Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8K0B2

- LMBD1_MOUSE

UniProt

Q8K0B2 - LMBD1_MOUSE

Protein

Probable lysosomal cobalamin transporter

Gene

Lmbrd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors By similarity.By similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. insulin receptor binding Source: MGI

    GO - Biological processi

    1. insulin receptor internalization Source: MGI
    2. negative regulation of glucose import Source: MGI
    3. negative regulation of insulin receptor signaling pathway Source: MGI
    4. negative regulation of protein kinase B signaling Source: MGI

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Cobalamin, Cobalt

    Enzyme and pathway databases

    ReactomeiREACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable lysosomal cobalamin transporter
    Alternative name(s):
    LMBR1 domain-containing protein 1
    Protein N90b
    Gene namesi
    Name:Lmbrd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1915671. Lmbrd1.

    Subcellular locationi

    Lysosome membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. clathrin-coated endocytic vesicle Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. lysosomal membrane Source: UniProtKB-SubCell
    4. lysosome Source: MGI
    5. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 537537Probable lysosomal cobalamin transporterPRO_0000260517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
    Modified residuei235 – 2351Phosphothreonine1 Publication
    Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
    Modified residuei525 – 5251PhosphoserineBy similarity
    Modified residuei528 – 5281PhosphoserineBy similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ8K0B2.
    PaxDbiQ8K0B2.
    PRIDEiQ8K0B2.

    PTM databases

    PhosphoSiteiQ8K0B2.

    Expressioni

    Gene expression databases

    BgeeiQ8K0B2.
    CleanExiMM_LMBRD1.
    GenevestigatoriQ8K0B2.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77ExtracellularSequence Analysis
    Topological domaini29 – 4719CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini69 – 9729ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini119 – 14123CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini163 – 18523ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini207 – 30296CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 36138ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini383 – 40523CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini427 – 48357ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini505 – 53733CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei48 – 6821Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei142 – 16221Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei186 – 20621Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei406 – 42621Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei484 – 50421Helical; Name=9Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LIMR family. LMBRD1 subfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG87911.
    GeneTreeiENSGT00390000002581.
    HOGENOMiHOG000068018.
    HOVERGENiHBG059142.
    InParanoidiQ9CW67.
    KOiK14617.
    OMAiCTQIKTA.
    OrthoDBiEOG7BKCTF.
    PhylomeDBiQ8K0B2.
    TreeFamiTF329170.

    Family and domain databases

    InterProiIPR006876. LMBR1-like_membr_prot.
    [Graphical view]
    PfamiPF04791. LMBR1. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K0B2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS    50
    LAVALITSAL LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT 100
    LYSVILFCVF FWIPFVYFYY EEKDEDDASK CTQIKTALKY TLGFVVICAL 150
    LLLVGAFVPL HLPNNNNSTE WEKVKLLFED LGTGQGLAAL SFSISSLTLI 200
    GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE VEQHIQTIRS 250
    KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP 300
    LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN 350
    MLLPLLQTVF PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG 400
    RTRPQALLFL CMILLLIVLH TSYMIYSLAP QYVMYGSQNY LIESNITSDA 450
    HKGNSTLAVP KRCDADAPKD QCTVTRTYIF LHKFWFFSAA YYFGNWAFLV 500
    VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA 537
    Length:537
    Mass (Da):61,062
    Last modified:October 1, 2002 - v1
    Checksum:i7AAFF431479752F0
    GO
    Isoform 2 (identifier: Q8K0B2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-18: Missing.
         19-20: LL → MQ

    Note: No experimental confirmation available.

    Show »
    Length:519
    Mass (Da):59,264
    Checksum:i5F1EB67EA4D94D32
    GO
    Isoform 3 (identifier: Q8K0B2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-70: Missing.

    Show »
    Length:467
    Mass (Da):53,440
    Checksum:iBC4647DF0C55E55A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621P → L in BAC27926. (PubMed:16141072)Curated
    Sequence conflicti173 – 1731K → Q in AAL38052. 1 PublicationCurated
    Sequence conflicti193 – 1931S → P in AAL38052. 1 PublicationCurated
    Sequence conflicti386 – 3861G → D in BAE31002. (PubMed:16141072)Curated
    Sequence conflicti386 – 3861G → D in BAE31829. (PubMed:16141072)Curated
    Sequence conflicti496 – 4961W → R in BAB22556. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7070Missing in isoform 3. 3 PublicationsVSP_021631Add
    BLAST
    Alternative sequencei1 – 1818Missing in isoform 2. 1 PublicationVSP_021632Add
    BLAST
    Alternative sequencei19 – 202LL → MQ in isoform 2. 1 PublicationVSP_021633

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY061989 mRNA. Translation: AAL38052.1.
    AK003084 mRNA. Translation: BAB22556.1.
    AK032560 mRNA. Translation: BAC27926.1.
    AK152170 mRNA. Translation: BAE31002.1.
    AK153237 mRNA. Translation: BAE31829.1.
    AK158982 mRNA. Translation: BAE34756.1.
    AK167080 mRNA. Translation: BAE39240.1.
    BC025579 mRNA. Translation: AAH25579.1.
    BC027774 mRNA. Translation: AAH27774.1.
    BC031902 mRNA. Translation: AAH31902.1.
    BC039053 mRNA. Translation: AAH39053.1.
    CCDSiCCDS35528.1. [Q8K0B2-1]
    RefSeqiNP_080995.2. NM_026719.2. [Q8K0B2-1]
    XP_006496303.1. XM_006496240.1. [Q8K0B2-3]
    UniGeneiMm.336563.

    Genome annotation databases

    EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725. [Q8K0B2-1]
    GeneIDi68421.
    KEGGimmu:68421.
    UCSCiuc007amt.1. mouse. [Q8K0B2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY061989 mRNA. Translation: AAL38052.1 .
    AK003084 mRNA. Translation: BAB22556.1 .
    AK032560 mRNA. Translation: BAC27926.1 .
    AK152170 mRNA. Translation: BAE31002.1 .
    AK153237 mRNA. Translation: BAE31829.1 .
    AK158982 mRNA. Translation: BAE34756.1 .
    AK167080 mRNA. Translation: BAE39240.1 .
    BC025579 mRNA. Translation: AAH25579.1 .
    BC027774 mRNA. Translation: AAH27774.1 .
    BC031902 mRNA. Translation: AAH31902.1 .
    BC039053 mRNA. Translation: AAH39053.1 .
    CCDSi CCDS35528.1. [Q8K0B2-1 ]
    RefSeqi NP_080995.2. NM_026719.2. [Q8K0B2-1 ]
    XP_006496303.1. XM_006496240.1. [Q8K0B2-3 ]
    UniGenei Mm.336563.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K0B2.

    Proteomic databases

    MaxQBi Q8K0B2.
    PaxDbi Q8K0B2.
    PRIDEi Q8K0B2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000095062 ; ENSMUSP00000092672 ; ENSMUSG00000073725 . [Q8K0B2-1 ]
    GeneIDi 68421.
    KEGGi mmu:68421.
    UCSCi uc007amt.1. mouse. [Q8K0B2-1 ]

    Organism-specific databases

    CTDi 55788.
    MGIi MGI:1915671. Lmbrd1.

    Phylogenomic databases

    eggNOGi NOG87911.
    GeneTreei ENSGT00390000002581.
    HOGENOMi HOG000068018.
    HOVERGENi HBG059142.
    InParanoidi Q9CW67.
    KOi K14617.
    OMAi CTQIKTA.
    OrthoDBi EOG7BKCTF.
    PhylomeDBi Q8K0B2.
    TreeFami TF329170.

    Enzyme and pathway databases

    Reactomei REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.

    Miscellaneous databases

    NextBioi 327146.
    PROi Q8K0B2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K0B2.
    CleanExi MM_LMBRD1.
    Genevestigatori Q8K0B2.

    Family and domain databases

    InterProi IPR006876. LMBR1-like_membr_prot.
    [Graphical view ]
    Pfami PF04791. LMBR1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Yao R., Wang Y., You M.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
      Strain: A/J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Bone marrow, Olfactory bulb, Spleen and Visual cortex.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: FVB/N-3.
      Tissue: Eye and Mammary tumor.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLMBD1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K0B2
    Secondary accession number(s): Q3U696
    , Q8CCL7, Q8R3D6, Q8VH50, Q9CW67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3