Reviewed, UniProtKB/Swiss-Prot Q8JZR0 (ACSL5_MOUSE)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase 5
    EC=6.2.1.3
Alternative name(s):
    Long-chain acyl-CoA synthetase 5
      Short name=LACS 5

Gene names

Name:	Acsl5
Synonyms:	Facl5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage By similarity. It was suggested that it may also stimulate fatty acid oxidation By similarity. At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL By similarity. May have a role in the survival of glioma cells By similarity. Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subcellular location	Mitochondrion By similarity. Endoplasmic reticulum By similarity. Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Signal-anchor Transmembrane
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from direct assay. Source: MGI mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 683	683	Long-chain-fatty-acid--CoA ligase 5		PRO_0000193113
Regions
	Transmembrane	12 – 32	21	Signal-anchor for type III membrane protein Potential
	Topological domain	33 – 683	651	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8JZR0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 734A1A5878741D70	FASTA	683	76,206
	10 20 30 40 50 60 MLFIFNFLFS PLPTPALICL LTFGTAIFLW LINRPQPVLP LIDLDNQSVG IEGGARRGAF 70 80 90 100 110 120 QKNNDLILYY FSDAKTLYEN FQRGLAVSDN GPCLGYRKPN QPYKWISYKQ VSDRAEYLGS 130 140 150 160 170 180 CLLHKGYKSS QDQFVGIFAQ NRPEWVISEL ACYTYSMVAV PLYDTLGTEA IIFVINRADI 190 200 210 220 230 240 PVVICDTPQK ATMLVENVEK GLTPGLKTII LMDPFDDDLM KRGEKCGVEM LSLHDAENIG 250 260 270 280 290 300 KENFKKPVPP KPEDLSVICF TSGTTGDPKG AMLTHENVVS NMAAFLKFLE PIFQPTSDDV 310 320 330 340 350 360 TISYLPLAHM FERLVQGILF SCGGKIGFFQ GDIRLLPDDM KALKPTVFPT VPRLLNRVYD 370 380 390 400 410 420 KVQNEAKTPL KKFLLNLAII SKFNEVKNGI IRRDSLWDKL VFSKIQGSLG GKVRLMITGA 430 440 450 460 470 480 APISTPVLTF FRAAMGCWVF EAYGQTECTG GCSITSPGDW TAGHVGTPVA CNFVKLEDVA 490 500 510 520 530 540 DMNYFSVNNE GEICIKGNNV FKGYLKDPEK TQEVLDKDGW LHTGDIGRWL PNGTLKIVDR 550 560 570 580 590 600 KKNIFKLAQG EYIAPEKIEN VYSRSRPVLQ VFVHGESLRS FLIGVVVPDP DSLPSFAAKI 610 620 630 640 650 660 GVKGSFEELC KNQCVKEAIL EDLQKIGKEG GLKSFEQVKS IFVHPEPFTI ENGLLTPTLK 670 680 AKRVELAKFF QTQIKSLYES IEE

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC031544 mRNA. Translation: AAH31544.1.
IPI	IPI00170363.
PIR	S49487.
RefSeq	NP_082252.1.
UniGene	Mm.292056
3D structure databases
SMR	Q8JZR0. Positions 75-590, 76-672.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8JZR0.
PTM databases
PhosphoSite	Q8JZR0.
Proteomic databases
PRIDE	Q8JZR0.
Genome annotation databases
Ensembl	ENSMUST00000043150; ENSMUSP00000046585; ENSMUSG00000024981; Mus musculus. [Genome view]
GeneID	433256.
KEGG	mmu:433256.
NMPDR	fig|10090.3.peg.5260.
UCSC	uc008hxp.1. mouse.
Organism-specific databases
CTD	433256.
MGI	MGI:1919129. Acsl5.
Phylogenomic databases
HOGENOM	HBG721674.
HOVERGEN	Q8JZR0.
InParanoid	Q8JZR0.
OMA	CWVFEAY.
OrthoDB	EOG97M4H3.
PhylomeDB	Q8JZR0.
Enzyme and pathway databases
BRENDA	6.2.1.3. 244.
Gene expression databases
ArrayExpress	Q8JZR0.
Bgee	Q8JZR0.
CleanEx	MM_ACSL5.
Genevestigator	Q8JZR0.
GermOnline	ENSMUSG00000024981. Mus musculus.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	408505.
SOURCE	Search...

Entry information

Entry name

ACSL5_MOUSE

Accession

Primary (citable) accession number: Q8JZR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)