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Q8K0C4 (CP51A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lanosterol 14-alpha demethylase
Short name=LDM
EC=1.14.13.70
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name=Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene names
Name:Cyp51a1
Synonyms:Cyp51
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.

Catalytic activity

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Endoplasmic reticulum membrane Potential. Microsome membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence AAF73986.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Lanosterol 14-alpha demethylase
PRO_0000376796

Regions

Transmembrane24 – 4421Helical; Potential

Sites

Metal binding4491Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict191E → A in AAF74562. Ref.1
Sequence conflict981F → L in AAF74562. Ref.1
Sequence conflict981F → L in AAF73986. Ref.2
Sequence conflict2351T → A in BAC27231. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8K0C4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 6A4BBA350F1D85C7

FASTA50356,776
        10         20         30         40         50         60 
MVLLGLLQSG GWVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH MVQLPAGAKS 

        70         80         90        100        110        120 
PPHIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 

       130        140        150        160        170        180 
KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA IFLEQKKIIK SGLNIAHFKQ YVPIIEKEAK 

       190        200        210        220        230        240 
EYFQSWGESG ERNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFTHAAWL 

       250        260        270        280        290        300 
LPAWLPLPSF RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDEE 

       310        320        330        340        350        360 
ISGMLIGLLL AGQHTSSTTS AWMGFFLAKD KPLQEKCYLE QKAVCGEDLP PLTYDQLKDL 

       370        380        390        400        410        420 
NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWAERLD 

       430        440        450        460        470        480 
FNPDRYLQDN PASGEKFAYV PFGAGRHRCV GENFAYVQIK TIWSTMLRLY EFDLINGYFP 

       490        500 
TVNYTTMIHT PENPVIRYKR RSK

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the mouse lanosterol 14alpha-demethylase (CYP51), a new member of the evolutionarily most conserved cytochrome P450 family."
Debeljak N., Horvat S., Vouk K., Lee M., Rozman D.
Arch. Biochem. Biophys. 379:37-45(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[2]"Molecular cloning and partial characterisation of the mouse Cyp51 cDNA."
Debeljak N., Horvat S., Komel R., Rozman D.
Pflugers Arch. 439:R7-R8(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: 129/Sv.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo, Placenta, Skin and Testis.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF204804 expand/collapse EMBL AC list , AF204796, AF204797, AF204798, AF204799, AF204800, AF204801, AF204802, AF204803 Genomic DNA. Translation: AAF74562.1.
AF166266 mRNA. Translation: AAF73986.1. Different initiation.
AK028355 mRNA. Translation: BAC25900.1.
AK028815 mRNA. Translation: BAC26134.1.
AK031059 mRNA. Translation: BAC27231.1.
AK076983 mRNA. Translation: BAC36548.1.
CH466600 Genomic DNA. Translation: EDL14627.1.
BC031813 mRNA. Translation: AAH31813.1.
IPIIPI00458711.
RefSeqNP_064394.2. NM_020010.2.
UniGeneMm.140158.

3D structure databases

HSSPHSSP built from PDB template 1U13 based on UniProtKB P0A512.
ProteinModelPortalQ8K0C4.
SMRQ8K0C4. Positions 59-502.
ModBaseSearch...

PTM databases

PhosphoSiteQ8K0C4.

Proteomic databases

PaxDbQ8K0C4.
PRIDEQ8K0C4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001507; ENSMUSP00000001507; ENSMUSG00000001467.
GeneID13121.
KEGGmmu:13121.
UCSCuc008wie.1. mouse.

Organism-specific databases

CTD13121.
MGIMGI:106040. Cyp51.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00660000095370.
HOGENOMHOG000042780.
InParanoidQ9JIP8.
KOK05917.
OMAWGDEGEI.
OrthoDBEOG4M0F1P.

Enzyme and pathway databases

UniPathwayUPA00770; UER00754.

Gene expression databases

ArrayExpressQ8K0C4.
BgeeQ8K0C4.
GenevestigatorQ8K0C4.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283158.
SOURCESearch...

Entry information

Entry nameCP51A_MOUSE
AccessionPrimary (citable) accession number: Q8K0C4
Secondary accession number(s): Q8BSQ7, Q9JIP8, Q9JIY3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 1, 2002
Last modified: May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents