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Protein

Ankyrin repeat and SOCS box protein 2

Gene

Asb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • mitophagy in response to mitochondrial depolarization Source: MGI
  • myoblast differentiation Source: MGI
  • positive regulation of defense response to virus by host Source: MGI
  • protein polyubiquitination Source: MGI
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: MGI
  • skeletal muscle cell differentiation Source: MGI
  • xenophagy Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SOCS box protein 2
Short name:
ASB-2
Gene namesi
Name:Asb2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1929743. Asb2.

Subcellular locationi

GO - Cellular componenti

  • Cul5-RING ubiquitin ligase complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 634634Ankyrin repeat and SOCS box protein 2PRO_0000233303Add
BLAST

Proteomic databases

PaxDbiQ8K0L0.
PRIDEiQ8K0L0.

PTM databases

iPTMnetiQ8K0L0.
PhosphoSiteiQ8K0L0.

Expressioni

Tissue specificityi

Highest expression in muscle, heart and spleen.1 Publication

Gene expression databases

BgeeiQ8K0L0.
CleanExiMM_ASB2.
GenevisibleiQ8K0L0. MM.

Interactioni

Subunit structurei

Component of a probable ECS E3 ubiquitin-protein ligase complex which contains CUL5, either RBX1 or RNF7/RBX2, Elongin BC complex (TCEB1 and TCEB2) and ASB2.By similarity

Protein-protein interaction databases

BioGridi211145. 2 interactions.
IntActiQ8K0L0. 1 interaction.
STRINGi10090.ENSMUSP00000021617.

Structurei

3D structure databases

ProteinModelPortaliQ8K0L0.
SMRiQ8K0L0. Positions 105-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 4520UIMPROSITE-ProRule annotationAdd
BLAST
Repeati104 – 13330ANK 1Sequence analysisAdd
BLAST
Repeati137 – 16731ANK 2Sequence analysisAdd
BLAST
Repeati171 – 20030ANK 3Sequence analysisAdd
BLAST
Repeati204 – 23330ANK 4Sequence analysisAdd
BLAST
Repeati237 – 26630ANK 5Sequence analysisAdd
BLAST
Repeati270 – 29930ANK 6Sequence analysisAdd
BLAST
Repeati303 – 33230ANK 7Sequence analysisAdd
BLAST
Repeati336 – 36530ANK 8Sequence analysisAdd
BLAST
Repeati368 – 39730ANK 9Sequence analysisAdd
BLAST
Repeati410 – 43930ANK 10Sequence analysisAdd
BLAST
Repeati440 – 46930ANK 11Sequence analysisAdd
BLAST
Repeati476 – 50429ANK 12Sequence analysisAdd
BLAST
Domaini580 – 63455SOCS boxPROSITE-ProRule annotationAdd
BLAST

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.By similarity

Sequence similaritiesi

Belongs to the ankyrin SOCS box (ASB) family.Curated
Contains 12 ANK repeats.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00530000063050.
HOGENOMiHOG000034086.
HOVERGENiHBG104037.
InParanoidiQ8K0L0.
KOiK10324.
OMAiRPLAHLC.
OrthoDBiEOG77DJ5P.
PhylomeDBiQ8K0L0.
TreeFamiTF315127.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF13606. Ank_3. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 11 hits.
SM00253. SOCS. 1 hit.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 8 hits.
PS50225. SOCS. 1 hit.
PS50330. UIM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8K0L0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATEISTRGR QRAIGHEEYS LYSSLSEEEL LQMAIEQSLA DKTRGPTPAE
60 70 80 90 100
ASASSQTNHQ PGHFHPWTRS PSSPENPPAR APLGLFQGVM QKYSSNLFKT
110 120 130 140 150
SQMAAMDPVL KAIKEGDEEA LKIMIQDGKN LAEPNKEGWL PLHEAAYYGQ
160 170 180 190 200
LGCLKVLQQA YPGTIDQRTL QEETALYLAT CREHLDCLLS LLQAGAEPDI
210 220 230 240 250
SNKSRETPLY KACERKNAEA VRILVRYNAD ANHRCNRGWT ALHESVSRND
260 270 280 290 300
LEVMEILVSG GAKVEAKNVY SITPLFVAAQ SGQLEALRFL AKHGADINTQ
310 320 330 340 350
ASDSASALYE ASKNEHEDVV EFLLSQGADA NKANKDGLLP LHVASKKGNY
360 370 380 390 400
RIVQMLLPVT SRTRVRRSGI SPLHLAAERN HDAVLEALLA ARFDVNAPLA
410 420 430 440 450
PERARLYEDR RSSALYFAVV NNNVYATELL LLAGADPNRD VISPLLVAIR
460 470 480 490 500
HGCLRTMQLL LDHGANIDAY IATHPTAFPA TIMFAMKCLS LLKFLMDLGC
510 520 530 540 550
DGEPCFSCLY GNGPHPPAPR PGRFHDAPVD DKAPSVVQFC EFLSAPEVSR
560 570 580 590 600
WAGPIIDVLL DYVGNVQLCS RLKEHIDSFE DWAVIKEKAE PPRPLAHLCR
610 620 630
LRVRKAIGKY RIKLLDTLPL PGRLIRYLKY ENTQ
Length:634
Mass (Da):70,221
Last modified:October 1, 2002 - v1
Checksum:iE320CEBD39891D41
GO
Isoform 21 Publication (identifier: Q8K0L0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     49-68: AEASASSQTNHQPGHFHPWT → MTRFSYAEYFALFHSGSAPS

Show »
Length:586
Mass (Da):64,937
Checksum:i79D011C89B571DEC
GO

Sequence cautioni

The sequence AAD38809.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 732SS → LF in AAD38809 (PubMed:11111040).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4848Missing in isoform 2. 1 PublicationVSP_052025Add
BLAST
Alternative sequencei49 – 6820AEASA…FHPWT → MTRFSYAEYFALFHSGSAPS in isoform 2. 1 PublicationVSP_052026Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155353 mRNA. Translation: AAD38809.2. Different initiation.
BC031161 mRNA. Translation: AAH31161.1.
AK003566 mRNA. Translation: BAB22862.1.
CCDSiCCDS26128.1. [Q8K0L0-1]
RefSeqiNP_075536.1. NM_023049.1. [Q8K0L0-1]
UniGeneiMm.27159.
Mm.321278.

Genome annotation databases

EnsembliENSMUST00000021617; ENSMUSP00000021617; ENSMUSG00000021200. [Q8K0L0-1]
ENSMUST00000149431; ENSMUSP00000117595; ENSMUSG00000021200. [Q8K0L0-2]
GeneIDi65256.
KEGGimmu:65256.
UCSCiuc007ovc.1. mouse. [Q8K0L0-1]
uc007ovd.1. mouse. [Q8K0L0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155353 mRNA. Translation: AAD38809.2. Different initiation.
BC031161 mRNA. Translation: AAH31161.1.
AK003566 mRNA. Translation: BAB22862.1.
CCDSiCCDS26128.1. [Q8K0L0-1]
RefSeqiNP_075536.1. NM_023049.1. [Q8K0L0-1]
UniGeneiMm.27159.
Mm.321278.

3D structure databases

ProteinModelPortaliQ8K0L0.
SMRiQ8K0L0. Positions 105-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211145. 2 interactions.
IntActiQ8K0L0. 1 interaction.
STRINGi10090.ENSMUSP00000021617.

PTM databases

iPTMnetiQ8K0L0.
PhosphoSiteiQ8K0L0.

Proteomic databases

PaxDbiQ8K0L0.
PRIDEiQ8K0L0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021617; ENSMUSP00000021617; ENSMUSG00000021200. [Q8K0L0-1]
ENSMUST00000149431; ENSMUSP00000117595; ENSMUSG00000021200. [Q8K0L0-2]
GeneIDi65256.
KEGGimmu:65256.
UCSCiuc007ovc.1. mouse. [Q8K0L0-1]
uc007ovd.1. mouse. [Q8K0L0-2]

Organism-specific databases

CTDi51676.
MGIiMGI:1929743. Asb2.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00530000063050.
HOGENOMiHOG000034086.
HOVERGENiHBG104037.
InParanoidiQ8K0L0.
KOiK10324.
OMAiRPLAHLC.
OrthoDBiEOG77DJ5P.
PhylomeDBiQ8K0L0.
TreeFamiTF315127.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi320348.
PROiQ8K0L0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K0L0.
CleanExiMM_ASB2.
GenevisibleiQ8K0L0. MM.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF13606. Ank_3. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 11 hits.
SM00253. SOCS. 1 hit.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 8 hits.
PS50225. SOCS. 1 hit.
PS50330. UIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4."
    Kile B.T., Viney E.M., Willson T.A., Brodnicki T.C., Cancilla M.R., Herlihy A.S., Croker B.A., Baca M., Nicola N.A., Hilton D.J., Alexander W.S.
    Gene 258:31-41(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Strain: C57BL/6JImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/NImported.
    Tissue: ColonImported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 472-634.
    Strain: C57BL/6JImported.
    Tissue: Embryo1 Publication.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart.

Entry informationi

Entry nameiASB2_MOUSE
AccessioniPrimary (citable) accession number: Q8K0L0
Secondary accession number(s): Q9CTH4, Q9WV73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.