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Q8JZM0 (TFB1M_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dimethyladenosine transferase 1, mitochondrial

EC=2.1.1.-
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 1
Mitochondrial transcription factor B1
Short name=mtTFB1
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
Gene names
Name:Tfb1m
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity By similarity. HAMAP-Rule MF_00607

Subunit structure

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM By similarity.

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_00607.

Tissue specificity

Ubiquitously expressed. Ref.3

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 345318Dimethyladenosine transferase 1, mitochondrial HAMAP-Rule MF_00607
PRO_0000273173

Regions

Region35 – 384S-adenosyl-L-methionine binding HAMAP-Rule MF_00607

Sites

Binding site361S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site381S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site631S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site851S-adenosyl-L-methionine
Binding site1111S-adenosyl-L-methionine
Binding site1411S-adenosyl-L-methionine By similarity

Secondary structure

............................................................ 345
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8JZM0 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 97BF51EC95328AD5

FASTA34538,962
        10         20         30         40         50         60 
MAASGKLGTF RLPPLPTIRE IIKLFGLRAV KQLSQNFLLD LRLTDKIVRK AGSLADVYVY 

        70         80         90        100        110        120 
EVGPGPGGIT RSILNANVAE LLVVEKDTRF IPGLQMLSDA APGKLRIVHG DVLTYKIEKA 

       130        140        150        160        170        180 
FPGNIRRQWE DDPPNVHIIG NLPFSVSTPL IIKWLENISL KDGPFVYGRT KMTLTFQKEV 

       190        200        210        220        230        240 
AERLVATTGS KQHSRLSIMA QYLCNVEHLF TIPGKAFVPK PKVDVGVVHL TPLIEPKIKQ 

       250        260        270        280        290        300 
PFKLVEKVVQ NAFQFRRKYC HRGLGMLFPE AQRLESTGRL LQLADIDPTL RPTHLSLMHF 

       310        320        330        340 
KSLCDVYRKM CDEDPQLFTY NFREELKQKK SKGQEKDGDP ESCGF 

« Hide

References

« Hide 'large scale' references
[1]"Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
Nat. Genet. 31:289-294(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[3]"Characterization of the mouse genes for mitochondrial transcription factors B1 and B2."
Rantanen A., Gaspari M., Falkenberg M., Gustafsson C.M., Larsson N.-G.
Mamm. Genome 14:1-6(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Structural basis for S-adenosylmethionine binding and methyltransferase activity by mitochondrial transcription factor B1."
Guja K.E., Venkataraman K., Yakubovskaya E., Shi H., Mejia E., Hambardjieva E., Karzai A.W., Garcia-Diaz M.
Nucleic Acids Res. 41:7947-7959(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF508971 mRNA. Translation: AAM33651.1.
BC032930 mRNA. Translation: AAH32930.1.
CCDSCCDS28363.1.
RefSeqNP_666186.1. NM_146074.1.
UniGeneMm.226554.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GC5X-ray1.80A1-345[»]
4GC9X-ray2.10A1-345[»]
ProteinModelPortalQ8JZM0.
SMRQ8JZM0. Positions 10-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000035291.

PTM databases

PhosphoSiteQ8JZM0.

Proteomic databases

MaxQBQ8JZM0.
PaxDbQ8JZM0.
PRIDEQ8JZM0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000041003; ENSMUSP00000035291; ENSMUSG00000036983.
GeneID224481.
KEGGmmu:224481.
UCSCuc008aes.1. mouse.

Organism-specific databases

CTD51106.
MGIMGI:2146851. Tfb1m.

Phylogenomic databases

eggNOGCOG0030.
GeneTreeENSGT00530000063389.
HOGENOMHOG000227961.
HOVERGENHBG082484.
InParanoidQ8JZM0.
KOK15266.
OMAQKYCRRG.
OrthoDBEOG7DJSMJ.
PhylomeDBQ8JZM0.
TreeFamTF300798.

Gene expression databases

BgeeQ8JZM0.
CleanExMM_TFB1M.
GenevestigatorQ8JZM0.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio377199.
PROQ8JZM0.
SOURCESearch...

Entry information

Entry nameTFB1M_MOUSE
AccessionPrimary (citable) accession number: Q8JZM0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot