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Protein

Probable lysosomal cobalamin transporter

Gene

Lmbrd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors (By similarity).By similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. insulin receptor binding Source: MGI

GO - Biological processi

  1. insulin receptor internalization Source: MGI
  2. negative regulation of glucose import Source: MGI
  3. negative regulation of insulin receptor signaling pathway Source: MGI
  4. negative regulation of protein kinase B signaling Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Cobalamin, Cobalt

Enzyme and pathway databases

ReactomeiREACT_294704. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lysosomal cobalamin transporter
Alternative name(s):
LMBR1 domain-containing protein 1
Protein N90b
Gene namesi
Name:Lmbrd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915671. Lmbrd1.

Subcellular locationi

  1. Lysosome membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77ExtracellularSequence Analysis
Transmembranei8 – 2821Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini29 – 4719CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei48 – 6821Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini69 – 9729ExtracellularSequence AnalysisAdd
BLAST
Transmembranei98 – 11821Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini119 – 14123CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei142 – 16221Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini163 – 18523ExtracellularSequence AnalysisAdd
BLAST
Transmembranei186 – 20621Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini207 – 30296CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei303 – 32321Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini324 – 36138ExtracellularSequence AnalysisAdd
BLAST
Transmembranei362 – 38221Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini383 – 40523CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei406 – 42621Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini427 – 48357ExtracellularSequence AnalysisAdd
BLAST
Transmembranei484 – 50421Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini505 – 53733CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. clathrin-coated endocytic vesicle Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosomal membrane Source: UniProtKB-SubCell
  4. lysosome Source: MGI
  5. membrane Source: MGI
  6. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Probable lysosomal cobalamin transporterPRO_0000260517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Modified residuei235 – 2351Phosphothreonine1 Publication
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
Modified residuei525 – 5251PhosphoserineBy similarity
Modified residuei528 – 5281PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8K0B2.
PaxDbiQ8K0B2.
PRIDEiQ8K0B2.

PTM databases

PhosphoSiteiQ8K0B2.

Expressioni

Gene expression databases

BgeeiQ8K0B2.
CleanExiMM_LMBRD1.
GenevestigatoriQ8K0B2.

Family & Domainsi

Sequence similaritiesi

Belongs to the LIMR family. LMBRD1 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG87911.
GeneTreeiENSGT00390000002581.
HOGENOMiHOG000068018.
HOVERGENiHBG059142.
InParanoidiQ8K0B2.
KOiK14617.
OMAiCTQIKTA.
OrthoDBiEOG7BKCTF.
PhylomeDBiQ8K0B2.
TreeFamiTF329170.

Family and domain databases

InterProiIPR006876. LMBR1-like_membr_prot.
[Graphical view]
PfamiPF04791. LMBR1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K0B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS
60 70 80 90 100
LAVALITSAL LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT
110 120 130 140 150
LYSVILFCVF FWIPFVYFYY EEKDEDDASK CTQIKTALKY TLGFVVICAL
160 170 180 190 200
LLLVGAFVPL HLPNNNNSTE WEKVKLLFED LGTGQGLAAL SFSISSLTLI
210 220 230 240 250
GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE VEQHIQTIRS
260 270 280 290 300
KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP
310 320 330 340 350
LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN
360 370 380 390 400
MLLPLLQTVF PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG
410 420 430 440 450
RTRPQALLFL CMILLLIVLH TSYMIYSLAP QYVMYGSQNY LIESNITSDA
460 470 480 490 500
HKGNSTLAVP KRCDADAPKD QCTVTRTYIF LHKFWFFSAA YYFGNWAFLV
510 520 530
VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA
Length:537
Mass (Da):61,062
Last modified:October 1, 2002 - v1
Checksum:i7AAFF431479752F0
GO
Isoform 2 (identifier: Q8K0B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     19-20: LL → MQ

Note: No experimental confirmation available.

Show »
Length:519
Mass (Da):59,264
Checksum:i5F1EB67EA4D94D32
GO
Isoform 3 (identifier: Q8K0B2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:467
Mass (Da):53,440
Checksum:iBC4647DF0C55E55A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621P → L in BAC27926 (PubMed:16141072).Curated
Sequence conflicti173 – 1731K → Q in AAL38052 (Ref. 1) Curated
Sequence conflicti193 – 1931S → P in AAL38052 (Ref. 1) Curated
Sequence conflicti386 – 3861G → D in BAE31002 (PubMed:16141072).Curated
Sequence conflicti386 – 3861G → D in BAE31829 (PubMed:16141072).Curated
Sequence conflicti496 – 4961W → R in BAB22556 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070Missing in isoform 3. 3 PublicationsVSP_021631Add
BLAST
Alternative sequencei1 – 1818Missing in isoform 2. 1 PublicationVSP_021632Add
BLAST
Alternative sequencei19 – 202LL → MQ in isoform 2. 1 PublicationVSP_021633

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061989 mRNA. Translation: AAL38052.1.
AK003084 mRNA. Translation: BAB22556.1.
AK032560 mRNA. Translation: BAC27926.1.
AK152170 mRNA. Translation: BAE31002.1.
AK153237 mRNA. Translation: BAE31829.1.
AK158982 mRNA. Translation: BAE34756.1.
AK167080 mRNA. Translation: BAE39240.1.
BC025579 mRNA. Translation: AAH25579.1.
BC027774 mRNA. Translation: AAH27774.1.
BC031902 mRNA. Translation: AAH31902.1.
BC039053 mRNA. Translation: AAH39053.1.
CCDSiCCDS35528.1. [Q8K0B2-1]
RefSeqiNP_080995.2. NM_026719.2. [Q8K0B2-1]
XP_006496303.1. XM_006496240.1. [Q8K0B2-3]
UniGeneiMm.336563.

Genome annotation databases

EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725. [Q8K0B2-3]
ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725. [Q8K0B2-1]
GeneIDi68421.
KEGGimmu:68421.
UCSCiuc007amt.1. mouse. [Q8K0B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061989 mRNA. Translation: AAL38052.1.
AK003084 mRNA. Translation: BAB22556.1.
AK032560 mRNA. Translation: BAC27926.1.
AK152170 mRNA. Translation: BAE31002.1.
AK153237 mRNA. Translation: BAE31829.1.
AK158982 mRNA. Translation: BAE34756.1.
AK167080 mRNA. Translation: BAE39240.1.
BC025579 mRNA. Translation: AAH25579.1.
BC027774 mRNA. Translation: AAH27774.1.
BC031902 mRNA. Translation: AAH31902.1.
BC039053 mRNA. Translation: AAH39053.1.
CCDSiCCDS35528.1. [Q8K0B2-1]
RefSeqiNP_080995.2. NM_026719.2. [Q8K0B2-1]
XP_006496303.1. XM_006496240.1. [Q8K0B2-3]
UniGeneiMm.336563.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8K0B2.

Proteomic databases

MaxQBiQ8K0B2.
PaxDbiQ8K0B2.
PRIDEiQ8K0B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725. [Q8K0B2-3]
ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725. [Q8K0B2-1]
GeneIDi68421.
KEGGimmu:68421.
UCSCiuc007amt.1. mouse. [Q8K0B2-1]

Organism-specific databases

CTDi55788.
MGIiMGI:1915671. Lmbrd1.

Phylogenomic databases

eggNOGiNOG87911.
GeneTreeiENSGT00390000002581.
HOGENOMiHOG000068018.
HOVERGENiHBG059142.
InParanoidiQ8K0B2.
KOiK14617.
OMAiCTQIKTA.
OrthoDBiEOG7BKCTF.
PhylomeDBiQ8K0B2.
TreeFamiTF329170.

Enzyme and pathway databases

ReactomeiREACT_294704. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

NextBioi327146.
PROiQ8K0B2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K0B2.
CleanExiMM_LMBRD1.
GenevestigatoriQ8K0B2.

Family and domain databases

InterProiIPR006876. LMBR1-like_membr_prot.
[Graphical view]
PfamiPF04791. LMBR1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Yao R., Wang Y., You M.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: A/J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Olfactory bulb, Spleen and Visual cortex.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: FVB/N-3.
    Tissue: Eye and Mammary tumor.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLMBD1_MOUSE
AccessioniPrimary (citable) accession number: Q8K0B2
Secondary accession number(s): Q3U696
, Q8CCL7, Q8R3D6, Q8VH50, Q9CW67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2002
Last modified: April 1, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.