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Protein

Dimethyladenosine transferase 1, mitochondrial

Gene

Tfb1m

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei38S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei63S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionine1 Publication1
Binding sitei111S-adenosyl-L-methionine1 Publication1
Binding sitei141S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, RNA-binding, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 1, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 1
Mitochondrial transcription factor B1
Short name:
mtTFB1
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
Gene namesi
Name:Tfb1m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146851 Tfb1m

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionSequence analysisAdd BLAST27
ChainiPRO_000027317328 – 345Dimethyladenosine transferase 1, mitochondrialAdd BLAST318

Proteomic databases

EPDiQ8JZM0
MaxQBiQ8JZM0
PaxDbiQ8JZM0
PeptideAtlasiQ8JZM0
PRIDEiQ8JZM0

PTM databases

PhosphoSitePlusiQ8JZM0

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000036983
CleanExiMM_TFB1M
GenevisibleiQ8JZM0 MM

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035291

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 30Combined sources13
Beta strandi31 – 33Combined sources3
Helixi41 – 51Combined sources11
Beta strandi58 – 62Combined sources5
Turni65 – 67Combined sources3
Helixi68 – 74Combined sources7
Turni75 – 77Combined sources3
Beta strandi79 – 84Combined sources6
Helixi88 – 90Combined sources3
Helixi91 – 100Combined sources10
Turni102 – 104Combined sources3
Beta strandi105 – 108Combined sources4
Turni112 – 114Combined sources3
Turni118 – 120Combined sources3
Helixi123 – 125Combined sources3
Beta strandi135 – 140Combined sources6
Helixi144 – 160Combined sources17
Helixi163 – 167Combined sources5
Beta strandi171 – 177Combined sources7
Helixi178 – 185Combined sources8
Helixi195 – 201Combined sources7
Beta strandi204 – 212Combined sources9
Helixi214 – 216Combined sources3
Beta strandi217 – 219Combined sources3
Beta strandi225 – 232Combined sources8
Helixi242 – 253Combined sources12
Helixi260 – 264Combined sources5
Helixi265 – 267Combined sources3
Helixi270 – 272Combined sources3
Helixi273 – 284Combined sources12
Helixi292 – 294Combined sources3
Helixi297 – 313Combined sources17
Helixi317 – 319Combined sources3
Helixi322 – 326Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GC5X-ray1.80A1-345[»]
4GC9X-ray2.10A1-345[»]
ProteinModelPortaliQ8JZM0
SMRiQ8JZM0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38S-adenosyl-L-methionine binding4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0821 Eukaryota
COG0030 LUCA
GeneTreeiENSGT00530000063389
HOGENOMiHOG000227961
HOVERGENiHBG082484
InParanoidiQ8JZM0
KOiK15266
OMAiSVMSQIW
OrthoDBiEOG091G0ALA
PhylomeDBiQ8JZM0
TreeFamiTF300798

Family and domain databases

Gene3Di1.10.8.100, 1 hit
HAMAPiMF_00607 16SrRNA_methyltr_A, 1 hit
InterProiView protein in InterPro
IPR001737 KsgA/Erm
IPR023165 rRNA_Ade_diMease-like
IPR020596 rRNA_Ade_Mease_Trfase_CS
IPR020598 rRNA_Ade_methylase_Trfase_N
IPR011530 rRNA_adenine_dimethylase
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11727 PTHR11727, 1 hit
PfamiView protein in Pfam
PF00398 RrnaAD, 1 hit
SMARTiView protein in SMART
SM00650 rADc, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00755 ksgA, 1 hit
PROSITEiView protein in PROSITE
PS01131 RRNA_A_DIMETH, 1 hit
PS51689 SAM_RNA_A_N6_MT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JZM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASGKLGTF RLPPLPTIRE IIKLFGLRAV KQLSQNFLLD LRLTDKIVRK
60 70 80 90 100
AGSLADVYVY EVGPGPGGIT RSILNANVAE LLVVEKDTRF IPGLQMLSDA
110 120 130 140 150
APGKLRIVHG DVLTYKIEKA FPGNIRRQWE DDPPNVHIIG NLPFSVSTPL
160 170 180 190 200
IIKWLENISL KDGPFVYGRT KMTLTFQKEV AERLVATTGS KQHSRLSIMA
210 220 230 240 250
QYLCNVEHLF TIPGKAFVPK PKVDVGVVHL TPLIEPKIKQ PFKLVEKVVQ
260 270 280 290 300
NAFQFRRKYC HRGLGMLFPE AQRLESTGRL LQLADIDPTL RPTHLSLMHF
310 320 330 340
KSLCDVYRKM CDEDPQLFTY NFREELKQKK SKGQEKDGDP ESCGF
Length:345
Mass (Da):38,962
Last modified:October 1, 2002 - v1
Checksum:i97BF51EC95328AD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF508971 mRNA Translation: AAM33651.1
BC032930 mRNA Translation: AAH32930.1
CCDSiCCDS28363.1
RefSeqiNP_666186.1, NM_146074.1
UniGeneiMm.226554

Genome annotation databases

EnsembliENSMUST00000041003; ENSMUSP00000035291; ENSMUSG00000036983
GeneIDi224481
KEGGimmu:224481
UCSCiuc008aes.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTFB1M_MOUSE
AccessioniPrimary (citable) accession number: Q8JZM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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