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Q8K057 (IFT80_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intraflagellar transport protein 80 homolog
Alternative name(s):
WD repeat-containing protein 56
Gene names
Name:Ift80
Synonyms:Kiaa1374, Wdr56
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length777 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia By similarity.

Subunit structure

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT88. Ref.5

Subcellular location

Cytoplasm. Cytoplasmcytoskeletoncilium basal body. Cytoplasmcytoskeletoncilium axoneme. Note: Basal body and ciliary axoneme in the chondrocytic ATDC-5 cell line. Ref.4 Ref.5

Sequence similarities

Contains 7 WD repeats.

Sequence caution

The sequence BAC98149.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell projection
Cilium
Cytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
   DomainRepeat
WD repeat
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbone morphogenesis

Inferred from mutant phenotype PubMed 21227999. Source: MGI

chondrocyte differentiation

Inferred from mutant phenotype PubMed 23333501. Source: MGI

cilium assembly

Inferred from mutant phenotype PubMed 23333501. Source: MGI

cilium morphogenesis

Inferred from mutant phenotype PubMed 21703454PubMed 22771375. Source: MGI

intraciliary transport

Inferred by curator Ref.5. Source: MGI

negative regulation of epithelial cell proliferation

Inferred from mutant phenotype PubMed 21703454. Source: MGI

negative regulation of non-canonical Wnt signaling pathway

Inferred from genetic interaction PubMed 23333501. Source: MGI

osteoblast differentiation

Inferred from mutant phenotype PubMed 22771375. Source: MGI

positive regulation of smoothened signaling pathway

Inferred from mutant phenotype PubMed 23333501. Source: MGI

smoothened signaling pathway

Inferred from mutant phenotype PubMed 21227999. Source: MGI

   Cellular_componentcentrosome

Inferred from direct assay Ref.5. Source: MGI

ciliary basal body

Inferred from sequence orthology PubMed 22922713. Source: MGI

cilium

Inferred from direct assay Ref.5PubMed 23333501. Source: MGI

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

intraciliary transport particle B

Inferred from direct assay Ref.5PubMed 23810713. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K057-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K057-2)

The sequence of this isoform differs from the canonical sequence as follows:
     439-439: I → K
     440-440: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 777777Intraflagellar transport protein 80 homolog
PRO_0000051043

Regions

Repeat12 – 5039WD 1
Repeat104 – 14340WD 2
Repeat145 – 18541WD 3
Repeat186 – 22540WD 4
Repeat227 – 26539WD 5
Repeat267 – 30640WD 6
Repeat504 – 54239WD 7

Natural variations

Alternative sequence4391I → K in isoform 2.
VSP_027991
Alternative sequence4401Missing in isoform 2.
VSP_027992

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 2B01621A12E660C4

FASTA77787,811
        10         20         30         40         50         60 
MRLKISLSKE PKHQELVSCV GWTTAEELYS CSDDHQIVKW NLLTSETSLI VKLPDDIYPI 

        70         80         90        100        110        120 
DLHWFPKSLG IKKQTQAESF VLTSSDGKFH LISKLGRVEK SVEAHCGAVL AGRWNYEGTA 

       130        140        150        160        170        180 
LVTVGEDGQV KIWSKTGMLR STLAQQGTPV YSVAWGPDSE KVLYTAGKQL IIKPLQPNAK 

       190        200        210        220        230        240 
VLQWKAHDGI ILKVDWNSVN DLILSAGEDC KYKVWDSYGR VLYGSQPHEH PITSVAWAPD 

       250        260        270        280        290        300 
GELFAVGSFH TLRLCDKTGW SYALEKPNTG SIFNIAWSID GTQIAGACGN GHVVFAHVVE 

       310        320        330        340        350        360 
QRWEWKNFQV TLTKRRTMQV RNVLNDAVDL LEFRDRVIKA SLNHAHLVVS TSLQCYVFST 

       370        380        390        400        410        420 
KNWNTPLIFD LKEGTVSLIL QAERHFLLVD GGGIYLHSYE GRFISSPKFP GMRTDILNAQ 

       430        440        450        460        470        480 
TVSLSNDTIA IKDKADEKII FLFEASTGKP LGDGKLLSHK NEISEIALDQ KGLTNDRKIA 

       490        500        510        520        530        540 
FIDKNRDLYI TSVKRFGKEE QIIKLGTMVH TLAWCDTCNI LCGIQDTRFT VWYYPNTIYV 

       550        560        570        580        590        600 
DRDILPKTLY ERDASEYSKN PHIVSFVGNQ VTIRRADGSL VHISISPYPA ILHEYVSSSK 

       610        620        630        640        650        660 
WEEAVRLCRF VKEQSMWACL AAMAVANRDM VTAEIAYAAV GEIDKVRYIN AIKDLPSRES 

       670        680        690        700        710        720 
KMAHILMFSG NIQEAETVLL QAGLVYQAIQ ININLYNWER ALELAVKYKT HVDTVLAYRQ 

       730        740        750        760        770 
KFLDTFGKQE TNKRYLQYAE GLQIDWEKIK AKIEMEITKE RDRSSSGQSS KSVGLKH 

« Hide

Isoform 2 [UniParc].

Checksum: C87C1E4C6A91C300
Show »

FASTA77687,712

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Embryonic tail.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye and Mammary tumor.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-751 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Thymus.
[4]"IFT80, which encodes a conserved intraflagellar transport protein, is mutated in Jeune asphyxiating thoracic dystrophy."
Beales P.L., Bland E., Tobin J.L., Bacchelli C., Tuysuz B., Hill J., Rix S., Pearson C.G., Kai M., Hartley J., Johnson C., Irving M., Elcioglu N., Winey M., Tada M., Scambler P.J.
Nat. Genet. 39:727-729(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Characterization of mouse IFT complex B."
Follit J.A., Xu F., Keady B.T., Pazour G.J.
Cell Motil. Cytoskeleton 66:457-468(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH IFT88, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK129339 mRNA. Translation: BAC98149.1. Different initiation.
BC013814 mRNA. Translation: AAH13814.1.
BC034101 mRNA. Translation: AAH34101.1.
AK042231 mRNA. Translation: BAC31200.1.
CCDSCCDS38451.1. [Q8K057-1]
RefSeqNP_080917.1. NM_026641.2. [Q8K057-1]
XP_006502029.1. XM_006501966.1. [Q8K057-1]
UniGeneMm.389451.

3D structure databases

ProteinModelPortalQ8K057.
SMRQ8K057. Positions 25-293.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid212765. 1 interaction.
STRING10090.ENSMUSP00000029347.

PTM databases

PhosphoSiteQ8K057.

Proteomic databases

MaxQBQ8K057.
PaxDbQ8K057.
PRIDEQ8K057.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029347; ENSMUSP00000029347; ENSMUSG00000027778. [Q8K057-1]
ENSMUST00000107812; ENSMUSP00000103442; ENSMUSG00000027778. [Q8K057-1]
ENSMUST00000169064; ENSMUSP00000133263; ENSMUSG00000027778. [Q8K057-1]
GeneID68259.
KEGGmmu:68259.
UCSCuc008plx.2. mouse. [Q8K057-1]

Organism-specific databases

CTD57560.
MGIMGI:1915509. Ift80.
RougeSearch...

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00440000033499.
HOGENOMHOG000256816.
HOVERGENHBG056828.
InParanoidQ8K057.
OMAADELYSC.
OrthoDBEOG7T4MJK.
PhylomeDBQ8K057.
TreeFamTF106117.

Gene expression databases

ArrayExpressQ8K057.
BgeeQ8K057.
GenevestigatorQ8K057.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 3 hits.
[Graphical view]
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
SSF50998. SSF50998. 1 hit.
PROSITEPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio326842.
PROQ8K057.
SOURCESearch...

Entry information

Entry nameIFT80_MOUSE
AccessionPrimary (citable) accession number: Q8K057
Secondary accession number(s): Q6ZPT0, Q8C9F1, Q91YV4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot