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Protein

Bcl-2-associated transcription factor 1

Gene

Bclaf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-associated transcription factor 1
Short name:
Btf
Gene namesi
Name:Bclaf1
Synonyms:Btf, Kiaa0164
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1917580. Bclaf1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Nucleus speckle By similarity
  • Nucleusnucleoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000648891 – 919Bcl-2-associated transcription factor 1Add BLAST919

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphoserineBy similarity1
Modified residuei104PhosphoserineBy similarity1
Modified residuei152N6-acetyllysineBy similarity1
Modified residuei177PhosphoserineCombined sources1
Modified residuei181PhosphoserineBy similarity1
Modified residuei196PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei218PhosphotyrosineBy similarity1
Modified residuei221PhosphoserineCombined sources1
Modified residuei258PhosphoserineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei263PhosphoserineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei283PhosphotyrosineBy similarity1
Modified residuei284PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Modified residuei296PhosphoserineBy similarity1
Modified residuei299PhosphoserineCombined sources1
Modified residuei305PhosphothreonineCombined sources1
Modified residuei314PhosphoserineBy similarity1
Modified residuei330N6-acetyllysine; alternateCombined sources1
Cross-linki330Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei339PhosphothreonineBy similarity1
Modified residuei381PhosphotyrosineBy similarity1
Modified residuei383PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei400PhosphothreonineCombined sources1
Cross-linki411Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei419N6-acetyllysine; alternateCombined sources1
Cross-linki419Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei420PhosphoserineBy similarity1
Modified residuei425PhosphoserineBy similarity1
Modified residuei429PhosphothreonineBy similarity1
Modified residuei435N6-acetyllysine; alternateCombined sources1
Cross-linki435Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei448PhosphoserineBy similarity1
Cross-linki455Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki460Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei470PhosphoserineBy similarity1
Modified residuei473N6-acetyllysineCombined sources1
Cross-linki489Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei494PhosphoserineCombined sources1
Cross-linki499Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei500PhosphoserineBy similarity1
Modified residuei510PhosphoserineCombined sources1
Modified residuei523PhosphoserineBy similarity1
Modified residuei529PhosphoserineCombined sources1
Cross-linki534Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki546Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki548Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei557PhosphoserineBy similarity1
Modified residuei562PhosphoserineBy similarity1
Modified residuei564PhosphothreonineBy similarity1
Modified residuei576PhosphoserineBy similarity1
Cross-linki578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei646PhosphoserineBy similarity1
Modified residuei656PhosphoserineCombined sources1
Modified residuei658PhosphoserineBy similarity1
Cross-linki674Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei688PhosphoserineBy similarity1
Modified residuei759PhosphoserineBy similarity1
Cross-linki777Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei802Citrulline1 Publication1
Modified residuei808Omega-N-methylarginineBy similarity1
Cross-linki830Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki830Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8K019.
PeptideAtlasiQ8K019.
PRIDEiQ8K019.

PTM databases

iPTMnetiQ8K019.
PhosphoSitePlusiQ8K019.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037608.
CleanExiMM_BCLAF1.
ExpressionAtlasiQ8K019. baseline and differential.
GenevisibleiQ8K019. MM.

Interactioni

Subunit structurei

Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN (By similarity). Component of the WTAP complex composed of WTAP, ZC3H13, CBLL1, KIAA1429, RBM15, BCLAF1 and THRAP3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi215443. 6 interactors.
IntActiQ8K019. 4 interactors.
MINTiMINT-4089177.
STRINGi10090.ENSMUSP00000043583.

Structurei

3D structure databases

ProteinModelPortaliQ8K019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi141 – 148Poly-Ser8
Compositional biasi748 – 762Poly-SerAdd BLAST15

Phylogenomic databases

eggNOGiENOG410IJCD. Eukaryota.
ENOG4110X68. LUCA.
GeneTreeiENSGT00530000063211.
HOGENOMiHOG000054209.
HOVERGENiHBG050681.
InParanoidiQ8K019.
KOiK13087.
OMAiDDSKHKS.
OrthoDBiEOG091G03HU.
PhylomeDBiQ8K019.
TreeFamiTF335939.

Family and domain databases

InterProiIPR026668. Bcl-2_assoc_TF1.
IPR029199. THRAP3_BCLAF1.
[Graphical view]
PANTHERiPTHR15268:SF4. PTHR15268:SF4. 1 hit.
PfamiPF15440. THRAP3_BCLAF1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K019-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRSNSRSHS SRSKSRSQSS SRSRSRSHSR KKRYSSRSRS RTYSRSRSRD
60 70 80 90 100
RIYSRDYRRD YRNNRGMRRP YGYRGRGRGY YQGGGGRYHR GGYRPVWNRR
110 120 130 140 150
HSRSPRRGRS RSRSPKRRSV SSQRSRSRSR RSYRSSRSPR SSSSRSSSPY
160 170 180 190 200
SKSPVSKRRG SQEKQTKKAE GEPQEESPLK SKSQEEPKDT FEHDPSESID
210 220 230 240 250
EFNKSATSGD IWPGLSAYDN SPRSPHSPSP IATPPSQSSS CSDAPMLSTV
260 270 280 290 300
HSAKNTPSQH SHSIQHSPER SGSGSVGNGS SRYSPSQNSP IHHIPSRRSP
310 320 330 340 350
AKTITPQNAP REESRGRSSF YPEGDQETAK TGKFLKRFTD EESRVFLLDR
360 370 380 390 400
GNIRDKEAPK EKGSEKGRAD GDWDDQEVLD YFSDKESAKQ KFHDSEGDDT
410 420 430 440 450
EETEDYRQFR KSVLADQGKS FATSSHRNTE EEGPKYKSKV SLKGNRESDG
460 470 480 490 500
FREEKNYKLK ETAYIVERPS TAKDKHKEED KGSDRITVKK EVQSPEQVKS
510 520 530 540 550
EKLKELFDYS PPLHKSLDAR EKSIFREESP LRIKMIASDS HRPEVKLKMA
560 570 580 590 600
PVPLDDSNRP ASLTKDRLLA STLVHSVKKE QEFRSIFDHI KLPQANKSTS
610 620 630 640 650
ESFIQHIVSL VHHVKEQYFK SPAVTLNERF TSYQKATEEH STRQKSPEIH
660 670 680 690 700
RRIDISPSAL RKHTRLAGEE RGFKEEIQKG DKKLRCDSAD LRHDIDRRRK
710 720 730 740 750
ERSKERGDSK GSRESSGSRK QEKTPKDYKE YKPYKDDSKH KGRERDHSRS
760 770 780 790 800
SSSSASPSSP SSREEKESKK EREEEFKTHH EMKDYSGFAG VSRPRGTFFR
810 820 830 840 850
IRGRGRARGV FAGTNTGPNN SNTTFQKRPK EEEWDPEYTP KSKKYFLHDD
860 870 880 890 900
RDDGVDYWAK RGRGRGTFQR GRGRFNFKKS GSSPKWTHDK YQGDGIVEDD
910
EETMENNEEK KDRRKEEKE
Length:919
Mass (Da):106,002
Last modified:May 24, 2004 - v2
Checksum:iF57E6327B29B8920
GO
Isoform 2 (identifier: Q8K019-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-36: Missing.

Show »
Length:917
Mass (Da):105,828
Checksum:i15B92D9FC8086C26
GO
Isoform 3 (identifier: Q8K019-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-36: Missing.
     740-750: HKGRERDHSRS → NVFVFYTAYGG
     751-919: Missing.

Show »
Length:748
Mass (Da):86,036
Checksum:iA4DFB462DE1069B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti616E → K in BAC37542 (PubMed:16141072).Curated1
Sequence conflicti667A → T in BAC37542 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01037235 – 36Missing in isoform 2 and isoform 3. 2 Publications2
Alternative sequenceiVSP_010373740 – 750HKGRERDHSRS → NVFVFYTAYGG in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_010374751 – 919Missing in isoform 3. 1 PublicationAdd BLAST169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011802 mRNA. Translation: BAB27851.1.
AK079110 mRNA. Translation: BAC37542.1.
AK088741 mRNA. Translation: BAC40542.1.
BC034300 mRNA. Translation: AAH34300.1.
AK129071 mRNA. Translation: BAC97881.1.
CCDSiCCDS35858.1. [Q8K019-1]
CCDS78798.1. [Q8K019-2]
RefSeqiNP_001020563.1. NM_001025392.1. [Q8K019-1]
NP_722482.1. NM_153787.2. [Q8K019-2]
UniGeneiMm.294783.

Genome annotation databases

EnsembliENSMUST00000043881; ENSMUSP00000043583; ENSMUSG00000037608. [Q8K019-1]
ENSMUST00000185800; ENSMUSP00000140623; ENSMUSG00000037608. [Q8K019-2]
ENSMUST00000190156; ENSMUSP00000140428; ENSMUSG00000037608. [Q8K019-3]
GeneIDi72567.
KEGGimmu:72567.
UCSCiuc007ent.1. mouse. [Q8K019-2]
uc007enu.1. mouse. [Q8K019-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011802 mRNA. Translation: BAB27851.1.
AK079110 mRNA. Translation: BAC37542.1.
AK088741 mRNA. Translation: BAC40542.1.
BC034300 mRNA. Translation: AAH34300.1.
AK129071 mRNA. Translation: BAC97881.1.
CCDSiCCDS35858.1. [Q8K019-1]
CCDS78798.1. [Q8K019-2]
RefSeqiNP_001020563.1. NM_001025392.1. [Q8K019-1]
NP_722482.1. NM_153787.2. [Q8K019-2]
UniGeneiMm.294783.

3D structure databases

ProteinModelPortaliQ8K019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215443. 6 interactors.
IntActiQ8K019. 4 interactors.
MINTiMINT-4089177.
STRINGi10090.ENSMUSP00000043583.

PTM databases

iPTMnetiQ8K019.
PhosphoSitePlusiQ8K019.

Proteomic databases

PaxDbiQ8K019.
PeptideAtlasiQ8K019.
PRIDEiQ8K019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043881; ENSMUSP00000043583; ENSMUSG00000037608. [Q8K019-1]
ENSMUST00000185800; ENSMUSP00000140623; ENSMUSG00000037608. [Q8K019-2]
ENSMUST00000190156; ENSMUSP00000140428; ENSMUSG00000037608. [Q8K019-3]
GeneIDi72567.
KEGGimmu:72567.
UCSCiuc007ent.1. mouse. [Q8K019-2]
uc007enu.1. mouse. [Q8K019-1]

Organism-specific databases

CTDi9774.
MGIiMGI:1917580. Bclaf1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IJCD. Eukaryota.
ENOG4110X68. LUCA.
GeneTreeiENSGT00530000063211.
HOGENOMiHOG000054209.
HOVERGENiHBG050681.
InParanoidiQ8K019.
KOiK13087.
OMAiDDSKHKS.
OrthoDBiEOG091G03HU.
PhylomeDBiQ8K019.
TreeFamiTF335939.

Miscellaneous databases

ChiTaRSiBclaf1. mouse.
PROiQ8K019.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037608.
CleanExiMM_BCLAF1.
ExpressionAtlasiQ8K019. baseline and differential.
GenevisibleiQ8K019. MM.

Family and domain databases

InterProiIPR026668. Bcl-2_assoc_TF1.
IPR029199. THRAP3_BCLAF1.
[Graphical view]
PANTHERiPTHR15268:SF4. PTHR15268:SF4. 1 hit.
PfamiPF15440. THRAP3_BCLAF1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCLF1_MOUSE
AccessioniPrimary (citable) accession number: Q8K019
Secondary accession number(s): Q8BNZ0, Q8C2E9, Q9CSW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.