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Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene

Entpd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.1 Publication

Cofactori

Ca2+1 Publication, Mg2+1 PublicationNote: Ca(2+) or Mg(2+). Has lower efficiency with Mg2+.1 Publication

Kineticsi

  1. KM=13 µM for ATP1 Publication
  2. KM=41 µM for ADP1 Publication
  3. KM=47 µM for UTP1 Publication
  4. KM=171 µM for UDP1 Publication

    pH dependencei

    Optimum pH is 5.5-8.0.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681Proton acceptorBy similarity

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB-KW
    • nucleoside-diphosphatase activity Source: MGI
    • nucleoside-triphosphatase activity Source: MGI

    GO - Biological processi

    • nucleoside diphosphate biosynthetic process Source: MGI
    • nucleoside monophosphate biosynthetic process Source: MGI
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi3.6.1.5. 3474.
    SABIO-RKQ8K0L2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
    Short name:
    E-NTPDase 8
    Short name:
    NTPDase 8
    Short name:
    NTPDase8
    Gene namesi
    Name:Entpd8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 2

    Organism-specific databases

    MGIiMGI:1919340. Entpd8.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Transmembranei9 – 2921HelicalSequence AnalysisAdd
    BLAST
    Topological domaini30 – 473444ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei474 – 49421HelicalSequence AnalysisAdd
    BLAST
    Topological domaini495 – 4973CytoplasmicSequence Analysis

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • membrane Source: MGI
    • plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 497497Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306883Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi78 ↔ 102By similarity
    Disulfide bondi245 ↔ 294By similarity
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi331 ↔ 337By similarity
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi383 ↔ 405By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8K0L2.

    PTM databases

    PhosphoSiteiQ8K0L2.

    Expressioni

    Tissue specificityi

    Expressed in liver, jejunum and kidney.1 Publication

    Gene expression databases

    BgeeiQ8K0L2.
    CleanExiMM_ENTPD8.
    GenevisibleiQ8K0L2. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000040628.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K0L2.
    SMRiQ8K0L2. Positions 42-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The transmembranous domains are involved in regulation of enzyme activity.By similarity

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    GeneTreeiENSGT00550000074435.
    HOGENOMiHOG000059572.
    HOVERGENiHBG018982.
    InParanoidiQ8K0L2.
    KOiK01510.
    OMAiVDFWGAE.
    OrthoDBiEOG754HPX.
    PhylomeDBiQ8K0L2.
    TreeFamiTF332859.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8K0L2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG
    60 70 80 90 100
    SSHTSLFVYQ WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK
    110 120 130 140 150
    SCLEEALALI PQAQHPETPT FLGSTAGMRL LSQKNSSQAR DILAAVSQTL
    160 170 180 190 200
    SKSPVDFWGA KILAGQDEGA FGWITINYVL GMLLKYSSGQ WILPEEGMLV
    210 220 230 240 250
    GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT HSYLCFGRDQ
    260 270 280 290 300
    ILNRLLAKLA QDRLSSQVAP VRHPCYHSGY QAILPLSSLY DSPCIHTTDS
    310 320 330 340 350
    LNHTQNLTVE GTGDPGNCVV ALRSLFNFSS CKGQKDCAFN GIYQPPVHGQ
    360 370 380 390 400
    FYAFSNFYYT FHFLNLTSRQ SLNTVNDTVW KFCQKPWKLV EVSYPGQERW
    410 420 430 440 450
    LRDYCASGLY ILVLLLEGYK FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL
    460 470 480 490
    TGMIPAEAPT HWRAQSYSIW TAGVVFAVLT LVAILGAAAI QIFWTQD
    Length:497
    Mass (Da):54,650
    Last modified:October 2, 2007 - v3
    Checksum:i00DE822B6EEB1BDF
    GO
    Isoform 2 (identifier: Q8K0L2-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         263-265: Missing.
         353-389: Missing.

    Show »
    Length:457
    Mass (Da):49,766
    Checksum:i9A5BBDF66294C1CB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti437 – 4371D → N in AAH31143 (PubMed:15489334).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei263 – 2653Missing in isoform 2. CuratedVSP_028560
    Alternative sequencei353 – 38937Missing in isoform 2. CuratedVSP_028561Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY364442 mRNA. Translation: AAQ84519.1.
    AL732585 Genomic DNA. Translation: CAM17997.1.
    AL732585 Genomic DNA. Translation: CAM17998.1.
    BC031143 mRNA. Translation: AAH31143.2.
    CCDSiCCDS15748.1. [Q8K0L2-1]
    RefSeqiNP_082369.1. NM_028093.1. [Q8K0L2-1]
    XP_006498425.1. XM_006498362.2. [Q8K0L2-1]
    UniGeneiMm.33403.

    Genome annotation databases

    EnsembliENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813. [Q8K0L2-1]
    ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813. [Q8K0L2-2]
    ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813. [Q8K0L2-1]
    GeneIDi72090.
    KEGGimmu:72090.
    UCSCiuc008iqf.1. mouse. [Q8K0L2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY364442 mRNA. Translation: AAQ84519.1.
    AL732585 Genomic DNA. Translation: CAM17997.1.
    AL732585 Genomic DNA. Translation: CAM17998.1.
    BC031143 mRNA. Translation: AAH31143.2.
    CCDSiCCDS15748.1. [Q8K0L2-1]
    RefSeqiNP_082369.1. NM_028093.1. [Q8K0L2-1]
    XP_006498425.1. XM_006498362.2. [Q8K0L2-1]
    UniGeneiMm.33403.

    3D structure databases

    ProteinModelPortaliQ8K0L2.
    SMRiQ8K0L2. Positions 42-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000040628.

    PTM databases

    PhosphoSiteiQ8K0L2.

    Proteomic databases

    PRIDEiQ8K0L2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813. [Q8K0L2-1]
    ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813. [Q8K0L2-2]
    ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813. [Q8K0L2-1]
    GeneIDi72090.
    KEGGimmu:72090.
    UCSCiuc008iqf.1. mouse. [Q8K0L2-1]

    Organism-specific databases

    CTDi377841.
    MGIiMGI:1919340. Entpd8.

    Phylogenomic databases

    eggNOGiCOG5371.
    GeneTreeiENSGT00550000074435.
    HOGENOMiHOG000059572.
    HOVERGENiHBG018982.
    InParanoidiQ8K0L2.
    KOiK01510.
    OMAiVDFWGAE.
    OrthoDBiEOG754HPX.
    PhylomeDBiQ8K0L2.
    TreeFamiTF332859.

    Enzyme and pathway databases

    BRENDAi3.6.1.5. 3474.
    SABIO-RKQ8K0L2.

    Miscellaneous databases

    NextBioi335412.
    PROiQ8K0L2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ8K0L2.
    CleanExiMM_ENTPD8.
    GenevisibleiQ8K0L2. MM.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8."
      Bigonnesse F., Levesque S.A., Kukulski F., Lecka J., Robson S.C., Fernandes M.J., Sevigny J.
      Biochemistry 43:5511-5519(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Kidney.

    Entry informationi

    Entry nameiENTP8_MOUSE
    AccessioniPrimary (citable) accession number: Q8K0L2
    Secondary accession number(s): A2AJ99, Q6UQ22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: June 24, 2015
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.