Q8K097 (LFG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein lifeguard 2

Alternative name(s):
Fas apoptotic inhibitory molecule 2
Neural membrane protein 35

Gene names

Name:	Faim2
Synonyms:	Kiaa0950, Lfg, Lfg2, Nmp35

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. Plays a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development. Ref.5 Ref.8 Ref.9
Subunit structure	Interacts with FAS/TNFRSF6 and BAX By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Membrane raft. Cell junction › synapse › postsynaptic cell membrane By similarity Ref.5.
Tissue specificity	Brain. Highly expressed in cerebellum, also found in cortex, olfactory bulb, and hippocampus. Ref.5 Ref.9
Disruption phenotype	Mice show reduced cerebellar size and internal granular layer (IGL) thickness in early developmental stages, delayed Purkinje Cell (PC) development, with an abnormal morphology and reduced cellular density, increased caspase-8 and caspase-3 activity in PCs and higher sensitivity to Fas-mediated apoptosis. Ref.9
Sequence similarities	Belongs to the BI1 family. LFG subfamily.
Sequence caution	The sequence BAC98056.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cerebellar Purkinje cell differentiation Inferred from mutant phenotype Ref.9. Source: UniProtKB cerebellar granular layer development Inferred from mutant phenotype Ref.9. Source: UniProtKB regulation of neuron apoptotic process Inferred from mutant phenotype Ref.5Ref.9. Source: UniProtKB
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay Ref.5. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K097-1)

Also known as: Long; LFG-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K097-2)

Also known as: Short; LFG-S;

The sequence of this isoform differs from the canonical sequence as follows:
     72-84: SGSSGYEGGFPAG → R

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Protein lifeguard 2		PRO_0000179088
Regions
	Transmembrane	107 – 127	21	Helical; Potential
	Transmembrane	139 – 159	21	Helical; Potential
	Transmembrane	166 – 186	21	Helical; Potential
	Transmembrane	195 – 215	21	Helical; Potential
	Transmembrane	226 – 246	21	Helical; Potential
	Transmembrane	252 – 272	21	Helical; Potential
	Transmembrane	291 – 311	21	Helical; Potential
Amino acid modifications
	Modified residue	39	1	Phosphotyrosine Ref.6
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	72 – 84	13	SGSSG…GFPAG → R in isoform 2.		VSP_008994
Experimental info
	Sequence conflict	256	1	A → T in BAC98056. Ref.2
	Sequence conflict	312	1	F → Y in AAM46781. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) (LFG-L) [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A62D51BA49BCDB15	FASTA	317	35,258
	10 20 30 40 50 60 MTQGKLSVAN KAPGTEGQQH QANGEKKDAP AVPSAPPSYE EATSGEGLKA GTFPQGPTAV 70 80 90 100 110 120 PLHPSWAYVD PSGSSGYEGG FPAGHHEHFT TFSWDDQKVR RLFIRKVYTI LLVQLLVTLA 130 140 150 160 170 180 VVALFTFCDV VKDYVQANPG WYWASYAVFF ATYLTLACCS GPRRHFPWNL ILLTIFTLSM 190 200 210 220 230 240 AYLTGMLSSY YNTTSVLLCL VITALVCLSV TIFSFQTKFD FTSCQGVLFV LLMTLFFSGL 250 260 270 280 290 300 LLAVLLPFQY VPWLHAVYAV LGAGVFTLFL AFDTQLLMGN RRHSLSPEEY IFGALNIYLD 310 IIYIFTFFLQ LFGTNRE
	Isoform 2 (Short) (LFG-S) [UniParc]. Checksum: 8793FA6D2464F6B8	FASTA	305	34,260
	10 20 30 40 50 60 MTQGKLSVAN KAPGTEGQQH QANGEKKDAP AVPSAPPSYE EATSGEGLKA GTFPQGPTAV 70 80 90 100 110 120 PLHPSWAYVD PRHHEHFTTF SWDDQKVRRL FIRKVYTILL VQLLVTLAVV ALFTFCDVVK 130 140 150 160 170 180 DYVQANPGWY WASYAVFFAT YLTLACCSGP RRHFPWNLIL LTIFTLSMAY LTGMLSSYYN 190 200 210 220 230 240 TTSVLLCLVI TALVCLSVTI FSFQTKFDFT SCQGVLFVLL MTLFFSGLLL AVLLPFQYVP 250 260 270 280 290 300 WLHAVYAVLG AGVFTLFLAF DTQLLMGNRR HSLSPEEYIF GALNIYLDII YIFTFFLQLF GTNRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the long form of mouse lifeguard (LFG-L), an anti Fas-apoptotic molecule." Sole C., Segura M.F., Bayascas J.R., Comella J.X. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Retina.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Hippocampus and Visual cortex.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Retina.
[5]	"Lifeguard/neuronal membrane protein 35 regulates Fas ligand-mediated apoptosis in neurons via microdomain recruitment." Fernandez M., Segura M.F., Sole C., Colino A., Comella J.X., Cena V. J. Neurochem. 103:190-203(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-39, MASS SPECTROMETRY. Tissue: Brain.
[7]	"LFG: a candidate apoptosis regulatory gene family." Hu L., Smith T.F., Goldberger G. Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Fas/CD95 regulatory protein Faim2 is neuroprotective after transient brain ischemia." Reich A., Spering C., Gertz K., Harms C., Gerhardt E., Kronenberg G., Nave K.A., Schwab M., Tauber S.C., Drinkut A., Harms K., Beier C.P., Voigt A., Goebbels S., Endres M., Schulz J.B. J. Neurosci. 31:225-233(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Antiapoptotic protein Lifeguard is required for survival and maintenance of Purkinje and granular cells." Hurtado de Mendoza T., Perez-Garcia C.G., Kroll T.T., Hoong N.H., O'Leary D.D., Verma I.M. Proc. Natl. Acad. Sci. U.S.A. 108:17189-17194(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF468028 mRNA. Translation: AAM46781.1. AK013476 mRNA. Translation: BAB28874.1. AK030513 mRNA. Translation: BAC26999.1. AK129246 mRNA. Translation: BAC98056.1. Different initiation. AK158960 mRNA. Translation: BAE34742.1. BC032278 mRNA. Translation: AAH32278.1.
IPI	IPI00408985. IPI00467229.
RefSeq	NP_001033747.1. NM_001038658.2. NP_082500.2. NM_028224.4.
UniGene	Mm.39760.
3D structure databases
ProteinModelPortal	Q8K097.
ModBase	Search...
PTM databases
PhosphoSite	Q8K097.
Proteomic databases
PaxDb	Q8K097.
PRIDE	Q8K097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023750; ENSMUSP00000023750; ENSMUSG00000023011.
GeneID	72393.
KEGG	mmu:72393.
UCSC	uc007xpp.1. mouse. uc007xpq.1. mouse.
Organism-specific databases
CTD	23017.
MGI	MGI:1919643. Faim2.
Rouge	Search...
Phylogenomic databases
eggNOG	COG0670.
GeneTree	ENSGT00500000044791.
HOGENOM	HOG000060393.
HOVERGEN	HBG012084.
InParanoid	Q8K097.
OMA	MGNRRYS.
OrthoDB	EOG43R3N3.
Gene expression databases
Bgee	Q8K097.
CleanEx	MM_FAIM2.
Genevestigator	Q8K097.
GermOnline	ENSMUSG00000023011. Mus musculus.
Family and domain databases
InterPro	IPR006214. Bax_inhibitor_1-related. [Graphical view]
PANTHER	PTHR23291. PTHR23291. 1 hit.
Pfam	PF01027. Bax1-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	336176.
SOURCE	Search...

Entry information

Entry name

LFG2_MOUSE

Accession

Primary (citable) accession number: Q8K097
Secondary accession number(s): Q3TY22, Q8K1F6, Q9D6K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program