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Protein

Acyl-coenzyme A synthetase ACSM2, mitochondrial

Gene

Acsm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) (By similarity).By similarity

Catalytic activityi

ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity, Mn2+By similarity

Enzyme regulationi

Activated by monovalent cations, such as potassium, rubidium or ammonium.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei139Coenzyme ABy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei447ATPBy similarity1
Binding sitei462ATPBy similarity1
Binding sitei473SubstrateBy similarity1
Binding sitei502Coenzyme ABy similarity1
Binding sitei533Coenzyme ABy similarity1
Binding sitei558ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 229ATPBy similarity9
Nucleotide bindingi360 – 365ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-177128 Conjugation of salicylate with glycine
R-MMU-177135 Conjugation of benzoate with glycine
R-MMU-177162 Conjugation of phenylacetate with glutamine

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A synthetase ACSM2, mitochondrial (EC:6.2.1.2)
Alternative name(s):
Acyl-CoA synthetase medium-chain family member 2
Butyrate--CoA ligase 2
Butyryl-coenzyme A synthetase 2
Middle-chain acyl-CoA synthetase 2
Gene namesi
Name:Acsm2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385289 Acsm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46MitochondrionSequence analysisAdd BLAST46
ChainiPRO_000030609547 – 575Acyl-coenzyme A synthetase ACSM2, mitochondrialAdd BLAST529

Proteomic databases

PaxDbiQ8K0L3
PeptideAtlasiQ8K0L3
PRIDEiQ8K0L3

PTM databases

iPTMnetiQ8K0L3
PhosphoSitePlusiQ8K0L3

Expressioni

Gene expression databases

BgeeiENSMUSG00000030945
CleanExiMM_ACSM2
ExpressionAtlasiQ8K0L3 baseline and differential
GenevisibleiQ8K0L3 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi231445, 1 interactor
IntActiQ8K0L3, 2 interactors
MINTiQ8K0L3
STRINGi10090.ENSMUSP00000095690

Structurei

3D structure databases

ProteinModelPortaliQ8K0L3
SMRiQ8K0L3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni470 – 472Coenzyme A bindingBy similarity3
Regioni541 – 543Coenzyme A bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175 Eukaryota
COG0365 LUCA
GeneTreeiENSGT00760000119178
HOGENOMiHOG000229982
HOVERGENiHBG053031
InParanoidiQ8K0L3
KOiK01896
OMAiGCRYLDD
OrthoDBiEOG091G03NW
PhylomeDBiQ8K0L3
TreeFamiTF354287

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K0L3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHHLWKIPRL FTLWGNEISC RTFHMNIKKL IPIQWGHQEA PAKFNFASDV
60 70 80 90 100
IDHWASVEKA GKRSSGPALW WMNGSGKEIK WSFRELSEAS KQTANVLSGA
110 120 130 140 150
CGLHRGDRVA VVLPRIPEWW LMILGCMRTG LVFMPGTIQM RSSDILYRLQ
160 170 180 190 200
ASKARAIVAG DEVAQEVDAV APDCSFLKIK LLVSENSREG WLNFKALLKE
210 220 230 240 250
ASTIHQCVET ESRESAAIYF TSGTSGPPKM AEHSHCSLGI KAKMDAASWT
260 270 280 290 300
GLSTSDIIWT ISDTAWIMNI LGAFLEPWVL GACIFVHLLP KFDSQTVLKV
310 320 330 340 350
LSSYPINTLV GAPIIYRMLL QQDLSSYKFP HLHSCFSGGE TLLPETLENW
360 370 380 390 400
KAKTGLEIRE IYGQTETGLI CRVSRTMKVK PGYLGTAFAH YDVQVIDEQG
410 420 430 440 450
NVLPPGKEGD IAIRVKPIWP IGMFSGYVDN PKKTQDNIRG DFWLMGDRGI
460 470 480 490 500
KDPEGYFHFI GRSDDIINSS GYRIGPSEVE NALMEHPAVS ETAVISSPDP
510 520 530 540 550
SRGEVVKAFV VLAPEFLSHD RDQLTKVLQE HVKSVTAPYK YPRKVEFVLD
560 570
LPKTVTGKIE RAKLRAKEWK TSGRA
Length:575
Mass (Da):64,269
Last modified:October 1, 2002 - v1
Checksum:iA7E5956D54B119D8
GO
Isoform 2 (identifier: Q8K0L3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTTGSLDLPFVGSHRWIKWTTASLTM

Note: No experimental confirmation available.
Show »
Length:600
Mass (Da):67,057
Checksum:iCD0F661EC08E1C4F
GO
Isoform 3 (identifier: Q8K0L3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     471-471: G → GAYFCR

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):64,910
Checksum:i2D228B445D3D2DF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti515E → G in BAB71542 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0283931M → MTTGSLDLPFVGSHRWIKWT TASLTM in isoform 2. 1 Publication1
Alternative sequenceiVSP_028394471G → GAYFCR in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140827 mRNA Translation: BAE24490.1
AK143757 mRNA Translation: BAE25526.1
AK165353 mRNA Translation: BAE38146.1
AK165401 mRNA Translation: BAE38163.1
AK057650 mRNA Translation: BAB71542.1
BC024424 mRNA Translation: AAH24424.1
BC031140 mRNA Translation: AAH31140.1
CCDSiCCDS21782.1 [Q8K0L3-1]
CCDS52379.1 [Q8K0L3-2]
CCDS52380.1 [Q8K0L3-3]
RefSeqiNP_001171448.1, NM_001177977.1 [Q8K0L3-2]
NP_001171449.1, NM_001177978.1 [Q8K0L3-3]
NP_666309.1, NM_146197.4 [Q8K0L3-1]
UniGeneiMm.268448

Genome annotation databases

EnsembliENSMUST00000084647; ENSMUSP00000081697; ENSMUSG00000030945 [Q8K0L3-3]
ENSMUST00000098084; ENSMUSP00000095690; ENSMUSG00000030945 [Q8K0L3-2]
ENSMUST00000167935; ENSMUSP00000126670; ENSMUSG00000030945 [Q8K0L3-1]
GeneIDi233799
KEGGimmu:233799
UCSCiuc009jlh.2 mouse [Q8K0L3-1]
uc009jli.2 mouse [Q8K0L3-3]
uc009jlj.2 mouse [Q8K0L3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACSM2_MOUSE
AccessioniPrimary (citable) accession number: Q8K0L3
Secondary accession number(s): Q3TNC6
, Q3US47, Q8R1L3, Q96LX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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