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Q8K045 (PKN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase N3

EC=2.7.11.13
Alternative name(s):
Protein kinase PKN-beta
Protein-kinase C-related kinase 3
Gene names
Name:Pkn3
Synonyms:Pknbeta
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to invasiveness in malignant prostate cancer. Ref.2

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation By similarity.

Subcellular location

Nucleus By similarity. Cytoplasmperinuclear region By similarity. Note: Nuclear and perinuclear Golgi region By similarity.

Domain

The C1 domain does not bind the diacylglycerol (DAG).

Post-translational modification

Autophosphorylated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 3 REM (Hr1) repeats.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 878878Serine/threonine-protein kinase N3
PRO_0000055726

Regions

Repeat15 – 8773REM 1
Repeat98 – 16972REM 2
Repeat171 – 24979REM 3
Domain548 – 807260Protein kinase
Domain808 – 87871AGC-kinase C-terminal
Nucleotide binding554 – 5629ATP By similarity
Compositional bias456 – 54388Pro-rich

Sites

Active site6731Proton acceptor By similarity
Binding site5771ATP By similarity

Amino acid modifications

Modified residue7071Phosphothreonine By similarity
Modified residue7111Phosphothreonine By similarity
Modified residue8491Phosphothreonine Probable

Sequences

Sequence LengthMass (Da)Tools
Q8K045 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: F4DC174A6E89047D

FASTA87897,881
        10         20         30         40         50         60 
MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV QQLLRASNRR 

        70         80         90        100        110        120 
LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS EALHRQLQVE LKVKQGAENM 

       130        140        150        160        170        180 
IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA LLRMKISSLE SSGSPEPGPD LLAEELQHRL 

       190        200        210        220        230        240 
RVEAAVAAGA KNVVKLLGGQ RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH 

       250        260        270        280        290        300 
SLRSRVTQEL WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS 

       310        320        330        340        350        360 
PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS FIISLDRARE 

       370        380        390        400        410        420 
LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG RLFAQVTFCE PVIERRPRLQ 

       430        440        450        460        470        480 
RQRCIFSKRR GRDFMRASQM NLSMAAWGRL VMSLLPPCSS PNTASPPKGR PSTAVCGTPS 

       490        500        510        520        530        540 
AASPSNFLPM KTLSKEDTKP PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR 

       550        560        570        580        590        600 
KPPRLQDFRC LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL 

       610        620        630        640        650        660 
ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA CFYLACVVLG 

       670        680        690        700        710        720 
LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG FGDRTSTFCG TPEFLAPEVL 

       730        740        750        760        770        780 
TQEAYTRAVD WWGLGVLLYE MLVGECPFPG DTEEEVFECI VSADVPCPHF LSVQGLELIQ 

       790        800        810        820        830        840 
KLLQKSPEKR LGAGERDAEE IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE 

       850        860        870 
FTSLPPTLTP PVSQSSLTAR QQAAFRDFDF VSEQFLES 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[2]"PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase."
Leenders F., Moepert K., Schmiedeknecht A., Santel A., Czauderna F., Aleku M., Penschuck S., Dames S., Sternberger M., Roehl T., Wellmann A., Arnold W., Giese K., Kaufmann J., Klippel A.
EMBO J. 23:3303-3313(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MALIGNANT CELL GROWTH.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC034126 mRNA. Translation: AAH34126.1.
CCDSCCDS15867.1.
RefSeqNP_722500.1. NM_153805.1.
UniGeneMm.233843.

3D structure databases

ProteinModelPortalQ8K045.
SMRQ8K045. Positions 24-75, 102-156, 517-873.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000041025.

PTM databases

PhosphoSiteQ8K045.

Proteomic databases

PaxDbQ8K045.
PRIDEQ8K045.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785.
GeneID263803.
KEGGmmu:263803.
UCSCuc008jbb.1. mouse.

Organism-specific databases

CTD29941.
MGIMGI:2388285. Pkn3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117322.
HOGENOMHOG000233032.
HOVERGENHBG108317.
InParanoidQ8K045.
KOK06071.
OMAQPFFRTT.
OrthoDBEOG7X9G6Q.
PhylomeDBQ8K045.
TreeFamTF102005.

Gene expression databases

ArrayExpressQ8K045.
BgeeQ8K045.
CleanExMM_PKN3.
GenevestigatorQ8K045.

Family and domain databases

Gene3D1.10.287.160. 3 hits.
2.60.40.150. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTSM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio392137.
PROQ8K045.
SOURCESearch...

Entry information

Entry namePKN3_MOUSE
AccessionPrimary (citable) accession number: Q8K045
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot