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Q8K097

- LFG2_MOUSE

UniProt

Q8K097 - LFG2_MOUSE

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Protein

Protein lifeguard 2

Gene

Faim2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. Plays a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development.3 Publications

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cerebellar granular layer development Source: UniProtKB
  3. cerebellar Purkinje cell differentiation Source: UniProtKB
  4. cerebellar Purkinje cell layer development Source: UniProtKB
  5. cerebellum development Source: UniProtKB
  6. negative regulation of apoptotic signaling pathway Source: MGI
  7. negative regulation of neuron apoptotic process Source: MGI
  8. regulation of neuron apoptotic process Source: UniProtKB
  9. response to ischemia Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lifeguard 2
Alternative name(s):
Fas apoptotic inhibitory molecule 2
Neural membrane protein 35
Gene namesi
Name:Faim2
Synonyms:Kiaa0950, Lfg, Lfg2, Nmp35
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1919643. Faim2.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane raft 1 Publication. Cell junctionsynapsepostsynaptic cell membrane By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei107 – 12721HelicalSequence AnalysisAdd
BLAST
Transmembranei139 – 15921HelicalSequence AnalysisAdd
BLAST
Transmembranei166 – 18621HelicalSequence AnalysisAdd
BLAST
Transmembranei195 – 21521HelicalSequence AnalysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence AnalysisAdd
BLAST
Transmembranei252 – 27221HelicalSequence AnalysisAdd
BLAST
Transmembranei291 – 31121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane raft Source: UniProtKB
  4. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice show reduced cerebellar size and internal granular layer (IGL) thickness in early developmental stages, delayed Purkinje Cell (PC) development, with an abnormal morphology and reduced cellular density, increased caspase-8 and caspase-3 activity in PCs and higher sensitivity to Fas-mediated apoptosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Protein lifeguard 2PRO_0000179088Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8K097.
PRIDEiQ8K097.

PTM databases

PhosphoSiteiQ8K097.

Expressioni

Tissue specificityi

Brain. Highly expressed in cerebellum, also found in cortex, olfactory bulb, and hippocampus.2 Publications

Gene expression databases

BgeeiQ8K097.
CleanExiMM_FAIM2.
GenevestigatoriQ8K097.

Interactioni

Subunit structurei

Interacts with FAS/TNFRSF6 and BAX.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8K097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BI1 family. LFG subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0670.
GeneTreeiENSGT00500000044791.
HOGENOMiHOG000060393.
HOVERGENiHBG012084.
InParanoidiQ8K097.
OMAiSCQGVIF.
OrthoDBiEOG7VB2G8.
PhylomeDBiQ8K097.
TreeFamiTF319996.

Family and domain databases

InterProiIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 1 hit.
PfamiPF01027. Bax1-I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K097-1) [UniParc]FASTAAdd to Basket

Also known as: Long, LFG-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTQGKLSVAN KAPGTEGQQH QANGEKKDAP AVPSAPPSYE EATSGEGLKA
60 70 80 90 100
GTFPQGPTAV PLHPSWAYVD PSGSSGYEGG FPAGHHEHFT TFSWDDQKVR
110 120 130 140 150
RLFIRKVYTI LLVQLLVTLA VVALFTFCDV VKDYVQANPG WYWASYAVFF
160 170 180 190 200
ATYLTLACCS GPRRHFPWNL ILLTIFTLSM AYLTGMLSSY YNTTSVLLCL
210 220 230 240 250
VITALVCLSV TIFSFQTKFD FTSCQGVLFV LLMTLFFSGL LLAVLLPFQY
260 270 280 290 300
VPWLHAVYAV LGAGVFTLFL AFDTQLLMGN RRHSLSPEEY IFGALNIYLD
310
IIYIFTFFLQ LFGTNRE
Length:317
Mass (Da):35,258
Last modified:October 1, 2002 - v1
Checksum:iA62D51BA49BCDB15
GO
Isoform 2 (identifier: Q8K097-2) [UniParc]FASTAAdd to Basket

Also known as: Short, LFG-S

The sequence of this isoform differs from the canonical sequence as follows:
     72-84: SGSSGYEGGFPAG → R

Note: No experimental confirmation available.

Show »
Length:305
Mass (Da):34,260
Checksum:i8793FA6D2464F6B8
GO

Sequence cautioni

The sequence BAC98056.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2561A → T in BAC98056. (PubMed:12693553)Curated
Sequence conflicti312 – 3121F → Y in AAM46781. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei72 – 8413SGSSG…GFPAG → R in isoform 2. 1 PublicationVSP_008994Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF468028 mRNA. Translation: AAM46781.1.
AK013476 mRNA. Translation: BAB28874.1.
AK030513 mRNA. Translation: BAC26999.1.
AK129246 mRNA. Translation: BAC98056.1. Different initiation.
AK158960 mRNA. Translation: BAE34742.1.
BC032278 mRNA. Translation: AAH32278.1.
CCDSiCCDS37203.1. [Q8K097-1]
RefSeqiNP_001033747.1. NM_001038658.2. [Q8K097-2]
NP_082500.2. NM_028224.4. [Q8K097-1]
XP_006521512.1. XM_006521449.1. [Q8K097-1]
UniGeneiMm.39760.

Genome annotation databases

EnsembliENSMUST00000023750; ENSMUSP00000023750; ENSMUSG00000023011. [Q8K097-1]
GeneIDi72393.
KEGGimmu:72393.
UCSCiuc007xpp.1. mouse. [Q8K097-2]
uc007xpq.1. mouse. [Q8K097-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF468028 mRNA. Translation: AAM46781.1 .
AK013476 mRNA. Translation: BAB28874.1 .
AK030513 mRNA. Translation: BAC26999.1 .
AK129246 mRNA. Translation: BAC98056.1 . Different initiation.
AK158960 mRNA. Translation: BAE34742.1 .
BC032278 mRNA. Translation: AAH32278.1 .
CCDSi CCDS37203.1. [Q8K097-1 ]
RefSeqi NP_001033747.1. NM_001038658.2. [Q8K097-2 ]
NP_082500.2. NM_028224.4. [Q8K097-1 ]
XP_006521512.1. XM_006521449.1. [Q8K097-1 ]
UniGenei Mm.39760.

3D structure databases

ProteinModelPortali Q8K097.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8K097.

Proteomic databases

PaxDbi Q8K097.
PRIDEi Q8K097.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023750 ; ENSMUSP00000023750 ; ENSMUSG00000023011 . [Q8K097-1 ]
GeneIDi 72393.
KEGGi mmu:72393.
UCSCi uc007xpp.1. mouse. [Q8K097-2 ]
uc007xpq.1. mouse. [Q8K097-1 ]

Organism-specific databases

CTDi 23017.
MGIi MGI:1919643. Faim2.
Rougei Search...

Phylogenomic databases

eggNOGi COG0670.
GeneTreei ENSGT00500000044791.
HOGENOMi HOG000060393.
HOVERGENi HBG012084.
InParanoidi Q8K097.
OMAi SCQGVIF.
OrthoDBi EOG7VB2G8.
PhylomeDBi Q8K097.
TreeFami TF319996.

Miscellaneous databases

NextBioi 336176.
PROi Q8K097.
SOURCEi Search...

Gene expression databases

Bgeei Q8K097.
CleanExi MM_FAIM2.
Genevestigatori Q8K097.

Family and domain databases

InterProi IPR006214. Bax_inhibitor_1-related.
[Graphical view ]
PANTHERi PTHR23291. PTHR23291. 1 hit.
Pfami PF01027. Bax1-I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the long form of mouse lifeguard (LFG-L), an anti Fas-apoptotic molecule."
    Sole C., Segura M.F., Bayascas J.R., Comella J.X.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Retina.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Hippocampus and Visual cortex.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Retina.
  5. "Lifeguard/neuronal membrane protein 35 regulates Fas ligand-mediated apoptosis in neurons via microdomain recruitment."
    Fernandez M., Segura M.F., Sole C., Colino A., Comella J.X., Cena V.
    J. Neurochem. 103:190-203(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "LFG: a candidate apoptosis regulatory gene family."
    Hu L., Smith T.F., Goldberger G.
    Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. Cited for: FUNCTION.
  8. "Antiapoptotic protein Lifeguard is required for survival and maintenance of Purkinje and granular cells."
    Hurtado de Mendoza T., Perez-Garcia C.G., Kroll T.T., Hoong N.H., O'Leary D.D., Verma I.M.
    Proc. Natl. Acad. Sci. U.S.A. 108:17189-17194(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLFG2_MOUSE
AccessioniPrimary (citable) accession number: Q8K097
Secondary accession number(s): Q3TY22, Q8K1F6, Q9D6K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3