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Q8K097 (LFG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein lifeguard 2
Alternative name(s):
Fas apoptotic inhibitory molecule 2
Neural membrane protein 35
Gene names
Name:Faim2
Synonyms:Kiaa0950, Lfg, Lfg2, Nmp35
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. Plays a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development. Ref.5 Ref.7 Ref.8

Subunit structure

Interacts with FAS/TNFRSF6 and BAX By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Membrane raft. Cell junctionsynapsepostsynaptic cell membrane By similarity Ref.5.

Tissue specificity

Brain. Highly expressed in cerebellum, also found in cortex, olfactory bulb, and hippocampus. Ref.5 Ref.8

Disruption phenotype

Mice show reduced cerebellar size and internal granular layer (IGL) thickness in early developmental stages, delayed Purkinje Cell (PC) development, with an abnormal morphology and reduced cellular density, increased caspase-8 and caspase-3 activity in PCs and higher sensitivity to Fas-mediated apoptosis. Ref.8

Sequence similarities

Belongs to the BI1 family. LFG subfamily.

Sequence caution

The sequence BAC98056.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K097-1)

Also known as: Long; LFG-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K097-2)

Also known as: Short; LFG-S;

The sequence of this isoform differs from the canonical sequence as follows:
     72-84: SGSSGYEGGFPAG → R
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Protein lifeguard 2
PRO_0000179088

Regions

Transmembrane107 – 12721Helical; Potential
Transmembrane139 – 15921Helical; Potential
Transmembrane166 – 18621Helical; Potential
Transmembrane195 – 21521Helical; Potential
Transmembrane226 – 24621Helical; Potential
Transmembrane252 – 27221Helical; Potential
Transmembrane291 – 31121Helical; Potential

Amino acid modifications

Glycosylation1921N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence72 – 8413SGSSG…GFPAG → R in isoform 2.
VSP_008994

Experimental info

Sequence conflict2561A → T in BAC98056. Ref.2
Sequence conflict3121F → Y in AAM46781. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (LFG-L) [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: A62D51BA49BCDB15

FASTA31735,258
        10         20         30         40         50         60 
MTQGKLSVAN KAPGTEGQQH QANGEKKDAP AVPSAPPSYE EATSGEGLKA GTFPQGPTAV 

        70         80         90        100        110        120 
PLHPSWAYVD PSGSSGYEGG FPAGHHEHFT TFSWDDQKVR RLFIRKVYTI LLVQLLVTLA 

       130        140        150        160        170        180 
VVALFTFCDV VKDYVQANPG WYWASYAVFF ATYLTLACCS GPRRHFPWNL ILLTIFTLSM 

       190        200        210        220        230        240 
AYLTGMLSSY YNTTSVLLCL VITALVCLSV TIFSFQTKFD FTSCQGVLFV LLMTLFFSGL 

       250        260        270        280        290        300 
LLAVLLPFQY VPWLHAVYAV LGAGVFTLFL AFDTQLLMGN RRHSLSPEEY IFGALNIYLD 

       310 
IIYIFTFFLQ LFGTNRE 

« Hide

Isoform 2 (Short) (LFG-S) [UniParc].

Checksum: 8793FA6D2464F6B8
Show »

FASTA30534,260

References

« Hide 'large scale' references
[1]"Cloning and characterization of the long form of mouse lifeguard (LFG-L), an anti Fas-apoptotic molecule."
Sole C., Segura M.F., Bayascas J.R., Comella J.X.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Retina.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Hippocampus and Visual cortex.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Retina.
[5]"Lifeguard/neuronal membrane protein 35 regulates Fas ligand-mediated apoptosis in neurons via microdomain recruitment."
Fernandez M., Segura M.F., Sole C., Colino A., Comella J.X., Cena V.
J. Neurochem. 103:190-203(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"LFG: a candidate apoptosis regulatory gene family."
Hu L., Smith T.F., Goldberger G.
Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"Fas/CD95 regulatory protein Faim2 is neuroprotective after transient brain ischemia."
Reich A., Spering C., Gertz K., Harms C., Gerhardt E., Kronenberg G., Nave K.A., Schwab M., Tauber S.C., Drinkut A., Harms K., Beier C.P., Voigt A., Goebbels S., Endres M., Schulz J.B.
J. Neurosci. 31:225-233(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Antiapoptotic protein Lifeguard is required for survival and maintenance of Purkinje and granular cells."
Hurtado de Mendoza T., Perez-Garcia C.G., Kroll T.T., Hoong N.H., O'Leary D.D., Verma I.M.
Proc. Natl. Acad. Sci. U.S.A. 108:17189-17194(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF468028 mRNA. Translation: AAM46781.1.
AK013476 mRNA. Translation: BAB28874.1.
AK030513 mRNA. Translation: BAC26999.1.
AK129246 mRNA. Translation: BAC98056.1. Different initiation.
AK158960 mRNA. Translation: BAE34742.1.
BC032278 mRNA. Translation: AAH32278.1.
RefSeqNP_001033747.1. NM_001038658.2.
NP_082500.2. NM_028224.4.
XP_006521512.1. XM_006521449.1.
UniGeneMm.39760.

3D structure databases

ProteinModelPortalQ8K097.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K097.

Proteomic databases

PaxDbQ8K097.
PRIDEQ8K097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023750; ENSMUSP00000023750; ENSMUSG00000023011. [Q8K097-1]
GeneID72393.
KEGGmmu:72393.
UCSCuc007xpp.1. mouse. [Q8K097-2]
uc007xpq.1. mouse. [Q8K097-1]

Organism-specific databases

CTD23017.
MGIMGI:1919643. Faim2.
RougeSearch...

Phylogenomic databases

eggNOGCOG0670.
GeneTreeENSGT00500000044791.
HOGENOMHOG000060393.
HOVERGENHBG012084.
InParanoidQ8K097.
OMASCQGVIF.
OrthoDBEOG7VB2G8.
PhylomeDBQ8K097.
TreeFamTF319996.

Gene expression databases

BgeeQ8K097.
CleanExMM_FAIM2.
GenevestigatorQ8K097.

Family and domain databases

InterProIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERPTHR23291. PTHR23291. 1 hit.
PfamPF01027. Bax1-I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio336176.
PROQ8K097.
SOURCESearch...

Entry information

Entry nameLFG2_MOUSE
AccessionPrimary (citable) accession number: Q8K097
Secondary accession number(s): Q3TY22, Q8K1F6, Q9D6K4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot