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Q8K0H1

- S47A1_MOUSE

UniProt

Q8K0H1 - S47A1_MOUSE

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Protein

Multidrug and toxin extrusion protein 1

Gene

Slc47a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes (By similarity).By similarity

Kineticsi

  1. KM=0.41 mM for TEA1 Publication

Vmax=0.6 nmol/min/mg enzyme toward TEA1 Publication

pH dependencei

Optimum pH is 8.0-8.5. Active from pH 6 to 8.5.1 Publication

GO - Molecular functioni

  1. drug transmembrane transporter activity Source: Ensembl
  2. monovalent cation:proton antiporter activity Source: Ensembl

GO - Biological processi

  1. organic cation transport Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Protein family/group databases

TCDBi2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug and toxin extrusion protein 1
Short name:
MATE-1
Short name:
mMATE-1
Alternative name(s):
Solute carrier family 47 member 1
Gene namesi
Name:Slc47a1
Synonyms:Mate1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1914723. Slc47a1.

Subcellular locationi

Cell membrane 3 Publications; Multi-pass membrane protein 3 Publications
Note: Predominantly localized to the plasma membrane; at the brush border membranes of the proximal tubules (kidney) and at the bile caniculi (liver).

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei38 – 5821HelicalSequence AnalysisAdd
BLAST
Topological domaini59 – 7214ExtracellularSequence AnalysisAdd
BLAST
Transmembranei73 – 9321HelicalSequence AnalysisAdd
BLAST
Topological domaini94 – 12027CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence AnalysisAdd
BLAST
Topological domaini142 – 15211ExtracellularSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Topological domaini174 – 18714CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence AnalysisAdd
BLAST
Topological domaini209 – 2168ExtracellularSequence Analysis
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Topological domaini238 – 25720CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei258 – 27720HelicalSequence AnalysisAdd
BLAST
Topological domaini278 – 29518ExtracellularSequence AnalysisAdd
BLAST
Transmembranei296 – 31621HelicalSequence AnalysisAdd
BLAST
Topological domaini317 – 33620CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei337 – 35721HelicalSequence AnalysisAdd
BLAST
Topological domaini358 – 37013ExtracellularSequence AnalysisAdd
BLAST
Transmembranei371 – 39121HelicalSequence AnalysisAdd
BLAST
Topological domaini392 – 40817CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei409 – 42921HelicalSequence AnalysisAdd
BLAST
Topological domaini430 – 4378ExtracellularSequence Analysis
Transmembranei438 – 45821HelicalSequence AnalysisAdd
BLAST
Topological domaini459 – 54385CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei544 – 56421HelicalSequence AnalysisAdd
BLAST
Topological domaini565 – 5673ExtracellularSequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane-bounded vesicle Source: Ensembl
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 567567Multidrug and toxin extrusion protein 1PRO_0000312846Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8K0H1.
PaxDbiQ8K0H1.
PRIDEiQ8K0H1.

PTM databases

PhosphoSiteiQ8K0H1.

Expressioni

Tissue specificityi

Predominantly expressed in kidney and liver. Also expressed in various cells, including brain glia-like cells and capillaries, pancreatic duct cells, urinary bladder epithelium, adrenal gland cortex, heart, stomach, small intestine, thyroid gland, testes, alpha cells of the islets of Langerhans, Leydig cells, and vitamin A-storing Ito cells. Expressed in heart, stomach, small intestine, bladder, thyroid gland, adrenal gland and testes (at protein level).1 Publication

Gene expression databases

BgeeiQ8K0H1.
CleanExiMM_SLC47A1.
ExpressionAtlasiQ8K0H1. baseline and differential.
GenevestigatoriQ8K0H1.

Structurei

3D structure databases

ProteinModelPortaliQ8K0H1.
SMRiQ8K0H1. Positions 31-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0534.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ8K0H1.
KOiK03327.
OMAiFPRYLVM.
OrthoDBiEOG7RRF71.
PhylomeDBiQ8K0H1.
TreeFamiTF324441.

Family and domain databases

InterProiIPR002528. MATE.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K0H1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERTEESAPG PGGADAASER RGLRCLLLPG FLEELRALLV LAGPAFLAQL
60 70 80 90 100
MMFLISFISS VFCGHLGKLE LDAVTLAIAV INVTGISVGH GLSSACDTLI
110 120 130 140 150
SQTYGSQNLK HVGVILQRGT LILLLCCFPC WALFINTEQI LLLFRQDPDV
160 170 180 190 200
SRLTQTYVMI FIPALPAAFL YTLQVKYLLN QGIVLPQIMT GIAANLVNAL
210 220 230 240 250
ANYVFLYHLH LGVMGSALAN TISQFALAIF LFLYILWRRL HQATWGGWSW
260 270 280 290 300
ECLQDWASFL RLAIPSMLML CIEWWAYEVG SFLSGILGMV ELGAQSITYE
310 320 330 340 350
LAIIVYMIPS GFSVAANVRV GNALGAGNID QAKKSSAISL IVTELFAVTF
360 370 380 390 400
CVLLLGCKDL VGYIFTTDRD IVALVAQVIP IYAVSHLFEG LACTCGGILR
410 420 430 440 450
GTGNQKVGAI VNAIGYYVIG LPIGIALMFA AKLGVIGLWS GIIICTTCQT
460 470 480 490 500
TCFLAFIARL NWKRACQQAQ VHANLKVNVA LNSAVSHEPA HPVCPESHGE
510 520 530 540 550
IMMTDLEKKD ETQLDQPMNQ QQALPIRPKD SNKLSGKQLA LRRGLLLLGV
560
VLVLVGGILV RVYIRIE
Length:567
Mass (Da):61,642
Last modified:December 4, 2007 - v2
Checksum:iEDAF1D3DB3BBD8F5
GO
Isoform 2 (identifier: Q8K0H1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.
     143-181: LFRQDPDVSR...TLQVKYLLNQ → MSDTSPQAGV...SGLAGLLERV

Note: No experimental confirmation available.

Show »
Length:425
Mass (Da):46,259
Checksum:iBF6B65414F86C3DC
GO

Sequence cautioni

The sequence BC031436 differs from that shown. Reason: Frameshift at position 510. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 142142Missing in isoform 2. 1 PublicationVSP_029906Add
BLAST
Alternative sequencei143 – 18139LFRQD…YLLNQ → MSDTSPQAGVLSRARLLQLR RHSSQRPERSGLAGLLERV in isoform 2. 1 PublicationVSP_029907Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004994 mRNA. Translation: BAB23729.1.
AK009038 mRNA. Translation: BAB26040.1.
AL669884 Genomic DNA. Translation: CAI25734.1.
BC031436 mRNA. No translation available.
CCDSiCCDS24811.2. [Q8K0H1-1]
RefSeqiNP_080459.2. NM_026183.5. [Q8K0H1-1]
XP_006534055.1. XM_006533992.1. [Q8K0H1-2]
UniGeneiMm.100741.

Genome annotation databases

EnsembliENSMUST00000010267; ENSMUSP00000010267; ENSMUSG00000010122. [Q8K0H1-1]
GeneIDi67473.
KEGGimmu:67473.
UCSCiuc007jhh.2. mouse. [Q8K0H1-2]
uc007jhi.2. mouse. [Q8K0H1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004994 mRNA. Translation: BAB23729.1 .
AK009038 mRNA. Translation: BAB26040.1 .
AL669884 Genomic DNA. Translation: CAI25734.1 .
BC031436 mRNA. No translation available.
CCDSi CCDS24811.2. [Q8K0H1-1 ]
RefSeqi NP_080459.2. NM_026183.5. [Q8K0H1-1 ]
XP_006534055.1. XM_006533992.1. [Q8K0H1-2 ]
UniGenei Mm.100741.

3D structure databases

ProteinModelPortali Q8K0H1.
SMRi Q8K0H1. Positions 31-476.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL3091264.

Protein family/group databases

TCDBi 2.A.66.1.18. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

PhosphoSitei Q8K0H1.

Proteomic databases

MaxQBi Q8K0H1.
PaxDbi Q8K0H1.
PRIDEi Q8K0H1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000010267 ; ENSMUSP00000010267 ; ENSMUSG00000010122 . [Q8K0H1-1 ]
GeneIDi 67473.
KEGGi mmu:67473.
UCSCi uc007jhh.2. mouse. [Q8K0H1-2 ]
uc007jhi.2. mouse. [Q8K0H1-1 ]

Organism-specific databases

CTDi 55244.
MGIi MGI:1914723. Slc47a1.

Phylogenomic databases

eggNOGi COG0534.
GeneTreei ENSGT00390000015713.
HOGENOMi HOG000060313.
HOVERGENi HBG056043.
InParanoidi Q8K0H1.
KOi K03327.
OMAi FPRYLVM.
OrthoDBi EOG7RRF71.
PhylomeDBi Q8K0H1.
TreeFami TF324441.

Enzyme and pathway databases

Reactomei REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

NextBioi 324686.
PROi Q8K0H1.
SOURCEi Search...

Gene expression databases

Bgeei Q8K0H1.
CleanExi MM_SLC47A1.
ExpressionAtlasi Q8K0H1. baseline and differential.
Genevestigatori Q8K0H1.

Family and domain databases

InterProi IPR002528. MATE.
[Graphical view ]
Pfami PF01554. MatE. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00797. matE. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Liver and Tongue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Kidney.
  4. "A human transporter protein that mediates the final excretion step for toxic organic cations."
    Otsuka M., Matsumoto T., Morimoto R., Arioka S., Omote H., Moriyama Y.
    Proc. Natl. Acad. Sci. U.S.A. 102:17923-17928(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  5. "Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations."
    Hiasa M., Matsumoto T., Komatsu T., Moriyama Y.
    Am. J. Physiol. 291:C678-C686(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  6. "Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter."
    Hiasa M., Matsumoto T., Komatsu T., Omote H., Moriyama Y.
    Am. J. Physiol. 293:C1437-C1444(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiS47A1_MOUSE
AccessioniPrimary (citable) accession number: Q8K0H1
Secondary accession number(s): Q5SS45, Q9CQ64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3