Reviewed, UniProtKB/Swiss-Prot Q8K045 (PKN3_MOUSE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase N3
    EC=2.7.11.13
Alternative name(s):
    Protein kinase PKN-beta
    Protein-kinase C-related kinase 3

Gene names

Name:	Pkn3
Synonyms:	Pknbeta

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Contributes to invasiveness in malignant prostate cancer. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm › perinuclear region By similarity. Note: Nuclear and perinuclear Golgi region By similarity.
Domain	The C1 domain does not bind the diacylglycerol (DAG).
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Serine/threonine-protein kinase N3		PRO_0000055726
Regions
	Repeat	15 – 87	73	REM 1
	Repeat	98 – 169	72	REM 2
	Repeat	171 – 249	79	REM 3
	Domain	548 – 807	260	Protein kinase
	Domain	808 – 878	71	AGC-kinase C-terminal
	Nucleotide binding	554 – 562	9	ATP By similarity
	Compositional bias	456 – 543	88	Pro-rich
Sites
	Active site	673	1	Proton acceptor By similarity
	Binding site	577	1	ATP By similarity
Amino acid modifications
	Modified residue	300	1	Phosphoserine By similarity
	Modified residue	539	1	Phosphothreonine By similarity
	Modified residue	707	1	Phosphothreonine By similarity
	Modified residue	711	1	Phosphothreonine By similarity
	Modified residue	849	1	Phosphothreonine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q8K045-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: F4DC174A6E89047D	FASTA	878	97,881
	10 20 30 40 50 60 MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV QQLLRASNRR 70 80 90 100 110 120 LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS EALHRQLQVE LKVKQGAENM 130 140 150 160 170 180 IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA LLRMKISSLE SSGSPEPGPD LLAEELQHRL 190 200 210 220 230 240 RVEAAVAAGA KNVVKLLGGQ RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH 250 260 270 280 290 300 SLRSRVTQEL WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS 310 320 330 340 350 360 PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS FIISLDRARE 370 380 390 400 410 420 LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG RLFAQVTFCE PVIERRPRLQ 430 440 450 460 470 480 RQRCIFSKRR GRDFMRASQM NLSMAAWGRL VMSLLPPCSS PNTASPPKGR PSTAVCGTPS 490 500 510 520 530 540 AASPSNFLPM KTLSKEDTKP PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR 550 560 570 580 590 600 KPPRLQDFRC LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL 610 620 630 640 650 660 ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA CFYLACVVLG 670 680 690 700 710 720 LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG FGDRTSTFCG TPEFLAPEVL 730 740 750 760 770 780 TQEAYTRAVD WWGLGVLLYE MLVGECPFPG DTEEEVFECI VSADVPCPHF LSVQGLELIQ 790 800 810 820 830 840 KLLQKSPEKR LGAGERDAEE IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE 850 860 870 FTSLPPTLTP PVSQSSLTAR QQAAFRDFDF VSEQFLES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[2]	"PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase." Leenders F., Moepert K., Schmiedeknecht A., Santel A., Czauderna F., Aleku M., Penschuck S., Dames S., Sternberger M., Roehl T., Wellmann A., Arnold W., Giese K., Kaufmann J., Klippel A. EMBO J. 23:3303-3313(2004) [PubMed: 15282551] [Abstract] Cited for: FUNCTION IN MALIGNANT CELL GROWTH.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC034126 mRNA. Translation: AAH34126.1.
IPI	IPI00169493.
RefSeq	NP_722500.1.
UniGene	Mm.233843
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
PTM databases
PhosphoSite	Q8K045.
Proteomic databases
PRIDE	Q8K045.
Genome annotation databases
Ensembl	ENSMUSG00000026785. Mus musculus. [Contig view]
GeneID	263803.
KEGG	mmu:263803.
Organism-specific databases
MGI	MGI:2388285. Pkn3.
Phylogenomic databases
HOGENOM	Q8K045.
HOVERGEN	Q8K045.
OMA	Q8K045. IFSKRRG.
Enzyme and pathway databases
BRENDA	2.7.11.13. 244.
Gene expression databases
Bgee	Q8K045.
CleanEx	MM_PKN3.
GermOnline	ENSMUSG00000026785. Mus musculus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR017892. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:1.10.287.160. HR1_rho-bd. 3 hits.
Pfam	PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	392137.
SOURCE	Search...

Entry information

Entry name

PKN3_MOUSE

Accession

Primary (citable) accession number: Q8K045

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)