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Q8JZM0

- TFB1M_MOUSE

UniProt

Q8JZM0 - TFB1M_MOUSE

Protein

Dimethyladenosine transferase 1, mitochondrial

Gene

Tfb1m

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei38 – 381S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei63 – 631S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei85 – 851S-adenosyl-L-methionine1 Publication
    Binding sitei111 – 1111S-adenosyl-L-methionine1 Publication
    Binding sitei141 – 1411S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    ReactomeiREACT_205251. Transcriptional activation of mitochondrial biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dimethyladenosine transferase 1, mitochondrial (EC:2.1.1.-)
    Alternative name(s):
    Mitochondrial 12S rRNA dimethylase 1
    Mitochondrial transcription factor B1
    Short name:
    mtTFB1
    S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
    Gene namesi
    Name:Tfb1m
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2146851. Tfb1m.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial nucleoid Source: Ensembl
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
    BLAST
    Chaini28 – 345318Dimethyladenosine transferase 1, mitochondrialPRO_0000273173Add
    BLAST

    Proteomic databases

    MaxQBiQ8JZM0.
    PaxDbiQ8JZM0.
    PRIDEiQ8JZM0.

    PTM databases

    PhosphoSiteiQ8JZM0.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    BgeeiQ8JZM0.
    CleanExiMM_TFB1M.
    GenevestigatoriQ8JZM0.

    Interactioni

    Subunit structurei

    Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000035291.

    Structurei

    Secondary structure

    1
    345
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi18 – 3013
    Beta strandi31 – 333
    Helixi41 – 5111
    Beta strandi58 – 625
    Turni65 – 673
    Helixi68 – 747
    Turni75 – 773
    Beta strandi79 – 846
    Helixi88 – 903
    Helixi91 – 10010
    Turni102 – 1043
    Beta strandi105 – 1084
    Turni112 – 1143
    Turni118 – 1203
    Helixi123 – 1253
    Beta strandi135 – 1406
    Helixi144 – 16017
    Helixi163 – 1675
    Beta strandi171 – 1777
    Helixi178 – 1858
    Helixi195 – 2017
    Beta strandi204 – 2129
    Helixi214 – 2163
    Beta strandi217 – 2193
    Beta strandi225 – 2328
    Helixi242 – 25312
    Helixi260 – 2645
    Helixi265 – 2673
    Helixi270 – 2723
    Helixi273 – 28412
    Helixi292 – 2943
    Helixi297 – 31317
    Helixi317 – 3193
    Helixi322 – 3265

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GC5X-ray1.80A1-345[»]
    4GC9X-ray2.10A1-345[»]
    ProteinModelPortaliQ8JZM0.
    SMRiQ8JZM0. Positions 10-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni35 – 384S-adenosyl-L-methionine binding

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0030.
    GeneTreeiENSGT00530000063389.
    HOGENOMiHOG000227961.
    HOVERGENiHBG082484.
    InParanoidiQ8JZM0.
    KOiK15266.
    OMAiQKYCRRG.
    OrthoDBiEOG7DJSMJ.
    PhylomeDBiQ8JZM0.
    TreeFamiTF300798.

    Family and domain databases

    Gene3Di1.10.8.100. 1 hit.
    3.40.50.150. 1 hit.
    HAMAPiMF_00607. 16SrRNA_methyltr_A.
    InterProiIPR023165. rRNA_Ade_diMease-like.
    IPR020596. rRNA_Ade_Mease_Trfase_CS.
    IPR001737. rRNA_Ade_methylase_transferase.
    IPR020598. rRNA_Ade_methylase_Trfase_N.
    IPR011530. rRNA_adenine_dimethylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR11727. PTHR11727. 1 hit.
    PfamiPF00398. RrnaAD. 1 hit.
    [Graphical view]
    SMARTiSM00650. rADc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
    PS51689. SAM_RNA_A_N6_MT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8JZM0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASGKLGTF RLPPLPTIRE IIKLFGLRAV KQLSQNFLLD LRLTDKIVRK    50
    AGSLADVYVY EVGPGPGGIT RSILNANVAE LLVVEKDTRF IPGLQMLSDA 100
    APGKLRIVHG DVLTYKIEKA FPGNIRRQWE DDPPNVHIIG NLPFSVSTPL 150
    IIKWLENISL KDGPFVYGRT KMTLTFQKEV AERLVATTGS KQHSRLSIMA 200
    QYLCNVEHLF TIPGKAFVPK PKVDVGVVHL TPLIEPKIKQ PFKLVEKVVQ 250
    NAFQFRRKYC HRGLGMLFPE AQRLESTGRL LQLADIDPTL RPTHLSLMHF 300
    KSLCDVYRKM CDEDPQLFTY NFREELKQKK SKGQEKDGDP ESCGF 345
    Length:345
    Mass (Da):38,962
    Last modified:October 1, 2002 - v1
    Checksum:i97BF51EC95328AD5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF508971 mRNA. Translation: AAM33651.1.
    BC032930 mRNA. Translation: AAH32930.1.
    CCDSiCCDS28363.1.
    RefSeqiNP_666186.1. NM_146074.1.
    UniGeneiMm.226554.

    Genome annotation databases

    EnsembliENSMUST00000041003; ENSMUSP00000035291; ENSMUSG00000036983.
    GeneIDi224481.
    KEGGimmu:224481.
    UCSCiuc008aes.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF508971 mRNA. Translation: AAM33651.1 .
    BC032930 mRNA. Translation: AAH32930.1 .
    CCDSi CCDS28363.1.
    RefSeqi NP_666186.1. NM_146074.1.
    UniGenei Mm.226554.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GC5 X-ray 1.80 A 1-345 [» ]
    4GC9 X-ray 2.10 A 1-345 [» ]
    ProteinModelPortali Q8JZM0.
    SMRi Q8JZM0. Positions 10-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000035291.

    PTM databases

    PhosphoSitei Q8JZM0.

    Proteomic databases

    MaxQBi Q8JZM0.
    PaxDbi Q8JZM0.
    PRIDEi Q8JZM0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041003 ; ENSMUSP00000035291 ; ENSMUSG00000036983 .
    GeneIDi 224481.
    KEGGi mmu:224481.
    UCSCi uc008aes.1. mouse.

    Organism-specific databases

    CTDi 51106.
    MGIi MGI:2146851. Tfb1m.

    Phylogenomic databases

    eggNOGi COG0030.
    GeneTreei ENSGT00530000063389.
    HOGENOMi HOG000227961.
    HOVERGENi HBG082484.
    InParanoidi Q8JZM0.
    KOi K15266.
    OMAi QKYCRRG.
    OrthoDBi EOG7DJSMJ.
    PhylomeDBi Q8JZM0.
    TreeFami TF300798.

    Enzyme and pathway databases

    Reactomei REACT_205251. Transcriptional activation of mitochondrial biogenesis.

    Miscellaneous databases

    NextBioi 377199.
    PROi Q8JZM0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8JZM0.
    CleanExi MM_TFB1M.
    Genevestigatori Q8JZM0.

    Family and domain databases

    Gene3Di 1.10.8.100. 1 hit.
    3.40.50.150. 1 hit.
    HAMAPi MF_00607. 16SrRNA_methyltr_A.
    InterProi IPR023165. rRNA_Ade_diMease-like.
    IPR020596. rRNA_Ade_Mease_Trfase_CS.
    IPR001737. rRNA_Ade_methylase_transferase.
    IPR020598. rRNA_Ade_methylase_Trfase_N.
    IPR011530. rRNA_adenine_dimethylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR11727. PTHR11727. 1 hit.
    Pfami PF00398. RrnaAD. 1 hit.
    [Graphical view ]
    SMARTi SM00650. rADc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS01131. RRNA_A_DIMETH. 1 hit.
    PS51689. SAM_RNA_A_N6_MT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
      Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
      Nat. Genet. 31:289-294(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    3. "Characterization of the mouse genes for mitochondrial transcription factors B1 and B2."
      Rantanen A., Gaspari M., Falkenberg M., Gustafsson C.M., Larsson N.-G.
      Mamm. Genome 14:1-6(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Structural basis for S-adenosylmethionine binding and methyltransferase activity by mitochondrial transcription factor B1."
      Guja K.E., Venkataraman K., Yakubovskaya E., Shi H., Mejia E., Hambardjieva E., Karzai A.W., Garcia-Diaz M.
      Nucleic Acids Res. 41:7947-7959(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE.

    Entry informationi

    Entry nameiTFB1M_MOUSE
    AccessioniPrimary (citable) accession number: Q8JZM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3