Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase N3

Gene

Pkn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to invasiveness in malignant prostate cancer.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei577ATPPROSITE-ProRule annotation1
Active sitei673Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi554 – 562ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5625740 RHO GTPases activate PKNs

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase N3 (EC:2.7.11.13)
Alternative name(s):
Protein kinase PKN-beta
Protein-kinase C-related kinase 3
Gene namesi
Name:Pkn3
Synonyms:Pknbeta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2388285 Pkn3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557261 – 878Serine/threonine-protein kinase N3Add BLAST878

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164PhosphoserineBy similarity1
Modified residuei707PhosphothreonineBy similarity1
Modified residuei711PhosphothreonineBy similarity1
Modified residuei849PhosphothreonineBy similarityCurated1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K045
PaxDbiQ8K045
PRIDEiQ8K045

PTM databases

iPTMnetiQ8K045
PhosphoSitePlusiQ8K045

Expressioni

Gene expression databases

BgeeiENSMUSG00000026785
CleanExiMM_PKN3
ExpressionAtlasiQ8K045 baseline and differential
GenevisibleiQ8K045 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041025

Structurei

3D structure databases

ProteinModelPortaliQ8K045
SMRiQ8K045
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 77REM-1 1PROSITE-ProRule annotationAdd BLAST76
Domaini86 – 165REM-1 2PROSITE-ProRule annotationAdd BLAST80
Domaini169 – 238REM-1 3PROSITE-ProRule annotationAdd BLAST70
Domaini548 – 807Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini808 – 878AGC-kinase C-terminalAdd BLAST71

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi456 – 543Pro-richAdd BLAST88

Domaini

The C1 domain does not bind the diacylglycerol (DAG).

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0694 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00820000126964
HOGENOMiHOG000233032
HOVERGENiHBG108317
InParanoidiQ8K045
KOiK06071
OMAiCPEKRLG
OrthoDBiEOG091G00YT
PhylomeDBiQ8K045
TreeFamiTF102005

Family and domain databases

CDDicd11622 HR1_PKN_1, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011072 HR1_rho-bd
IPR036274 HR1_rpt_sf
IPR011009 Kinase-like_dom_sf
IPR017892 Pkinase_C
IPR037313 PKN_HR1_1
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF02185 HR1, 3 hits
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
SMARTiView protein in SMART
SM00742 Hr1, 3 hits
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF46585 SSF46585, 3 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS51860 REM_1, 3 hits

Sequencei

Sequence statusi: Complete.

Q8K045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV
60 70 80 90 100
QQLLRASNRR LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS
110 120 130 140 150
EALHRQLQVE LKVKQGAENM IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA
160 170 180 190 200
LLRMKISSLE SSGSPEPGPD LLAEELQHRL RVEAAVAAGA KNVVKLLGGQ
210 220 230 240 250
RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH SLRSRVTQEL
260 270 280 290 300
WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS
310 320 330 340 350
PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS
360 370 380 390 400
FIISLDRARE LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG
410 420 430 440 450
RLFAQVTFCE PVIERRPRLQ RQRCIFSKRR GRDFMRASQM NLSMAAWGRL
460 470 480 490 500
VMSLLPPCSS PNTASPPKGR PSTAVCGTPS AASPSNFLPM KTLSKEDTKP
510 520 530 540 550
PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR KPPRLQDFRC
560 570 580 590 600
LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL
610 620 630 640 650
ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA
660 670 680 690 700
CFYLACVVLG LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG
710 720 730 740 750
FGDRTSTFCG TPEFLAPEVL TQEAYTRAVD WWGLGVLLYE MLVGECPFPG
760 770 780 790 800
DTEEEVFECI VSADVPCPHF LSVQGLELIQ KLLQKSPEKR LGAGERDAEE
810 820 830 840 850
IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE FTSLPPTLTP
860 870
PVSQSSLTAR QQAAFRDFDF VSEQFLES
Length:878
Mass (Da):97,881
Last modified:October 1, 2002 - v1
Checksum:iF4DC174A6E89047D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC034126 mRNA Translation: AAH34126.1
CCDSiCCDS15867.1
RefSeqiNP_722500.1, NM_153805.1
UniGeneiMm.233843

Genome annotation databases

EnsembliENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785
GeneIDi263803
KEGGimmu:263803
UCSCiuc008jbb.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPKN3_MOUSE
AccessioniPrimary (citable) accession number: Q8K045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health