ID   IFT80_MOUSE             Reviewed;         777 AA.
AC   Q8K057; Q6ZPT0; Q8C9F1; Q91YV4;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   24-JAN-2024, entry version 149.
DE   RecName: Full=Intraflagellar transport protein 80 homolog;
DE   AltName: Full=WD repeat-containing protein 56;
GN   Name=Ift80; Synonyms=Kiaa1374, Wdr56;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Embryonic tail;
RX   PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA   Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA   Saga Y., Nagase T., Ohara O., Koga H.;
RT   "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT   The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT   identified by screening of terminal sequences of cDNA clones randomly
RT   sampled from size-fractionated libraries.";
RL   DNA Res. 10:167-180(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Eye, and Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-751 (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Thymus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=17468754; DOI=10.1038/ng2038;
RA   Beales P.L., Bland E., Tobin J.L., Bacchelli C., Tuysuz B., Hill J.,
RA   Rix S., Pearson C.G., Kai M., Hartley J., Johnson C., Irving M.,
RA   Elcioglu N., Winey M., Tada M., Scambler P.J.;
RT   "IFT80, which encodes a conserved intraflagellar transport protein, is
RT   mutated in Jeune asphyxiating thoracic dystrophy.";
RL   Nat. Genet. 39:727-729(2007).
RN   [5]
RP   IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH IFT88, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=19253336; DOI=10.1002/cm.20346;
RA   Follit J.A., Xu F., Keady B.T., Pazour G.J.;
RT   "Characterization of mouse IFT complex B.";
RL   Cell Motil. Cytoskeleton 66:457-468(2009).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [7]
RP   IDENTIFICATION IN THE IFT COMPLEX B, AND INTERACTION WITH IFT57 AND IFT70B.
RX   PubMed=23810713; DOI=10.1016/j.yexcr.2013.06.010;
RA   Howard P.W., Jue S.F., Maurer R.A.;
RT   "Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport
RT   complex B proteins and KIF17.";
RL   Exp. Cell Res. 319:2275-2281(2013).
CC   -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B,
CC       which is essential for the development and maintenance of motile and
CC       sensory cilia. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC       IFT22, IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80,
CC       IFT81, and IFT88 (PubMed:19253336, PubMed:23810713). Interacts with
CC       IFT88 (PubMed:19253336). Interacts with IFT57 and IFT70B
CC       (PubMed:23810713). {ECO:0000269|PubMed:19253336,
CC       ECO:0000269|PubMed:23810713}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton, cilium basal
CC       body. Cytoplasm, cytoskeleton, cilium axoneme. Note=Basal body and
CC       ciliary axoneme in the chondrocytic ATDC-5 cell line.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8K057-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8K057-2; Sequence=VSP_027991, VSP_027992;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC98149.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AK129339; BAC98149.1; ALT_INIT; mRNA.
DR   EMBL; BC013814; AAH13814.1; -; mRNA.
DR   EMBL; BC034101; AAH34101.1; -; mRNA.
DR   EMBL; AK042231; BAC31200.1; -; mRNA.
DR   CCDS; CCDS38451.1; -. [Q8K057-1]
DR   RefSeq; NP_080917.1; NM_026641.2. [Q8K057-1]
DR   RefSeq; XP_006502029.1; XM_006501966.3. [Q8K057-1]
DR   AlphaFoldDB; Q8K057; -.
DR   SMR; Q8K057; -.
DR   BioGRID; 212765; 5.
DR   ComplexPortal; CPX-5028; Intraflagellar transport complex B.
DR   STRING; 10090.ENSMUSP00000103442; -.
DR   iPTMnet; Q8K057; -.
DR   PhosphoSitePlus; Q8K057; -.
DR   SwissPalm; Q8K057; -.
DR   EPD; Q8K057; -.
DR   MaxQB; Q8K057; -.
DR   PaxDb; 10090-ENSMUSP00000133263; -.
DR   ProteomicsDB; 267214; -. [Q8K057-1]
DR   ProteomicsDB; 267215; -. [Q8K057-2]
DR   Pumba; Q8K057; -.
DR   Antibodypedia; 50241; 79 antibodies from 17 providers.
DR   DNASU; 68259; -.
DR   Ensembl; ENSMUST00000029347.14; ENSMUSP00000029347.8; ENSMUSG00000027778.16. [Q8K057-1]
DR   Ensembl; ENSMUST00000107812.8; ENSMUSP00000103442.2; ENSMUSG00000027778.16. [Q8K057-1]
DR   Ensembl; ENSMUST00000169064.8; ENSMUSP00000133263.2; ENSMUSG00000027778.16. [Q8K057-1]
DR   GeneID; 68259; -.
DR   KEGG; mmu:68259; -.
DR   UCSC; uc033hub.1; mouse. [Q8K057-1]
DR   AGR; MGI:1915509; -.
DR   CTD; 57560; -.
DR   MGI; MGI:1915509; Ift80.
DR   VEuPathDB; HostDB:ENSMUSG00000027778; -.
DR   eggNOG; KOG1524; Eukaryota.
DR   GeneTree; ENSGT00440000033499; -.
DR   HOGENOM; CLU_024638_1_0_1; -.
DR   InParanoid; Q8K057; -.
DR   OMA; WDAQGAN; -.
DR   OrthoDB; 5479095at2759; -.
DR   PhylomeDB; Q8K057; -.
DR   TreeFam; TF106117; -.
DR   Reactome; R-MMU-5620924; Intraflagellar transport.
DR   BioGRID-ORCS; 68259; 5 hits in 76 CRISPR screens.
DR   ChiTaRS; Ift80; mouse.
DR   PRO; PR:Q8K057; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q8K057; Protein.
DR   Bgee; ENSMUSG00000027778; Expressed in dorsal pancreas and 247 other cell types or tissues.
DR   ExpressionAtlas; Q8K057; baseline and differential.
DR   GO; GO:0097731; C:9+0 non-motile cilium; IDA:MGI.
DR   GO; GO:0005813; C:centrosome; IDA:MGI.
DR   GO; GO:0036064; C:ciliary basal body; IDA:MGI.
DR   GO; GO:0005929; C:cilium; IDA:MGI.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030992; C:intraciliary transport particle B; IDA:UniProtKB.
DR   GO; GO:0061975; P:articular cartilage development; IMP:MGI.
DR   GO; GO:0035630; P:bone mineralization involved in bone maturation; IMP:MGI.
DR   GO; GO:0060349; P:bone morphogenesis; IMP:MGI.
DR   GO; GO:0002062; P:chondrocyte differentiation; IMP:MGI.
DR   GO; GO:0060271; P:cilium assembly; IMP:MGI.
DR   GO; GO:0001958; P:endochondral ossification; IMP:MGI.
DR   GO; GO:0003418; P:growth plate cartilage chondrocyte differentiation; IMP:MGI.
DR   GO; GO:0003417; P:growth plate cartilage development; IMP:MGI.
DR   GO; GO:0035720; P:intraciliary anterograde transport; NAS:ComplexPortal.
DR   GO; GO:0042073; P:intraciliary transport; IC:MGI.
DR   GO; GO:0043616; P:keratinocyte proliferation; IMP:MGI.
DR   GO; GO:0060173; P:limb development; IMP:MGI.
DR   GO; GO:0010839; P:negative regulation of keratinocyte proliferation; IMP:MGI.
DR   GO; GO:2000051; P:negative regulation of non-canonical Wnt signaling pathway; IGI:MGI.
DR   GO; GO:0035567; P:non-canonical Wnt signaling pathway; IGI:MGI.
DR   GO; GO:1905515; P:non-motile cilium assembly; IMP:MGI.
DR   GO; GO:0001649; P:osteoblast differentiation; IMP:MGI.
DR   GO; GO:0033687; P:osteoblast proliferation; IMP:MGI.
DR   GO; GO:0097500; P:receptor localization to non-motile cilium; IMP:MGI.
DR   GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR   GO; GO:0007224; P:smoothened signaling pathway; IMP:MGI.
DR   Gene3D; 1.25.40.470; -; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR24098:SF11; INTRAFLAGELLAR TRANSPORT PROTEIN 80 HOMOLOG; 1.
DR   PANTHER; PTHR24098; OUTER SEGMENT 5; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50998; Quinoprotein alcohol dehydrogenase-like; 1.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
DR   Genevisible; Q8K057; MM.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW   Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..777
FT                   /note="Intraflagellar transport protein 80 homolog"
FT                   /id="PRO_0000051043"
FT   REPEAT          12..50
FT                   /note="WD 1"
FT   REPEAT          104..143
FT                   /note="WD 2"
FT   REPEAT          145..185
FT                   /note="WD 3"
FT   REPEAT          186..225
FT                   /note="WD 4"
FT   REPEAT          227..265
FT                   /note="WD 5"
FT   REPEAT          267..306
FT                   /note="WD 6"
FT   REPEAT          504..542
FT                   /note="WD 7"
FT   VAR_SEQ         439
FT                   /note="I -> K (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14621295"
FT                   /id="VSP_027991"
FT   VAR_SEQ         440
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14621295"
FT                   /id="VSP_027992"
SQ   SEQUENCE   777 AA;  87811 MW;  2B01621A12E660C4 CRC64;
     MRLKISLSKE PKHQELVSCV GWTTAEELYS CSDDHQIVKW NLLTSETSLI VKLPDDIYPI
     DLHWFPKSLG IKKQTQAESF VLTSSDGKFH LISKLGRVEK SVEAHCGAVL AGRWNYEGTA
     LVTVGEDGQV KIWSKTGMLR STLAQQGTPV YSVAWGPDSE KVLYTAGKQL IIKPLQPNAK
     VLQWKAHDGI ILKVDWNSVN DLILSAGEDC KYKVWDSYGR VLYGSQPHEH PITSVAWAPD
     GELFAVGSFH TLRLCDKTGW SYALEKPNTG SIFNIAWSID GTQIAGACGN GHVVFAHVVE
     QRWEWKNFQV TLTKRRTMQV RNVLNDAVDL LEFRDRVIKA SLNHAHLVVS TSLQCYVFST
     KNWNTPLIFD LKEGTVSLIL QAERHFLLVD GGGIYLHSYE GRFISSPKFP GMRTDILNAQ
     TVSLSNDTIA IKDKADEKII FLFEASTGKP LGDGKLLSHK NEISEIALDQ KGLTNDRKIA
     FIDKNRDLYI TSVKRFGKEE QIIKLGTMVH TLAWCDTCNI LCGIQDTRFT VWYYPNTIYV
     DRDILPKTLY ERDASEYSKN PHIVSFVGNQ VTIRRADGSL VHISISPYPA ILHEYVSSSK
     WEEAVRLCRF VKEQSMWACL AAMAVANRDM VTAEIAYAAV GEIDKVRYIN AIKDLPSRES
     KMAHILMFSG NIQEAETVLL QAGLVYQAIQ ININLYNWER ALELAVKYKT HVDTVLAYRQ
     KFLDTFGKQE TNKRYLQYAE GLQIDWEKIK AKIEMEITKE RDRSSSGQSS KSVGLKH
//