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Q8K0H1

- S47A1_MOUSE

UniProt

Q8K0H1 - S47A1_MOUSE

Protein

Multidrug and toxin extrusion protein 1

Gene

Slc47a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes By similarity.By similarity

    Kineticsi

    1. KM=0.41 mM for TEA1 Publication

    Vmax=0.6 nmol/min/mg enzyme toward TEA1 Publication

    pH dependencei

    Optimum pH is 8.0-8.5. Active from pH 6 to 8.5.1 Publication

    GO - Molecular functioni

    1. drug transmembrane transporter activity Source: Ensembl
    2. monovalent cation:proton antiporter activity Source: Ensembl

    GO - Biological processi

    1. organic cation transport Source: Ensembl

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

    Protein family/group databases

    TCDBi2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multidrug and toxin extrusion protein 1
    Short name:
    MATE-1
    Short name:
    mMATE-1
    Alternative name(s):
    Solute carrier family 47 member 1
    Gene namesi
    Name:Slc47a1
    Synonyms:Mate1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1914723. Slc47a1.

    Subcellular locationi

    Cell membrane 3 Publications; Multi-pass membrane protein 3 Publications
    Note: Predominantly localized to the plasma membrane; at the brush border membranes of the proximal tubules (kidney) and at the bile caniculi (liver).

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane-bounded vesicle Source: Ensembl
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 567567Multidrug and toxin extrusion protein 1PRO_0000312846Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8K0H1.
    PaxDbiQ8K0H1.
    PRIDEiQ8K0H1.

    PTM databases

    PhosphoSiteiQ8K0H1.

    Expressioni

    Tissue specificityi

    Predominantly expressed in kidney and liver. Also expressed in various cells, including brain glia-like cells and capillaries, pancreatic duct cells, urinary bladder epithelium, adrenal gland cortex, heart, stomach, small intestine, thyroid gland, testes, alpha cells of the islets of Langerhans, Leydig cells, and vitamin A-storing Ito cells. Expressed in heart, stomach, small intestine, bladder, thyroid gland, adrenal gland and testes (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ8K0H1.
    BgeeiQ8K0H1.
    CleanExiMM_SLC47A1.
    GenevestigatoriQ8K0H1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K0H1.
    SMRiQ8K0H1. Positions 31-476.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3737CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini59 – 7214ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini94 – 12027CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini142 – 15211ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini174 – 18714CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini209 – 2168ExtracellularSequence Analysis
    Topological domaini238 – 25720CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini278 – 29518ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini317 – 33620CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini358 – 37013ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini392 – 40817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini430 – 4378ExtracellularSequence Analysis
    Topological domaini459 – 54385CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini565 – 5673ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei38 – 5821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei73 – 9321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei153 – 17321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei188 – 20821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei258 – 27720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei296 – 31621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei337 – 35721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei371 – 39121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei409 – 42921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei438 – 45821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei544 – 56421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0534.
    GeneTreeiENSGT00390000015713.
    HOGENOMiHOG000060313.
    HOVERGENiHBG056043.
    InParanoidiQ8K0H1.
    KOiK03327.
    OMAiFPRYLVM.
    OrthoDBiEOG7RRF71.
    PhylomeDBiQ8K0H1.
    TreeFamiTF324441.

    Family and domain databases

    InterProiIPR002528. MATE.
    [Graphical view]
    PfamiPF01554. MatE. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00797. matE. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K0H1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERTEESAPG PGGADAASER RGLRCLLLPG FLEELRALLV LAGPAFLAQL    50
    MMFLISFISS VFCGHLGKLE LDAVTLAIAV INVTGISVGH GLSSACDTLI 100
    SQTYGSQNLK HVGVILQRGT LILLLCCFPC WALFINTEQI LLLFRQDPDV 150
    SRLTQTYVMI FIPALPAAFL YTLQVKYLLN QGIVLPQIMT GIAANLVNAL 200
    ANYVFLYHLH LGVMGSALAN TISQFALAIF LFLYILWRRL HQATWGGWSW 250
    ECLQDWASFL RLAIPSMLML CIEWWAYEVG SFLSGILGMV ELGAQSITYE 300
    LAIIVYMIPS GFSVAANVRV GNALGAGNID QAKKSSAISL IVTELFAVTF 350
    CVLLLGCKDL VGYIFTTDRD IVALVAQVIP IYAVSHLFEG LACTCGGILR 400
    GTGNQKVGAI VNAIGYYVIG LPIGIALMFA AKLGVIGLWS GIIICTTCQT 450
    TCFLAFIARL NWKRACQQAQ VHANLKVNVA LNSAVSHEPA HPVCPESHGE 500
    IMMTDLEKKD ETQLDQPMNQ QQALPIRPKD SNKLSGKQLA LRRGLLLLGV 550
    VLVLVGGILV RVYIRIE 567
    Length:567
    Mass (Da):61,642
    Last modified:December 4, 2007 - v2
    Checksum:iEDAF1D3DB3BBD8F5
    GO
    Isoform 2 (identifier: Q8K0H1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-142: Missing.
         143-181: LFRQDPDVSR...TLQVKYLLNQ → MSDTSPQAGV...SGLAGLLERV

    Note: No experimental confirmation available.

    Show »
    Length:425
    Mass (Da):46,259
    Checksum:iBF6B65414F86C3DC
    GO

    Sequence cautioni

    The sequence BC031436 differs from that shown. Reason: Frameshift at position 510.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 142142Missing in isoform 2. 1 PublicationVSP_029906Add
    BLAST
    Alternative sequencei143 – 18139LFRQD…YLLNQ → MSDTSPQAGVLSRARLLQLR RHSSQRPERSGLAGLLERV in isoform 2. 1 PublicationVSP_029907Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004994 mRNA. Translation: BAB23729.1.
    AK009038 mRNA. Translation: BAB26040.1.
    AL669884 Genomic DNA. Translation: CAI25734.1.
    BC031436 mRNA. No translation available.
    CCDSiCCDS24811.2. [Q8K0H1-1]
    RefSeqiNP_080459.2. NM_026183.5. [Q8K0H1-1]
    XP_006534055.1. XM_006533992.1. [Q8K0H1-2]
    UniGeneiMm.100741.

    Genome annotation databases

    EnsembliENSMUST00000010267; ENSMUSP00000010267; ENSMUSG00000010122. [Q8K0H1-1]
    GeneIDi67473.
    KEGGimmu:67473.
    UCSCiuc007jhh.2. mouse. [Q8K0H1-2]
    uc007jhi.2. mouse. [Q8K0H1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004994 mRNA. Translation: BAB23729.1 .
    AK009038 mRNA. Translation: BAB26040.1 .
    AL669884 Genomic DNA. Translation: CAI25734.1 .
    BC031436 mRNA. No translation available.
    CCDSi CCDS24811.2. [Q8K0H1-1 ]
    RefSeqi NP_080459.2. NM_026183.5. [Q8K0H1-1 ]
    XP_006534055.1. XM_006533992.1. [Q8K0H1-2 ]
    UniGenei Mm.100741.

    3D structure databases

    ProteinModelPortali Q8K0H1.
    SMRi Q8K0H1. Positions 31-476.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

    PTM databases

    PhosphoSitei Q8K0H1.

    Proteomic databases

    MaxQBi Q8K0H1.
    PaxDbi Q8K0H1.
    PRIDEi Q8K0H1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010267 ; ENSMUSP00000010267 ; ENSMUSG00000010122 . [Q8K0H1-1 ]
    GeneIDi 67473.
    KEGGi mmu:67473.
    UCSCi uc007jhh.2. mouse. [Q8K0H1-2 ]
    uc007jhi.2. mouse. [Q8K0H1-1 ]

    Organism-specific databases

    CTDi 55244.
    MGIi MGI:1914723. Slc47a1.

    Phylogenomic databases

    eggNOGi COG0534.
    GeneTreei ENSGT00390000015713.
    HOGENOMi HOG000060313.
    HOVERGENi HBG056043.
    InParanoidi Q8K0H1.
    KOi K03327.
    OMAi FPRYLVM.
    OrthoDBi EOG7RRF71.
    PhylomeDBi Q8K0H1.
    TreeFami TF324441.

    Enzyme and pathway databases

    Reactomei REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

    Miscellaneous databases

    NextBioi 324686.
    PROi Q8K0H1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K0H1.
    Bgeei Q8K0H1.
    CleanExi MM_SLC47A1.
    Genevestigatori Q8K0H1.

    Family and domain databases

    InterProi IPR002528. MATE.
    [Graphical view ]
    Pfami PF01554. MatE. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00797. matE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Liver and Tongue.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Kidney.
    4. "A human transporter protein that mediates the final excretion step for toxic organic cations."
      Otsuka M., Matsumoto T., Morimoto R., Arioka S., Omote H., Moriyama Y.
      Proc. Natl. Acad. Sci. U.S.A. 102:17923-17928(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    5. "Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations."
      Hiasa M., Matsumoto T., Komatsu T., Moriyama Y.
      Am. J. Physiol. 291:C678-C686(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter."
      Hiasa M., Matsumoto T., Komatsu T., Omote H., Moriyama Y.
      Am. J. Physiol. 293:C1437-C1444(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiS47A1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K0H1
    Secondary accession number(s): Q5SS45, Q9CQ64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3