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Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene

Entpd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.1 Publication

Cofactori

Ca2+1 Publication, Mg2+1 PublicationNote: Ca(2+) or Mg(2+). Has lower efficiency with Mg2+.1 Publication

Kineticsi

  1. KM=13 µM for ATP1 Publication
  2. KM=41 µM for ADP1 Publication
  3. KM=47 µM for UTP1 Publication
  4. KM=171 µM for UDP1 Publication

pH dependencei

Optimum pH is 5.5-8.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton acceptorBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleoside-diphosphatase activity Source: MGI
  4. nucleoside-triphosphatase activity Source: MGI

GO - Biological processi

  1. nucleoside diphosphate biosynthetic process Source: MGI
  2. nucleoside monophosphate biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.1.5. 3474.
SABIO-RKQ8K0L2.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
Short name:
E-NTPDase 8
Short name:
NTPDase 8
Short name:
NTPDase8
Gene namesi
Name:Entpd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919340. Entpd8.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 473444ExtracellularSequence AnalysisAdd
BLAST
Transmembranei474 – 49421HelicalSequence AnalysisAdd
BLAST
Topological domaini495 – 4973CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: MGI
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi78 ↔ 102By similarity
Disulfide bondi245 ↔ 294By similarity
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi331 ↔ 337By similarity
Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi383 ↔ 405By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8K0L2.

PTM databases

PhosphoSiteiQ8K0L2.

Expressioni

Tissue specificityi

Expressed in liver, jejunum and kidney.1 Publication

Gene expression databases

BgeeiQ8K0L2.
CleanExiMM_ENTPD8.
GenevestigatoriQ8K0L2.

Structurei

3D structure databases

ProteinModelPortaliQ8K0L2.
SMRiQ8K0L2. Positions 42-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The transmembranous domains are involved in regulation of enzyme activity.By similarity

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
GeneTreeiENSGT00550000074435.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiQ8K0L2.
KOiK01510.
OMAiVDFWGAE.
OrthoDBiEOG754HPX.
PhylomeDBiQ8K0L2.
TreeFamiTF332859.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K0L2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG
60 70 80 90 100
SSHTSLFVYQ WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK
110 120 130 140 150
SCLEEALALI PQAQHPETPT FLGSTAGMRL LSQKNSSQAR DILAAVSQTL
160 170 180 190 200
SKSPVDFWGA KILAGQDEGA FGWITINYVL GMLLKYSSGQ WILPEEGMLV
210 220 230 240 250
GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT HSYLCFGRDQ
260 270 280 290 300
ILNRLLAKLA QDRLSSQVAP VRHPCYHSGY QAILPLSSLY DSPCIHTTDS
310 320 330 340 350
LNHTQNLTVE GTGDPGNCVV ALRSLFNFSS CKGQKDCAFN GIYQPPVHGQ
360 370 380 390 400
FYAFSNFYYT FHFLNLTSRQ SLNTVNDTVW KFCQKPWKLV EVSYPGQERW
410 420 430 440 450
LRDYCASGLY ILVLLLEGYK FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL
460 470 480 490
TGMIPAEAPT HWRAQSYSIW TAGVVFAVLT LVAILGAAAI QIFWTQD
Length:497
Mass (Da):54,650
Last modified:October 1, 2007 - v3
Checksum:i00DE822B6EEB1BDF
GO
Isoform 2 (identifier: Q8K0L2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-265: Missing.
     353-389: Missing.

Show »
Length:457
Mass (Da):49,766
Checksum:i9A5BBDF66294C1CB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4371D → N in AAH31143 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei263 – 2653Missing in isoform 2. CuratedVSP_028560
Alternative sequencei353 – 38937Missing in isoform 2. CuratedVSP_028561Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY364442 mRNA. Translation: AAQ84519.1.
AL732585 Genomic DNA. Translation: CAM17997.1.
AL732585 Genomic DNA. Translation: CAM17998.1.
BC031143 mRNA. Translation: AAH31143.2.
CCDSiCCDS15748.1. [Q8K0L2-1]
RefSeqiNP_082369.1. NM_028093.1. [Q8K0L2-1]
XP_006498425.1. XM_006498362.2. [Q8K0L2-1]
UniGeneiMm.33403.

Genome annotation databases

EnsembliENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813. [Q8K0L2-1]
ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813. [Q8K0L2-2]
ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813. [Q8K0L2-1]
GeneIDi72090.
KEGGimmu:72090.
UCSCiuc008iqf.1. mouse. [Q8K0L2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY364442 mRNA. Translation: AAQ84519.1.
AL732585 Genomic DNA. Translation: CAM17997.1.
AL732585 Genomic DNA. Translation: CAM17998.1.
BC031143 mRNA. Translation: AAH31143.2.
CCDSiCCDS15748.1. [Q8K0L2-1]
RefSeqiNP_082369.1. NM_028093.1. [Q8K0L2-1]
XP_006498425.1. XM_006498362.2. [Q8K0L2-1]
UniGeneiMm.33403.

3D structure databases

ProteinModelPortaliQ8K0L2.
SMRiQ8K0L2. Positions 42-459.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8K0L2.

Proteomic databases

PRIDEiQ8K0L2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813. [Q8K0L2-1]
ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813. [Q8K0L2-2]
ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813. [Q8K0L2-1]
GeneIDi72090.
KEGGimmu:72090.
UCSCiuc008iqf.1. mouse. [Q8K0L2-1]

Organism-specific databases

CTDi377841.
MGIiMGI:1919340. Entpd8.

Phylogenomic databases

eggNOGiCOG5371.
GeneTreeiENSGT00550000074435.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiQ8K0L2.
KOiK01510.
OMAiVDFWGAE.
OrthoDBiEOG754HPX.
PhylomeDBiQ8K0L2.
TreeFamiTF332859.

Enzyme and pathway databases

BRENDAi3.6.1.5. 3474.
SABIO-RKQ8K0L2.

Miscellaneous databases

NextBioi335412.
PROiQ8K0L2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K0L2.
CleanExiMM_ENTPD8.
GenevestigatoriQ8K0L2.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8."
    Bigonnesse F., Levesque S.A., Kukulski F., Lecka J., Robson S.C., Fernandes M.J., Sevigny J.
    Biochemistry 43:5511-5519(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Kidney.

Entry informationi

Entry nameiENTP8_MOUSE
AccessioniPrimary (citable) accession number: Q8K0L2
Secondary accession number(s): A2AJ99, Q6UQ22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2007
Last sequence update: October 1, 2007
Last modified: March 31, 2015
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.