Q8K0H1 (S47A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug and toxin extrusion protein 1
Short name=MATE-1
Short name=mMATE-1

Alternative name(s):
Solute carrier family 47 member 1

Gene names

Name:	Slc47a1
Synonyms:	Mate1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes By similarity. Ref.5 Ref.6
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Predominantly localized to the plasma membrane; at the brush border membranes of the proximal tubules (kidney) and at the bile caniculi (liver). Ref.4 Ref.5 Ref.6
Tissue specificity	Predominantly expressed in kidney and liver. Also expressed in various cells, including brain glia-like cells and capillaries, pancreatic duct cells, urinary bladder epithelium, adrenal gland cortex, heart, stomach, small intestine, thyroid gland, testes, alpha cells of the islets of Langerhans, Leydig cells, and vitamin A-storing Ito cells. Expressed in heart, stomach, small intestine, bladder, thyroid gland, adrenal gland and testes (at protein level). Ref.4
Sequence similarities	Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. [View classification]
Biophysicochemical properties	Kinetic parameters: KM=0.41 mM for TEA Ref.5 Vmax=0.6 nmol/min/mg enzyme toward TEA pH dependence: Optimum pH is 8.0-8.5. Active from pH 6 to 8.5.
Sequence caution	The sequence BC031436 differs from that shown. Reason: Frameshift at position 510.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	organic cation transport Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane-bounded vesicle Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	drug transmembrane transporter activity Inferred from electronic annotation. Source: Compara monovalent cation:hydrogen antiporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K0H1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K0H1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.
     143-181: LFRQDPDVSR...TLQVKYLLNQ → MSDTSPQAGV...SGLAGLLERV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Multidrug and toxin extrusion protein 1		PRO_0000312846
Regions
	Topological domain	1 – 37	37	Cytoplasmic Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Topological domain	59 – 72	14	Extracellular Potential
	Transmembrane	73 – 93	21	Helical; Potential
	Topological domain	94 – 120	27	Cytoplasmic Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Topological domain	142 – 152	11	Extracellular Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Topological domain	174 – 187	14	Cytoplasmic Potential
	Transmembrane	188 – 208	21	Helical; Potential
	Topological domain	209 – 216	8	Extracellular Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Topological domain	238 – 257	20	Cytoplasmic Potential
	Transmembrane	258 – 277	20	Helical; Potential
	Topological domain	278 – 295	18	Extracellular Potential
	Transmembrane	296 – 316	21	Helical; Potential
	Topological domain	317 – 336	20	Cytoplasmic Potential
	Transmembrane	337 – 357	21	Helical; Potential
	Topological domain	358 – 370	13	Extracellular Potential
	Transmembrane	371 – 391	21	Helical; Potential
	Topological domain	392 – 408	17	Cytoplasmic Potential
	Transmembrane	409 – 429	21	Helical; Potential
	Topological domain	430 – 437	8	Extracellular Potential
	Transmembrane	438 – 458	21	Helical; Potential
	Topological domain	459 – 543	85	Cytoplasmic Potential
	Transmembrane	544 – 564	21	Helical; Potential
	Topological domain	565 – 567	3	Extracellular Potential
Natural variations
	Alternative sequence	1 – 142	142	Missing in isoform 2.		VSP_029906
	Alternative sequence	143 – 181	39	LFRQD…YLLNQ → MSDTSPQAGVLSRARLLQLR RHSSQRPERSGLAGLLERV in isoform 2.		VSP_029907

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: EDAF1D3DB3BBD8F5	FASTA	567	61,642
	10 20 30 40 50 60 MERTEESAPG PGGADAASER RGLRCLLLPG FLEELRALLV LAGPAFLAQL MMFLISFISS 70 80 90 100 110 120 VFCGHLGKLE LDAVTLAIAV INVTGISVGH GLSSACDTLI SQTYGSQNLK HVGVILQRGT 130 140 150 160 170 180 LILLLCCFPC WALFINTEQI LLLFRQDPDV SRLTQTYVMI FIPALPAAFL YTLQVKYLLN 190 200 210 220 230 240 QGIVLPQIMT GIAANLVNAL ANYVFLYHLH LGVMGSALAN TISQFALAIF LFLYILWRRL 250 260 270 280 290 300 HQATWGGWSW ECLQDWASFL RLAIPSMLML CIEWWAYEVG SFLSGILGMV ELGAQSITYE 310 320 330 340 350 360 LAIIVYMIPS GFSVAANVRV GNALGAGNID QAKKSSAISL IVTELFAVTF CVLLLGCKDL 370 380 390 400 410 420 VGYIFTTDRD IVALVAQVIP IYAVSHLFEG LACTCGGILR GTGNQKVGAI VNAIGYYVIG 430 440 450 460 470 480 LPIGIALMFA AKLGVIGLWS GIIICTTCQT TCFLAFIARL NWKRACQQAQ VHANLKVNVA 490 500 510 520 530 540 LNSAVSHEPA HPVCPESHGE IMMTDLEKKD ETQLDQPMNQ QQALPIRPKD SNKLSGKQLA 550 560 LRRGLLLLGV VLVLVGGILV RVYIRIE
	Isoform 2 [UniParc]. Checksum: BF6B65414F86C3DC	FASTA	425	46,259
	10 20 30 40 50 60 MSDTSPQAGV LSRARLLQLR RHSSQRPERS GLAGLLERVG IVLPQIMTGI AANLVNALAN 70 80 90 100 110 120 YVFLYHLHLG VMGSALANTI SQFALAIFLF LYILWRRLHQ ATWGGWSWEC LQDWASFLRL 130 140 150 160 170 180 AIPSMLMLCI EWWAYEVGSF LSGILGMVEL GAQSITYELA IIVYMIPSGF SVAANVRVGN 190 200 210 220 230 240 ALGAGNIDQA KKSSAISLIV TELFAVTFCV LLLGCKDLVG YIFTTDRDIV ALVAQVIPIY 250 260 270 280 290 300 AVSHLFEGLA CTCGGILRGT GNQKVGAIVN AIGYYVIGLP IGIALMFAAK LGVIGLWSGI 310 320 330 340 350 360 IICTTCQTTC FLAFIARLNW KRACQQAQVH ANLKVNVALN SAVSHEPAHP VCPESHGEIM 370 380 390 400 410 420 MTDLEKKDET QLDQPMNQQQ ALPIRPKDSN KLSGKQLALR RGLLLLGVVL VLVGGILVRV YIRIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Liver and Tongue.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Kidney.
[4]	"A human transporter protein that mediates the final excretion step for toxic organic cations." Otsuka M., Matsumoto T., Morimoto R., Arioka S., Omote H., Moriyama Y. Proc. Natl. Acad. Sci. U.S.A. 102:17923-17928(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]	"Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations." Hiasa M., Matsumoto T., Komatsu T., Moriyama Y. Am. J. Physiol. 291:C678-C686(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter." Hiasa M., Matsumoto T., Komatsu T., Omote H., Moriyama Y. Am. J. Physiol. 293:C1437-C1444(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK004994 mRNA. Translation: BAB23729.1. AK009038 mRNA. Translation: BAB26040.1. AL669884 Genomic DNA. Translation: CAI25734.1. BC031436 mRNA. No translation available.
IPI	IPI00132092. IPI00280005.
RefSeq	NP_080459.2. NM_026183.5.
UniGene	Mm.100741.
3D structure databases
ProteinModelPortal	Q8K0H1.
SMR	Q8K0H1. Positions 31-476.
ModBase	Search...
Protein family/group databases
TCDB	2.A.66.1.18. multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) flippase superfamily.
PTM databases
PhosphoSite	Q8K0H1.
Proteomic databases
PaxDb	Q8K0H1.
PRIDE	Q8K0H1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000010267; ENSMUSP00000010267; ENSMUSG00000010122.
GeneID	67473.
KEGG	mmu:67473.
UCSC	uc007jhh.2. mouse. uc007jhi.2. mouse.
Organism-specific databases
CTD	55244.
MGI	MGI:1914723. Slc47a1.
Phylogenomic databases
eggNOG	COG0534.
GeneTree	ENSGT00390000015713.
HOGENOM	HOG000060313.
HOVERGEN	HBG056043.
InParanoid	Q8K0H1.
KO	K03327.
OMA	FASFHIF.
OrthoDB	EOG4H9XKK.
Gene expression databases
ArrayExpress	Q8K0H1.
Bgee	Q8K0H1.
CleanEx	MM_SLC47A1.
Genevestigator	Q8K0H1.
Family and domain databases
InterPro	IPR002528. MATE. [Graphical view]
Pfam	PF01554. MatE. 2 hits. [Graphical view]
TIGRFAMs	TIGR00797. matE. 1 hit.
ProtoNet	Search...
Other
NextBio	324686.
SOURCE	Search...

Entry information

Entry name

S47A1_MOUSE

Accession

Primary (citable) accession number: Q8K0H1
Secondary accession number(s): Q5SS45, Q9CQ64

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program