ID   AKC1H_MOUSE             Reviewed;         323 AA.
AC   Q8K023; Q99N44;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Aldo-keto reductase family 1 member C18;
DE            EC=1.1.-.-;
DE   AltName: Full=20-alpha-hydroxysteroid dehydrogenase;
DE            Short=20-alpha-HSD;
DE            EC=1.1.1.149;
GN   Name=Akr1c18;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=ICR; TISSUE=Ovary;
RA   Ishida M., Chang K., Hirabayashi K., Nishihara M., Takahashi M.;
RT   "Cloning of mouse 20alpha-hydroxysteroid dehydrogenase cDNA and its
RT   mRNA localization during pregnancy.";
RL   J. Reprod. Dev. 45:321-329(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=FVB/N; TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the conversion of progesterone into 20-alpha-
CC       dihydroprogesterone (20 alpha-OHP).
CC   -!- CATALYTIC ACTIVITY: 17-alpha,20-alpha-dihydroxypregn-4-en-3-one +
CC       NAD(P)(+) = 17-alpha-hydroxyprogesterone + NAD(P)H.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8K023-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8K023-2; Sequence=VSP_014039;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AB059565; BAB40958.1; -; mRNA.
DR   EMBL; BC034259; AAH34259.1; -; mRNA.
DR   IPI; IPI00118068; -.
DR   IPI; IPI00169481; -.
DR   RefSeq; NP_598827.1; -.
DR   UniGene; Mm.41337; -.
DR   HSSP; P23457; 1LWI.
DR   SMR; Q8K023; 6-323.
DR   PhosphoSite; Q8K023; -.
DR   PRIDE; Q8K023; -.
DR   Ensembl; ENSMUSG00000021214; Mus musculus.
DR   GeneID; 105349; -.
DR   KEGG; mmu:105349; -.
DR   MGI; MGI:2145420; Akr1c18.
DR   HOGENOM; Q8K023; -.
DR   HOVERGEN; Q8K023; -.
DR   OMA; Q8K023; GTQRYKY.
DR   BRENDA; 1.1.1.149; 244.
DR   NextBio; 357616; -.
DR   ArrayExpress; Q8K023; -.
DR   Bgee; Q8K023; -.
DR   CleanEx; MM_AKR1C18; -.
DR   GermOnline; ENSMUSG00000021214; Mus musculus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047006; F:20-alpha-hydroxysteroid dehydrogenase activity; IDA:MGI.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0007567; P:parturition; IMP:MGI.
DR   GO; GO:0006709; P:progesterone catabolic process; IDA:MGI.
DR   InterPro; IPR001395; Aldo/ket_red.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   Gene3D; G3DSA:3.20.20.100; Aldo/ket_red; 1.
DR   PANTHER; PTHR11732; Aldo/ket_red; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   ProDom; PD000288; Aldo/ket_red; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cytoplasm; NADP; Oxidoreductase.
FT   CHAIN         1    323       Aldo-keto reductase family 1 member C18.
FT                                /FTId=PRO_0000124649.
FT   NP_BIND      13     22       NADP (Potential).
FT   NP_BIND     217    280       NADP (By similarity).
FT   ACT_SITE     55     55       Proton donor (By similarity).
FT   BINDING     117    117       Substrate (By similarity).
FT   SITE         54     54       Important for substrate specificity (By
FT                                similarity).
FT   SITE         84     84       Lowers pKa of active site Tyr (By
FT                                similarity).
FT   VAR_SEQ     124    149       Missing (in isoform 2).
FT                                /FTId=VSP_014039.
SQ   SEQUENCE   323 AA;  37177 MW;  F2732C09C60B4544 CRC64;
     MNSKIQKIEL NDGHSIPVLG FGTYATEEHL KKKSMESTKI AIDVGFCHID CSHLYQNEEE
     IGQAILSKIE DGTVKREDIF YTSKLWSTSH RPELVRPSLE NSLRKLNLDY VDLYLIHFPV
     SLKPGNELLP KDEHGNLIFD TVDLCDTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP
     GLKYKPVCNQ VECHLYLNQS KLLAYCKMND IVLVAYGALG TQRYKYCINE DTPVLLDDPV
     LCAMAKKYKR TPALIALRYQ LDRGIVALAK SFNEERIREN MQVFDFQLAS DDMKILDGLD
     RNLRYFPADM FKAHPNFPFF DEY
//