Q8K0B2 (LMBD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable lysosomal cobalamin transporter

Alternative name(s):
LMBR1 domain-containing protein 1
Protein N90b

Gene names

Name:

Lmbrd1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors By similarity.
Subcellular location	Lysosome membrane; Multi-pass membrane protein By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the LIMR family. LMBRD1 subfamily.

Ontologies

Keywords
Biological process	Transport
Cellular component	Lysosome Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Cobalamin Cobalt
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K0B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K0B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     19-20: LL → MQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8K0B2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 537	537	Probable lysosomal cobalamin transporter		PRO_0000260517
Regions
	Topological domain	1 – 7	7	Extracellular Potential
	Transmembrane	8 – 28	21	Helical; Name=1; Potential
	Topological domain	29 – 47	19	Cytoplasmic Potential
	Transmembrane	48 – 68	21	Helical; Name=2; Potential
	Topological domain	69 – 97	29	Extracellular Potential
	Transmembrane	98 – 118	21	Helical; Name=3; Potential
	Topological domain	119 – 141	23	Cytoplasmic Potential
	Transmembrane	142 – 162	21	Helical; Name=4; Potential
	Topological domain	163 – 185	23	Extracellular Potential
	Transmembrane	186 – 206	21	Helical; Name=5; Potential
	Topological domain	207 – 302	96	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Helical; Name=6; Potential
	Topological domain	324 – 361	38	Extracellular Potential
	Transmembrane	362 – 382	21	Helical; Name=7; Potential
	Topological domain	383 – 405	23	Cytoplasmic Potential
	Transmembrane	406 – 426	21	Helical; Name=8; Potential
	Topological domain	427 – 483	57	Extracellular Potential
	Transmembrane	484 – 504	21	Helical; Name=9; Potential
	Topological domain	505 – 537	33	Cytoplasmic Potential
Amino acid modifications
	Modified residue	235	1	Phosphothreonine Ref.4
	Modified residue	525	1	Phosphoserine By similarity
	Modified residue	528	1	Phosphoserine By similarity
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	344	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 70	70	Missing in isoform 3.		VSP_021631
	Alternative sequence	1 – 18	18	Missing in isoform 2.		VSP_021632
	Alternative sequence	19 – 20	2	LL → MQ in isoform 2.		VSP_021633
Experimental info
	Sequence conflict	62	1	P → L in BAC27926. Ref.2
	Sequence conflict	173	1	K → Q in AAL38052. Ref.1
	Sequence conflict	193	1	S → P in AAL38052. Ref.1
	Sequence conflict	386	1	G → D in BAE31002. Ref.2
	Sequence conflict	386	1	G → D in BAE31829. Ref.2
	Sequence conflict	496	1	W → R in BAB22556. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7AAFF431479752F0	FASTA	537	61,062
	10 20 30 40 50 60 MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS LAVALITSAL 70 80 90 100 110 120 LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT LYSVILFCVF FWIPFVYFYY 130 140 150 160 170 180 EEKDEDDASK CTQIKTALKY TLGFVVICAL LLLVGAFVPL HLPNNNNSTE WEKVKLLFED 190 200 210 220 230 240 LGTGQGLAAL SFSISSLTLI GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE 250 260 270 280 290 300 VEQHIQTIRS KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP 310 320 330 340 350 360 LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN MLLPLLQTVF 370 380 390 400 410 420 PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG RTRPQALLFL CMILLLIVLH 430 440 450 460 470 480 TSYMIYSLAP QYVMYGSQNY LIESNITSDA HKGNSTLAVP KRCDADAPKD QCTVTRTYIF 490 500 510 520 530 LHKFWFFSAA YYFGNWAFLV VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA
	Isoform 2 [UniParc]. Checksum: 5F1EB67EA4D94D32	FASTA	519	59,264
	10 20 30 40 50 60 MQAILAFCWV YVRKYQSQRE SEVVSTVTAI FSLAVALITS ALLPVDIFLV SYMKNQNGTF 70 80 90 100 110 120 KDWADANVTV QIENTVLYGY YTLYSVILFC VFFWIPFVYF YYEEKDEDDA SKCTQIKTAL 130 140 150 160 170 180 KYTLGFVVIC ALLLLVGAFV PLHLPNNNNS TEWEKVKLLF EDLGTGQGLA ALSFSISSLT 190 200 210 220 230 240 LIGMLAAITY TAYGMSALPL NLIKGTRSTA YERLENTEDI EEVEQHIQTI RSKSKDGRPL 250 260 270 280 290 300 PARDRRALKQ CEERLRTLRK RERHLEFIEN SWWTKFCGAL RPLKIIWGIF FILVALLFVI 310 320 330 340 350 360 SLFLSNLDKA LHSAGIDSGF IIFGTNLSNP LNMLLPLLQT VFPLDYILIT IIIMYFIFTS 370 380 390 400 410 420 MAGIRNIGIW FFWIRLYKIR RGRTRPQALL FLCMILLLIV LHTSYMIYSL APQYVMYGSQ 430 440 450 460 470 480 NYLIESNITS DAHKGNSTLA VPKRCDADAP KDQCTVTRTY IFLHKFWFFS AAYYFGNWAF 490 500 510 LVVFLIGLIV SCCKGKKSVI EGVDEDSDLS DDEPSAYSA
	Isoform 3 [UniParc]. Checksum: BC4647DF0C55E55A	FASTA	467	53,440
	10 20 30 40 50 60 MKNQNGTFKD WADANVTVQI ENTVLYGYYT LYSVILFCVF FWIPFVYFYY EEKDEDDASK 70 80 90 100 110 120 CTQIKTALKY TLGFVVICAL LLLVGAFVPL HLPNNNNSTE WEKVKLLFED LGTGQGLAAL 130 140 150 160 170 180 SFSISSLTLI GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE VEQHIQTIRS 190 200 210 220 230 240 KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP LKIIWGIFFI 250 260 270 280 290 300 LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN MLLPLLQTVF PLDYILITII 310 320 330 340 350 360 IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG RTRPQALLFL CMILLLIVLH TSYMIYSLAP 370 380 390 400 410 420 QYVMYGSQNY LIESNITSDA HKGNSTLAVP KRCDADAPKD QCTVTRTYIF LHKFWFFSAA 430 440 450 460 YYFGNWAFLV VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Yao R., Wang Y., You M. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Strain: A/J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J. Tissue: Bone marrow, Olfactory bulb, Spleen and Visual cortex.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: FVB/N-3. Tissue: Eye and Mammary tumor.
[4]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-235, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY061989 mRNA. Translation: AAL38052.1. AK003084 mRNA. Translation: BAB22556.1. AK032560 mRNA. Translation: BAC27926.1. AK152170 mRNA. Translation: BAE31002.1. AK153237 mRNA. Translation: BAE31829.1. AK158982 mRNA. Translation: BAE34756.1. AK167080 mRNA. Translation: BAE39240.1. BC025579 mRNA. Translation: AAH25579.1. BC027774 mRNA. Translation: AAH27774.1. BC031902 mRNA. Translation: AAH31902.1. BC039053 mRNA. Translation: AAH39053.1.
IPI	IPI00352845. IPI00808296. IPI00808442.
RefSeq	NP_080995.2. NM_026719.2.
UniGene	Mm.336563.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8K0B2.
Proteomic databases
PaxDb	Q8K0B2.
PRIDE	Q8K0B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725.
GeneID	68421.
KEGG	mmu:68421.
UCSC	uc007amt.1. mouse. uc007amu.1. mouse.
Organism-specific databases
CTD	55788.
MGI	MGI:1915671. Lmbrd1.
Phylogenomic databases
eggNOG	NOG87911.
GeneTree	ENSGT00390000002581.
HOGENOM	HOG000068018.
HOVERGEN	HBG059142.
InParanoid	Q9CW67.
KO	K14617.
OMA	ADAPEDQ.
OrthoDB	EOG4933HV.
Gene expression databases
Bgee	Q8K0B2.
CleanEx	MM_LMBRD1.
Genevestigator	Q8K0B2.
GermOnline	ENSMUSG00000073725. Mus musculus.
Family and domain databases
InterPro	IPR006876. LMBR1-like_membr_prot. [Graphical view]
Pfam	PF04791. LMBR1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	327146.
SOURCE	Search...

Entry information

Entry name

LMBD1_MOUSE

Accession

Primary (citable) accession number: Q8K0B2
Secondary accession number(s): Q3U696 , Q8CCL7, Q8R3D6, Q8VH50, Q9CW67

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program