Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multidrug and toxin extrusion protein 1

Gene

Slc47a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes (By similarity).By similarity2 Publications

Kineticsi

  1. KM=0.41 mM for TEA1 Publication
  1. Vmax=0.6 nmol/min/mg enzyme toward TEA1 Publication

pH dependencei

Optimum pH is 8.0-8.5. Active from pH 6 to 8.5.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Protein family/group databases

TCDBi2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug and toxin extrusion protein 1
Short name:
MATE-1
Short name:
mMATE-1
Alternative name(s):
Solute carrier family 47 member 1
Gene namesi
Name:Slc47a1
Synonyms:Mate1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914723. Slc47a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 72ExtracellularSequence analysisAdd BLAST14
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Topological domaini94 – 120CytoplasmicSequence analysisAdd BLAST27
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 152ExtracellularSequence analysisAdd BLAST11
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 187CytoplasmicSequence analysisAdd BLAST14
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 216ExtracellularSequence analysis8
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 257CytoplasmicSequence analysisAdd BLAST20
Transmembranei258 – 277HelicalSequence analysisAdd BLAST20
Topological domaini278 – 295ExtracellularSequence analysisAdd BLAST18
Transmembranei296 – 316HelicalSequence analysisAdd BLAST21
Topological domaini317 – 336CytoplasmicSequence analysisAdd BLAST20
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 370ExtracellularSequence analysisAdd BLAST13
Transmembranei371 – 391HelicalSequence analysisAdd BLAST21
Topological domaini392 – 408CytoplasmicSequence analysisAdd BLAST17
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21
Topological domaini430 – 437ExtracellularSequence analysis8
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Topological domaini459 – 543CytoplasmicSequence analysisAdd BLAST85
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 567ExtracellularSequence analysis3

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3091264.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003128461 – 567Multidrug and toxin extrusion protein 1Add BLAST567

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei18PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8K0H1.
PeptideAtlasiQ8K0H1.
PRIDEiQ8K0H1.

PTM databases

iPTMnetiQ8K0H1.
PhosphoSitePlusiQ8K0H1.

Expressioni

Tissue specificityi

Predominantly expressed in kidney and liver. Also expressed in various cells, including brain glia-like cells and capillaries, pancreatic duct cells, urinary bladder epithelium, adrenal gland cortex, heart, stomach, small intestine, thyroid gland, testes, alpha cells of the islets of Langerhans, Leydig cells, and vitamin A-storing Ito cells. Expressed in heart, stomach, small intestine, bladder, thyroid gland, adrenal gland and testes (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000010122.
CleanExiMM_SLC47A1.
ExpressionAtlasiQ8K0H1. baseline and differential.
GenevisibleiQ8K0H1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010267.

Chemistry databases

BindingDBiQ8K0H1.

Structurei

3D structure databases

ProteinModelPortaliQ8K0H1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ8K0H1.
KOiK03327.
OMAiCDALQIT.
OrthoDBiEOG091G06X2.
PhylomeDBiQ8K0H1.
TreeFamiTF324441.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K0H1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERTEESAPG PGGADAASER RGLRCLLLPG FLEELRALLV LAGPAFLAQL
60 70 80 90 100
MMFLISFISS VFCGHLGKLE LDAVTLAIAV INVTGISVGH GLSSACDTLI
110 120 130 140 150
SQTYGSQNLK HVGVILQRGT LILLLCCFPC WALFINTEQI LLLFRQDPDV
160 170 180 190 200
SRLTQTYVMI FIPALPAAFL YTLQVKYLLN QGIVLPQIMT GIAANLVNAL
210 220 230 240 250
ANYVFLYHLH LGVMGSALAN TISQFALAIF LFLYILWRRL HQATWGGWSW
260 270 280 290 300
ECLQDWASFL RLAIPSMLML CIEWWAYEVG SFLSGILGMV ELGAQSITYE
310 320 330 340 350
LAIIVYMIPS GFSVAANVRV GNALGAGNID QAKKSSAISL IVTELFAVTF
360 370 380 390 400
CVLLLGCKDL VGYIFTTDRD IVALVAQVIP IYAVSHLFEG LACTCGGILR
410 420 430 440 450
GTGNQKVGAI VNAIGYYVIG LPIGIALMFA AKLGVIGLWS GIIICTTCQT
460 470 480 490 500
TCFLAFIARL NWKRACQQAQ VHANLKVNVA LNSAVSHEPA HPVCPESHGE
510 520 530 540 550
IMMTDLEKKD ETQLDQPMNQ QQALPIRPKD SNKLSGKQLA LRRGLLLLGV
560
VLVLVGGILV RVYIRIE
Length:567
Mass (Da):61,642
Last modified:December 4, 2007 - v2
Checksum:iEDAF1D3DB3BBD8F5
GO
Isoform 2 (identifier: Q8K0H1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.
     143-181: LFRQDPDVSR...TLQVKYLLNQ → MSDTSPQAGV...SGLAGLLERV

Note: No experimental confirmation available.
Show »
Length:425
Mass (Da):46,259
Checksum:iBF6B65414F86C3DC
GO

Sequence cautioni

The sequence BC031436 differs from that shown. Reason: Frameshift at position 510.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0299061 – 142Missing in isoform 2. 1 PublicationAdd BLAST142
Alternative sequenceiVSP_029907143 – 181LFRQD…YLLNQ → MSDTSPQAGVLSRARLLQLR RHSSQRPERSGLAGLLERV in isoform 2. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004994 mRNA. Translation: BAB23729.1.
AK009038 mRNA. Translation: BAB26040.1.
AL669884 Genomic DNA. Translation: CAI25734.1.
BC031436 mRNA. No translation available.
CCDSiCCDS24811.2. [Q8K0H1-1]
RefSeqiNP_080459.2. NM_026183.5. [Q8K0H1-1]
XP_011247491.1. XM_011249189.2. [Q8K0H1-2]
UniGeneiMm.100741.

Genome annotation databases

EnsembliENSMUST00000010267; ENSMUSP00000010267; ENSMUSG00000010122. [Q8K0H1-1]
GeneIDi67473.
KEGGimmu:67473.
UCSCiuc007jhh.2. mouse. [Q8K0H1-2]
uc007jhi.2. mouse. [Q8K0H1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004994 mRNA. Translation: BAB23729.1.
AK009038 mRNA. Translation: BAB26040.1.
AL669884 Genomic DNA. Translation: CAI25734.1.
BC031436 mRNA. No translation available.
CCDSiCCDS24811.2. [Q8K0H1-1]
RefSeqiNP_080459.2. NM_026183.5. [Q8K0H1-1]
XP_011247491.1. XM_011249189.2. [Q8K0H1-2]
UniGeneiMm.100741.

3D structure databases

ProteinModelPortaliQ8K0H1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010267.

Chemistry databases

BindingDBiQ8K0H1.
ChEMBLiCHEMBL3091264.

Protein family/group databases

TCDBi2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

iPTMnetiQ8K0H1.
PhosphoSitePlusiQ8K0H1.

Proteomic databases

PaxDbiQ8K0H1.
PeptideAtlasiQ8K0H1.
PRIDEiQ8K0H1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010267; ENSMUSP00000010267; ENSMUSG00000010122. [Q8K0H1-1]
GeneIDi67473.
KEGGimmu:67473.
UCSCiuc007jhh.2. mouse. [Q8K0H1-2]
uc007jhi.2. mouse. [Q8K0H1-1]

Organism-specific databases

CTDi55244.
MGIiMGI:1914723. Slc47a1.

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ8K0H1.
KOiK03327.
OMAiCDALQIT.
OrthoDBiEOG091G06X2.
PhylomeDBiQ8K0H1.
TreeFamiTF324441.

Enzyme and pathway databases

ReactomeiR-MMU-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

PROiQ8K0H1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010122.
CleanExiMM_SLC47A1.
ExpressionAtlasiQ8K0H1. baseline and differential.
GenevisibleiQ8K0H1. MM.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS47A1_MOUSE
AccessioniPrimary (citable) accession number: Q8K0H1
Secondary accession number(s): Q5SS45, Q9CQ64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.