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Q8K126 (RF1M_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 1, mitochondrial

Short name=MRF-1
Short name=MtRF-1
Gene names
Name:Mtrf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA By similarity.

Subcellular location

Mitochondrion.

Post-translational modification

Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals By similarity.

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrion

Inferred from direct assay PubMed 14651853PubMed 18614015. Source: MGI

   Molecular_functiontranslation release factor activity, codon specific

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 446Peptide chain release factor 1, mitochondrialPRO_0000030334

Amino acid modifications

Modified residue3141N5-methylglutamine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K126 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: FC25AFF242025DE5

FASTA44652,458
        10         20         30         40         50         60 
MSHHLCIWLF RNPFLRACPQ RHVFLSCQQF RQISLDTRPW NFRQKKTHVL YQLLNKSWSR 

        70         80         90        100        110        120 
GCCHQGTRKL WKHKALQKYM EDLNKEYQTL DQCLQGISEN EGDRRALHRR HAQLAPLAAV 

       130        140        150        160        170        180 
YQEIQEAEQA IEELESLCKS LNKQDEKQLQ ELVSEERQII DQKIHRLYSE LLERLVPKEK 

       190        200        210        220        230        240 
YDWSDVILEV TSGRTTGGDI CQQFTREIFD MYQNYSYYKH WKFELLNYTP ADYGGLHHAA 

       250        260        270        280        290        300 
ARISGDSVYK HLKYEGGIHR VQRIPEVGLS SRMQRIHTGT MSVIVLPQPD EVDVKVDPKD 

       310        320        330        340        350        360 
LRVDTFRARG AGGQHVNTTD SAVRLVHIPT GLVVECQQER SQLKNKEIAL RVLRARLYQQ 

       370        380        390        400        410        420 
IIEKDRCQQQ NARKLQVGTR AQSERIRTYN FTQDRVTDHR IAYEVRDIKE FLRGEKCLDQ 

       430        440 
LIERLLQSAD EEAISEFLDE SLQSVK 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain cortex.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK043981 mRNA. Translation: BAC31724.1.
BC028898 mRNA. Translation: AAH28898.1.
RefSeqNP_666072.3. NM_145960.4.
UniGeneMm.35306.

3D structure databases

ProteinModelPortalQ8K126.
SMRQ8K126. Positions 97-438.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K126.

Proteomic databases

PaxDbQ8K126.
PRIDEQ8K126.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022600; ENSMUSP00000022600; ENSMUSG00000022022.
GeneID211253.
KEGGmmu:211253.
UCSCuc007usw.1. mouse.

Organism-specific databases

CTD9617.
MGIMGI:2384815. Mtrf1.

Phylogenomic databases

eggNOGCOG0216.
GeneTreeENSGT00390000011879.
HOGENOMHOG000074815.
HOVERGENHBG017071.
InParanoidQ8K126.
KOK02835.
OMAIKEFLCG.
OrthoDBEOG7DRJ33.
PhylomeDBQ8K126.
TreeFamTF313720.

Gene expression databases

BgeeQ8K126.
CleanExMM_MTRF1.
GenevestigatorQ8K126.

Family and domain databases

Gene3D3.30.160.20. 1 hit.
InterProIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTSM00937. PCRF. 1 hit.
[Graphical view]
PROSITEPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio373184.
PROQ8K126.
SOURCESearch...

Entry information

Entry nameRF1M_MOUSE
AccessionPrimary (citable) accession number: Q8K126
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot