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Protein

Splicing factor 45

Gene

Rbm17

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 45
Alternative name(s):
45 kDa-splicing factor
RNA-binding motif protein 17
Gene namesi
Name:Rbm17
Synonyms:Spf45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924188. Rbm17.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • protein complex Source: MGI
  • spliceosomal complex Source: UniProtKB-KW

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000819042 – 405Splicing factor 45Add BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei21N6-acetyllysineBy similarity1
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei41N6-acetyllysine; alternateCombined sources1
Cross-linki41Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki58Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei71PhosphothreonineBy similarity1
Modified residuei155PhosphoserineCombined sources1
Modified residuei169PhosphoserineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei237PhosphothreonineBy similarity1
Cross-linki256Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei266PhosphoserineBy similarity1
Cross-linki276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei295PhosphoserineBy similarity1
Modified residuei297PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8JZX4.
MaxQBiQ8JZX4.
PaxDbiQ8JZX4.
PeptideAtlasiQ8JZX4.
PRIDEiQ8JZX4.

PTM databases

iPTMnetiQ8JZX4.
PhosphoSitePlusiQ8JZX4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037197.
CleanExiMM_RBM17.
ExpressionAtlasiQ8JZX4. baseline and differential.
GenevisibleiQ8JZX4. MM.

Interactioni

Subunit structurei

Binds SXL. Associates with the spliceosome. Interacts with SF3B1, SF1 and U2AF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi218413. 1 interactor.
DIPiDIP-60651N.
IntActiQ8JZX4. 1 interactor.
MINTiMINT-4135305.
STRINGi10090.ENSMUSP00000041831.

Structurei

3D structure databases

ProteinModelPortaliQ8JZX4.
SMRiQ8JZX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini235 – 283G-patchPROSITE-ProRule annotationAdd BLAST49
Domaini310 – 389RRMAdd BLAST80

Phylogenomic databases

eggNOGiKOG1996. Eukaryota.
ENOG410XWJ5. LUCA.
GeneTreeiENSGT00790000123099.
HOGENOMiHOG000231726.
HOVERGENiHBG059363.
InParanoidiQ8JZX4.
KOiK12840.
OMAiAIPPPMY.
OrthoDBiEOG091G0I8J.
PhylomeDBiQ8JZX4.
TreeFamiTF313987.

Family and domain databases

CDDicd12647. RRM_UHM_SPF45. 1 hit.
InterProiView protein in InterPro
IPR000467. G_patch_dom.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR034653. SPF45_RRM.
IPR016967. Splicing_factor_SPF45.
PfamiView protein in Pfam
PF01585. G-patch. 1 hit.
PF00076. RRM_1. 1 hit.
PIRSFiPIRSF031066. Splicing_factor_SPF45. 1 hit.
SMARTiView protein in SMART
SM00443. G_patch. 1 hit.
SM00361. RRM_1. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50174. G_PATCH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JZX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV
60 70 80 90 100
LAPVIDLKRG GSSDDRQIAD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY
110 120 130 140 150
DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFSRR
160 170 180 190 200
PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP
210 220 230 240 250
RSQSSKAAIP PPVYEEPDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE
260 270 280 290 300
GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKGEAQD ASKKSDSNPL
310 320 330 340 350
TEILKCPTKV VLLRNMVGAG EVDEDLEVET KEECEKYGKV GKCVIFEIPG
360 370 380 390 400
APDDEAVRIF LEFERVESAI KAVVDLNGRY FGGRVVKACF YNLDKFRVLD

LAEQV
Length:405
Mass (Da):45,304
Last modified:October 1, 2002 - v1
Checksum:i2BCAE02BFA7AB8CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083384 mRNA. Translation: AAC64085.1.
AL831794 Genomic DNA. Translation: CAM13750.1.
BC034896 mRNA. Translation: AAH34896.1.
CCDSiCCDS15684.1.
RefSeqiNP_690037.1. NM_152824.1.
UniGeneiMm.182769.

Genome annotation databases

EnsembliENSMUST00000040314; ENSMUSP00000041831; ENSMUSG00000037197.
GeneIDi76938.
KEGGimmu:76938.
UCSCiuc008iio.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiSPF45_MOUSE
AccessioniPrimary (citable) accession number: Q8JZX4
Secondary accession number(s): A2AP41, O75939
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: October 1, 2002
Last modified: August 30, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot