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Protein

Hyaluronan-binding protein 2

Gene

Habp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei360 – 3601Charge relay systemBy similarity
Active sitei409 – 4091Charge relay systemBy similarity
Active sitei507 – 5071Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.033.

Names & Taxonomyi

Protein namesi
Gene namesi
Name:Habp2
Synonyms:Phbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1196378. Habp2.

Subcellular locationi

  • Secreted

  • Note: Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 311288Hyaluronan-binding protein 2 50 kDa heavy chainPRO_0000027903Add
BLAST
Chaini27 – 311285Hyaluronan-binding protein 2 50 kDa heavy chain alternate formPRO_0000027904Add
BLAST
Chaini312 – 558247Hyaluronan-binding protein 2 27 kDa light chainPRO_0000027905Add
BLAST
Chaini318 – 558241Hyaluronan-binding protein 2 27 kDa light chain alternate formPRO_0000027906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 86By similarity
Disulfide bondi80 ↔ 95By similarity
Disulfide bondi97 ↔ 106By similarity
Disulfide bondi113 ↔ 123By similarity
Disulfide bondi118 ↔ 134By similarity
Disulfide bondi136 ↔ 145By similarity
Disulfide bondi152 ↔ 163By similarity
Disulfide bondi157 ↔ 174By similarity
Disulfide bondi176 ↔ 185By similarity
Disulfide bondi192 ↔ 274By similarity
Disulfide bondi213 ↔ 255By similarity
Disulfide bondi244 ↔ 269By similarity
Disulfide bondi299 ↔ 433Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi345 ↔ 361By similarity
Disulfide bondi445 ↔ 513By similarity
Disulfide bondi475 ↔ 491By similarity
Disulfide bondi503 ↔ 531By similarity

Post-translational modificationi

Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-311 or Lys-317 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Arg-168 or Arg-169 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-478 to give rise to inactive 17 kDa and 8 kDa fragments.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei167 – 1682Cleavage
Sitei168 – 1692Cleavage
Sitei478 – 4792Cleavage

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

MaxQBiQ8K0D2.
PRIDEiQ8K0D2.

PTM databases

PhosphoSiteiQ8K0D2.

Expressioni

Tissue specificityi

Liver and kidney.1 Publication

Gene expression databases

BgeeiQ8K0D2.
CleanExiMM_HABP2.

Interactioni

Subunit structurei

Heterodimer; disulfide-linked. Heterodimer of a 50 kDa heavy and a 27 kDa light chain linked by a disulfide bond.

Structurei

3D structure databases

ProteinModelPortaliQ8K0D2.
SMRiQ8K0D2. Positions 74-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 10737EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini109 – 14638EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini148 – 18639EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini191 – 27484KringlePROSITE-ProRule annotationAdd
BLAST
Domaini312 – 553242Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000237314.
HOVERGENiHBG106385.
InParanoidiQ8K0D2.
KOiK08648.
PhylomeDBiQ8K0D2.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K0D2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFVRMLVFRV LLLIALVGKS VIGLSLMSFI APPDPDWTPD DYYYSYEQSS
60 70 80 90 100
PDEDPSVTQT TPENPDWYYE DDDPCQSNPC EHGGDCIIRG DTFSCSCPAP
110 120 130 140 150
FSGSRCQTAQ NKCKDNPCVH GDCLITQKHP YYRCACKYPY TGPDCSKVLP
160 170 180 190 200
ACRPNPCQNG GVCSRHRRRS RFTCACPDQY KGKFCEIGPD DCYVGDGYSY
210 220 230 240 250
RGKVSKTVNQ NPCLYWNSHL LLQETYNMFM EDAETHGIAE HNFCRNPDGD
260 270 280 290 300
HKPWCFVKVN SEKVKWEYCD VTVCPVPDTP NPVESLLEPV MELPGFESCG
310 320 330 340 350
KTEVAEHAVK RIYGGFKSTA GKHPWQVSLQ TSLPLTTSMP QGHFCGGALI
360 370 380 390 400
HPCWVLTAAH CTDINTKHLK VVLGDQDLKK TESHEQTFRV EKILKYSQYN
410 420 430 440 450
ERDEIPHNDI ALLKLKPVGG HCALESRYVK TVCLPSDPFP SGTECHISGW
460 470 480 490 500
GVTETGEGSR QLLDAKVKLI ANPLCNSRQL YDHTIDDSMI CAGNLQKPGS
510 520 530 540 550
DTCQGDSGGP LTCEKDGTYY VYGIVSWGQE CGKKPGVYTQ VTKFLNWIKT

TMHREAGL
Length:558
Mass (Da):62,357
Last modified:August 30, 2005 - v2
Checksum:i0580CB38F6708E41
GO
Isoform 2 (identifier: Q8K0D2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-72: Missing.

Note: No experimental confirmation available.
Show »
Length:521
Mass (Da):57,860
Checksum:i184D3F7935B202CD
GO

Sequence cautioni

The sequence AAH31775.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 7237Missing in isoform 2. 1 PublicationVSP_015395Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031775 mRNA. Translation: AAH31775.1. Different initiation.
PIRiJC5878.
RefSeqiNP_666213.1. NM_146101.1.
UniGeneiMm.25791.

Genome annotation databases

GeneIDi226243.
KEGGimmu:226243.
UCSCiuc008hyt.1. mouse. [Q8K0D2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031775 mRNA. Translation: AAH31775.1. Different initiation.
PIRiJC5878.
RefSeqiNP_666213.1. NM_146101.1.
UniGeneiMm.25791.

3D structure databases

ProteinModelPortaliQ8K0D2.
SMRiQ8K0D2. Positions 74-552.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.033.

PTM databases

PhosphoSiteiQ8K0D2.

Proteomic databases

MaxQBiQ8K0D2.
PRIDEiQ8K0D2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi226243.
KEGGimmu:226243.
UCSCiuc008hyt.1. mouse. [Q8K0D2-1]

Organism-specific databases

CTDi3026.
MGIiMGI:1196378. Habp2.

Phylogenomic databases

HOGENOMiHOG000237314.
HOVERGENiHBG106385.
InParanoidiQ8K0D2.
KOiK08648.
PhylomeDBiQ8K0D2.

Miscellaneous databases

ChiTaRSiHabp2. mouse.
NextBioi378060.
PROiQ8K0D2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K0D2.
CleanExiMM_HABP2.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the cDNA for a mouse homologue of human PHBP: a novel hyaluronan-binding protein."
    Hashimoto K., Tobe T., Sumiya J., Saguchi K., Sano Y., Nakano Y., Choi-Miura N.-H., Tomita M.
    Biol. Pharm. Bull. 20:1127-1130(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-558 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Kidney.
  3. "Proteolytic activation and inactivation of the serine protease activity of plasma hyaluronan binding protein."
    Choi-Miura N.H., Takahashi K., Yoda M., Saito K., Mazda T., Tomita M.
    Biol. Pharm. Bull. 24:448-452(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, POST-TRANSLATIONAL MODIFICATIONS.

Entry informationi

Entry nameiHABP2_MOUSE
AccessioniPrimary (citable) accession number: Q8K0D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: May 11, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.