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Protein

5-oxoprolinase

Gene

Oplah

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.By similarity

Catalytic activityi

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

GO - Molecular functioni

  1. 5-oxoprolinase (ATP-hydrolyzing) activity Source: MGI
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutathione metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_210980. Glutathione synthesis and recycling.
SABIO-RKQ8K010.

Names & Taxonomyi

Protein namesi
Recommended name:
5-oxoprolinase (EC:3.5.2.9)
Alternative name(s):
5-oxo-L-prolinase
Short name:
5-OPase
Pyroglutamase
Gene namesi
Name:Oplah
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1922725. Oplah.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128812885-oxoprolinasePRO_0000208578Add
BLAST

Proteomic databases

MaxQBiQ8K010.
PaxDbiQ8K010.
PRIDEiQ8K010.

PTM databases

PhosphoSiteiQ8K010.

Expressioni

Gene expression databases

BgeeiQ8K010.
CleanExiMM_OPLAH.
ExpressionAtlasiQ8K010. baseline and differential.
GenevestigatoriQ8K010.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ8K010. 1 interaction.
MINTiMINT-1855298.
STRINGi10090.ENSMUSP00000023222.

Family & Domainsi

Sequence similaritiesi

Belongs to the oxoprolinase family.Curated

Phylogenomic databases

eggNOGiCOG0146.
GeneTreeiENSGT00390000013463.
HOGENOMiHOG000047200.
HOVERGENiHBG007870.
InParanoidiQ8K010.
KOiK01469.
OMAiGNARMGY.
OrthoDBiEOG78M013.
PhylomeDBiQ8K010.
TreeFamiTF300520.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K010-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSPEERFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG
60 70 80 90 100
IRRILEQERG VLLPRGRPLD TSHIASIRMG TTVATNALLE RQGERVALLV
110 120 130 140 150
TRGFRDLLHI GTQARPDLFD LAVPMPEVLY EEVVEVDERV LLYRGEPGAG
160 170 180 190 200
SPVKGCTGDL LEIQQPVDLA ALRGKLEGLL TRGIHSLAVV LMHSYTWAQH
210 220 230 240 250
EQQVGTLARE LGFTHVSLSS EVMPMVRIVP RGHTACADAY LTPTIQRYVQ
260 270 280 290 300
GFRRGFQGQL KNVQVLFMRS DGGLAPMDAF SGSRAVLSGP AGGVVGYSTT
310 320 330 340 350
TYQLEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN
360 370 380 390 400
TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR
410 420 430 440 450
LLPASFPCIF GPGEDQPLSP EASRKALEAV AMEVNSFLAS GPCPASQLSL
460 470 480 490 500
EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA
510 520 530 540 550
RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLSYT PETFAQLDQR
560 570 580 590 600
LSRLEEQCVD ALQAQGFSRS QISTESFLHL RYQGTDCALM VSANQHPATT
610 620 630 640 650
CSPRAGDFGA AFVERYMREF GFIIPERSVV VDDVRVRGTG RSGLQLEETS
660 670 680 690 700
KIQSGPPHVE KVTQCYFEGG YQETPVYLLG ELGYGHQLQG PCLIIDNNST
710 720 730 740 750
ILVEPGCQAE VIETGDIRIS VGAEAPSMID TKLDPIQLSI FSHRFMSIAE
760 770 780 790 800
QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV
810 820 830 840 850
QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQSRPVFYV
860 870 880 890 900
ASRGHHADIG GITPGSMPPH STTLQQEGAV FLSFKLVQGG VFQEEAVTEA
910 920 930 940 950
LQAPGKISGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ
960 970 980 990 1000
AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSAKDHM DDGSPICLHV
1010 1020 1030 1040 1050
QINLNQGSAV FDFSGSGSEV FGNLNAPRAI TLSALIYCLR CLVGRDIPLN
1060 1070 1080 1090 1100
QGCLAPVQVI IPKGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACAAS
1110 1120 1130 1140 1150
QGCMNNVTLG NARMGYYETV AGGAGAGPGW HGRSGVHSHM TNTRITDPEI
1160 1170 1180 1190 1200
LESRYPVILR RFELRPGSGG RGRFRGGDGV VRELVFREEA LLSVLTERRA
1210 1220 1230 1240 1250
FQPYGLHGGE PGTRGLNLLI RKDGRTVNLG GKTSVTVYPG DAFCLHTPGG
1260 1270 1280
GGYGDPEDPA PPPGSPPLYP AFPERGSVYE YRRAQEAV
Length:1,288
Mass (Da):137,611
Last modified:October 1, 2002 - v1
Checksum:iDD6DDF24DF5AC390
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti902 – 9021Q → R in AAH25120. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025120 mRNA. Translation: AAH25120.1.
BC034522 mRNA. Translation: AAH34522.1.
CCDSiCCDS27565.1.
RefSeqiNP_694762.1. NM_153122.2.
XP_006521572.1. XM_006521509.1.
UniGeneiMm.322738.

Genome annotation databases

EnsembliENSMUST00000023222; ENSMUSP00000023222; ENSMUSG00000022562.
ENSMUST00000171340; ENSMUSP00000129100; ENSMUSG00000022562.
GeneIDi75475.
KEGGimmu:75475.
UCSCiuc007wjo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC025120 mRNA. Translation: AAH25120.1.
BC034522 mRNA. Translation: AAH34522.1.
CCDSiCCDS27565.1.
RefSeqiNP_694762.1. NM_153122.2.
XP_006521572.1. XM_006521509.1.
UniGeneiMm.322738.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K010. 1 interaction.
MINTiMINT-1855298.
STRINGi10090.ENSMUSP00000023222.

PTM databases

PhosphoSiteiQ8K010.

Proteomic databases

MaxQBiQ8K010.
PaxDbiQ8K010.
PRIDEiQ8K010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023222; ENSMUSP00000023222; ENSMUSG00000022562.
ENSMUST00000171340; ENSMUSP00000129100; ENSMUSG00000022562.
GeneIDi75475.
KEGGimmu:75475.
UCSCiuc007wjo.1. mouse.

Organism-specific databases

CTDi26873.
MGIiMGI:1922725. Oplah.

Phylogenomic databases

eggNOGiCOG0146.
GeneTreeiENSGT00390000013463.
HOGENOMiHOG000047200.
HOVERGENiHBG007870.
InParanoidiQ8K010.
KOiK01469.
OMAiGNARMGY.
OrthoDBiEOG78M013.
PhylomeDBiQ8K010.
TreeFamiTF300520.

Enzyme and pathway databases

ReactomeiREACT_210980. Glutathione synthesis and recycling.
SABIO-RKQ8K010.

Miscellaneous databases

ChiTaRSiOplah. mouse.
NextBioi343114.
PROiQ8K010.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K010.
CleanExiMM_OPLAH.
ExpressionAtlasiQ8K010. baseline and differential.
GenevestigatoriQ8K010.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiOPLA_MOUSE
AccessioniPrimary (citable) accession number: Q8K010
Secondary accession number(s): Q8R3K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: October 1, 2002
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.