Reviewed,
UniProtKB/Swiss-Prot Q8K045 (PKN3_MOUSE)
Last modified
June 16, 2009.
Version 62.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase N3 EC=2.7.11.13 Alternative name(s): Protein kinase PKN-beta Protein-kinase C-related kinase 3 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 878 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Contributes to invasiveness in malignant prostate cancer. Ref.2 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation By similarity. |
| Subcellular location | Nucleus By similarity. Cytoplasm › perinuclear region By similarity. Note: Nuclear and perinuclear Golgi region By similarity. |
| Domain | The C1 domain does not bind the diacylglycerol (DAG). |
| Post-translational modification | Autophosphorylated By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Nucleus |
| Domain | Repeat |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transductionInferred from electronic annotation. Source: InterPro |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasmInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 878 | 878 | Serine/threonine-protein kinase N3 | PRO_0000055726 | |||||
Regions | |||||||||
| Repeat | 15 – 87 | 73 | REM 1 | ||||||
| Repeat | 98 – 169 | 72 | REM 2 | ||||||
| Repeat | 171 – 249 | 79 | REM 3 | ||||||
| Domain | 548 – 807 | 260 | Protein kinase | ||||||
| Domain | 808 – 878 | 71 | AGC-kinase C-terminal | ||||||
| Nucleotide binding | 554 – 562 | 9 | ATP By similarity | ||||||
| Compositional bias | 456 – 543 | 88 | Pro-rich | ||||||
Sites | |||||||||
| Active site | 673 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 577 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 300 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 539 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 707 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 711 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 849 | 1 | Phosphothreonine Probable | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland. |
| [2] | "PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase." Leenders F., Moepert K., Schmiedeknecht A., Santel A., Czauderna F., Aleku M., Penschuck S., Dames S., Sternberger M., Roehl T., Wellmann A., Arnold W., Giese K., Kaufmann J., Klippel A. EMBO J. 23:3303-3313(2004) [PubMed: 15282551] [Abstract] Cited for: FUNCTION IN MALIGNANT CELL GROWTH. |
Cross-references
Sequence databases | |
|---|---|
| BC034126 mRNA. Translation: AAH34126.1. | |
| IPI | IPI00169493. |
| RefSeq | NP_722500.1. |
| UniGene | Mm.233843 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GZK based on UniProtKB P31751. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q8K045. |
Proteomic databases | |
| PRIDE | Q8K045. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000026785. Mus musculus. [Contig view] |
| GeneID | 263803. |
| KEGG | mmu:263803. |
Organism-specific databases | |
| MGI | MGI:2388285. Pkn3. |
Phylogenomic databases | |
| HOGENOM | Q8K045. |
| HOVERGEN | Q8K045. |
| OMA | Q8K045. IFSKRRG. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.13. 244. |
Gene expression databases | |
| Bgee | Q8K045. |
| CleanEx | MM_PKN3. |
| GermOnline | ENSMUSG00000026785. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000961. AGC-kinase_C. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR017892. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Gene3D | G3DSA:1.10.287.160. HR1_rho-bd. 3 hits. |
| Pfam | PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 392137. |
| SOURCE | Search... |
Entry information
| Entry name | PKN3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8K045 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


