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Protein

Dimethyladenosine transferase 1, mitochondrial

Gene

Tfb1m

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei38S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei63S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionine1 Publication1
Binding sitei111S-adenosyl-L-methionine1 Publication1
Binding sitei141S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 1, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 1
Mitochondrial transcription factor B1
Short name:
mtTFB1
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
Gene namesi
Name:Tfb1m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146851. Tfb1m.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: GO_Central
  • mitochondrial nucleoid Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionSequence analysisAdd BLAST27
ChainiPRO_000027317328 – 345Dimethyladenosine transferase 1, mitochondrialAdd BLAST318

Proteomic databases

EPDiQ8JZM0.
MaxQBiQ8JZM0.
PaxDbiQ8JZM0.
PeptideAtlasiQ8JZM0.
PRIDEiQ8JZM0.

PTM databases

PhosphoSitePlusiQ8JZM0.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000036983.
CleanExiMM_TFB1M.
GenevisibleiQ8JZM0. MM.

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035291.

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 30Combined sources13
Beta strandi31 – 33Combined sources3
Helixi41 – 51Combined sources11
Beta strandi58 – 62Combined sources5
Turni65 – 67Combined sources3
Helixi68 – 74Combined sources7
Turni75 – 77Combined sources3
Beta strandi79 – 84Combined sources6
Helixi88 – 90Combined sources3
Helixi91 – 100Combined sources10
Turni102 – 104Combined sources3
Beta strandi105 – 108Combined sources4
Turni112 – 114Combined sources3
Turni118 – 120Combined sources3
Helixi123 – 125Combined sources3
Beta strandi135 – 140Combined sources6
Helixi144 – 160Combined sources17
Helixi163 – 167Combined sources5
Beta strandi171 – 177Combined sources7
Helixi178 – 185Combined sources8
Helixi195 – 201Combined sources7
Beta strandi204 – 212Combined sources9
Helixi214 – 216Combined sources3
Beta strandi217 – 219Combined sources3
Beta strandi225 – 232Combined sources8
Helixi242 – 253Combined sources12
Helixi260 – 264Combined sources5
Helixi265 – 267Combined sources3
Helixi270 – 272Combined sources3
Helixi273 – 284Combined sources12
Helixi292 – 294Combined sources3
Helixi297 – 313Combined sources17
Helixi317 – 319Combined sources3
Helixi322 – 326Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GC5X-ray1.80A1-345[»]
4GC9X-ray2.10A1-345[»]
ProteinModelPortaliQ8JZM0.
SMRiQ8JZM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38S-adenosyl-L-methionine binding4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0821. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227961.
HOVERGENiHBG082484.
InParanoidiQ8JZM0.
KOiK15266.
OMAiFQFRRKH.
OrthoDBiEOG091G0ALA.
PhylomeDBiQ8JZM0.
TreeFamiTF300798.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JZM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASGKLGTF RLPPLPTIRE IIKLFGLRAV KQLSQNFLLD LRLTDKIVRK
60 70 80 90 100
AGSLADVYVY EVGPGPGGIT RSILNANVAE LLVVEKDTRF IPGLQMLSDA
110 120 130 140 150
APGKLRIVHG DVLTYKIEKA FPGNIRRQWE DDPPNVHIIG NLPFSVSTPL
160 170 180 190 200
IIKWLENISL KDGPFVYGRT KMTLTFQKEV AERLVATTGS KQHSRLSIMA
210 220 230 240 250
QYLCNVEHLF TIPGKAFVPK PKVDVGVVHL TPLIEPKIKQ PFKLVEKVVQ
260 270 280 290 300
NAFQFRRKYC HRGLGMLFPE AQRLESTGRL LQLADIDPTL RPTHLSLMHF
310 320 330 340
KSLCDVYRKM CDEDPQLFTY NFREELKQKK SKGQEKDGDP ESCGF
Length:345
Mass (Da):38,962
Last modified:October 1, 2002 - v1
Checksum:i97BF51EC95328AD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF508971 mRNA. Translation: AAM33651.1.
BC032930 mRNA. Translation: AAH32930.1.
CCDSiCCDS28363.1.
RefSeqiNP_666186.1. NM_146074.1.
UniGeneiMm.226554.

Genome annotation databases

EnsembliENSMUST00000041003; ENSMUSP00000035291; ENSMUSG00000036983.
GeneIDi224481.
KEGGimmu:224481.
UCSCiuc008aes.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF508971 mRNA. Translation: AAM33651.1.
BC032930 mRNA. Translation: AAH32930.1.
CCDSiCCDS28363.1.
RefSeqiNP_666186.1. NM_146074.1.
UniGeneiMm.226554.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GC5X-ray1.80A1-345[»]
4GC9X-ray2.10A1-345[»]
ProteinModelPortaliQ8JZM0.
SMRiQ8JZM0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035291.

PTM databases

PhosphoSitePlusiQ8JZM0.

Proteomic databases

EPDiQ8JZM0.
MaxQBiQ8JZM0.
PaxDbiQ8JZM0.
PeptideAtlasiQ8JZM0.
PRIDEiQ8JZM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041003; ENSMUSP00000035291; ENSMUSG00000036983.
GeneIDi224481.
KEGGimmu:224481.
UCSCiuc008aes.1. mouse.

Organism-specific databases

CTDi51106.
MGIiMGI:2146851. Tfb1m.

Phylogenomic databases

eggNOGiKOG0821. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227961.
HOVERGENiHBG082484.
InParanoidiQ8JZM0.
KOiK15266.
OMAiFQFRRKH.
OrthoDBiEOG091G0ALA.
PhylomeDBiQ8JZM0.
TreeFamiTF300798.

Enzyme and pathway databases

ReactomeiR-MMU-2151201. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

PROiQ8JZM0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036983.
CleanExiMM_TFB1M.
GenevisibleiQ8JZM0. MM.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFB1M_MOUSE
AccessioniPrimary (citable) accession number: Q8JZM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.