Serine/threonine-protein kinase N3 - Mus musculus (Mouse)

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Q8K045 (PKN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase N3
EC=2.7.11.13

Alternative name(s):
Protein kinase PKN-beta
Protein-kinase C-related kinase 3

Gene names

Name:	Pkn3
Synonyms:	Pknbeta

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to invasiveness in malignant prostate cancer. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm › perinuclear region By similarity. Note: Nuclear and perinuclear Golgi region By similarity.
Domain	The C1 domain does not bind the diacylglycerol (DAG).
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	epithelial cell migration Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 878

878

Serine/threonine-protein kinase N3

PRO_0000055726

Regions

Repeat

15 – 87

REM 1

Repeat

98 – 169

REM 2

Repeat

171 – 249

REM 3

Domain

548 – 807

260

Protein kinase

Domain

808 – 878

AGC-kinase C-terminal

Nucleotide binding

554 – 562

ATP By similarity

Compositional bias

456 – 543

Pro-rich

Sites

Active site

673

Proton acceptor By similarity

Binding site

577

ATP By similarity

Amino acid modifications

Modified residue

707

Phosphothreonine By similarity

Modified residue

711

Phosphothreonine By similarity

Modified residue

849

Phosphothreonine Probable

Sequences

Sequence

Length

Mass (Da)

Tools

Q8K045 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: F4DC174A6E89047D
Show »

FASTA

878

97,881

        10         20         30         40         50         60 
MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV QQLLRASNRR 

        70         80         90        100        110        120 
LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS EALHRQLQVE LKVKQGAENM 

       130        140        150        160        170        180 
IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA LLRMKISSLE SSGSPEPGPD LLAEELQHRL 

       190        200        210        220        230        240 
RVEAAVAAGA KNVVKLLGGQ RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH 

       250        260        270        280        290        300 
SLRSRVTQEL WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS 

       310        320        330        340        350        360 
PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS FIISLDRARE 

       370        380        390        400        410        420 
LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG RLFAQVTFCE PVIERRPRLQ 

       430        440        450        460        470        480 
RQRCIFSKRR GRDFMRASQM NLSMAAWGRL VMSLLPPCSS PNTASPPKGR PSTAVCGTPS 

       490        500        510        520        530        540 
AASPSNFLPM KTLSKEDTKP PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR 

       550        560        570        580        590        600 
KPPRLQDFRC LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL 

       610        620        630        640        650        660 
ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA CFYLACVVLG 

       670        680        690        700        710        720 
LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG FGDRTSTFCG TPEFLAPEVL 

       730        740        750        760        770        780 
TQEAYTRAVD WWGLGVLLYE MLVGECPFPG DTEEEVFECI VSADVPCPHF LSVQGLELIQ 

       790        800        810        820        830        840 
KLLQKSPEKR LGAGERDAEE IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE 

       850        860        870 
FTSLPPTLTP PVSQSSLTAR QQAAFRDFDF VSEQFLES

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[2]	"PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase." Leenders F., Moepert K., Schmiedeknecht A., Santel A., Czauderna F., Aleku M., Penschuck S., Dames S., Sternberger M., Roehl T., Wellmann A., Arnold W., Giese K., Kaufmann J., Klippel A. EMBO J. 23:3303-3313(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN MALIGNANT CELL GROWTH.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC034126 mRNA. Translation: AAH34126.1.
IPI	IPI00169493.
RefSeq	NP_722500.1. NM_153805.1.
UniGene	Mm.233843.
3D structure databases
ProteinModelPortal	Q8K045.
SMR	Q8K045. Positions 24-75, 102-165, 517-877.
ModBase	Search...
PTM databases
PhosphoSite	Q8K045.
Proteomic databases
PaxDb	Q8K045.
PRIDE	Q8K045.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785.
GeneID	263803.
KEGG	mmu:263803.
Organism-specific databases
CTD	29941.
MGI	MGI:2388285. Pkn3.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104096.
HOGENOM	HOG000233032.
HOVERGEN	HBG108317.
InParanoid	Q8K045.
KO	K06071.
OMA	IFSKRRG.
OrthoDB	EOG40ZQWW.
Gene expression databases
ArrayExpress	Q8K045.
Bgee	Q8K045.
CleanEx	MM_PKN3.
Genevestigator	Q8K045.
GermOnline	ENSMUSG00000026785. Mus musculus.
Family and domain databases
Gene3D	1.10.287.160. 3 hits.
InterPro	IPR000961. AGC-kinase_C. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011072. HR1_rho-bd. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit. SSF46585. PKN_effector. 3 hits.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	392137.
SOURCE	Search...

Entry information

Entry name

PKN3_MOUSE

Accession

Primary (citable) accession number: Q8K045

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents