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Q8JZR6

- S4A8_MOUSE

UniProt

Q8JZR6 - S4A8_MOUSE

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Protein

Electroneutral sodium bicarbonate exchanger 1

Gene

Slc4a8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3- exchange with a Na+:HCO3- stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3- from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3- into the cells. Also mediates lithium-dependent HCO3- cotransport. May be regulated by osmolarity.1 Publication

Enzyme regulationi

Activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).

GO - Molecular functioni

  1. inorganic anion exchanger activity Source: InterPro

GO - Biological processi

  1. sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Anion exchange, Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Electroneutral sodium bicarbonate exchanger 1
Alternative name(s):
Electroneutral Na+-driven Cl-HCO3 exchanger
Solute carrier family 4 member 8
k-NBC3
Gene namesi
Name:Slc4a8Imported
Synonyms:Kiaa0739
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1928745. Slc4a8.

Subcellular locationi

Membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: MGI
  3. neuronal cell body membrane Source: MGI
  4. neuron projection Source: MGI
  5. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10891089Electroneutral sodium bicarbonate exchanger 1PRO_0000328923Add
BLAST

Proteomic databases

MaxQBiQ8JZR6.
PaxDbiQ8JZR6.
PRIDEiQ8JZR6.

PTM databases

PhosphoSiteiQ8JZR6.

Expressioni

Tissue specificityi

Highly expressed in brain with lower levels in lung, kidney and heart. In the kidney, there is high expression in the inner medulla, localized to the inner medullary collecting duct. In the brain, there seems to be three transcripts each having a different expression pattern. The smaller 3kb transcript has highest expression levels in the thalamus and the largest 9.5kb transcript has highest levels in the substantia nigra. The middle transcript of 4.4kb, which is also the main transcript in kidney, is highly expressed in thalamus. Hence, the highest levels are observed in the thalamus, amygdala and caudate nucleus and very low expression was seen in the corpus callosum.1 Publication

Gene expression databases

BgeeiQ8JZR6.
ExpressionAtlasiQ8JZR6. baseline and differential.
GenevestigatoriQ8JZR6.

Structurei

3D structure databases

ProteinModelPortaliQ8JZR6.
SMRiQ8JZR6. Positions 115-396, 460-500.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 476476ExtracellularSequence AnalysisAdd
BLAST
Topological domaini498 – 5058CytoplasmicSequence Analysis
Topological domaini527 – 56337ExtracellularSequence AnalysisAdd
BLAST
Topological domaini585 – 5939CytoplasmicSequence Analysis
Topological domaini615 – 68571ExtracellularSequence AnalysisAdd
BLAST
Topological domaini707 – 72923CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini751 – 77626ExtracellularSequence AnalysisAdd
BLAST
Topological domaini798 – 82225CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini844 – 87936ExtracellularSequence AnalysisAdd
BLAST
Topological domaini901 – 9022CytoplasmicSequence Analysis
Topological domaini924 – 96037ExtracellularSequence AnalysisAdd
BLAST
Topological domaini982 – 1089108CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei477 – 49721HelicalSequence AnalysisAdd
BLAST
Transmembranei506 – 52621HelicalSequence AnalysisAdd
BLAST
Transmembranei564 – 58421HelicalSequence AnalysisAdd
BLAST
Transmembranei594 – 61421HelicalSequence AnalysisAdd
BLAST
Transmembranei686 – 70621HelicalSequence AnalysisAdd
BLAST
Transmembranei730 – 75021HelicalSequence AnalysisAdd
BLAST
Transmembranei777 – 79721HelicalSequence AnalysisAdd
BLAST
Transmembranei823 – 84321HelicalSequence AnalysisAdd
BLAST
Transmembranei880 – 90021HelicalSequence AnalysisAdd
BLAST
Transmembranei903 – 92321HelicalSequence AnalysisAdd
BLAST
Transmembranei961 – 98121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1021 – 10266Poly-GluSequence Analysis

Sequence similaritiesi

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]Sequence Analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268067.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
InParanoidiQ8JZR6.
KOiK13859.
OMAiVLNIHYE.
OrthoDBiEOG7TMZR0.
PhylomeDBiQ8JZR6.
TreeFamiTF313630.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 13 Publications (identifier: Q8JZR6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAGSNEPDG VLSYQRPDEE AVVDQGGTST ILNIHYEKEE LEGHRTLYVG
60 70 80 90 100
VRMPLGRQSH RHHRTHGQKH RRRGGRGKGA SQGEEGLEAL AHDTPSQRVQ
110 120 130 140 150
FILGTEEDEE HVPHELFTEL DEICMKEGED AEWKETARWL KFEEDVEDGG
160 170 180 190 200
ERWSKPYVAT LSLHSLFELR SCLINGSVLL DMRASSIEEI SDLILDQQEL
210 220 230 240 250
LRDLSDSVRV KVREALLKKH HHQNERRRNN LIPIVRSFAE VGKKQSDPHS
260 270 280 290 300
MDRDGQTVSP QSATNLEVKN GVNCEHSPVD LSKVDLHFMK KIPTGAEASN
310 320 330 340 350
VLVGEVDTLD RPIVAFVRLS PAVLLSGLTE VPIPTRFLFI LLGPVGKGQQ
360 370 380 390 400
YHEIGRSMAT IMTDEIFHDV AYKAKERDDL LAGIDEFLDQ VTVLPPGEWD
410 420 430 440 450
PSIRIEPPKN VPSQEKRKMP GVPNGNVCHI EPEPHGGHSG PELERTGRLF
460 470 480 490 500
GGLVLDVKRK APWYWSDYRD ALSLQCLASF LFLYCACMSP VITFGGLLGE
510 520 530 540 550
ATEGRISAIE SLFGASMTGI AYSLFAGQPL TILGSTGPVL VFEKILFKFC
560 570 580 590 600
KDYALSYLSL RALIGLWTAF LCIVLVATDA SSLVCYITRF TEEAFASLIC
610 620 630 640 650
IIFIYEAIEK LIHLAETYPI HMHSQLDHLS LYYCRCVLPE NPNNHTLQYW
660 670 680 690 700
KDHNILAAEV NWANLTVSEC QEMHGEFMGS ACGHHGPYTP DVLFWSCILF
710 720 730 740 750
FATFIVSSTL KTFKTSRYFP TRVRSMVSDF AVFLTIFTMV VLDFLIGVPS
760 770 780 790 800
PKLQVPNVFK PTRDDRGWFI NPIGPNPWWT VIAAIIPALL CTILIFMDQQ
810 820 830 840 850
ITAVIINRKE HKLKKGCGYH LDLLMVAVML GVCSIMGLPW FVAATVLSIT
860 870 880 890 900
HVNSLKLESE CSAPGEQPKF LGIREQRVTG LMIFVLMGCS VFMTAVLKFI
910 920 930 940 950
PMPVLYGVFL YMGVSSLQGI QFFDRLKLFG MPAKHQPDFI YLRHVPLRKV
960 970 980 990 1000
HLFTLVQLTC LVLLWVIKAS PAAIVFPMMV LALVFVRKVM DLCFSKRELS
1010 1020 1030 1040 1050
WLDDLMPESK KKKLDDAKKK EEEEAEKMLD IGGDKFPLES RKLLSSPGKS
1060 1070 1080
SSFRCDPSEI NISDEMPKTT VWKALSINSG NTKEKSPFN
Length:1,089
Mass (Da):122,421
Last modified:October 1, 2002 - v1
Checksum:iCFAE127D3853DDD8
GO
Isoform 21 Publication (identifier: Q8JZR6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Show »
Length:1,037
Mass (Da):116,681
Checksum:i81818C80B335F19B
GO

Sequence cautioni

The sequence BAD32292.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191K → E in BAE42561. (PubMed:16141072)Curated
Sequence conflicti258 – 2581V → M in AAF61705. (PubMed:11260402)Curated
Sequence conflicti291 – 2911K → R in BAE42561. (PubMed:16141072)Curated
Sequence conflicti644 – 6441N → S in BAC30341. (PubMed:16141072)Curated
Sequence conflicti707 – 7071S → P in AAF61705. (PubMed:11260402)Curated
Sequence conflicti949 – 9491K → E in BAE42561. (PubMed:16141072)Curated
Sequence conflicti1015 – 10151D → G in BAC30341. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5252Missing in isoform 2. 1 PublicationVSP_052769Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF224508 mRNA. Translation: AAF61705.1.
AK039404 mRNA. Translation: BAC30341.1.
AK171610 mRNA. Translation: BAE42561.1.
AK173014 mRNA. Translation: BAD32292.1. Different initiation.
BC030388 mRNA. Translation: AAH30388.1.
CCDSiCCDS37214.1. [Q8JZR6-1]
RefSeqiNP_067505.2. NM_021530.2. [Q8JZR6-1]
XP_006521288.1. XM_006521225.1. [Q8JZR6-2]
XP_006521289.1. XM_006521226.1. [Q8JZR6-2]
UniGeneiMm.209856.

Genome annotation databases

EnsembliENSMUST00000023776; ENSMUSP00000023776; ENSMUSG00000023032. [Q8JZR6-1]
ENSMUST00000162049; ENSMUSP00000125090; ENSMUSG00000023032. [Q8JZR6-2]
GeneIDi59033.
KEGGimmu:59033.
UCSCiuc007xse.1. mouse. [Q8JZR6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF224508 mRNA. Translation: AAF61705.1 .
AK039404 mRNA. Translation: BAC30341.1 .
AK171610 mRNA. Translation: BAE42561.1 .
AK173014 mRNA. Translation: BAD32292.1 . Different initiation.
BC030388 mRNA. Translation: AAH30388.1 .
CCDSi CCDS37214.1. [Q8JZR6-1 ]
RefSeqi NP_067505.2. NM_021530.2. [Q8JZR6-1 ]
XP_006521288.1. XM_006521225.1. [Q8JZR6-2 ]
XP_006521289.1. XM_006521226.1. [Q8JZR6-2 ]
UniGenei Mm.209856.

3D structure databases

ProteinModelPortali Q8JZR6.
SMRi Q8JZR6. Positions 115-396, 460-500.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8JZR6.

Proteomic databases

MaxQBi Q8JZR6.
PaxDbi Q8JZR6.
PRIDEi Q8JZR6.

Protocols and materials databases

DNASUi 59033.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023776 ; ENSMUSP00000023776 ; ENSMUSG00000023032 . [Q8JZR6-1 ]
ENSMUST00000162049 ; ENSMUSP00000125090 ; ENSMUSG00000023032 . [Q8JZR6-2 ]
GeneIDi 59033.
KEGGi mmu:59033.
UCSCi uc007xse.1. mouse. [Q8JZR6-1 ]

Organism-specific databases

CTDi 9498.
MGIi MGI:1928745. Slc4a8.
Rougei Search...

Phylogenomic databases

eggNOGi NOG268067.
GeneTreei ENSGT00760000119021.
HOGENOMi HOG000280684.
HOVERGENi HBG004326.
InParanoidi Q8JZR6.
KOi K13859.
OMAi VLNIHYE.
OrthoDBi EOG7TMZR0.
PhylomeDBi Q8JZR6.
TreeFami TF313630.

Miscellaneous databases

NextBioi 314630.
PROi Q8JZR6.
SOURCEi Search...

Gene expression databases

Bgeei Q8JZR6.
ExpressionAtlasi Q8JZR6. baseline and differential.
Genevestigatori Q8JZR6.

Family and domain databases

Gene3Di 3.40.1100.10. 1 hit.
InterProi IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view ]
PANTHERi PTHR11453. PTHR11453. 1 hit.
Pfami PF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view ]
PRINTSi PR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMi SSF55804. SSF55804. 1 hit.
TIGRFAMsi TIGR00834. ae. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution."
    Wang Z., Conforti L., Petrovic S., Amlal H., Burnham C.E., Soleimani M.
    Kidney Int. 59:1405-1414(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INHIBITION.
    Tissue: Kidney inner medullaImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6JImported.
    Tissue: Dendritic cell1 Publication and Spinal cordImported.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brainImported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/61 Publication.
    Tissue: RetinaImported.

Entry informationi

Entry nameiS4A8_MOUSE
AccessioniPrimary (citable) accession number: Q8JZR6
Secondary accession number(s): Q3TAV7
, Q6A004, Q8BYI7, Q9JKV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3