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Q8K0C4

- CP51A_MOUSE

UniProt

Q8K0C4 - CP51A_MOUSE

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Protein

Lanosterol 14-alpha demethylase

Gene

Cyp51a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.By similarity

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

Heme group.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi449 – 4491Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: UniProtKB
  2. iron ion binding Source: InterPro
  3. sterol 14-demethylase activity Source: UniProtKB

GO - Biological processi

  1. cholesterol biosynthetic process Source: MGI
  2. cholesterol biosynthetic process via 24,25-dihydrolanosterol Source: Ensembl
  3. demethylation Source: GOC
  4. steroid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_198969. Activation of gene expression by SREBF (SREBP).
REACT_208531. Cholesterol biosynthesis.
REACT_227038. Endogenous sterols.
UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.70)
Short name:
LDM
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name:
Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene namesi
Name:Cyp51a1
Synonyms:Cyp51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:106040. Cyp51.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Lanosterol 14-alpha demethylasePRO_0000376796Add
BLAST

Proteomic databases

MaxQBiQ8K0C4.
PaxDbiQ8K0C4.
PRIDEiQ8K0C4.

PTM databases

PhosphoSiteiQ8K0C4.

Expressioni

Gene expression databases

BgeeiQ8K0C4.
ExpressionAtlasiQ8K0C4. baseline and differential.
GenevestigatoriQ8K0C4.

Interactioni

Protein-protein interaction databases

MINTiMINT-1853747.

Structurei

3D structure databases

ProteinModelPortaliQ8K0C4.
SMRiQ8K0C4. Positions 59-502.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00660000095370.
HOGENOMiHOG000042780.
InParanoidiQ8K0C4.
KOiK05917.
OMAiMIHTPHN.
OrthoDBiEOG7PZRX3.
PhylomeDBiQ8K0C4.
TreeFamiTF105091.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K0C4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLLGLLQSG GWVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH
60 70 80 90 100
MVQLPAGAKS PPHIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM
110 120 130 140 150
VGKTFTYLLG SDAAALLFNS KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA
160 170 180 190 200
IFLEQKKIIK SGLNIAHFKQ YVPIIEKEAK EYFQSWGESG ERNVFEALSE
210 220 230 240 250
LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFTHAAWL LPAWLPLPSF
260 270 280 290 300
RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDEE
310 320 330 340 350
ISGMLIGLLL AGQHTSSTTS AWMGFFLAKD KPLQEKCYLE QKAVCGEDLP
360 370 380 390 400
PLTYDQLKDL NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ
410 420 430 440 450
VCVSPTVNQR LKDSWAERLD FNPDRYLQDN PASGEKFAYV PFGAGRHRCV
460 470 480 490 500
GENFAYVQIK TIWSTMLRLY EFDLINGYFP TVNYTTMIHT PENPVIRYKR

RSK
Length:503
Mass (Da):56,776
Last modified:October 1, 2002 - v1
Checksum:i6A4BBA350F1D85C7
GO

Sequence cautioni

The sequence AAF73986.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191E → A in AAF74562. (PubMed:10864439)Curated
Sequence conflicti98 – 981F → L in AAF74562. (PubMed:10864439)Curated
Sequence conflicti98 – 981F → L in AAF73986. (PubMed:10653123)Curated
Sequence conflicti235 – 2351T → A in BAC27231. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF204804
, AF204796, AF204797, AF204798, AF204799, AF204800, AF204801, AF204802, AF204803 Genomic DNA. Translation: AAF74562.1.
AF166266 mRNA. Translation: AAF73986.1. Different initiation.
AK028355 mRNA. Translation: BAC25900.1.
AK028815 mRNA. Translation: BAC26134.1.
AK031059 mRNA. Translation: BAC27231.1.
AK076983 mRNA. Translation: BAC36548.1.
CH466600 Genomic DNA. Translation: EDL14627.1.
BC031813 mRNA. Translation: AAH31813.1.
CCDSiCCDS19071.1.
RefSeqiNP_064394.2. NM_020010.2.
UniGeneiMm.140158.

Genome annotation databases

EnsembliENSMUST00000001507; ENSMUSP00000001507; ENSMUSG00000001467.
GeneIDi13121.
KEGGimmu:13121.
UCSCiuc008wie.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF204804
, AF204796 , AF204797 , AF204798 , AF204799 , AF204800 , AF204801 , AF204802 , AF204803 Genomic DNA. Translation: AAF74562.1 .
AF166266 mRNA. Translation: AAF73986.1 . Different initiation.
AK028355 mRNA. Translation: BAC25900.1 .
AK028815 mRNA. Translation: BAC26134.1 .
AK031059 mRNA. Translation: BAC27231.1 .
AK076983 mRNA. Translation: BAC36548.1 .
CH466600 Genomic DNA. Translation: EDL14627.1 .
BC031813 mRNA. Translation: AAH31813.1 .
CCDSi CCDS19071.1.
RefSeqi NP_064394.2. NM_020010.2.
UniGenei Mm.140158.

3D structure databases

ProteinModelPortali Q8K0C4.
SMRi Q8K0C4. Positions 59-502.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1853747.

PTM databases

PhosphoSitei Q8K0C4.

Proteomic databases

MaxQBi Q8K0C4.
PaxDbi Q8K0C4.
PRIDEi Q8K0C4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001507 ; ENSMUSP00000001507 ; ENSMUSG00000001467 .
GeneIDi 13121.
KEGGi mmu:13121.
UCSCi uc008wie.1. mouse.

Organism-specific databases

CTDi 13121.
MGIi MGI:106040. Cyp51.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00660000095370.
HOGENOMi HOG000042780.
InParanoidi Q8K0C4.
KOi K05917.
OMAi MIHTPHN.
OrthoDBi EOG7PZRX3.
PhylomeDBi Q8K0C4.
TreeFami TF105091.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00754 .
Reactomei REACT_198969. Activation of gene expression by SREBF (SREBP).
REACT_208531. Cholesterol biosynthesis.
REACT_227038. Endogenous sterols.

Miscellaneous databases

NextBioi 283158.
PROi Q8K0C4.
SOURCEi Search...

Gene expression databases

Bgeei Q8K0C4.
ExpressionAtlasi Q8K0C4. baseline and differential.
Genevestigatori Q8K0C4.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse lanosterol 14alpha-demethylase (CYP51), a new member of the evolutionarily most conserved cytochrome P450 family."
    Debeljak N., Horvat S., Vouk K., Lee M., Rozman D.
    Arch. Biochem. Biophys. 379:37-45(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. "Molecular cloning and partial characterisation of the mouse Cyp51 cDNA."
    Debeljak N., Horvat S., Komel R., Rozman D.
    Pflugers Arch. 439:R7-R8(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Placenta, Skin and Testis.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiCP51A_MOUSE
AccessioniPrimary (citable) accession number: Q8K0C4
Secondary accession number(s): Q8BSQ7, Q9JIP8, Q9JIY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3