR NLQTQAC
Length:587
Mass (Da):66,080
Last modified:October 1, 2002 - v1
Checksum:iEEC529469F380A75
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti263I → V in strain: Mae/Stm. 1
Natural varianti468L → V in strain: BALB/c. 1
Natural varianti469R → Q in strain: Mae/Stm. 1
Natural varianti477E → R in strain: BALB/c; requires 2 nucleotide substitutions. 1
Natural varianti574 – 587GTPCL…QTQAC → DQHGGDF in strain: BALB/c. Add BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077295 mRNA. Translation: BAC41360.1.
AB077296 mRNA. Translation: BAC41361.1.
AB077298 mRNA. Translation: BAC41363.1.
BC027748 mRNA. Translation: AAH27748.1.
CCDSiCCDS18415.1.
RefSeqiNP_666260.1. NM_146148.2.
UniGeneiMm.197638.

Genome annotation databases

EnsembliENSMUST00000064873; ENSMUSP00000067541; ENSMUSG00000035031.
GeneIDi230558.
KEGGimmu:230558.
UCSCiuc008tya.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCO8A_MOUSE
AccessioniPrimary (citable) accession number: Q8K182
Secondary accession number(s): Q8CHJ9, Q8CHK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 1, 2002
Last modified: November 22, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families