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Q8JZR0 (ACSL5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase 5

EC=6.2.1.3
Alternative name(s):
Long-chain acyl-CoA synthetase 5
Short name=LACS 5
Gene names
Name:Acsl5
Synonyms:Facl5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length683 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage By similarity. It was suggested that it may also stimulate fatty acid oxidation By similarity. At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL By similarity. May have a role in the survival of glioma cells By similarity. Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids By similarity.

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Subcellular location

Mitochondrion By similarity. Endoplasmic reticulum By similarity. Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 683683Long-chain-fatty-acid--CoA ligase 5
PRO_0000193113

Regions

Transmembrane12 – 3221Helical; Signal-anchor for type III membrane protein; Potential
Topological domain33 – 683651Cytoplasmic Potential

Amino acid modifications

Modified residue3611N6-acetyllysine Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8JZR0 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 734A1A5878741D70

FASTA68376,206
        10         20         30         40         50         60 
MLFIFNFLFS PLPTPALICL LTFGTAIFLW LINRPQPVLP LIDLDNQSVG IEGGARRGAF 

        70         80         90        100        110        120 
QKNNDLILYY FSDAKTLYEN FQRGLAVSDN GPCLGYRKPN QPYKWISYKQ VSDRAEYLGS 

       130        140        150        160        170        180 
CLLHKGYKSS QDQFVGIFAQ NRPEWVISEL ACYTYSMVAV PLYDTLGTEA IIFVINRADI 

       190        200        210        220        230        240 
PVVICDTPQK ATMLVENVEK GLTPGLKTII LMDPFDDDLM KRGEKCGVEM LSLHDAENIG 

       250        260        270        280        290        300 
KENFKKPVPP KPEDLSVICF TSGTTGDPKG AMLTHENVVS NMAAFLKFLE PIFQPTSDDV 

       310        320        330        340        350        360 
TISYLPLAHM FERLVQGILF SCGGKIGFFQ GDIRLLPDDM KALKPTVFPT VPRLLNRVYD 

       370        380        390        400        410        420 
KVQNEAKTPL KKFLLNLAII SKFNEVKNGI IRRDSLWDKL VFSKIQGSLG GKVRLMITGA 

       430        440        450        460        470        480 
APISTPVLTF FRAAMGCWVF EAYGQTECTG GCSITSPGDW TAGHVGTPVA CNFVKLEDVA 

       490        500        510        520        530        540 
DMNYFSVNNE GEICIKGNNV FKGYLKDPEK TQEVLDKDGW LHTGDIGRWL PNGTLKIVDR 

       550        560        570        580        590        600 
KKNIFKLAQG EYIAPEKIEN VYSRSRPVLQ VFVHGESLRS FLIGVVVPDP DSLPSFAAKI 

       610        620        630        640        650        660 
GVKGSFEELC KNQCVKEAIL EDLQKIGKEG GLKSFEQVKS IFVHPEPFTI ENGLLTPTLK 

       670        680 
AKRVELAKFF QTQIKSLYES IEE 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[2]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC031544 mRNA. Translation: AAH31544.1.
PIRS49487.
RefSeqNP_082252.1. NM_027976.2.
XP_006527241.1. XM_006527178.1.
UniGeneMm.292056.

3D structure databases

ProteinModelPortalQ8JZR0.
SMRQ8JZR0. Positions 76-674.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8JZR0. 2 interactions.
MINTMINT-1862123.

PTM databases

PhosphoSiteQ8JZR0.

Proteomic databases

PaxDbQ8JZR0.
PRIDEQ8JZR0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043150; ENSMUSP00000046585; ENSMUSG00000024981.
GeneID433256.
KEGGmmu:433256.
UCSCuc008hxp.1. mouse.

Organism-specific databases

CTD51703.
MGIMGI:1919129. Acsl5.

Phylogenomic databases

eggNOGCOG1022.
GeneTreeENSGT00690000101725.
HOGENOMHOG000159459.
HOVERGENHBG050452.
InParanoidQ8JZR0.
KOK01897.
OMAWTCADIG.
OrthoDBEOG71CFKN.
PhylomeDBQ8JZR0.
TreeFamTF313877.

Enzyme and pathway databases

BRENDA6.2.1.3. 3474.

Gene expression databases

ArrayExpressQ8JZR0.
BgeeQ8JZR0.
CleanExMM_ACSL5.
GenevestigatorQ8JZR0.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSACSL5. mouse.
NextBio408505.
PROQ8JZR0.
SOURCESearch...

Entry information

Entry nameACSL5_MOUSE
AccessionPrimary (citable) accession number: Q8JZR0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot