Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CCR4-NOT transcription complex subunit 3

Gene

Cnot3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 3
Alternative name(s):
CCR4-associated factor 3
Gene namesi
Name:Cnot3
Synonyms:Not3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385261. Cnot3.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT complex Source: UniProtKB
  • CCR4-NOT core complex Source: GO_Central
  • cytoplasmic mRNA processing body Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 751751CCR4-NOT transcription complex subunit 3PRO_0000198334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei292 – 2921PhosphothreonineBy similarity
Modified residuei299 – 2991PhosphoserineCombined sources
Modified residuei540 – 5401PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K0V4.
MaxQBiQ8K0V4.
PaxDbiQ8K0V4.
PeptideAtlasiQ8K0V4.
PRIDEiQ8K0V4.

PTM databases

iPTMnetiQ8K0V4.
PhosphoSiteiQ8K0V4.

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells and in inner cell mass (ICM) of the blastocyst.1 Publication

Inductioni

Decreased levels in liver and white adipose tissues upon fasting,.1 Publication

Gene expression databases

BgeeiENSMUSG00000035632.
CleanExiMM_CNOT3.
ExpressionAtlasiQ8K0V4. baseline and differential.
GenevisibleiQ8K0V4. MM.

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.1 Publication

Protein-protein interaction databases

BioGridi231295. 18 interactions.
DIPiDIP-46844N.
IntActiQ8K0V4. 21 interactions.
MINTiMINT-4607034.
STRINGi10090.ENSMUSP00000039098.

Structurei

3D structure databases

ProteinModelPortaliQ8K0V4.
SMRiQ8K0V4. Positions 605-746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni659 – 75193Repressor domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi314 – 39582Pro-richAdd
BLAST
Compositional biasi396 – 511116Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

eggNOGiKOG2150. Eukaryota.
COG5665. LUCA.
GeneTreeiENSGT00390000014743.
HOGENOMiHOG000184850.
HOVERGENiHBG051042.
InParanoidiQ8K0V4.
KOiK12580.
OMAiCEWISSA.
OrthoDBiEOG091G040M.
PhylomeDBiQ8K0V4.
TreeFamiTF321963.

Family and domain databases

InterProiIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K0V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK
60 70 80 90 100
EIKKLQRLRD QIKTWVASNE IKDKRQLIEN RKLIETQMER FKVVERETKT
110 120 130 140 150
KAYSKEGLGL AQKVDPAQKE KEEVGQWLTN TIDTLNMQVD QFESEVESLS
160 170 180 190 200
VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV RMLETILRML DNDSILVDAI
210 220 230 240 250
RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA TSPPSHSHME
260 270 280 290 300
DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP
310 320 330 340 350
AKNGSKPVHS NQHPQSPAVP PTYPSGPPPT TSALSSTPGN NGASTPAAPT
360 370 380 390 400
SALGPKASPA PSHNSGTPAP YAQAVAPPNA SGPSNAQPRP PSAQPSGGSG
410 420 430 440 450
GGSGGSSSNS NSGTGGGAGK QNGATSYSSV VADSPAEVTL SSSGGSSASS
460 470 480 490 500
QALGPTSGPH NPAPSTSKES STAAPSGAGN VASGSGNNSG GPSLLVPLPV
510 520 530 540 550
NPPSSPTPSF SEAKAAGTLL NGPPQFSTTP EIKAPEPLSS LKSMAERAAI
560 570 580 590 600
SSGIEDPVPT LHLTDRDIIL SSTSAPPTSS QPPLQLSEVN IPLSLGVCPL
610 620 630 640 650
GPVSLTKEQL YQQAMEEAAW HHMPHPSDSE RIRQYLPRNP CPTPPYHHQM
660 670 680 690 700
PPPHSDTVEF YQRLSTETLF FIFYYLEGTK AQYLAAKALK KQSWRFHTKY
710 720 730 740 750
MMWFQRHEEP KTITDEFEQG TYIYFDYEKW GQRKKEGFTF EYRYLEDRDL

Q
Length:751
Mass (Da):81,946
Last modified:October 1, 2002 - v1
Checksum:i47C2A7321EDAB13A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC030332 mRNA. Translation: AAH30332.1.
BC053437 mRNA. Translation: AAH53437.1.
BC141041 mRNA. Translation: AAI41042.1.
CCDSiCCDS20721.1.
RefSeqiNP_666288.1. NM_146176.3.
XP_011248803.1. XM_011250501.1.
UniGeneiMm.358731.

Genome annotation databases

EnsembliENSMUST00000038913; ENSMUSP00000039098; ENSMUSG00000035632.
GeneIDi232791.
KEGGimmu:232791.
UCSCiuc009evl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC030332 mRNA. Translation: AAH30332.1.
BC053437 mRNA. Translation: AAH53437.1.
BC141041 mRNA. Translation: AAI41042.1.
CCDSiCCDS20721.1.
RefSeqiNP_666288.1. NM_146176.3.
XP_011248803.1. XM_011250501.1.
UniGeneiMm.358731.

3D structure databases

ProteinModelPortaliQ8K0V4.
SMRiQ8K0V4. Positions 605-746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231295. 18 interactions.
DIPiDIP-46844N.
IntActiQ8K0V4. 21 interactions.
MINTiMINT-4607034.
STRINGi10090.ENSMUSP00000039098.

PTM databases

iPTMnetiQ8K0V4.
PhosphoSiteiQ8K0V4.

Proteomic databases

EPDiQ8K0V4.
MaxQBiQ8K0V4.
PaxDbiQ8K0V4.
PeptideAtlasiQ8K0V4.
PRIDEiQ8K0V4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038913; ENSMUSP00000039098; ENSMUSG00000035632.
GeneIDi232791.
KEGGimmu:232791.
UCSCiuc009evl.2. mouse.

Organism-specific databases

CTDi4849.
MGIiMGI:2385261. Cnot3.

Phylogenomic databases

eggNOGiKOG2150. Eukaryota.
COG5665. LUCA.
GeneTreeiENSGT00390000014743.
HOGENOMiHOG000184850.
HOVERGENiHBG051042.
InParanoidiQ8K0V4.
KOiK12580.
OMAiCEWISSA.
OrthoDBiEOG091G040M.
PhylomeDBiQ8K0V4.
TreeFamiTF321963.

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Miscellaneous databases

PROiQ8K0V4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035632.
CleanExiMM_CNOT3.
ExpressionAtlasiQ8K0V4. baseline and differential.
GenevisibleiQ8K0V4. MM.

Family and domain databases

InterProiIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT3_MOUSE
AccessioniPrimary (citable) accession number: Q8K0V4
Secondary accession number(s): B2RUA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.