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Protein

Serine/threonine-protein kinase N3

Gene

Pkn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to invasiveness in malignant prostate cancer.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771ATPPROSITE-ProRule annotation
Active sitei673 – 6731Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi554 – 5629ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase C activity Source: UniProtKB-EC

GO - Biological processi

  1. epithelial cell migration Source: MGI
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase N3 (EC:2.7.11.13)
Alternative name(s):
Protein kinase PKN-beta
Protein-kinase C-related kinase 3
Gene namesi
Name:Pkn3
Synonyms:Pknbeta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2388285. Pkn3.

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Nuclear and perinuclear Golgi region.By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Serine/threonine-protein kinase N3PRO_0000055726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei707 – 7071PhosphothreonineBy similarity
Modified residuei711 – 7111PhosphothreonineBy similarity
Modified residuei849 – 8491PhosphothreonineCurated

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K045.
PaxDbiQ8K045.
PRIDEiQ8K045.

PTM databases

PhosphoSiteiQ8K045.

Expressioni

Gene expression databases

BgeeiQ8K045.
CleanExiMM_PKN3.
ExpressionAtlasiQ8K045. baseline and differential.
GenevestigatoriQ8K045.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041025.

Structurei

3D structure databases

ProteinModelPortaliQ8K045.
SMRiQ8K045. Positions 24-75, 102-156, 540-872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati15 – 8773REM 1Add
BLAST
Repeati98 – 16972REM 2Add
BLAST
Repeati171 – 24979REM 3Add
BLAST
Domaini548 – 807260Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini808 – 87871AGC-kinase C-terminalAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi456 – 54388Pro-richAdd
BLAST

Domaini

The C1 domain does not bind the diacylglycerol (DAG).

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 3 REM (Hr1) repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120523.
HOGENOMiHOG000233032.
HOVERGENiHBG108317.
InParanoidiQ8K045.
KOiK06071.
OMAiQPFFRTT.
OrthoDBiEOG7X9G6Q.
PhylomeDBiQ8K045.
TreeFamiTF102005.

Family and domain databases

Gene3Di1.10.287.160. 3 hits.
2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV
60 70 80 90 100
QQLLRASNRR LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS
110 120 130 140 150
EALHRQLQVE LKVKQGAENM IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA
160 170 180 190 200
LLRMKISSLE SSGSPEPGPD LLAEELQHRL RVEAAVAAGA KNVVKLLGGQ
210 220 230 240 250
RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH SLRSRVTQEL
260 270 280 290 300
WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS
310 320 330 340 350
PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS
360 370 380 390 400
FIISLDRARE LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG
410 420 430 440 450
RLFAQVTFCE PVIERRPRLQ RQRCIFSKRR GRDFMRASQM NLSMAAWGRL
460 470 480 490 500
VMSLLPPCSS PNTASPPKGR PSTAVCGTPS AASPSNFLPM KTLSKEDTKP
510 520 530 540 550
PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR KPPRLQDFRC
560 570 580 590 600
LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL
610 620 630 640 650
ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA
660 670 680 690 700
CFYLACVVLG LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG
710 720 730 740 750
FGDRTSTFCG TPEFLAPEVL TQEAYTRAVD WWGLGVLLYE MLVGECPFPG
760 770 780 790 800
DTEEEVFECI VSADVPCPHF LSVQGLELIQ KLLQKSPEKR LGAGERDAEE
810 820 830 840 850
IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE FTSLPPTLTP
860 870
PVSQSSLTAR QQAAFRDFDF VSEQFLES
Length:878
Mass (Da):97,881
Last modified:October 1, 2002 - v1
Checksum:iF4DC174A6E89047D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC034126 mRNA. Translation: AAH34126.1.
CCDSiCCDS15867.1.
RefSeqiNP_722500.1. NM_153805.1.
UniGeneiMm.233843.

Genome annotation databases

EnsembliENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785.
GeneIDi263803.
KEGGimmu:263803.
UCSCiuc008jbb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC034126 mRNA. Translation: AAH34126.1.
CCDSiCCDS15867.1.
RefSeqiNP_722500.1. NM_153805.1.
UniGeneiMm.233843.

3D structure databases

ProteinModelPortaliQ8K045.
SMRiQ8K045. Positions 24-75, 102-156, 540-872.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041025.

PTM databases

PhosphoSiteiQ8K045.

Proteomic databases

MaxQBiQ8K045.
PaxDbiQ8K045.
PRIDEiQ8K045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785.
GeneIDi263803.
KEGGimmu:263803.
UCSCiuc008jbb.1. mouse.

Organism-specific databases

CTDi29941.
MGIiMGI:2388285. Pkn3.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120523.
HOGENOMiHOG000233032.
HOVERGENiHBG108317.
InParanoidiQ8K045.
KOiK06071.
OMAiQPFFRTT.
OrthoDBiEOG7X9G6Q.
PhylomeDBiQ8K045.
TreeFamiTF102005.

Miscellaneous databases

NextBioi392137.
PROiQ8K045.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K045.
CleanExiMM_PKN3.
ExpressionAtlasiQ8K045. baseline and differential.
GenevestigatoriQ8K045.

Family and domain databases

Gene3Di1.10.287.160. 3 hits.
2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  2. Cited for: FUNCTION IN MALIGNANT CELL GROWTH.

Entry informationi

Entry nameiPKN3_MOUSE
AccessioniPrimary (citable) accession number: Q8K045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2002
Last modified: March 4, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.