Reviewed, UniProtKB/Swiss-Prot Q8K0L2 (ENTP8_MOUSE)

Names and origin

Protein names

Recommended name:
    Ectonucleoside triphosphate diphosphohydrolase 8
      Short name=E-NTPDase 8
      Short name=NTPDase 8
      Short name=NTPDase8
    EC=3.6.1.5

Gene names

Name:

Entpd8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolyzis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. Ref.1
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate. Ref.1
Cofactor	Calcium. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed in liver, jejunum and kidney. Ref.1
Domain	The transmembranous domains are involved in regulation of enzyme activity By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.
Biophysicochemical properties	Kinetic parameters: KM=13 µM for ATP KM=41 µM for ADP KM=47 µM for UTP KM=171 µM for UDP pH dependence: Optimum pH is 5.5-8.0.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	nucleoside diphosphate biosynthetic process Ref.1 Inferred from direct assay. Source: MGI nucleoside monophosphate biosynthetic process Ref.1 Inferred from direct assay. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Ref.1 Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Ref.1 Inferred from direct assay. Source: MGI nucleoside-triphosphatase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K0L2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K0L2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     263-265: Missing.
     353-389: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Ectonucleoside triphosphate diphosphohydrolase 8		PRO_0000306883
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Potential
	Topological domain	30 – 473	444	Extracellular Potential
	Transmembrane	474 – 494	21	Potential
	Topological domain	495 – 497	3	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	263 – 265	3	Missing in isoform 2.		VSP_028560
	Alternative sequence	353 – 389	37	Missing in isoform 2.		VSP_028561
Experimental info
	Sequence conflict	437	1	D → N in AAH31143. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 2, 2007. Version 3. Checksum: 00DE822B6EEB1BDF	FASTA	497	54,650
	10 20 30 40 50 60 MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG SSHTSLFVYQ 70 80 90 100 110 120 WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK SCLEEALALI PQAQHPETPT 130 140 150 160 170 180 FLGSTAGMRL LSQKNSSQAR DILAAVSQTL SKSPVDFWGA KILAGQDEGA FGWITINYVL 190 200 210 220 230 240 GMLLKYSSGQ WILPEEGMLV GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT 250 260 270 280 290 300 HSYLCFGRDQ ILNRLLAKLA QDRLSSQVAP VRHPCYHSGY QAILPLSSLY DSPCIHTTDS 310 320 330 340 350 360 LNHTQNLTVE GTGDPGNCVV ALRSLFNFSS CKGQKDCAFN GIYQPPVHGQ FYAFSNFYYT 370 380 390 400 410 420 FHFLNLTSRQ SLNTVNDTVW KFCQKPWKLV EVSYPGQERW LRDYCASGLY ILVLLLEGYK 430 440 450 460 470 480 FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL TGMIPAEAPT HWRAQSYSIW TAGVVFAVLT 490 LVAILGAAAI QIFWTQD
	Isoform 2. Checksum: 9A5BBDF66294C1CB	FASTA	457	49,766
	10 20 30 40 50 60 MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG SSHTSLFVYQ 70 80 90 100 110 120 WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK SCLEEALALI PQAQHPETPT 130 140 150 160 170 180 FLGSTAGMRL LSQKNSSQAR DILAAVSQTL SKSPVDFWGA KILAGQDEGA FGWITINYVL 190 200 210 220 230 240 GMLLKYSSGQ WILPEEGMLV GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT 250 260 270 280 290 300 HSYLCFGRDQ ILNRLLAKLA QDSQVAPVRH PCYHSGYQAI LPLSSLYDSP CIHTTDSLNH 310 320 330 340 350 360 TQNLTVEGTG DPGNCVVALR SLFNFSSCKG QKDCAFNGIY QPPVHGQFYV EVSYPGQERW 370 380 390 400 410 420 LRDYCASGLY ILVLLLEGYK FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL TGMIPAEAPT 430 440 450 HWRAQSYSIW TAGVVFAVLT LVAILGAAAI QIFWTQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8." Bigonnesse F., Levesque S.A., Kukulski F., Lecka J., Robson S.C., Fernandes M.J., Sevigny J. Biochemistry 43:5511-5519(2004) [PubMed: 15122917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Liver.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY364442 mRNA. Translation: AAQ84519.1. AL732585 Genomic DNA. Translation: CAM17997.1. AL732585 Genomic DNA. Translation: CAM17998.1. BC031143 mRNA. Translation: AAH31143.2.
IPI	IPI00420867. IPI00756255.
RefSeq	NP_082369.1.
UniGene	Mm.33403
3D structure databases
SMR	Q8K0L2. Positions 40-462.
ModBase	Search...
Proteomic databases
PRIDE	Q8K0L2.
Genome annotation databases
Ensembl	ENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813; Mus musculus. [Genome view] ENSMUST00000114378; ENSMUSP00000110019; ENSMUSG00000036813; Mus musculus. [Genome view] ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813; Mus musculus. [Genome view]
GeneID	72090.
KEGG	mmu:72090.
Organism-specific databases
CTD	72090.
MGI	MGI:1919340. Entpd8.
Phylogenomic databases
eggNOG	roNOG14650.
HOGENOM	HBG402995.
HOVERGEN	Q8K0L2.
InParanoid	Q8K0L2.
OMA	SYLCFGR.
OrthoDB	EOG9SJ806.
PhylomeDB	Q8K0L2.
Enzyme and pathway databases
BRENDA	3.6.1.5. 244.
Gene expression databases
ArrayExpress	Q8K0L2.
Bgee	Q8K0L2.
CleanEx	MM_ENTPD8.
Genevestigator	Q8K0L2.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	335412.
SOURCE	Search...

Entry information

Entry name

ENTP8_MOUSE

Accession

Primary (citable) accession number: Q8K0L2
Secondary accession number(s): A2AJ99, Q6UQ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	February 9, 2010
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)