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Q8K045

- PKN3_MOUSE

UniProt

Q8K045 - PKN3_MOUSE

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Protein

Serine/threonine-protein kinase N3

Gene
Pkn3, Pknbeta
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Contributes to invasiveness in malignant prostate cancer.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771ATP By similarity
Active sitei673 – 6731Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi554 – 5629ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase C activity Source: UniProtKB-EC

GO - Biological processi

  1. epithelial cell migration Source: Ensembl
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase N3 (EC:2.7.11.13)
Alternative name(s):
Protein kinase PKN-beta
Protein-kinase C-related kinase 3
Gene namesi
Name:Pkn3
Synonyms:Pknbeta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2388285. Pkn3.

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Nuclear and perinuclear Golgi region By similarity.

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Serine/threonine-protein kinase N3PRO_0000055726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei707 – 7071Phosphothreonine By similarity
Modified residuei711 – 7111Phosphothreonine By similarity
Modified residuei849 – 8491Phosphothreonine Inferred

Post-translational modificationi

Autophosphorylated By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K045.
PRIDEiQ8K045.

PTM databases

PhosphoSiteiQ8K045.

Expressioni

Gene expression databases

ArrayExpressiQ8K045.
BgeeiQ8K045.
CleanExiMM_PKN3.
GenevestigatoriQ8K045.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041025.

Structurei

3D structure databases

ProteinModelPortaliQ8K045.
SMRiQ8K045. Positions 24-75, 102-156, 517-873.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati15 – 8773REM 1Add
BLAST
Repeati98 – 16972REM 2Add
BLAST
Repeati171 – 24979REM 3Add
BLAST
Domaini548 – 807260Protein kinaseAdd
BLAST
Domaini808 – 87871AGC-kinase C-terminalAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi456 – 54388Pro-richAdd
BLAST

Domaini

The C1 domain does not bind the diacylglycerol (DAG).

Sequence similaritiesi

Contains 3 REM (Hr1) repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117322.
HOGENOMiHOG000233032.
HOVERGENiHBG108317.
InParanoidiQ8K045.
KOiK06071.
OMAiQPFFRTT.
OrthoDBiEOG7X9G6Q.
PhylomeDBiQ8K045.
TreeFamiTF102005.

Family and domain databases

Gene3Di1.10.287.160. 3 hits.
2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K045-1 [UniParc]FASTAAdd to Basket

« Hide

MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV    50
QQLLRASNRR LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS 100
EALHRQLQVE LKVKQGAENM IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA 150
LLRMKISSLE SSGSPEPGPD LLAEELQHRL RVEAAVAAGA KNVVKLLGGQ 200
RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH SLRSRVTQEL 250
WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS 300
PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS 350
FIISLDRARE LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG 400
RLFAQVTFCE PVIERRPRLQ RQRCIFSKRR GRDFMRASQM NLSMAAWGRL 450
VMSLLPPCSS PNTASPPKGR PSTAVCGTPS AASPSNFLPM KTLSKEDTKP 500
PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR KPPRLQDFRC 550
LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL 600
ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA 650
CFYLACVVLG LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG 700
FGDRTSTFCG TPEFLAPEVL TQEAYTRAVD WWGLGVLLYE MLVGECPFPG 750
DTEEEVFECI VSADVPCPHF LSVQGLELIQ KLLQKSPEKR LGAGERDAEE 800
IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE FTSLPPTLTP 850
PVSQSSLTAR QQAAFRDFDF VSEQFLES 878
Length:878
Mass (Da):97,881
Last modified:October 1, 2002 - v1
Checksum:iF4DC174A6E89047D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC034126 mRNA. Translation: AAH34126.1.
CCDSiCCDS15867.1.
RefSeqiNP_722500.1. NM_153805.1.
UniGeneiMm.233843.

Genome annotation databases

EnsembliENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785.
GeneIDi263803.
KEGGimmu:263803.
UCSCiuc008jbb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC034126 mRNA. Translation: AAH34126.1 .
CCDSi CCDS15867.1.
RefSeqi NP_722500.1. NM_153805.1.
UniGenei Mm.233843.

3D structure databases

ProteinModelPortali Q8K045.
SMRi Q8K045. Positions 24-75, 102-156, 517-873.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000041025.

PTM databases

PhosphoSitei Q8K045.

Proteomic databases

PaxDbi Q8K045.
PRIDEi Q8K045.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045246 ; ENSMUSP00000041025 ; ENSMUSG00000026785 .
GeneIDi 263803.
KEGGi mmu:263803.
UCSCi uc008jbb.1. mouse.

Organism-specific databases

CTDi 29941.
MGIi MGI:2388285. Pkn3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117322.
HOGENOMi HOG000233032.
HOVERGENi HBG108317.
InParanoidi Q8K045.
KOi K06071.
OMAi QPFFRTT.
OrthoDBi EOG7X9G6Q.
PhylomeDBi Q8K045.
TreeFami TF102005.

Miscellaneous databases

NextBioi 392137.
PROi Q8K045.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8K045.
Bgeei Q8K045.
CleanExi MM_PKN3.
Genevestigatori Q8K045.

Family and domain databases

Gene3Di 1.10.287.160. 3 hits.
2.60.40.150. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view ]
SMARTi SM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  2. Cited for: FUNCTION IN MALIGNANT CELL GROWTH.

Entry informationi

Entry nameiPKN3_MOUSE
AccessioniPrimary (citable) accession number: Q8K045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi