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Q8JZM0

- TFB1M_MOUSE

UniProt

Q8JZM0 - TFB1M_MOUSE

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Protein

Dimethyladenosine transferase 1, mitochondrial

Gene
Tfb1m
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei38 – 381S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei63 – 631S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei85 – 851S-adenosyl-L-methionine
Binding sitei111 – 1111S-adenosyl-L-methionine
Binding sitei141 – 1411S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiREACT_205251. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 1, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 1
Mitochondrial transcription factor B1
Short name:
mtTFB1
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
Gene namesi
Name:Tfb1m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:2146851. Tfb1m.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial nucleoid Source: Ensembl
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727Mitochondrion Reviewed predictionAdd
BLAST
Chaini28 – 345318Dimethyladenosine transferase 1, mitochondrialUniRule annotationPRO_0000273173Add
BLAST

Proteomic databases

MaxQBiQ8JZM0.
PaxDbiQ8JZM0.
PRIDEiQ8JZM0.

PTM databases

PhosphoSiteiQ8JZM0.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ8JZM0.
CleanExiMM_TFB1M.
GenevestigatoriQ8JZM0.

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035291.

Structurei

Secondary structure

1
345
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 3013
Beta strandi31 – 333
Helixi41 – 5111
Beta strandi58 – 625
Turni65 – 673
Helixi68 – 747
Turni75 – 773
Beta strandi79 – 846
Helixi88 – 903
Helixi91 – 10010
Turni102 – 1043
Beta strandi105 – 1084
Turni112 – 1143
Turni118 – 1203
Helixi123 – 1253
Beta strandi135 – 1406
Helixi144 – 16017
Helixi163 – 1675
Beta strandi171 – 1777
Helixi178 – 1858
Helixi195 – 2017
Beta strandi204 – 2129
Helixi214 – 2163
Beta strandi217 – 2193
Beta strandi225 – 2328
Helixi242 – 25312
Helixi260 – 2645
Helixi265 – 2673
Helixi270 – 2723
Helixi273 – 28412
Helixi292 – 2943
Helixi297 – 31317
Helixi317 – 3193
Helixi322 – 3265

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GC5X-ray1.80A1-345[»]
4GC9X-ray2.10A1-345[»]
ProteinModelPortaliQ8JZM0.
SMRiQ8JZM0. Positions 10-328.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 384S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0030.
GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227961.
HOVERGENiHBG082484.
InParanoidiQ8JZM0.
KOiK15266.
OMAiQKYCRRG.
OrthoDBiEOG7DJSMJ.
PhylomeDBiQ8JZM0.
TreeFamiTF300798.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JZM0-1 [UniParc]FASTAAdd to Basket

« Hide

MAASGKLGTF RLPPLPTIRE IIKLFGLRAV KQLSQNFLLD LRLTDKIVRK    50
AGSLADVYVY EVGPGPGGIT RSILNANVAE LLVVEKDTRF IPGLQMLSDA 100
APGKLRIVHG DVLTYKIEKA FPGNIRRQWE DDPPNVHIIG NLPFSVSTPL 150
IIKWLENISL KDGPFVYGRT KMTLTFQKEV AERLVATTGS KQHSRLSIMA 200
QYLCNVEHLF TIPGKAFVPK PKVDVGVVHL TPLIEPKIKQ PFKLVEKVVQ 250
NAFQFRRKYC HRGLGMLFPE AQRLESTGRL LQLADIDPTL RPTHLSLMHF 300
KSLCDVYRKM CDEDPQLFTY NFREELKQKK SKGQEKDGDP ESCGF 345
Length:345
Mass (Da):38,962
Last modified:October 1, 2002 - v1
Checksum:i97BF51EC95328AD5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF508971 mRNA. Translation: AAM33651.1.
BC032930 mRNA. Translation: AAH32930.1.
CCDSiCCDS28363.1.
RefSeqiNP_666186.1. NM_146074.1.
UniGeneiMm.226554.

Genome annotation databases

EnsembliENSMUST00000041003; ENSMUSP00000035291; ENSMUSG00000036983.
GeneIDi224481.
KEGGimmu:224481.
UCSCiuc008aes.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF508971 mRNA. Translation: AAM33651.1 .
BC032930 mRNA. Translation: AAH32930.1 .
CCDSi CCDS28363.1.
RefSeqi NP_666186.1. NM_146074.1.
UniGenei Mm.226554.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GC5 X-ray 1.80 A 1-345 [» ]
4GC9 X-ray 2.10 A 1-345 [» ]
ProteinModelPortali Q8JZM0.
SMRi Q8JZM0. Positions 10-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000035291.

PTM databases

PhosphoSitei Q8JZM0.

Proteomic databases

MaxQBi Q8JZM0.
PaxDbi Q8JZM0.
PRIDEi Q8JZM0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041003 ; ENSMUSP00000035291 ; ENSMUSG00000036983 .
GeneIDi 224481.
KEGGi mmu:224481.
UCSCi uc008aes.1. mouse.

Organism-specific databases

CTDi 51106.
MGIi MGI:2146851. Tfb1m.

Phylogenomic databases

eggNOGi COG0030.
GeneTreei ENSGT00530000063389.
HOGENOMi HOG000227961.
HOVERGENi HBG082484.
InParanoidi Q8JZM0.
KOi K15266.
OMAi QKYCRRG.
OrthoDBi EOG7DJSMJ.
PhylomeDBi Q8JZM0.
TreeFami TF300798.

Enzyme and pathway databases

Reactomei REACT_205251. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

NextBioi 377199.
PROi Q8JZM0.
SOURCEi Search...

Gene expression databases

Bgeei Q8JZM0.
CleanExi MM_TFB1M.
Genevestigatori Q8JZM0.

Family and domain databases

Gene3Di 1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPi MF_00607. 16SrRNA_methyltr_A.
InterProi IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR11727. PTHR11727. 1 hit.
Pfami PF00398. RrnaAD. 1 hit.
[Graphical view ]
SMARTi SM00650. rADc. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
    Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
    Nat. Genet. 31:289-294(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  3. "Characterization of the mouse genes for mitochondrial transcription factors B1 and B2."
    Rantanen A., Gaspari M., Falkenberg M., Gustafsson C.M., Larsson N.-G.
    Mamm. Genome 14:1-6(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Structural basis for S-adenosylmethionine binding and methyltransferase activity by mitochondrial transcription factor B1."
    Guja K.E., Venkataraman K., Yakubovskaya E., Shi H., Mejia E., Hambardjieva E., Karzai A.W., Garcia-Diaz M.
    Nucleic Acids Res. 41:7947-7959(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE.

Entry informationi

Entry nameiTFB1M_MOUSE
AccessioniPrimary (citable) accession number: Q8JZM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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