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Q8K0L9 (ZBT20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 20
Alternative name(s):
BTB/POZ domain zinc finger factor HOF
Zinc finger protein 288
Gene names
Name:Zbtb20
Synonyms:Zfp288
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses By similarity. Binds to DNA.

Subunit structure

Can homodimerize. Binds to DNA. Ref.1

Subcellular location

Nucleus Probable Ref.1.

Tissue specificity

Specifically expressed in early hippocampal neurons, cerebellar granule cells and gliogenic progenitors as well as in differentiated glia. Ref.1

Post-translational modification

Sumoylated with SUMO1. Ref.6

Sequence similarities

Contains 1 BTB (POZ) domain.

Contains 5 C2H2-type zinc fingers.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K0L9-1)

Also known as: HOF-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K0L9-2)

Also known as: HOF-S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 741741Zinc finger and BTB domain-containing protein 20
PRO_0000325961

Regions

Domain104 – 16764BTB
Zinc finger578 – 60023C2H2-type 1
Zinc finger606 – 62823C2H2-type 2
Zinc finger634 – 65623C2H2-type 3
Zinc finger662 – 68423C2H2-type 4
Zinc finger715 – 73723C2H2-type 5

Amino acid modifications

Modified residue4651Phosphoserine By similarity
Modified residue4721Phosphoserine By similarity

Natural variations

Alternative sequence1 – 7373Missing in isoform 2.
VSP_032504

Experimental info

Sequence conflict881N → D in BAB23755. Ref.2
Sequence conflict4211A → D in AAF06015. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (HOF-L) [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 04C0744D66BFE418

FASTA74181,034
        10         20         30         40         50         60 
MLERKKPKTA ENQKASEENE ITQPGGSSAK PALPCLNFEA VLSPAPALIH STHSLTNSHA 

        70         80         90        100        110        120 
HTGSSDCDIS CKGMTERIHS INLHNFSNSV LETLNEQRNR GHFCDVTVRI HGSMLRAHRC 

       130        140        150        160        170        180 
VLAAGSPFFQ DKLLLGYSDI EIPSVVSVQS VQKLIDFMYS GVLRVSQSEA LQILTAASIL 

       190        200        210        220        230        240 
QIKTVIDECT RIVSQNVGDV FPGIQDSGQD TPRGTPESGT SGQSSDTESG YLQSHPQHSV 

       250        260        270        280        290        300 
DRIYSALYAC SMQNGSGERS FYSGAVVSHH ETALGLPRDH HMEDPSWITR IHERSQQMER 

       310        320        330        340        350        360 
YLSTTPETTH CRKQPRPVRI QTLVGNIHIK QEMEDDYDYY GQQRVQILER NESEECTEDT 

       370        380        390        400        410        420 
DQAEGTESEP KGESFDSGVS SSIGTEPDSV EQQFGAAAPR DGQAEPAQPE QAAEAPAESS 

       430        440        450        460        470        480 
AQPNQLEPGA SSPERSNESE MDNTVITVSN SSDKGVLQQP SVNTSIGQPL PSTQLYLRQT 

       490        500        510        520        530        540 
ETLTSNLRMP LTLTSNTQVI GTAGNTYLPA LFTTQPAGSG PKPFLFSLPQ PLTGQQTQFV 

       550        560        570        580        590        600 
TVSQPGLSTF TAQLPAPQPL ASSAGHSTAS GQGDKKPYEC TLCNKTFTAK QNYVKHMFVH 

       610        620        630        640        650        660 
TGEKPHQCSI CWRSFSLKDY LIKHMVTHTG VRAYQCSICN KRFTQKSSLN VHMRLHRGEK 

       670        680        690        700        710        720 
SYECYICKKK FSHKTLLERH VALHSASNGT PPAGTPPGAR AGPPGVVACT EGTTYVCSVC 

       730        740 
PAKFDQIEQF NDHMRMHVSD G 

« Hide

Isoform 2 (HOF-S) [UniParc].

Checksum: 5A6FF5BEEA3963A3
Show »

FASTA66873,382

References

« Hide 'large scale' references
[1]"Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia."
Mitchelmore C., Kjaerulff K.M., Pedersen H.C., Nielsen J.V., Rasmussen T.E., Fisker M.F., Finsen B., Pedersen K.M., Jensen N.A.
J. Biol. Chem. 277:7598-7609(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, HOMODIMERIZATION, TISSUE SPECIFICITY.
Tissue: Oligodendroglioma.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Liver.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Kidney.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice."
Tirard M., Hsiao H.H., Nikolov M., Urlaub H., Melchior F., Brose N.
Proc. Natl. Acad. Sci. U.S.A. 109:21122-21127(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION WITH SUMO1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF194030 mRNA. Translation: AAF06015.1.
AK005028 mRNA. Translation: BAB23755.1.
CT010512 Genomic DNA. Translation: CAO77851.1.
CT010512 Genomic DNA. Translation: CAO77852.1.
BC031114 mRNA. Translation: AAH31114.1.
BC056446 mRNA. Translation: AAH56446.1.
CCDSCCDS28178.1. [Q8K0L9-1]
CCDS49851.1. [Q8K0L9-2]
RefSeqNP_001272734.1. NM_001285805.1. [Q8K0L9-1]
NP_062752.2. NM_019778.2. [Q8K0L9-1]
NP_851401.1. NM_181058.1. [Q8K0L9-2]
XP_006522475.1. XM_006522412.1. [Q8K0L9-1]
XP_006522476.1. XM_006522413.1. [Q8K0L9-1]
XP_006522477.1. XM_006522414.1. [Q8K0L9-1]
XP_006522478.1. XM_006522415.1. [Q8K0L9-1]
XP_006522479.1. XM_006522416.1. [Q8K0L9-1]
XP_006522480.1. XM_006522417.1. [Q8K0L9-1]
XP_006522481.1. XM_006522418.1. [Q8K0L9-1]
XP_006522482.1. XM_006522419.1. [Q8K0L9-2]
XP_006522483.1. XM_006522420.1. [Q8K0L9-2]
XP_006522484.1. XM_006522421.1. [Q8K0L9-2]
XP_006522485.1. XM_006522422.1. [Q8K0L9-2]
XP_006522486.1. XM_006522423.1. [Q8K0L9-2]
XP_006522487.1. XM_006522424.1. [Q8K0L9-2]
UniGeneMm.136238.
Mm.441121.
Mm.441972.
Mm.460947.
Mm.491236.

3D structure databases

ProteinModelPortalQ8K0L9.
SMRQ8K0L9. Positions 81-190, 529-737.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8K0L9. 1 interaction.

Proteomic databases

PaxDbQ8K0L9.
PRIDEQ8K0L9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000079441; ENSMUSP00000078410; ENSMUSG00000022708. [Q8K0L9-1]
ENSMUST00000114690; ENSMUSP00000110338; ENSMUSG00000022708. [Q8K0L9-2]
ENSMUST00000114691; ENSMUSP00000110339; ENSMUSG00000022708. [Q8K0L9-2]
ENSMUST00000114694; ENSMUSP00000110342; ENSMUSG00000022708. [Q8K0L9-1]
ENSMUST00000114695; ENSMUSP00000110343; ENSMUSG00000022708. [Q8K0L9-1]
GeneID56490.
KEGGmmu:56490.
UCSCuc007zga.2. mouse. [Q8K0L9-1]

Organism-specific databases

CTD26137.
MGIMGI:1929213. Zbtb20.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00750000117519.
HOGENOMHOG000136747.
HOVERGENHBG060183.
InParanoidQ8K0L9.
KOK10501.
OMAQIEHFND.
OrthoDBEOG7GQXV7.
PhylomeDBQ8K0L9.
TreeFamTF335684.

Gene expression databases

ArrayExpressQ8K0L9.
BgeeQ8K0L9.
CleanExMM_ZBTB20.
GenevestigatorQ8K0L9.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZBTB20. mouse.
NextBio312770.
PROQ8K0L9.
SOURCESearch...

Entry information

Entry nameZBT20_MOUSE
AccessionPrimary (citable) accession number: Q8K0L9
Secondary accession number(s): A6X916, Q9DBD4, Q9QZ87
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot