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Protein

Kelch-like protein 2

Gene

Klhl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes growth of cell projections in oligodendrocyte precursors. Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4 (By similarity). Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation. Plays a role in the reorganization of the actin cytoskeleton.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 2
Gene namesi
Name:Klhl2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1924363. Klhl2.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Cell projectionruffle 1 Publication
  • Cell projection By similarity
  • Cell projectionlamellipodium By similarity
  • Cytoplasmcytosol 1 Publication

  • Note: A proportion colocalizes with the actin cytoskeleton (By similarity). When over-expressed, colocalizes with NPTXR in perinuclear aggresomes.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • Cul3-RING ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • lamellipodium Source: UniProtKB-SubCell
  • ruffle Source: UniProtKB-SubCell
  • stress fiber Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Kelch-like protein 2PRO_0000119102Add
BLAST

Proteomic databases

MaxQBiQ8JZP3.
PaxDbiQ8JZP3.
PRIDEiQ8JZP3.

PTM databases

PhosphoSiteiQ8JZP3.

Expressioni

Gene expression databases

BgeeiQ8JZP3.
CleanExiMM_KLHL2.
GenevisibleiQ8JZP3. MM.

Interactioni

Subunit structurei

Binds actin. Interacts with KLHL12. Interacts (via N-terminus) with FYN (via SH3 domain) (By similarity). Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. Interacts with NPTXR and CUL3.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218522. 3 interactions.
STRINGi10090.ENSMUSP00000034017.

Structurei

3D structure databases

ProteinModelPortaliQ8JZP3.
SMRiQ8JZP3. Positions 38-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 12368BTBPROSITE-ProRule annotationAdd
BLAST
Repeati308 – 35346Kelch 1Sequence analysisAdd
BLAST
Repeati354 – 40047Kelch 2Sequence analysisAdd
BLAST
Repeati402 – 44746Kelch 3Sequence analysisAdd
BLAST
Repeati449 – 49648Kelch 4Sequence analysisAdd
BLAST
Repeati497 – 54347Kelch 5Sequence analysisAdd
BLAST
Repeati545 – 59147Kelch 6Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Sequence analysis

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118931.
HOVERGENiHBG014286.
InParanoidiQ8JZP3.
KOiK10443.
OMAiANEWTYI.
OrthoDBiEOG7ZWD17.
PhylomeDBiQ8JZP3.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015916. Gal_Oxidase_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8JZP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METPPLPPAC TKQGHQKPLD SKDENPEKHC PLTVNPWHMK KAFKVMNELR
60 70 80 90 100
SQNLLCDVTI VAEDMEIPAH RVVLAACSPY FHAMFTGEMS ESRAKRVRIK
110 120 130 140 150
EVDGWTLRML VDYVYTAEIQ VTEENVQVLL PAAGLLQLQD VKKTCCEFLE
160 170 180 190 200
SQLHPVNCLG IRAFADMHAC TDLLNKANTY AEQHFADVVL SEEFLNLGIE
210 220 230 240 250
QVCSLISSDK LTISSEEKVF EAVIAWVNHD KDVRQEFMAR LMEHVRLPLL
260 270 280 290 300
PREYLVQRVE EEALVKNSSA CKDYLIEAMK YHLLPTEQRM LMKSVRTRLR
310 320 330 340 350
TPMNLPKLMV VVGGQAPKAI RSVECYDFKE ERWHQVAELP SRRCRAGMVY
360 370 380 390 400
MAGLVFAVGG FNGSLRVRTV DSYDPVKDQW TSVANMRDRR STLGAAVLNG
410 420 430 440 450
LLYAVGGFDG STGLSSVEAY NIKSNEWFHV APMNTRRSSV GVGVVGGLLY
460 470 480 490 500
AVGGYDGASR QCLSTVECYN ATANEWTYIA EMSTRRSGAG VGVLNNLLYA
510 520 530 540 550
VGGHDGPLVR KSVEVYDPTT NAWRQVADMN MCRRNAGVCA VNGLLYVVGG
560 570 580 590
DDGSCNLASV EYYNPTTDKW TVVSSCMSTG RSYAGVTVID KPL
Length:593
Mass (Da):65,983
Last modified:October 1, 2002 - v1
Checksum:i15B3ADEB5DDD0144
GO

Sequence cautioni

The sequence BAC27712.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024572 mRNA. Translation: AAH24572.1.
BC031142 mRNA. Translation: AAH31142.1.
BC031144 mRNA. Translation: AAH31144.1.
AK032116 mRNA. Translation: BAC27712.1. Different initiation.
RefSeqiNP_848748.2. NM_178633.3.
UniGeneiMm.350347.

Genome annotation databases

EnsembliENSMUST00000034017; ENSMUSP00000034017; ENSMUSG00000031605.
GeneIDi77113.
KEGGimmu:77113.
UCSCiuc057alr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024572 mRNA. Translation: AAH24572.1.
BC031142 mRNA. Translation: AAH31142.1.
BC031144 mRNA. Translation: AAH31144.1.
AK032116 mRNA. Translation: BAC27712.1. Different initiation.
RefSeqiNP_848748.2. NM_178633.3.
UniGeneiMm.350347.

3D structure databases

ProteinModelPortaliQ8JZP3.
SMRiQ8JZP3. Positions 38-591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218522. 3 interactions.
STRINGi10090.ENSMUSP00000034017.

PTM databases

PhosphoSiteiQ8JZP3.

Proteomic databases

MaxQBiQ8JZP3.
PaxDbiQ8JZP3.
PRIDEiQ8JZP3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034017; ENSMUSP00000034017; ENSMUSG00000031605.
GeneIDi77113.
KEGGimmu:77113.
UCSCiuc057alr.1. mouse.

Organism-specific databases

CTDi11275.
MGIiMGI:1924363. Klhl2.

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118931.
HOVERGENiHBG014286.
InParanoidiQ8JZP3.
KOiK10443.
OMAiANEWTYI.
OrthoDBiEOG7ZWD17.
PhylomeDBiQ8JZP3.
TreeFamiTF329218.

Miscellaneous databases

ChiTaRSiKlhl2. mouse.
PROiQ8JZP3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8JZP3.
CleanExiMM_KLHL2.
GenevisibleiQ8JZP3. MM.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015916. Gal_Oxidase_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech IIImported and FVB/NImported.
    Tissue: KidneyImported and LungImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 64-593.
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  3. "Interaction of an intracellular pentraxin with a BTB-Kelch protein is associated with ubiquitylation, aggregation and neuronal apoptosis."
    Tseng L.A., Bixby J.L.
    Mol. Cell. Neurosci. 47:254-264(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NPTXR AND CUL3.

Entry informationi

Entry nameiKLHL2_MOUSE
AccessioniPrimary (citable) accession number: Q8JZP3
Secondary accession number(s): Q8CCU0, Q8R3U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.