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Q8K045

- PKN3_MOUSE

UniProt

Q8K045 - PKN3_MOUSE

Protein

Serine/threonine-protein kinase N3

Gene

Pkn3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Contributes to invasiveness in malignant prostate cancer.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei577 – 5771ATPPROSITE-ProRule annotation
    Active sitei673 – 6731Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi554 – 5629ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase C activity Source: UniProtKB-EC

    GO - Biological processi

    1. epithelial cell migration Source: Ensembl
    2. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase N3 (EC:2.7.11.13)
    Alternative name(s):
    Protein kinase PKN-beta
    Protein-kinase C-related kinase 3
    Gene namesi
    Name:Pkn3
    Synonyms:Pknbeta
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2388285. Pkn3.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmperinuclear region By similarity
    Note: Nuclear and perinuclear Golgi region.By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 878878Serine/threonine-protein kinase N3PRO_0000055726Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei707 – 7071PhosphothreonineBy similarity
    Modified residuei711 – 7111PhosphothreonineBy similarity
    Modified residuei849 – 8491PhosphothreonineCurated

    Post-translational modificationi

    Autophosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8K045.
    PRIDEiQ8K045.

    PTM databases

    PhosphoSiteiQ8K045.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K045.
    BgeeiQ8K045.
    CleanExiMM_PKN3.
    GenevestigatoriQ8K045.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000041025.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K045.
    SMRiQ8K045. Positions 24-75, 102-156, 517-873.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati15 – 8773REM 1Add
    BLAST
    Repeati98 – 16972REM 2Add
    BLAST
    Repeati171 – 24979REM 3Add
    BLAST
    Domaini548 – 807260Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini808 – 87871AGC-kinase C-terminalAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi456 – 54388Pro-richAdd
    BLAST

    Domaini

    The C1 domain does not bind the diacylglycerol (DAG).

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 3 REM (Hr1) repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117322.
    HOGENOMiHOG000233032.
    HOVERGENiHBG108317.
    InParanoidiQ8K045.
    KOiK06071.
    OMAiQPFFRTT.
    OrthoDBiEOG7X9G6Q.
    PhylomeDBiQ8K045.
    TreeFamiTF102005.

    Family and domain databases

    Gene3Di1.10.287.160. 3 hits.
    2.60.40.150. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR000008. C2_dom.
    IPR011072. HR1_rho-bd.
    IPR011009. Kinase-like_dom.
    IPR017892. Pkinase_C.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF02185. HR1. 3 hits.
    PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view]
    SMARTiSM00742. Hr1. 3 hits.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF46585. SSF46585. 3 hits.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K045-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV    50
    QQLLRASNRR LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS 100
    EALHRQLQVE LKVKQGAENM IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA 150
    LLRMKISSLE SSGSPEPGPD LLAEELQHRL RVEAAVAAGA KNVVKLLGGQ 200
    RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH SLRSRVTQEL 250
    WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS 300
    PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS 350
    FIISLDRARE LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG 400
    RLFAQVTFCE PVIERRPRLQ RQRCIFSKRR GRDFMRASQM NLSMAAWGRL 450
    VMSLLPPCSS PNTASPPKGR PSTAVCGTPS AASPSNFLPM KTLSKEDTKP 500
    PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR KPPRLQDFRC 550
    LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL 600
    ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA 650
    CFYLACVVLG LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG 700
    FGDRTSTFCG TPEFLAPEVL TQEAYTRAVD WWGLGVLLYE MLVGECPFPG 750
    DTEEEVFECI VSADVPCPHF LSVQGLELIQ KLLQKSPEKR LGAGERDAEE 800
    IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE FTSLPPTLTP 850
    PVSQSSLTAR QQAAFRDFDF VSEQFLES 878
    Length:878
    Mass (Da):97,881
    Last modified:October 1, 2002 - v1
    Checksum:iF4DC174A6E89047D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC034126 mRNA. Translation: AAH34126.1.
    CCDSiCCDS15867.1.
    RefSeqiNP_722500.1. NM_153805.1.
    UniGeneiMm.233843.

    Genome annotation databases

    EnsembliENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785.
    GeneIDi263803.
    KEGGimmu:263803.
    UCSCiuc008jbb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC034126 mRNA. Translation: AAH34126.1 .
    CCDSi CCDS15867.1.
    RefSeqi NP_722500.1. NM_153805.1.
    UniGenei Mm.233843.

    3D structure databases

    ProteinModelPortali Q8K045.
    SMRi Q8K045. Positions 24-75, 102-156, 517-873.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000041025.

    PTM databases

    PhosphoSitei Q8K045.

    Proteomic databases

    PaxDbi Q8K045.
    PRIDEi Q8K045.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045246 ; ENSMUSP00000041025 ; ENSMUSG00000026785 .
    GeneIDi 263803.
    KEGGi mmu:263803.
    UCSCi uc008jbb.1. mouse.

    Organism-specific databases

    CTDi 29941.
    MGIi MGI:2388285. Pkn3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117322.
    HOGENOMi HOG000233032.
    HOVERGENi HBG108317.
    InParanoidi Q8K045.
    KOi K06071.
    OMAi QPFFRTT.
    OrthoDBi EOG7X9G6Q.
    PhylomeDBi Q8K045.
    TreeFami TF102005.

    Miscellaneous databases

    NextBioi 392137.
    PROi Q8K045.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K045.
    Bgeei Q8K045.
    CleanExi MM_PKN3.
    Genevestigatori Q8K045.

    Family and domain databases

    Gene3Di 1.10.287.160. 3 hits.
    2.60.40.150. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR000008. C2_dom.
    IPR011072. HR1_rho-bd.
    IPR011009. Kinase-like_dom.
    IPR017892. Pkinase_C.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF02185. HR1. 3 hits.
    PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00742. Hr1. 3 hits.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46585. SSF46585. 3 hits.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    2. Cited for: FUNCTION IN MALIGNANT CELL GROWTH.

    Entry informationi

    Entry nameiPKN3_MOUSE
    AccessioniPrimary (citable) accession number: Q8K045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3