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Q8K0H1 (S47A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug and toxin extrusion protein 1

Short name=MATE-1
Short name=mMATE-1
Alternative name(s):
Solute carrier family 47 member 1
Gene names
Name:Slc47a1
Synonyms:Mate1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length567 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes By similarity. Ref.5 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Predominantly localized to the plasma membrane; at the brush border membranes of the proximal tubules (kidney) and at the bile caniculi (liver). Ref.4 Ref.5 Ref.6

Tissue specificity

Predominantly expressed in kidney and liver. Also expressed in various cells, including brain glia-like cells and capillaries, pancreatic duct cells, urinary bladder epithelium, adrenal gland cortex, heart, stomach, small intestine, thyroid gland, testes, alpha cells of the islets of Langerhans, Leydig cells, and vitamin A-storing Ito cells. Expressed in heart, stomach, small intestine, bladder, thyroid gland, adrenal gland and testes (at protein level). Ref.4

Sequence similarities

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=0.41 mM for TEA Ref.5

Vmax=0.6 nmol/min/mg enzyme toward TEA

pH dependence:

Optimum pH is 8.0-8.5. Active from pH 6 to 8.5.

Sequence caution

The sequence BC031436 differs from that shown. Reason: Frameshift at position 510.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K0H1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K0H1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.
     143-181: LFRQDPDVSR...TLQVKYLLNQ → MSDTSPQAGV...SGLAGLLERV
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 567567Multidrug and toxin extrusion protein 1
PRO_0000312846

Regions

Topological domain1 – 3737Cytoplasmic Potential
Transmembrane38 – 5821Helical; Potential
Topological domain59 – 7214Extracellular Potential
Transmembrane73 – 9321Helical; Potential
Topological domain94 – 12027Cytoplasmic Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15211Extracellular Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 18714Cytoplasmic Potential
Transmembrane188 – 20821Helical; Potential
Topological domain209 – 2168Extracellular Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 25720Cytoplasmic Potential
Transmembrane258 – 27720Helical; Potential
Topological domain278 – 29518Extracellular Potential
Transmembrane296 – 31621Helical; Potential
Topological domain317 – 33620Cytoplasmic Potential
Transmembrane337 – 35721Helical; Potential
Topological domain358 – 37013Extracellular Potential
Transmembrane371 – 39121Helical; Potential
Topological domain392 – 40817Cytoplasmic Potential
Transmembrane409 – 42921Helical; Potential
Topological domain430 – 4378Extracellular Potential
Transmembrane438 – 45821Helical; Potential
Topological domain459 – 54385Cytoplasmic Potential
Transmembrane544 – 56421Helical; Potential
Topological domain565 – 5673Extracellular Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence1 – 142142Missing in isoform 2.
VSP_029906
Alternative sequence143 – 18139LFRQD…YLLNQ → MSDTSPQAGVLSRARLLQLR RHSSQRPERSGLAGLLERV in isoform 2.
VSP_029907

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: EDAF1D3DB3BBD8F5

FASTA56761,642
        10         20         30         40         50         60 
MERTEESAPG PGGADAASER RGLRCLLLPG FLEELRALLV LAGPAFLAQL MMFLISFISS 

        70         80         90        100        110        120 
VFCGHLGKLE LDAVTLAIAV INVTGISVGH GLSSACDTLI SQTYGSQNLK HVGVILQRGT 

       130        140        150        160        170        180 
LILLLCCFPC WALFINTEQI LLLFRQDPDV SRLTQTYVMI FIPALPAAFL YTLQVKYLLN 

       190        200        210        220        230        240 
QGIVLPQIMT GIAANLVNAL ANYVFLYHLH LGVMGSALAN TISQFALAIF LFLYILWRRL 

       250        260        270        280        290        300 
HQATWGGWSW ECLQDWASFL RLAIPSMLML CIEWWAYEVG SFLSGILGMV ELGAQSITYE 

       310        320        330        340        350        360 
LAIIVYMIPS GFSVAANVRV GNALGAGNID QAKKSSAISL IVTELFAVTF CVLLLGCKDL 

       370        380        390        400        410        420 
VGYIFTTDRD IVALVAQVIP IYAVSHLFEG LACTCGGILR GTGNQKVGAI VNAIGYYVIG 

       430        440        450        460        470        480 
LPIGIALMFA AKLGVIGLWS GIIICTTCQT TCFLAFIARL NWKRACQQAQ VHANLKVNVA 

       490        500        510        520        530        540 
LNSAVSHEPA HPVCPESHGE IMMTDLEKKD ETQLDQPMNQ QQALPIRPKD SNKLSGKQLA 

       550        560 
LRRGLLLLGV VLVLVGGILV RVYIRIE 

« Hide

Isoform 2 [UniParc].

Checksum: BF6B65414F86C3DC
Show »

FASTA42546,259

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Liver and Tongue.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Kidney.
[4]"A human transporter protein that mediates the final excretion step for toxic organic cations."
Otsuka M., Matsumoto T., Morimoto R., Arioka S., Omote H., Moriyama Y.
Proc. Natl. Acad. Sci. U.S.A. 102:17923-17928(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations."
Hiasa M., Matsumoto T., Komatsu T., Moriyama Y.
Am. J. Physiol. 291:C678-C686(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]"Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter."
Hiasa M., Matsumoto T., Komatsu T., Omote H., Moriyama Y.
Am. J. Physiol. 293:C1437-C1444(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK004994 mRNA. Translation: BAB23729.1.
AK009038 mRNA. Translation: BAB26040.1.
AL669884 Genomic DNA. Translation: CAI25734.1.
BC031436 mRNA. No translation available.
CCDSCCDS24811.2. [Q8K0H1-1]
RefSeqNP_080459.2. NM_026183.5. [Q8K0H1-1]
XP_006534055.1. XM_006533992.1. [Q8K0H1-2]
UniGeneMm.100741.

3D structure databases

ProteinModelPortalQ8K0H1.
SMRQ8K0H1. Positions 31-476.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

PhosphoSiteQ8K0H1.

Proteomic databases

MaxQBQ8K0H1.
PaxDbQ8K0H1.
PRIDEQ8K0H1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000010267; ENSMUSP00000010267; ENSMUSG00000010122. [Q8K0H1-1]
GeneID67473.
KEGGmmu:67473.
UCSCuc007jhh.2. mouse. [Q8K0H1-2]
uc007jhi.2. mouse. [Q8K0H1-1]

Organism-specific databases

CTD55244.
MGIMGI:1914723. Slc47a1.

Phylogenomic databases

eggNOGCOG0534.
GeneTreeENSGT00390000015713.
HOGENOMHOG000060313.
HOVERGENHBG056043.
InParanoidQ8K0H1.
KOK03327.
OMAFPRYLVM.
OrthoDBEOG7RRF71.
PhylomeDBQ8K0H1.
TreeFamTF324441.

Gene expression databases

ArrayExpressQ8K0H1.
BgeeQ8K0H1.
CleanExMM_SLC47A1.
GenevestigatorQ8K0H1.

Family and domain databases

InterProIPR002528. MATE.
[Graphical view]
PfamPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsTIGR00797. matE. 1 hit.
ProtoNetSearch...

Other

NextBio324686.
PROQ8K0H1.
SOURCESearch...

Entry information

Entry nameS47A1_MOUSE
AccessionPrimary (citable) accession number: Q8K0H1
Secondary accession number(s): Q5SS45, Q9CQ64
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot