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Q8K0L2 (ENTP8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ectonucleoside triphosphate diphosphohydrolase 8

Short name=E-NTPDase 8
Short name=NTPDase 8
Short name=NTPDase8
EC=3.6.1.5
Gene names
Name:Entpd8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. Ref.1

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate. Ref.1

Cofactor

Ca2+ or Mg2+. Has lower efficiency with Mg2+. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in liver, jejunum and kidney. Ref.1

Domain

The transmembranous domains are involved in regulation of enzyme activity By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Biophysicochemical properties

Kinetic parameters:

KM=13 µM for ATP Ref.1

KM=41 µM for ADP

KM=47 µM for UTP

KM=171 µM for UDP

pH dependence:

Optimum pH is 5.5-8.0.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K0L2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K0L2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     263-265: Missing.
     353-389: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497Ectonucleoside triphosphate diphosphohydrolase 8
PRO_0000306883

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 2921Helical; Potential
Topological domain30 – 473444Extracellular Potential
Transmembrane474 – 49421Helical; Potential
Topological domain495 – 4973Cytoplasmic Potential

Sites

Active site1681Proton acceptor By similarity

Amino acid modifications

Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Disulfide bond78 ↔ 102 By similarity
Disulfide bond245 ↔ 294 By similarity
Disulfide bond331 ↔ 337 By similarity
Disulfide bond383 ↔ 405 By similarity

Natural variations

Alternative sequence263 – 2653Missing in isoform 2.
VSP_028560
Alternative sequence353 – 38937Missing in isoform 2.
VSP_028561

Experimental info

Sequence conflict4371D → N in AAH31143. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 3.
Checksum: 00DE822B6EEB1BDF

FASTA49754,650
        10         20         30         40         50         60 
MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG SSHTSLFVYQ 

        70         80         90        100        110        120 
WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK SCLEEALALI PQAQHPETPT 

       130        140        150        160        170        180 
FLGSTAGMRL LSQKNSSQAR DILAAVSQTL SKSPVDFWGA KILAGQDEGA FGWITINYVL 

       190        200        210        220        230        240 
GMLLKYSSGQ WILPEEGMLV GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT 

       250        260        270        280        290        300 
HSYLCFGRDQ ILNRLLAKLA QDRLSSQVAP VRHPCYHSGY QAILPLSSLY DSPCIHTTDS 

       310        320        330        340        350        360 
LNHTQNLTVE GTGDPGNCVV ALRSLFNFSS CKGQKDCAFN GIYQPPVHGQ FYAFSNFYYT 

       370        380        390        400        410        420 
FHFLNLTSRQ SLNTVNDTVW KFCQKPWKLV EVSYPGQERW LRDYCASGLY ILVLLLEGYK 

       430        440        450        460        470        480 
FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL TGMIPAEAPT HWRAQSYSIW TAGVVFAVLT 

       490 
LVAILGAAAI QIFWTQD 

« Hide

Isoform 2 [UniParc].

Checksum: 9A5BBDF66294C1CB
Show »

FASTA45749,766

References

« Hide 'large scale' references
[1]"Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8."
Bigonnesse F., Levesque S.A., Kukulski F., Lecka J., Robson S.C., Fernandes M.J., Sevigny J.
Biochemistry 43:5511-5519(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Liver.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY364442 mRNA. Translation: AAQ84519.1.
AL732585 Genomic DNA. Translation: CAM17997.1.
AL732585 Genomic DNA. Translation: CAM17998.1.
BC031143 mRNA. Translation: AAH31143.2.
CCDSCCDS15748.1. [Q8K0L2-1]
RefSeqNP_082369.1. NM_028093.1. [Q8K0L2-1]
XP_006498425.1. XM_006498362.1. [Q8K0L2-1]
UniGeneMm.33403.

3D structure databases

ProteinModelPortalQ8K0L2.
SMRQ8K0L2. Positions 42-459.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K0L2.

Proteomic databases

PRIDEQ8K0L2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813. [Q8K0L2-1]
ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813. [Q8K0L2-2]
ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813. [Q8K0L2-1]
GeneID72090.
KEGGmmu:72090.
UCSCuc008iqf.1. mouse. [Q8K0L2-1]

Organism-specific databases

CTD377841.
MGIMGI:1919340. Entpd8.

Phylogenomic databases

eggNOGCOG5371.
GeneTreeENSGT00550000074435.
HOGENOMHOG000059572.
HOVERGENHBG018982.
InParanoidQ8K0L2.
KOK01510.
OMAVDFWGAE.
OrthoDBEOG754HPX.
PhylomeDBQ8K0L2.
TreeFamTF332859.

Enzyme and pathway databases

SABIO-RKQ8K0L2.

Gene expression databases

BgeeQ8K0L2.
CleanExMM_ENTPD8.
GenevestigatorQ8K0L2.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio335412.
PROQ8K0L2.
SOURCESearch...

Entry information

Entry nameENTP8_MOUSE
AccessionPrimary (citable) accession number: Q8K0L2
Secondary accession number(s): A2AJ99, Q6UQ22
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: July 9, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot