Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Reticulon-4 receptor-like 1

Gene

Rtn4rl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface receptor that plays a functionally redundant role in postnatal brain development and in regulating axon regeneration in the adult central nervous system (PubMed:22406547, PubMed:27339102). Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum (PubMed:27339102). Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG (PubMed:26335717). Plays a role in inhibiting neurite outgrowth and axon regeneration via its binding to neuronal chondroitin sulfate proteoglycans (PubMed:22406547). Binds heparin (PubMed:22406547). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:22325200).4 Publications

GO - Molecular functioni

  • chondroitin sulfate binding Source: UniProtKB
  • heparin binding Source: UniProtKB
  • protein kinase inhibitor activity Source: GO_Central
  • receptor activity Source: UniProtKB

GO - Biological processi

  • corpus callosum development Source: UniProtKB
  • cytokine-mediated signaling pathway Source: GO_Central
  • negative regulation of axon regeneration Source: UniProtKB
  • negative regulation of JAK-STAT cascade Source: GO_Central
  • negative regulation of neuron projection development Source: UniProtKB
  • negative regulation of protein kinase activity Source: GO_Central

Keywordsi

Molecular functionHeparin-binding, Receptor

Enzyme and pathway databases

ReactomeiR-MMU-163125. Post-translational modification: synthesis of GPI-anchored proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Reticulon-4 receptor-like 1
Alternative name(s):
Nogo receptor-like 2
Nogo-66 receptor homolog 2
Nogo-66 receptor-related protein 3
Short name:
NgR31 Publication
Gene namesi
Name:Rtn4rl1Imported
Synonyms:Ngrl2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2661375. Rtn4rl1.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor By similarity
  • Membrane raft By similarity
  • Perikaryon By similarity
  • Cell projection By similarity

  • Note: Localized to the surface of neurons, including axons.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:22406547). Mice are born at the expected Mendelian rate, are viable and fertile (PubMed:22406547). Compared to wild-type littermates, cultured hippocampus neurons from mutant mice display an increased number of excitatory synapses (PubMed:22325200). Likewise, mice with a triple gene disruption that lack Rtn4r, Rtn4rl1 and Rtn4rl2 have no visible phenotype, are healthy and viable (PubMed:22406547, PubMed:22325200). Mice with a triple gene disruption that lack Rtn4r, Rtn4rl1 and Rtn4rl2 have normal brain size and grossly normal brain anatomy, but display defects of medial brain structures, including an absence of the fasciola cinereum, corpus callosum agenesis and formation of bilateral Probst bundles indicative of the failure of callosally projecting neurons to extend across the midline (PubMed:27339102). Mice with a triple gene disruption of Rtn4r, Rtn4rl1 and Rtn4rl2 display impaired ability to stay on a rotarod and increased spontaneous locomotion (PubMed:27339102). These mice display an increased number of excitatory synapses in the apical dendritic regions of hippocampus neurons, an increase in the complexity of dendrite structure and increased total dendrite length (PubMed:22325200). One month after birth, mice with a triple gene disruption that lack Rtn4r, Rtn4rl1 and Rtn4rl2 show a significant reduction in the survival of motoneurons (PubMed:26335717). Compared to wild-type or single mutants, cerebellar granule cells from mice lacking Rtn4r, Rtn4rl1 and Rtn4rl2 show decreased myelin-mediated inhibition of neurite outgrowth, an inhibition that is strongly decreased on myelin deficient in Mag, Rtn4 and Omg (PubMed:22406547). Mice lacking both Rtn4r and Rtn4rl1 show increased axon regeneration after injury; the same effect is observed when Rtn4r, Rtn4rl1 and Rtn4rl2 are disrupted (PubMed:22406547). Combined disruption of Rtn4r, Rtn4rl1 and Ptprs further increases axon regeneration after injury (PubMed:22406547). Single gene disruption of Rtn4r, Rtn4rl1 and Rtn4rl2 and combined disruption of Rtn4r and Rtn4rl2 have no effect on axon regeneration (PubMed:22406547).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000004604425 – 424Reticulon-4 receptor-like 1Add BLAST400
PropeptideiPRO_0000046045425 – 445Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi424GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ8K0S5.
PaxDbiQ8K0S5.
PeptideAtlasiQ8K0S5.
PRIDEiQ8K0S5.

PTM databases

iPTMnetiQ8K0S5.
PhosphoSitePlusiQ8K0S5.

Expressioni

Tissue specificityi

Detected in brain (at protein level) (PubMed:22406547). Detected in retina ganglion cell layer and inner nuclear layer (PubMed:22406547).1 Publication

Developmental stagei

At E13.5, strongly expressed in PNS ganglia and developing heart, and weakly expressed in brain and spinal cord. By postnatal day 1, strongly expressed in dorsal root ganglia and in dorsal and gray matter areas of spinal cord. Expressed in various adult brain structures including the amygdala, caudate putamen, cerebellum, cerebral cortex, hippocampus, olfactory bulb and thalamus.1 Publication

Gene expression databases

BgeeiENSMUSG00000045287.
CleanExiMM_RTN4RL1.
GenevisibleiQ8K0S5. MM.

Interactioni

Subunit structurei

Identified in a complex that contains RTN4R, RTN4RL1 and NGFR; the interaction depends on the presence of chondroitin sulfate proteoglycans (PubMed:22406547). Does not interact with MAG, OMG and RTN4 (PubMed:12839991).2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099572.

Structurei

3D structure databases

ProteinModelPortaliQ8K0S5.
SMRiQ8K0S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 54LRRNTAdd BLAST30
Repeati55 – 76LRR 1Add BLAST22
Repeati77 – 98LRR 2Add BLAST22
Repeati101 – 123LRR 3Add BLAST23
Repeati126 – 147LRR 4Add BLAST22
Repeati150 – 171LRR 5Add BLAST22
Repeati174 – 195LRR 6Add BLAST22
Repeati198 – 219LRR 7Add BLAST22
Repeati222 – 243LRR 8Add BLAST22
Domaini255 – 306LRRCTAdd BLAST52

Sequence similaritiesi

Belongs to the Nogo receptor family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00870000136383.
HOGENOMiHOG000116109.
HOVERGENiHBG063707.
InParanoidiQ8K0S5.
KOiK16660.
OMAiNCTSHRN.
OrthoDBiEOG091G08II.
PhylomeDBiQ8K0S5.
TreeFamiTF330080.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiView protein in InterPro
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
PfamiView protein in Pfam
PF13855. LRR_8. 3 hits.
SMARTiView protein in SMART
SM00369. LRR_TYP. 7 hits.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiView protein in PROSITE
PS51450. LRR. 6 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K0S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRKGCCVEL LLLLLAGELP LGGGCPRDCV CYPAPMTVSC QAHNFAAIPE
60 70 80 90 100
GIPEDSERIF LQNNRITFLQ QGHFSPAMVT LWIYSNNITF IAPNTFEGFV
110 120 130 140 150
HLEELDLGDN RQLRTLAPET FQGLVKLHAL YLYKCGLSAL PAGIFGGLHS
160 170 180 190 200
LQYLYLQDNH IEYLQDDIFV DLVNLSHLFL HGNKLWSLGQ GIFRGLVNLD
210 220 230 240 250
RLLLHENQLQ WVHHKAFHDL HRLTTLFLFN NSLTELQGDC LAPLVALEFL
260 270 280 290 300
RLNGNAWDCG CRARSLWEWL RRFRGSSSAV PCATPELRQG QDLKLLRVED
310 320 330 340 350
FRNCTGPVSP HQIKSHTLTT SDRAARKEHH PSHGASRDKG HPHGHPPGSR
360 370 380 390 400
SGYKKAGKNC TSHRNRNQIS KVSSGKELTE LQDYAPDYQH KFSFDIMPTA
410 420 430 440
RPKRKGKCAR RTPIRAPSGV QQASSGTALG APLLAWILGL AVTLR
Length:445
Mass (Da):49,836
Last modified:October 1, 2002 - v1
Checksum:i8285A01C1250D18A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY250218 mRNA. Translation: AAP82835.1.
AK033286 mRNA. Translation: BAC28223.1.
AK140456 mRNA. Translation: BAE24396.1.
AL603905 Genomic DNA. Translation: CAI35092.1.
BC030471 mRNA. Translation: AAH30471.1.
BK001304 mRNA. Translation: DAA01387.1.
CCDSiCCDS25043.1.
RefSeqiNP_808376.1. NM_177708.5.
UniGeneiMm.82661.

Genome annotation databases

EnsembliENSMUST00000102514; ENSMUSP00000099572; ENSMUSG00000045287.
GeneIDi237847.
KEGGimmu:237847.
UCSCiuc007kdh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY250218 mRNA. Translation: AAP82835.1.
AK033286 mRNA. Translation: BAC28223.1.
AK140456 mRNA. Translation: BAE24396.1.
AL603905 Genomic DNA. Translation: CAI35092.1.
BC030471 mRNA. Translation: AAH30471.1.
BK001304 mRNA. Translation: DAA01387.1.
CCDSiCCDS25043.1.
RefSeqiNP_808376.1. NM_177708.5.
UniGeneiMm.82661.

3D structure databases

ProteinModelPortaliQ8K0S5.
SMRiQ8K0S5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099572.

PTM databases

iPTMnetiQ8K0S5.
PhosphoSitePlusiQ8K0S5.

Proteomic databases

MaxQBiQ8K0S5.
PaxDbiQ8K0S5.
PeptideAtlasiQ8K0S5.
PRIDEiQ8K0S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102514; ENSMUSP00000099572; ENSMUSG00000045287.
GeneIDi237847.
KEGGimmu:237847.
UCSCiuc007kdh.1. mouse.

Organism-specific databases

CTDi146760.
MGIiMGI:2661375. Rtn4rl1.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00870000136383.
HOGENOMiHOG000116109.
HOVERGENiHBG063707.
InParanoidiQ8K0S5.
KOiK16660.
OMAiNCTSHRN.
OrthoDBiEOG091G08II.
PhylomeDBiQ8K0S5.
TreeFamiTF330080.

Enzyme and pathway databases

ReactomeiR-MMU-163125. Post-translational modification: synthesis of GPI-anchored proteins.

Miscellaneous databases

PROiPR:Q8K0S5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045287.
CleanExiMM_RTN4RL1.
GenevisibleiQ8K0S5. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiView protein in InterPro
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
PfamiView protein in Pfam
PF13855. LRR_8. 3 hits.
SMARTiView protein in SMART
SM00369. LRR_TYP. 7 hits.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiView protein in PROSITE
PS51450. LRR. 6 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiR4RL1_MOUSE
AccessioniPrimary (citable) accession number: Q8K0S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: May 10, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.