Reviewed, UniProtKB/Swiss-Prot Q8K023 (AKC1H_MOUSE)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C18
    EC=1.1.-.-
Alternative name(s):
    20-alpha-hydroxysteroid dehydrogenase
      Short name=20-alpha-HSD
    EC=1.1.1.149

Gene names

Name:

Akr1c18

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of progesterone into 20-alpha-dihydroprogesterone (20 alpha-OHP).
Catalytic activity	17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW parturition Inferred from mutant phenotype. Source: MGI progesterone catabolic process Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	20-alpha-hydroxysteroid dehydrogenase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K023-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K023-2)

The sequence of this isoform differs from the canonical sequence as follows:
     124-149: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Aldo-keto reductase family 1 member C18		PRO_0000124649
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	54	1	Important for substrate specificity By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity
Natural variations
	Alternative sequence	124 – 149	26	Missing in isoform 2.		VSP_014039

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: F2732C09C60B4544	FASTA	323	37,177
	10 20 30 40 50 60 MNSKIQKIEL NDGHSIPVLG FGTYATEEHL KKKSMESTKI AIDVGFCHID CSHLYQNEEE 70 80 90 100 110 120 IGQAILSKIE DGTVKREDIF YTSKLWSTSH RPELVRPSLE NSLRKLNLDY VDLYLIHFPV 130 140 150 160 170 180 SLKPGNELLP KDEHGNLIFD TVDLCDTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHLYLNQS KLLAYCKMND IVLVAYGALG TQRYKYCINE DTPVLLDDPV 250 260 270 280 290 300 LCAMAKKYKR TPALIALRYQ LDRGIVALAK SFNEERIREN MQVFDFQLAS DDMKILDGLD 310 320 RNLRYFPADM FKAHPNFPFF DEY
	Isoform 2. Checksum: 4028DA1CA5B88D06	FASTA	297	34,224
	10 20 30 40 50 60 MNSKIQKIEL NDGHSIPVLG FGTYATEEHL KKKSMESTKI AIDVGFCHID CSHLYQNEEE 70 80 90 100 110 120 IGQAILSKIE DGTVKREDIF YTSKLWSTSH RPELVRPSLE NSLRKLNLDY VDLYLIHFPV 130 140 150 160 170 180 SLKAMEKCKD AGLAKSIGVS NFNRRQLEMI LNKPGLKYKP VCNQVECHLY LNQSKLLAYC 190 200 210 220 230 240 KMNDIVLVAY GALGTQRYKY CINEDTPVLL DDPVLCAMAK KYKRTPALIA LRYQLDRGIV 250 260 270 280 290 ALAKSFNEER IRENMQVFDF QLASDDMKIL DGLDRNLRYF PADMFKAHPN FPFFDEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of mouse 20alpha-hydroxysteroid dehydrogenase cDNA and its mRNA localization during pregnancy." Ishida M., Chang K., Hirabayashi K., Nishihara M., Takahashi M. J. Reprod. Dev. 45:321-329(1999) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: ICR. Tissue: Ovary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB059565 mRNA. Translation: BAB40958.1. BC034259 mRNA. Translation: AAH34259.1.
IPI	IPI00118068. IPI00169481.
RefSeq	NP_598827.1.
UniGene	Mm.41337
3D structure databases
HSSP	HSSP built from PDB template 1LWI based on UniProtKB P23457.
SMR	Q8K023. Positions 6-323.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K023.
PTM databases
PhosphoSite	Q8K023.
Proteomic databases
PRIDE	Q8K023.
Genome annotation databases
Ensembl	ENSMUST00000021635; ENSMUSP00000021635; ENSMUSG00000021214; Mus musculus. [Genome view] ENSMUST00000110704; ENSMUSP00000106332; ENSMUSG00000021214; Mus musculus. [Genome view]
GeneID	105349.
KEGG	mmu:105349.
UCSC	uc007pjj.1. mouse. uc007pjk.1. mouse.
Organism-specific databases
CTD	105349.
MGI	MGI:2145420. Akr1c18.
Phylogenomic databases
HOGENOM	Q8K023.
HOVERGEN	Q8K023.
OMA	GTQRYKY.
Enzyme and pathway databases
BRENDA	1.1.1.149. 244.
Gene expression databases
ArrayExpress	Q8K023.
Bgee	Q8K023.
CleanEx	MM_AKR1C18.
Genevestigator	Q8K023.
GermOnline	ENSMUSG00000021214. Mus musculus.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_sg. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PRINTS	PR00069. ALDKETRDTASE.
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357616.
SOURCE	Search...

Entry information

Entry name

AKC1H_MOUSE

Accession

Primary (citable) accession number: Q8K023
Secondary accession number(s): Q99N44

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)