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Q8JZR6

- S4A8_MOUSE

UniProt

Q8JZR6 - S4A8_MOUSE

Protein

Electroneutral sodium bicarbonate exchanger 1

Gene

Slc4a8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3- exchange with a Na+:HCO3- stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3- from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3- into the cells. Also mediates lithium-dependent HCO3- cotransport. May be regulated by osmolarity.1 Publication

    Enzyme regulationi

    Activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).

    GO - Molecular functioni

    1. inorganic anion exchanger activity Source: InterPro

    GO - Biological processi

    1. sodium ion transport Source: UniProtKB-KW

    Keywords - Biological processi

    Anion exchange, Antiport, Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Electroneutral sodium bicarbonate exchanger 1
    Alternative name(s):
    Electroneutral Na+-driven Cl-HCO3 exchanger
    Solute carrier family 4 member 8
    k-NBC3
    Gene namesi
    Name:Slc4a8Imported
    Synonyms:Kiaa0739
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1928745. Slc4a8.

    Subcellular locationi

    Membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane Source: MGI
    3. neuronal cell body membrane Source: MGI
    4. neuron projection Source: MGI
    5. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10891089Electroneutral sodium bicarbonate exchanger 1PRO_0000328923Add
    BLAST

    Proteomic databases

    PaxDbiQ8JZR6.
    PRIDEiQ8JZR6.

    PTM databases

    PhosphoSiteiQ8JZR6.

    Expressioni

    Tissue specificityi

    Highly expressed in brain with lower levels in lung, kidney and heart. In the kidney, there is high expression in the inner medulla, localized to the inner medullary collecting duct. In the brain, there seems to be three transcripts each having a different expression pattern. The smaller 3kb transcript has highest expression levels in the thalamus and the largest 9.5kb transcript has highest levels in the substantia nigra. The middle transcript of 4.4kb, which is also the main transcript in kidney, is highly expressed in thalamus. Hence, the highest levels are observed in the thalamus, amygdala and caudate nucleus and very low expression was seen in the corpus callosum.1 Publication

    Gene expression databases

    ArrayExpressiQ8JZR6.
    BgeeiQ8JZR6.
    GenevestigatoriQ8JZR6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8JZR6.
    SMRiQ8JZR6. Positions 115-396, 460-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 476476ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini498 – 5058CytoplasmicSequence Analysis
    Topological domaini527 – 56337ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini585 – 5939CytoplasmicSequence Analysis
    Topological domaini615 – 68571ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini707 – 72923CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini751 – 77626ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini798 – 82225CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini844 – 87936ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini901 – 9022CytoplasmicSequence Analysis
    Topological domaini924 – 96037ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini982 – 1089108CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei477 – 49721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei506 – 52621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei564 – 58421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei594 – 61421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei686 – 70621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei730 – 75021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei777 – 79721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei823 – 84321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei880 – 90021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei903 – 92321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei961 – 98121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1021 – 10266Poly-GluSequence Analysis

    Sequence similaritiesi

    Belongs to the anion exchanger (TC 2.A.31) family. [View classification]Sequence Analysis

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268067.
    GeneTreeiENSGT00560000076851.
    HOGENOMiHOG000280684.
    HOVERGENiHBG004326.
    InParanoidiQ8JZR6.
    KOiK13859.
    OMAiVLNIHYE.
    OrthoDBiEOG7TMZR0.
    PhylomeDBiQ8JZR6.
    TreeFamiTF313630.

    Family and domain databases

    Gene3Di3.40.1100.10. 1 hit.
    InterProiIPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR003024. Na/HCO3_transpt.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view]
    PANTHERiPTHR11453. PTHR11453. 1 hit.
    PfamiPF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 1 hit.
    [Graphical view]
    PRINTSiPR01231. HCO3TRNSPORT.
    PR01232. NAHCO3TRSPRT.
    SUPFAMiSSF55804. SSF55804. 1 hit.
    TIGRFAMsiTIGR00834. ae. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 13 Publications (identifier: Q8JZR6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPAGSNEPDG VLSYQRPDEE AVVDQGGTST ILNIHYEKEE LEGHRTLYVG     50
    VRMPLGRQSH RHHRTHGQKH RRRGGRGKGA SQGEEGLEAL AHDTPSQRVQ 100
    FILGTEEDEE HVPHELFTEL DEICMKEGED AEWKETARWL KFEEDVEDGG 150
    ERWSKPYVAT LSLHSLFELR SCLINGSVLL DMRASSIEEI SDLILDQQEL 200
    LRDLSDSVRV KVREALLKKH HHQNERRRNN LIPIVRSFAE VGKKQSDPHS 250
    MDRDGQTVSP QSATNLEVKN GVNCEHSPVD LSKVDLHFMK KIPTGAEASN 300
    VLVGEVDTLD RPIVAFVRLS PAVLLSGLTE VPIPTRFLFI LLGPVGKGQQ 350
    YHEIGRSMAT IMTDEIFHDV AYKAKERDDL LAGIDEFLDQ VTVLPPGEWD 400
    PSIRIEPPKN VPSQEKRKMP GVPNGNVCHI EPEPHGGHSG PELERTGRLF 450
    GGLVLDVKRK APWYWSDYRD ALSLQCLASF LFLYCACMSP VITFGGLLGE 500
    ATEGRISAIE SLFGASMTGI AYSLFAGQPL TILGSTGPVL VFEKILFKFC 550
    KDYALSYLSL RALIGLWTAF LCIVLVATDA SSLVCYITRF TEEAFASLIC 600
    IIFIYEAIEK LIHLAETYPI HMHSQLDHLS LYYCRCVLPE NPNNHTLQYW 650
    KDHNILAAEV NWANLTVSEC QEMHGEFMGS ACGHHGPYTP DVLFWSCILF 700
    FATFIVSSTL KTFKTSRYFP TRVRSMVSDF AVFLTIFTMV VLDFLIGVPS 750
    PKLQVPNVFK PTRDDRGWFI NPIGPNPWWT VIAAIIPALL CTILIFMDQQ 800
    ITAVIINRKE HKLKKGCGYH LDLLMVAVML GVCSIMGLPW FVAATVLSIT 850
    HVNSLKLESE CSAPGEQPKF LGIREQRVTG LMIFVLMGCS VFMTAVLKFI 900
    PMPVLYGVFL YMGVSSLQGI QFFDRLKLFG MPAKHQPDFI YLRHVPLRKV 950
    HLFTLVQLTC LVLLWVIKAS PAAIVFPMMV LALVFVRKVM DLCFSKRELS 1000
    WLDDLMPESK KKKLDDAKKK EEEEAEKMLD IGGDKFPLES RKLLSSPGKS 1050
    SSFRCDPSEI NISDEMPKTT VWKALSINSG NTKEKSPFN 1089
    Length:1,089
    Mass (Da):122,421
    Last modified:October 1, 2002 - v1
    Checksum:iCFAE127D3853DDD8
    GO
    Isoform 21 Publication (identifier: Q8JZR6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-52: Missing.

    Show »
    Length:1,037
    Mass (Da):116,681
    Checksum:i81818C80B335F19B
    GO

    Sequence cautioni

    The sequence BAD32292.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2191K → E in BAE42561. (PubMed:16141072)Curated
    Sequence conflicti258 – 2581V → M in AAF61705. (PubMed:11260402)Curated
    Sequence conflicti291 – 2911K → R in BAE42561. (PubMed:16141072)Curated
    Sequence conflicti644 – 6441N → S in BAC30341. (PubMed:16141072)Curated
    Sequence conflicti707 – 7071S → P in AAF61705. (PubMed:11260402)Curated
    Sequence conflicti949 – 9491K → E in BAE42561. (PubMed:16141072)Curated
    Sequence conflicti1015 – 10151D → G in BAC30341. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5252Missing in isoform 2. 1 PublicationVSP_052769Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF224508 mRNA. Translation: AAF61705.1.
    AK039404 mRNA. Translation: BAC30341.1.
    AK171610 mRNA. Translation: BAE42561.1.
    AK173014 mRNA. Translation: BAD32292.1. Different initiation.
    BC030388 mRNA. Translation: AAH30388.1.
    CCDSiCCDS37214.1. [Q8JZR6-1]
    RefSeqiNP_067505.2. NM_021530.2. [Q8JZR6-1]
    XP_006521288.1. XM_006521225.1. [Q8JZR6-2]
    XP_006521289.1. XM_006521226.1. [Q8JZR6-2]
    UniGeneiMm.209856.

    Genome annotation databases

    EnsembliENSMUST00000023776; ENSMUSP00000023776; ENSMUSG00000023032. [Q8JZR6-1]
    ENSMUST00000162049; ENSMUSP00000125090; ENSMUSG00000023032. [Q8JZR6-2]
    GeneIDi59033.
    KEGGimmu:59033.
    UCSCiuc007xse.1. mouse. [Q8JZR6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF224508 mRNA. Translation: AAF61705.1 .
    AK039404 mRNA. Translation: BAC30341.1 .
    AK171610 mRNA. Translation: BAE42561.1 .
    AK173014 mRNA. Translation: BAD32292.1 . Different initiation.
    BC030388 mRNA. Translation: AAH30388.1 .
    CCDSi CCDS37214.1. [Q8JZR6-1 ]
    RefSeqi NP_067505.2. NM_021530.2. [Q8JZR6-1 ]
    XP_006521288.1. XM_006521225.1. [Q8JZR6-2 ]
    XP_006521289.1. XM_006521226.1. [Q8JZR6-2 ]
    UniGenei Mm.209856.

    3D structure databases

    ProteinModelPortali Q8JZR6.
    SMRi Q8JZR6. Positions 115-396, 460-500.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8JZR6.

    Proteomic databases

    PaxDbi Q8JZR6.
    PRIDEi Q8JZR6.

    Protocols and materials databases

    DNASUi 59033.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023776 ; ENSMUSP00000023776 ; ENSMUSG00000023032 . [Q8JZR6-1 ]
    ENSMUST00000162049 ; ENSMUSP00000125090 ; ENSMUSG00000023032 . [Q8JZR6-2 ]
    GeneIDi 59033.
    KEGGi mmu:59033.
    UCSCi uc007xse.1. mouse. [Q8JZR6-1 ]

    Organism-specific databases

    CTDi 9498.
    MGIi MGI:1928745. Slc4a8.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG268067.
    GeneTreei ENSGT00560000076851.
    HOGENOMi HOG000280684.
    HOVERGENi HBG004326.
    InParanoidi Q8JZR6.
    KOi K13859.
    OMAi VLNIHYE.
    OrthoDBi EOG7TMZR0.
    PhylomeDBi Q8JZR6.
    TreeFami TF313630.

    Miscellaneous databases

    NextBioi 314630.
    PROi Q8JZR6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8JZR6.
    Bgeei Q8JZR6.
    Genevestigatori Q8JZR6.

    Family and domain databases

    Gene3Di 3.40.1100.10. 1 hit.
    InterProi IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR003024. Na/HCO3_transpt.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view ]
    PANTHERi PTHR11453. PTHR11453. 1 hit.
    Pfami PF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 1 hit.
    [Graphical view ]
    PRINTSi PR01231. HCO3TRNSPORT.
    PR01232. NAHCO3TRSPRT.
    SUPFAMi SSF55804. SSF55804. 1 hit.
    TIGRFAMsi TIGR00834. ae. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution."
      Wang Z., Conforti L., Petrovic S., Amlal H., Burnham C.E., Soleimani M.
      Kidney Int. 59:1405-1414(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INHIBITION.
      Tissue: Kidney inner medullaImported.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6JImported.
      Tissue: Dendritic cell1 Publication and Spinal cordImported.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Fetal brainImported.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/61 Publication.
      Tissue: RetinaImported.

    Entry informationi

    Entry nameiS4A8_MOUSE
    AccessioniPrimary (citable) accession number: Q8JZR6
    Secondary accession number(s): Q3TAV7
    , Q6A004, Q8BYI7, Q9JKV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3