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Protein

Probable lysosomal cobalamin transporter

Gene

Lmbrd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors (By similarity).By similarity

GO - Molecular functioni

  • cobalamin binding Source: UniProtKB-KW
  • insulin receptor binding Source: MGI

GO - Biological processi

  • insulin receptor internalization Source: MGI
  • negative regulation of glucose import Source: MGI
  • negative regulation of insulin receptor signaling pathway Source: MGI
  • negative regulation of protein kinase B signaling Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Cobalamin, Cobalt

Enzyme and pathway databases

ReactomeiR-MMU-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lysosomal cobalamin transporter
Alternative name(s):
LMBR1 domain-containing protein 1
Protein N90b
Gene namesi
Name:Lmbrd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915671. Lmbrd1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7ExtracellularSequence analysis7
Transmembranei8 – 28Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini29 – 47CytoplasmicSequence analysisAdd BLAST19
Transmembranei48 – 68Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini69 – 97ExtracellularSequence analysisAdd BLAST29
Transmembranei98 – 118Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini119 – 141CytoplasmicSequence analysisAdd BLAST23
Transmembranei142 – 162Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini163 – 185ExtracellularSequence analysisAdd BLAST23
Transmembranei186 – 206Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini207 – 302CytoplasmicSequence analysisAdd BLAST96
Transmembranei303 – 323Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini324 – 361ExtracellularSequence analysisAdd BLAST38
Transmembranei362 – 382Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini383 – 405CytoplasmicSequence analysisAdd BLAST23
Transmembranei406 – 426Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini427 – 483ExtracellularSequence analysisAdd BLAST57
Transmembranei484 – 504Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini505 – 537CytoplasmicSequence analysisAdd BLAST33

GO - Cellular componenti

  • clathrin-coated endocytic vesicle Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: UniProtKB-SubCell
  • lysosome Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002605171 – 537Probable lysosomal cobalamin transporterAdd BLAST537

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Glycosylationi167N-linked (GlcNAc...)Sequence analysis1
Modified residuei235PhosphothreonineCombined sources1
Glycosylationi344N-linked (GlcNAc...)Sequence analysis1
Glycosylationi445N-linked (GlcNAc...)Sequence analysis1
Glycosylationi454N-linked (GlcNAc...)Sequence analysis1
Modified residuei525PhosphoserineCombined sources1
Modified residuei528PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8K0B2.
PeptideAtlasiQ8K0B2.
PRIDEiQ8K0B2.

PTM databases

iPTMnetiQ8K0B2.
PhosphoSitePlusiQ8K0B2.
SwissPalmiQ8K0B2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000073725.
CleanExiMM_LMBRD1.
ExpressionAtlasiQ8K0B2. baseline and differential.
GenevisibleiQ8K0B2. MM.

Interactioni

GO - Molecular functioni

  • insulin receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092672.

Structurei

3D structure databases

ProteinModelPortaliQ8K0B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LIMR family. LMBRD1 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFHV. Eukaryota.
ENOG410XPKG. LUCA.
GeneTreeiENSGT00390000002581.
HOGENOMiHOG000068018.
HOVERGENiHBG059142.
InParanoidiQ8K0B2.
KOiK14617.
OMAiCTQIKTA.
OrthoDBiEOG091G0BP6.
PhylomeDBiQ8K0B2.
TreeFamiTF329170.

Family and domain databases

InterProiIPR006876. LMBR1-like_membr_prot.
[Graphical view]
PfamiPF04791. LMBR1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K0B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS
60 70 80 90 100
LAVALITSAL LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT
110 120 130 140 150
LYSVILFCVF FWIPFVYFYY EEKDEDDASK CTQIKTALKY TLGFVVICAL
160 170 180 190 200
LLLVGAFVPL HLPNNNNSTE WEKVKLLFED LGTGQGLAAL SFSISSLTLI
210 220 230 240 250
GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE VEQHIQTIRS
260 270 280 290 300
KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP
310 320 330 340 350
LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN
360 370 380 390 400
MLLPLLQTVF PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG
410 420 430 440 450
RTRPQALLFL CMILLLIVLH TSYMIYSLAP QYVMYGSQNY LIESNITSDA
460 470 480 490 500
HKGNSTLAVP KRCDADAPKD QCTVTRTYIF LHKFWFFSAA YYFGNWAFLV
510 520 530
VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA
Length:537
Mass (Da):61,062
Last modified:October 1, 2002 - v1
Checksum:i7AAFF431479752F0
GO
Isoform 2 (identifier: Q8K0B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     19-20: LL → MQ

Note: No experimental confirmation available.
Show »
Length:519
Mass (Da):59,264
Checksum:i5F1EB67EA4D94D32
GO
Isoform 3 (identifier: Q8K0B2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:467
Mass (Da):53,440
Checksum:iBC4647DF0C55E55A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62P → L in BAC27926 (PubMed:16141072).Curated1
Sequence conflicti173K → Q in AAL38052 (Ref. 1) Curated1
Sequence conflicti193S → P in AAL38052 (Ref. 1) Curated1
Sequence conflicti386G → D in BAE31002 (PubMed:16141072).Curated1
Sequence conflicti386G → D in BAE31829 (PubMed:16141072).Curated1
Sequence conflicti496W → R in BAB22556 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0216311 – 70Missing in isoform 3. 3 PublicationsAdd BLAST70
Alternative sequenceiVSP_0216321 – 18Missing in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_02163319 – 20LL → MQ in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061989 mRNA. Translation: AAL38052.1.
AK003084 mRNA. Translation: BAB22556.1.
AK032560 mRNA. Translation: BAC27926.1.
AK152170 mRNA. Translation: BAE31002.1.
AK153237 mRNA. Translation: BAE31829.1.
AK158982 mRNA. Translation: BAE34756.1.
AK167080 mRNA. Translation: BAE39240.1.
BC025579 mRNA. Translation: AAH25579.1.
BC027774 mRNA. Translation: AAH27774.1.
BC031902 mRNA. Translation: AAH31902.1.
BC039053 mRNA. Translation: AAH39053.1.
CCDSiCCDS35528.1. [Q8K0B2-1]
CCDS78556.1. [Q8K0B2-3]
RefSeqiNP_001297412.1. NM_001310483.1. [Q8K0B2-3]
NP_080995.2. NM_026719.2. [Q8K0B2-1]
UniGeneiMm.336563.

Genome annotation databases

EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725. [Q8K0B2-3]
ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725. [Q8K0B2-1]
GeneIDi68421.
KEGGimmu:68421.
UCSCiuc007amt.1. mouse. [Q8K0B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061989 mRNA. Translation: AAL38052.1.
AK003084 mRNA. Translation: BAB22556.1.
AK032560 mRNA. Translation: BAC27926.1.
AK152170 mRNA. Translation: BAE31002.1.
AK153237 mRNA. Translation: BAE31829.1.
AK158982 mRNA. Translation: BAE34756.1.
AK167080 mRNA. Translation: BAE39240.1.
BC025579 mRNA. Translation: AAH25579.1.
BC027774 mRNA. Translation: AAH27774.1.
BC031902 mRNA. Translation: AAH31902.1.
BC039053 mRNA. Translation: AAH39053.1.
CCDSiCCDS35528.1. [Q8K0B2-1]
CCDS78556.1. [Q8K0B2-3]
RefSeqiNP_001297412.1. NM_001310483.1. [Q8K0B2-3]
NP_080995.2. NM_026719.2. [Q8K0B2-1]
UniGeneiMm.336563.

3D structure databases

ProteinModelPortaliQ8K0B2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092672.

PTM databases

iPTMnetiQ8K0B2.
PhosphoSitePlusiQ8K0B2.
SwissPalmiQ8K0B2.

Proteomic databases

PaxDbiQ8K0B2.
PeptideAtlasiQ8K0B2.
PRIDEiQ8K0B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725. [Q8K0B2-3]
ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725. [Q8K0B2-1]
GeneIDi68421.
KEGGimmu:68421.
UCSCiuc007amt.1. mouse. [Q8K0B2-1]

Organism-specific databases

CTDi55788.
MGIiMGI:1915671. Lmbrd1.

Phylogenomic databases

eggNOGiENOG410IFHV. Eukaryota.
ENOG410XPKG. LUCA.
GeneTreeiENSGT00390000002581.
HOGENOMiHOG000068018.
HOVERGENiHBG059142.
InParanoidiQ8K0B2.
KOiK14617.
OMAiCTQIKTA.
OrthoDBiEOG091G0BP6.
PhylomeDBiQ8K0B2.
TreeFamiTF329170.

Enzyme and pathway databases

ReactomeiR-MMU-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

PROiQ8K0B2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073725.
CleanExiMM_LMBRD1.
ExpressionAtlasiQ8K0B2. baseline and differential.
GenevisibleiQ8K0B2. MM.

Family and domain databases

InterProiIPR006876. LMBR1-like_membr_prot.
[Graphical view]
PfamiPF04791. LMBR1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLMBD1_MOUSE
AccessioniPrimary (citable) accession number: Q8K0B2
Secondary accession number(s): Q3U696
, Q8CCL7, Q8R3D6, Q8VH50, Q9CW67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.