Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8K097

- LFG2_MOUSE

UniProt

Q8K097 - LFG2_MOUSE

Protein

Protein lifeguard 2

Gene

Faim2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. Plays a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development.3 Publications

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cerebellar granular layer development Source: UniProtKB
    3. cerebellar Purkinje cell differentiation Source: UniProtKB
    4. cerebellar Purkinje cell layer development Source: UniProtKB
    5. cerebellum development Source: UniProtKB
    6. negative regulation of apoptotic signaling pathway Source: MGI
    7. negative regulation of neuron apoptotic process Source: MGI
    8. regulation of neuron apoptotic process Source: UniProtKB
    9. response to ischemia Source: MGI

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein lifeguard 2
    Alternative name(s):
    Fas apoptotic inhibitory molecule 2
    Neural membrane protein 35
    Gene namesi
    Name:Faim2
    Synonyms:Kiaa0950, Lfg, Lfg2, Nmp35
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1919643. Faim2.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane raft 1 Publication. Cell junctionsynapsepostsynaptic cell membrane By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane raft Source: UniProtKB
    4. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Disruption phenotypei

    Mice show reduced cerebellar size and internal granular layer (IGL) thickness in early developmental stages, delayed Purkinje Cell (PC) development, with an abnormal morphology and reduced cellular density, increased caspase-8 and caspase-3 activity in PCs and higher sensitivity to Fas-mediated apoptosis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Protein lifeguard 2PRO_0000179088Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8K097.
    PRIDEiQ8K097.

    PTM databases

    PhosphoSiteiQ8K097.

    Expressioni

    Tissue specificityi

    Brain. Highly expressed in cerebellum, also found in cortex, olfactory bulb, and hippocampus.2 Publications

    Gene expression databases

    BgeeiQ8K097.
    CleanExiMM_FAIM2.
    GenevestigatoriQ8K097.

    Interactioni

    Subunit structurei

    Interacts with FAS/TNFRSF6 and BAX.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K097.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei107 – 12721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei139 – 15921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei166 – 18621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei195 – 21521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei226 – 24621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei252 – 27221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei291 – 31121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the BI1 family. LFG subfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0670.
    GeneTreeiENSGT00500000044791.
    HOGENOMiHOG000060393.
    HOVERGENiHBG012084.
    InParanoidiQ8K097.
    OMAiSCQGVIF.
    OrthoDBiEOG7VB2G8.
    PhylomeDBiQ8K097.
    TreeFamiTF319996.

    Family and domain databases

    InterProiIPR006214. Bax_inhibitor_1-related.
    [Graphical view]
    PANTHERiPTHR23291. PTHR23291. 1 hit.
    PfamiPF01027. Bax1-I. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K097-1) [UniParc]FASTAAdd to Basket

    Also known as: Long, LFG-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTQGKLSVAN KAPGTEGQQH QANGEKKDAP AVPSAPPSYE EATSGEGLKA    50
    GTFPQGPTAV PLHPSWAYVD PSGSSGYEGG FPAGHHEHFT TFSWDDQKVR 100
    RLFIRKVYTI LLVQLLVTLA VVALFTFCDV VKDYVQANPG WYWASYAVFF 150
    ATYLTLACCS GPRRHFPWNL ILLTIFTLSM AYLTGMLSSY YNTTSVLLCL 200
    VITALVCLSV TIFSFQTKFD FTSCQGVLFV LLMTLFFSGL LLAVLLPFQY 250
    VPWLHAVYAV LGAGVFTLFL AFDTQLLMGN RRHSLSPEEY IFGALNIYLD 300
    IIYIFTFFLQ LFGTNRE 317
    Length:317
    Mass (Da):35,258
    Last modified:October 1, 2002 - v1
    Checksum:iA62D51BA49BCDB15
    GO
    Isoform 2 (identifier: Q8K097-2) [UniParc]FASTAAdd to Basket

    Also known as: Short, LFG-S

    The sequence of this isoform differs from the canonical sequence as follows:
         72-84: SGSSGYEGGFPAG → R

    Note: No experimental confirmation available.

    Show »
    Length:305
    Mass (Da):34,260
    Checksum:i8793FA6D2464F6B8
    GO

    Sequence cautioni

    The sequence BAC98056.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti256 – 2561A → T in BAC98056. (PubMed:12693553)Curated
    Sequence conflicti312 – 3121F → Y in AAM46781. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei72 – 8413SGSSG…GFPAG → R in isoform 2. 1 PublicationVSP_008994Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF468028 mRNA. Translation: AAM46781.1.
    AK013476 mRNA. Translation: BAB28874.1.
    AK030513 mRNA. Translation: BAC26999.1.
    AK129246 mRNA. Translation: BAC98056.1. Different initiation.
    AK158960 mRNA. Translation: BAE34742.1.
    BC032278 mRNA. Translation: AAH32278.1.
    CCDSiCCDS37203.1. [Q8K097-1]
    RefSeqiNP_001033747.1. NM_001038658.2. [Q8K097-2]
    NP_082500.2. NM_028224.4. [Q8K097-1]
    XP_006521512.1. XM_006521449.1. [Q8K097-1]
    UniGeneiMm.39760.

    Genome annotation databases

    EnsembliENSMUST00000023750; ENSMUSP00000023750; ENSMUSG00000023011. [Q8K097-1]
    GeneIDi72393.
    KEGGimmu:72393.
    UCSCiuc007xpp.1. mouse. [Q8K097-2]
    uc007xpq.1. mouse. [Q8K097-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF468028 mRNA. Translation: AAM46781.1 .
    AK013476 mRNA. Translation: BAB28874.1 .
    AK030513 mRNA. Translation: BAC26999.1 .
    AK129246 mRNA. Translation: BAC98056.1 . Different initiation.
    AK158960 mRNA. Translation: BAE34742.1 .
    BC032278 mRNA. Translation: AAH32278.1 .
    CCDSi CCDS37203.1. [Q8K097-1 ]
    RefSeqi NP_001033747.1. NM_001038658.2. [Q8K097-2 ]
    NP_082500.2. NM_028224.4. [Q8K097-1 ]
    XP_006521512.1. XM_006521449.1. [Q8K097-1 ]
    UniGenei Mm.39760.

    3D structure databases

    ProteinModelPortali Q8K097.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K097.

    Proteomic databases

    PaxDbi Q8K097.
    PRIDEi Q8K097.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023750 ; ENSMUSP00000023750 ; ENSMUSG00000023011 . [Q8K097-1 ]
    GeneIDi 72393.
    KEGGi mmu:72393.
    UCSCi uc007xpp.1. mouse. [Q8K097-2 ]
    uc007xpq.1. mouse. [Q8K097-1 ]

    Organism-specific databases

    CTDi 23017.
    MGIi MGI:1919643. Faim2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0670.
    GeneTreei ENSGT00500000044791.
    HOGENOMi HOG000060393.
    HOVERGENi HBG012084.
    InParanoidi Q8K097.
    OMAi SCQGVIF.
    OrthoDBi EOG7VB2G8.
    PhylomeDBi Q8K097.
    TreeFami TF319996.

    Miscellaneous databases

    NextBioi 336176.
    PROi Q8K097.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K097.
    CleanExi MM_FAIM2.
    Genevestigatori Q8K097.

    Family and domain databases

    InterProi IPR006214. Bax_inhibitor_1-related.
    [Graphical view ]
    PANTHERi PTHR23291. PTHR23291. 1 hit.
    Pfami PF01027. Bax1-I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the long form of mouse lifeguard (LFG-L), an anti Fas-apoptotic molecule."
      Sole C., Segura M.F., Bayascas J.R., Comella J.X.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Retina.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Hippocampus and Visual cortex.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Retina.
    5. "Lifeguard/neuronal membrane protein 35 regulates Fas ligand-mediated apoptosis in neurons via microdomain recruitment."
      Fernandez M., Segura M.F., Sole C., Colino A., Comella J.X., Cena V.
      J. Neurochem. 103:190-203(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "LFG: a candidate apoptosis regulatory gene family."
      Hu L., Smith T.F., Goldberger G.
      Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. Cited for: FUNCTION.
    8. "Antiapoptotic protein Lifeguard is required for survival and maintenance of Purkinje and granular cells."
      Hurtado de Mendoza T., Perez-Garcia C.G., Kroll T.T., Hoong N.H., O'Leary D.D., Verma I.M.
      Proc. Natl. Acad. Sci. U.S.A. 108:17189-17194(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLFG2_MOUSE
    AccessioniPrimary (citable) accession number: Q8K097
    Secondary accession number(s): Q3TY22, Q8K1F6, Q9D6K4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 21, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3