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Protein

Thyrotropin-releasing hormone-degrading ectoenzyme

Gene

Trhde

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specific inactivation of TRH after its release.By similarity

Catalytic activityi

Release of the N-terminal pyroglutamyl group from pGlu-|-His-Xaa tripeptides and pGlu-|-His-Xaa-Gly tetrapeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi441 – 4411Zinc; catalyticPROSITE-ProRule annotation
Active sitei442 – 4421Proton acceptorPROSITE-ProRule annotation
Metal bindingi445 – 4451Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi464 – 4641Zinc; catalyticPROSITE-ProRule annotation
Sitei528 – 5281Transition state stabilizerBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotropin-releasing hormone-degrading ectoenzyme (EC:3.4.19.6)
Short name:
TRH-DE
Short name:
TRH-degrading ectoenzyme
Alternative name(s):
Pyroglutamyl-peptidase II
Short name:
PAP-II
TRH-specific aminopeptidase
Thyroliberinase
Gene namesi
Name:Trhde
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2384311. Trhde.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4040CytoplasmicSequence analysisAdd
BLAST
Transmembranei41 – 6121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini62 – 1025964ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Thyrotropin-releasing hormone-degrading ectoenzymePRO_0000095119Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphothreonine; by PKCSequence analysis
Disulfide bondi68 – 68InterchainBy similarity
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence analysis
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence analysis
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence analysis
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence analysis
Glycosylationi339 – 3391N-linked (GlcNAc...)Sequence analysis
Glycosylationi606 – 6061N-linked (GlcNAc...)Sequence analysis
Glycosylationi635 – 6351N-linked (GlcNAc...)Sequence analysis
Glycosylationi650 – 6501N-linked (GlcNAc...)Sequence analysis
Glycosylationi664 – 6641N-linked (GlcNAc...)Sequence analysis
Glycosylationi685 – 6851N-linked (GlcNAc...)Sequence analysis
Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence analysis
Glycosylationi907 – 9071N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8K093.
PaxDbiQ8K093.
PRIDEiQ8K093.

PTM databases

PhosphoSiteiQ8K093.

Expressioni

Gene expression databases

BgeeiQ8K093.
CleanExiMM_TRHDE.
GenevisibleiQ8K093. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057449.

Structurei

3D structure databases

ProteinModelPortaliQ8K093.
SMRiQ8K093. Positions 136-1019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni405 – 4095Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
HOVERGENiHBG095698.
InParanoidiQ8K093.
KOiK01306.
OMAiKYNVFNE.
OrthoDBiEOG754HNR.
PhylomeDBiQ8K093.
TreeFamiTF300395.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR015570. TRH-DE.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 2 hits.
PTHR11533:SF40. PTHR11533:SF40. 2 hits.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEDDAALRA SGRGLSDPWA DSVGVRPRTT ERHIAVHKRL VLAFAVSIVA
60 70 80 90 100
LLAVTMLAVL LSLRFDECGA SAAMPGTDGG LGGFPERDSN SSFPGSARRN
110 120 130 140 150
HHAGGESSQR ESGEVGTPGT PSAQPPSEEE REQWQPWTQL RLSGHLKPLH
160 170 180 190 200
YNLMLTAFME NFTFSGEVNV EIACRNATRY VVLHASRVAV EKVQVAEDRA
210 220 230 240 250
FGAVPVAGFF LYPQTQVLVV VLNRTLDAQR HYNLKIIYNA LIENELLGFF
260 270 280 290 300
RSSYVIHGER RFLGVTQFSP THARKAFPCF DEPIYKATFK ISIKHQATYL
310 320 330 340 350
SLSNMPVETS VFEEDGWVTD HFSQTPLMST YYLAWAICNF TYRETTTKSG
360 370 380 390 400
VVVRLYARPD AIRRGSGDYA LHITKRLIEF YEDYFKVPYS LPKLDLLAVP
410 420 430 440 450
KHPYAAMENW GLSIFVEQRI LLDPSVSSIS YLLDVTMVIV HEICHQWFGD
460 470 480 490 500
LVTPVWWEDV WLKEGFAHYF EFVGTDYLYP AWNMEKQRFL TDVLHEVMLL
510 520 530 540 550
DGLASSHPVS QEVLRATDID RVFDWIAYKK GAALIRMLAN FMGHSVFQRG
560 570 580 590 600
LQDYLTIHKY GNAARNDLWN TLSEALRRNG KYVNIQEVMD QWTLQMGYPV
610 620 630 640 650
ITILGNTTAE NRILITQQHF IYDIGAKTKA LQLQNSSYLW QIPLTIVVGN
660 670 680 690 700
RSHVSSEAII WVSNKSEHHR IAYLDRGSWI LGNINQTGYF RVNYDLRNWR
710 720 730 740 750
LLIDQLIRNH EVLSVSNRAA LIDDAFSLAR AGYLPQNIPL EIIRYLSEEK
760 770 780 790 800
DFLPWHAASR ALYPLDKLLD RMENYNIFNE YILKQVATTY IKLGWPRNNF
810 820 830 840 850
NGSLVQASYQ HEELRREVIM LACSFGNKHC HQQASTLISD WISSNRNRIP
860 870 880 890 900
LNVRDIVYCT GVSLLDEDVW EFIWMKFHST TAVSEKKILL EALTCSDDRN
910 920 930 940 950
LLSRLLNLSL NSEVVLDQDA IDVIIHVARN PHGRDLAWKF FRDKWKILNT
960 970 980 990 1000
RYGEALFMNS KLISGVTEFL NTEGELKELK NFMKSYDGVA SASFSRAVET
1010 1020
VEANVRWKRF YQDELFQWLG KAMRH
Length:1,025
Mass (Da):117,458
Last modified:October 1, 2002 - v1
Checksum:i0990B2A78A13B8AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032288 mRNA. Translation: AAH32288.1.
CCDSiCCDS24175.1.
RefSeqiNP_666353.1. NM_146241.2.
UniGeneiMm.445550.

Genome annotation databases

EnsembliENSMUST00000061632; ENSMUSP00000057449; ENSMUSG00000050663.
GeneIDi237553.
KEGGimmu:237553.
UCSCiuc007hat.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032288 mRNA. Translation: AAH32288.1.
CCDSiCCDS24175.1.
RefSeqiNP_666353.1. NM_146241.2.
UniGeneiMm.445550.

3D structure databases

ProteinModelPortaliQ8K093.
SMRiQ8K093. Positions 136-1019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057449.

Protein family/group databases

MEROPSiM01.008.

PTM databases

PhosphoSiteiQ8K093.

Proteomic databases

MaxQBiQ8K093.
PaxDbiQ8K093.
PRIDEiQ8K093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061632; ENSMUSP00000057449; ENSMUSG00000050663.
GeneIDi237553.
KEGGimmu:237553.
UCSCiuc007hat.2. mouse.

Organism-specific databases

CTDi29953.
MGIiMGI:2384311. Trhde.

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
HOVERGENiHBG095698.
InParanoidiQ8K093.
KOiK01306.
OMAiKYNVFNE.
OrthoDBiEOG754HNR.
PhylomeDBiQ8K093.
TreeFamiTF300395.

Miscellaneous databases

ChiTaRSiTrhde. mouse.
NextBioi383404.
PROiQ8K093.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K093.
CleanExiMM_TRHDE.
GenevisibleiQ8K093. MM.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR015570. TRH-DE.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 2 hits.
PTHR11533:SF40. PTHR11533:SF40. 2 hits.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiTRHDE_MOUSE
AccessioniPrimary (citable) accession number: Q8K093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.