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Q8K0B2 (LMBD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable lysosomal cobalamin transporter
Alternative name(s):
LMBR1 domain-containing protein 1
Protein N90b
Gene names
Name:Lmbrd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length537 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors By similarity.

Subcellular location

Lysosome membrane; Multi-pass membrane protein By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the LIMR family. LMBRD1 subfamily.

Ontologies

Keywords
   Biological processTransport
   Cellular componentLysosome
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandCobalamin
Cobalt
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncobalamin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K0B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K0B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     19-20: LL → MQ
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8K0B2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 537537Probable lysosomal cobalamin transporter
PRO_0000260517

Regions

Topological domain1 – 77Extracellular Potential
Transmembrane8 – 2821Helical; Name=1; Potential
Topological domain29 – 4719Cytoplasmic Potential
Transmembrane48 – 6821Helical; Name=2; Potential
Topological domain69 – 9729Extracellular Potential
Transmembrane98 – 11821Helical; Name=3; Potential
Topological domain119 – 14123Cytoplasmic Potential
Transmembrane142 – 16221Helical; Name=4; Potential
Topological domain163 – 18523Extracellular Potential
Transmembrane186 – 20621Helical; Name=5; Potential
Topological domain207 – 30296Cytoplasmic Potential
Transmembrane303 – 32321Helical; Name=6; Potential
Topological domain324 – 36138Extracellular Potential
Transmembrane362 – 38221Helical; Name=7; Potential
Topological domain383 – 40523Cytoplasmic Potential
Transmembrane406 – 42621Helical; Name=8; Potential
Topological domain427 – 48357Extracellular Potential
Transmembrane484 – 50421Helical; Name=9; Potential
Topological domain505 – 53733Cytoplasmic Potential

Amino acid modifications

Modified residue2351Phosphothreonine Ref.4
Modified residue5251Phosphoserine By similarity
Modified residue5281Phosphoserine By similarity
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation3441N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 7070Missing in isoform 3.
VSP_021631
Alternative sequence1 – 1818Missing in isoform 2.
VSP_021632
Alternative sequence19 – 202LL → MQ in isoform 2.
VSP_021633

Experimental info

Sequence conflict621P → L in BAC27926. Ref.2
Sequence conflict1731K → Q in AAL38052. Ref.1
Sequence conflict1931S → P in AAL38052. Ref.1
Sequence conflict3861G → D in BAE31002. Ref.2
Sequence conflict3861G → D in BAE31829. Ref.2
Sequence conflict4961W → R in BAB22556. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7AAFF431479752F0

FASTA53761,062
        10         20         30         40         50         60 
MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS LAVALITSAL 

        70         80         90        100        110        120 
LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT LYSVILFCVF FWIPFVYFYY 

       130        140        150        160        170        180 
EEKDEDDASK CTQIKTALKY TLGFVVICAL LLLVGAFVPL HLPNNNNSTE WEKVKLLFED 

       190        200        210        220        230        240 
LGTGQGLAAL SFSISSLTLI GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE 

       250        260        270        280        290        300 
VEQHIQTIRS KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP 

       310        320        330        340        350        360 
LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN MLLPLLQTVF 

       370        380        390        400        410        420 
PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG RTRPQALLFL CMILLLIVLH 

       430        440        450        460        470        480 
TSYMIYSLAP QYVMYGSQNY LIESNITSDA HKGNSTLAVP KRCDADAPKD QCTVTRTYIF 

       490        500        510        520        530 
LHKFWFFSAA YYFGNWAFLV VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA 

« Hide

Isoform 2 [UniParc].

Checksum: 5F1EB67EA4D94D32
Show »

FASTA51959,264
Isoform 3 [UniParc].

Checksum: BC4647DF0C55E55A
Show »

FASTA46753,440

References

« Hide 'large scale' references
[1]Yao R., Wang Y., You M.
Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Strain: A/J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J.
Tissue: Bone marrow, Olfactory bulb, Spleen and Visual cortex.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: FVB/N-3.
Tissue: Eye and Mammary tumor.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY061989 mRNA. Translation: AAL38052.1.
AK003084 mRNA. Translation: BAB22556.1.
AK032560 mRNA. Translation: BAC27926.1.
AK152170 mRNA. Translation: BAE31002.1.
AK153237 mRNA. Translation: BAE31829.1.
AK158982 mRNA. Translation: BAE34756.1.
AK167080 mRNA. Translation: BAE39240.1.
BC025579 mRNA. Translation: AAH25579.1.
BC027774 mRNA. Translation: AAH27774.1.
BC031902 mRNA. Translation: AAH31902.1.
BC039053 mRNA. Translation: AAH39053.1.
RefSeqNP_080995.2. NM_026719.2.
XP_006496303.1. XM_006496240.1.
UniGeneMm.336563.

3D structure databases

ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K0B2.

Proteomic databases

PaxDbQ8K0B2.
PRIDEQ8K0B2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725. [Q8K0B2-1]
GeneID68421.
KEGGmmu:68421.
UCSCuc007amt.1. mouse. [Q8K0B2-1]

Organism-specific databases

CTD55788.
MGIMGI:1915671. Lmbrd1.

Phylogenomic databases

eggNOGNOG87911.
GeneTreeENSGT00390000002581.
HOGENOMHOG000068018.
HOVERGENHBG059142.
InParanoidQ9CW67.
KOK14617.
OMACTQIKTA.
OrthoDBEOG7BKCTF.
PhylomeDBQ8K0B2.
TreeFamTF329170.

Gene expression databases

BgeeQ8K0B2.
CleanExMM_LMBRD1.
GenevestigatorQ8K0B2.

Family and domain databases

InterProIPR006876. LMBR1-like_membr_prot.
[Graphical view]
PfamPF04791. LMBR1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio327146.
PROQ8K0B2.
SOURCESearch...

Entry information

Entry nameLMBD1_MOUSE
AccessionPrimary (citable) accession number: Q8K0B2
Secondary accession number(s): Q3U696 expand/collapse secondary AC list , Q8CCL7, Q8R3D6, Q8VH50, Q9CW67
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot