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Q8JZR6

- S4A8_MOUSE

UniProt

Q8JZR6 - S4A8_MOUSE

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Protein
Electroneutral sodium bicarbonate exchanger 1
Gene
Slc4a8, Kiaa0739
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3- exchange with a Na+:HCO3- stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3- from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3- into the cells. Also mediates lithium-dependent HCO3- cotransport. May be regulated by osmolarity.By similarity1 Publication

Enzyme regulationi

Activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).

GO - Molecular functioni

  1. inorganic anion exchanger activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Anion exchange, Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Electroneutral sodium bicarbonate exchanger 1
Alternative name(s):
Electroneutral Na+-driven Cl-HCO3 exchanger
Solute carrier family 4 member 8
k-NBC3
Gene namesi
Name:Slc4a8
Synonyms:Kiaa0739
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1928745. Slc4a8.

Subcellular locationi

Membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 476476Extracellular Reviewed prediction
Add
BLAST
Transmembranei477 – 49721Helical; Reviewed prediction
Add
BLAST
Topological domaini498 – 5058Cytoplasmic Reviewed prediction
Transmembranei506 – 52621Helical; Reviewed prediction
Add
BLAST
Topological domaini527 – 56337Extracellular Reviewed prediction
Add
BLAST
Transmembranei564 – 58421Helical; Reviewed prediction
Add
BLAST
Topological domaini585 – 5939Cytoplasmic Reviewed prediction
Transmembranei594 – 61421Helical; Reviewed prediction
Add
BLAST
Topological domaini615 – 68571Extracellular Reviewed prediction
Add
BLAST
Transmembranei686 – 70621Helical; Reviewed prediction
Add
BLAST
Topological domaini707 – 72923Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei730 – 75021Helical; Reviewed prediction
Add
BLAST
Topological domaini751 – 77626Extracellular Reviewed prediction
Add
BLAST
Transmembranei777 – 79721Helical; Reviewed prediction
Add
BLAST
Topological domaini798 – 82225Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei823 – 84321Helical; Reviewed prediction
Add
BLAST
Topological domaini844 – 87936Extracellular Reviewed prediction
Add
BLAST
Transmembranei880 – 90021Helical; Reviewed prediction
Add
BLAST
Topological domaini901 – 9022Cytoplasmic Reviewed prediction
Transmembranei903 – 92321Helical; Reviewed prediction
Add
BLAST
Topological domaini924 – 96037Extracellular Reviewed prediction
Add
BLAST
Transmembranei961 – 98121Helical; Reviewed prediction
Add
BLAST
Topological domaini982 – 1089108Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: MGI
  3. neuron projection Source: MGI
  4. neuronal cell body membrane Source: MGI
  5. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10891089Electroneutral sodium bicarbonate exchanger 1
PRO_0000328923Add
BLAST

Proteomic databases

PaxDbiQ8JZR6.
PRIDEiQ8JZR6.

PTM databases

PhosphoSiteiQ8JZR6.

Expressioni

Tissue specificityi

Highly expressed in brain with lower levels in lung, kidney and heart. In the kidney, there is high expression in the inner medulla, localized to the inner medullary collecting duct. In the brain, there seems to be three transcripts each having a different expression pattern. The smaller 3kb transcript has highest expression levels in the thalamus and the largest 9.5kb transcript has highest levels in the substantia nigra. The middle transcript of 4.4kb, which is also the main transcript in kidney, is highly expressed in thalamus. Hence, the highest levels are observed in the thalamus, amygdala and caudate nucleus and very low expression was seen in the corpus callosum.1 Publication

Gene expression databases

ArrayExpressiQ8JZR6.
BgeeiQ8JZR6.
GenevestigatoriQ8JZR6.

Structurei

3D structure databases

ProteinModelPortaliQ8JZR6.
SMRiQ8JZR6. Positions 115-396, 460-500.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1021 – 10266Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268067.
GeneTreeiENSGT00560000076851.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
InParanoidiQ8JZR6.
KOiK13859.
OMAiVLNIHYE.
OrthoDBiEOG7TMZR0.
PhylomeDBiQ8JZR6.
TreeFamiTF313630.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 13 Publications (identifier: Q8JZR6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPAGSNEPDG VLSYQRPDEE AVVDQGGTST ILNIHYEKEE LEGHRTLYVG     50
VRMPLGRQSH RHHRTHGQKH RRRGGRGKGA SQGEEGLEAL AHDTPSQRVQ 100
FILGTEEDEE HVPHELFTEL DEICMKEGED AEWKETARWL KFEEDVEDGG 150
ERWSKPYVAT LSLHSLFELR SCLINGSVLL DMRASSIEEI SDLILDQQEL 200
LRDLSDSVRV KVREALLKKH HHQNERRRNN LIPIVRSFAE VGKKQSDPHS 250
MDRDGQTVSP QSATNLEVKN GVNCEHSPVD LSKVDLHFMK KIPTGAEASN 300
VLVGEVDTLD RPIVAFVRLS PAVLLSGLTE VPIPTRFLFI LLGPVGKGQQ 350
YHEIGRSMAT IMTDEIFHDV AYKAKERDDL LAGIDEFLDQ VTVLPPGEWD 400
PSIRIEPPKN VPSQEKRKMP GVPNGNVCHI EPEPHGGHSG PELERTGRLF 450
GGLVLDVKRK APWYWSDYRD ALSLQCLASF LFLYCACMSP VITFGGLLGE 500
ATEGRISAIE SLFGASMTGI AYSLFAGQPL TILGSTGPVL VFEKILFKFC 550
KDYALSYLSL RALIGLWTAF LCIVLVATDA SSLVCYITRF TEEAFASLIC 600
IIFIYEAIEK LIHLAETYPI HMHSQLDHLS LYYCRCVLPE NPNNHTLQYW 650
KDHNILAAEV NWANLTVSEC QEMHGEFMGS ACGHHGPYTP DVLFWSCILF 700
FATFIVSSTL KTFKTSRYFP TRVRSMVSDF AVFLTIFTMV VLDFLIGVPS 750
PKLQVPNVFK PTRDDRGWFI NPIGPNPWWT VIAAIIPALL CTILIFMDQQ 800
ITAVIINRKE HKLKKGCGYH LDLLMVAVML GVCSIMGLPW FVAATVLSIT 850
HVNSLKLESE CSAPGEQPKF LGIREQRVTG LMIFVLMGCS VFMTAVLKFI 900
PMPVLYGVFL YMGVSSLQGI QFFDRLKLFG MPAKHQPDFI YLRHVPLRKV 950
HLFTLVQLTC LVLLWVIKAS PAAIVFPMMV LALVFVRKVM DLCFSKRELS 1000
WLDDLMPESK KKKLDDAKKK EEEEAEKMLD IGGDKFPLES RKLLSSPGKS 1050
SSFRCDPSEI NISDEMPKTT VWKALSINSG NTKEKSPFN 1089
Length:1,089
Mass (Da):122,421
Last modified:October 1, 2002 - v1
Checksum:iCFAE127D3853DDD8
GO
Isoform 21 Publication (identifier: Q8JZR6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Show »
Length:1,037
Mass (Da):116,681
Checksum:i81818C80B335F19B
GO

Sequence cautioni

The sequence BAD32292.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5252Missing in isoform 2. 1 Publication
VSP_052769Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191K → E in BAE42561. 1 Publication
Sequence conflicti258 – 2581V → M in AAF61705. 1 Publication
Sequence conflicti291 – 2911K → R in BAE42561. 1 Publication
Sequence conflicti644 – 6441N → S in BAC30341. 1 Publication
Sequence conflicti707 – 7071S → P in AAF61705. 1 Publication
Sequence conflicti949 – 9491K → E in BAE42561. 1 Publication
Sequence conflicti1015 – 10151D → G in BAC30341. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF224508 mRNA. Translation: AAF61705.1.
AK039404 mRNA. Translation: BAC30341.1.
AK171610 mRNA. Translation: BAE42561.1.
AK173014 mRNA. Translation: BAD32292.1. Different initiation.
BC030388 mRNA. Translation: AAH30388.1.
CCDSiCCDS37214.1. [Q8JZR6-1]
RefSeqiNP_067505.2. NM_021530.2. [Q8JZR6-1]
XP_006521288.1. XM_006521225.1. [Q8JZR6-2]
XP_006521289.1. XM_006521226.1. [Q8JZR6-2]
UniGeneiMm.209856.

Genome annotation databases

EnsembliENSMUST00000023776; ENSMUSP00000023776; ENSMUSG00000023032. [Q8JZR6-1]
ENSMUST00000162049; ENSMUSP00000125090; ENSMUSG00000023032. [Q8JZR6-2]
GeneIDi59033.
KEGGimmu:59033.
UCSCiuc007xse.1. mouse. [Q8JZR6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF224508 mRNA. Translation: AAF61705.1 .
AK039404 mRNA. Translation: BAC30341.1 .
AK171610 mRNA. Translation: BAE42561.1 .
AK173014 mRNA. Translation: BAD32292.1 . Different initiation.
BC030388 mRNA. Translation: AAH30388.1 .
CCDSi CCDS37214.1. [Q8JZR6-1 ]
RefSeqi NP_067505.2. NM_021530.2. [Q8JZR6-1 ]
XP_006521288.1. XM_006521225.1. [Q8JZR6-2 ]
XP_006521289.1. XM_006521226.1. [Q8JZR6-2 ]
UniGenei Mm.209856.

3D structure databases

ProteinModelPortali Q8JZR6.
SMRi Q8JZR6. Positions 115-396, 460-500.
ModBasei Search...

PTM databases

PhosphoSitei Q8JZR6.

Proteomic databases

PaxDbi Q8JZR6.
PRIDEi Q8JZR6.

Protocols and materials databases

DNASUi 59033.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023776 ; ENSMUSP00000023776 ; ENSMUSG00000023032 . [Q8JZR6-1 ]
ENSMUST00000162049 ; ENSMUSP00000125090 ; ENSMUSG00000023032 . [Q8JZR6-2 ]
GeneIDi 59033.
KEGGi mmu:59033.
UCSCi uc007xse.1. mouse. [Q8JZR6-1 ]

Organism-specific databases

CTDi 9498.
MGIi MGI:1928745. Slc4a8.
Rougei Search...

Phylogenomic databases

eggNOGi NOG268067.
GeneTreei ENSGT00560000076851.
HOGENOMi HOG000280684.
HOVERGENi HBG004326.
InParanoidi Q8JZR6.
KOi K13859.
OMAi VLNIHYE.
OrthoDBi EOG7TMZR0.
PhylomeDBi Q8JZR6.
TreeFami TF313630.

Miscellaneous databases

NextBioi 314630.
PROi Q8JZR6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8JZR6.
Bgeei Q8JZR6.
Genevestigatori Q8JZR6.

Family and domain databases

Gene3Di 3.40.1100.10. 1 hit.
InterProi IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view ]
PANTHERi PTHR11453. PTHR11453. 1 hit.
Pfami PF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view ]
PRINTSi PR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMi SSF55804. SSF55804. 1 hit.
TIGRFAMsi TIGR00834. ae. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution."
    Wang Z., Conforti L., Petrovic S., Amlal H., Burnham C.E., Soleimani M.
    Kidney Int. 59:1405-1414(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INHIBITION.
    Tissue: Kidney inner medulla.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Dendritic cell and Spinal cord.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiS4A8_MOUSE
AccessioniPrimary (citable) accession number: Q8JZR6
Secondary accession number(s): Q3TAV7
, Q6A004, Q8BYI7, Q9JKV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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