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Protein

Thiamine-triphosphatase

Gene

Thtpa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolase highly specific for thiamine triphosphate (ThTP).1 Publication

Catalytic activityi

Thiamine triphosphate + H2O = thiamine diphosphate + phosphate.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

pH dependencei

Optimum pH is 7.5-9.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium1
Metal bindingi9Magnesium1
Binding sitei11Substrate1
Binding sitei55Substrate1
Binding sitei57Substrate1
Binding sitei65Substrate1
Binding sitei125Substrate1
Metal bindingi145Magnesium1
Metal bindingi157Magnesium1
Binding sitei157SubstrateBy similarity1
Metal bindingi159Magnesium1
Binding sitei193Substrate1

GO - Molecular functioni

  • magnesium ion binding Source: UniProtKB
  • thiamin-triphosphatase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.6.1.28. 3474.
ReactomeiR-MMU-196819. Vitamin B1 (thiamin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine-triphosphatase (EC:3.6.1.28)
Short name:
ThTPase
Gene namesi
Name:Thtpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2446078. Thtpa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002214922 – 224Thiamine-triphosphataseAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8JZL3.
PaxDbiQ8JZL3.
PeptideAtlasiQ8JZL3.
PRIDEiQ8JZL3.

PTM databases

PhosphoSitePlusiQ8JZL3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045691.
CleanExiMM_THTPA.
ExpressionAtlasiQ8JZL3. baseline and differential.
GenevisibleiQ8JZL3. MM.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiQ8JZL3. 1 interactor.
MINTiMINT-4137740.
STRINGi10090.ENSMUSP00000056026.

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi18 – 24Combined sources7
Beta strandi28 – 41Combined sources14
Helixi46 – 49Combined sources4
Beta strandi53 – 57Combined sources5
Turni58 – 60Combined sources3
Beta strandi61 – 67Combined sources7
Beta strandi79 – 82Combined sources4
Helixi85 – 96Combined sources12
Helixi106 – 109Combined sources4
Helixi111 – 113Combined sources3
Beta strandi116 – 130Combined sources15
Beta strandi142 – 149Combined sources8
Turni150 – 152Combined sources3
Beta strandi153 – 164Combined sources12
Helixi165 – 167Combined sources3
Helixi168 – 182Combined sources15
Beta strandi183 – 185Combined sources3
Helixi193 – 201Combined sources9
Helixi203 – 213Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JMUNMR-A2-224[»]
5A64X-ray2.10A/B1-224[»]
5A65X-ray1.98A/B3-215[»]
ProteinModelPortaliQ8JZL3.
SMRiQ8JZL3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8JZL3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 201CYTHPROSITE-ProRule annotationAdd BLAST197

Sequence similaritiesi

Belongs to the ThTPase family.Curated
Contains 1 CYTH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J0T6. Eukaryota.
ENOG4111XGF. LUCA.
GeneTreeiENSGT00390000005996.
HOGENOMiHOG000154571.
HOVERGENiHBG057173.
InParanoidiQ8JZL3.
KOiK05307.
OMAiRDTYYDT.
OrthoDBiEOG091G11DC.
PhylomeDBiQ8JZL3.
TreeFamiTF333398.

Family and domain databases

Gene3Di2.40.320.10. 1 hit.
InterProiIPR033469. CYTH-like_dom.
IPR023577. CYTH_domain.
IPR012177. ThTPase.
[Graphical view]
PfamiPF01928. CYTH. 1 hit.
[Graphical view]
PIRSFiPIRSF036561. ThTPase. 1 hit.
SMARTiSM01118. CYTH. 1 hit.
[Graphical view]
SUPFAMiSSF55154. SSF55154. 1 hit.
PROSITEiPS51707. CYTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JZL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQGLIEVER KFAPGPDTEE RLQELGATLE HRVTFRDTYY DTSELSLMLS
60 70 80 90 100
DHWLRQREGS GWELKCPGVT GVSGPHNEYV EVTSEAAIVA QLFELLGSGE
110 120 130 140 150
QKPAGVAAVL GSLKLQEVAS FITTRSSWKL ALSGAHGQEP QLTIDLDSAD
160 170 180 190 200
FGYAVGEVEA MVHEKAEVPA ALEKIITVSS MLGVPAQEEA PAKLMVYLQR
210 220
FRPLDYQRLL EAASSGEATG DSAS
Length:224
Mass (Da):24,264
Last modified:January 23, 2007 - v3
Checksum:iD33C844FDA8277D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF432864 mRNA. Translation: AAM22405.1.
AK132479 mRNA. Translation: BAE21189.1.
BC025562 mRNA. Translation: AAH25562.1.
CCDSiCCDS27108.1.
RefSeqiNP_694723.1. NM_153083.5.
UniGeneiMm.319204.

Genome annotation databases

EnsembliENSMUST00000050575; ENSMUSP00000056026; ENSMUSG00000045691.
GeneIDi105663.
KEGGimmu:105663.
UCSCiuc007tyb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF432864 mRNA. Translation: AAM22405.1.
AK132479 mRNA. Translation: BAE21189.1.
BC025562 mRNA. Translation: AAH25562.1.
CCDSiCCDS27108.1.
RefSeqiNP_694723.1. NM_153083.5.
UniGeneiMm.319204.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JMUNMR-A2-224[»]
5A64X-ray2.10A/B1-224[»]
5A65X-ray1.98A/B3-215[»]
ProteinModelPortaliQ8JZL3.
SMRiQ8JZL3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8JZL3. 1 interactor.
MINTiMINT-4137740.
STRINGi10090.ENSMUSP00000056026.

PTM databases

PhosphoSitePlusiQ8JZL3.

Proteomic databases

MaxQBiQ8JZL3.
PaxDbiQ8JZL3.
PeptideAtlasiQ8JZL3.
PRIDEiQ8JZL3.

Protocols and materials databases

DNASUi105663.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050575; ENSMUSP00000056026; ENSMUSG00000045691.
GeneIDi105663.
KEGGimmu:105663.
UCSCiuc007tyb.2. mouse.

Organism-specific databases

CTDi79178.
MGIiMGI:2446078. Thtpa.

Phylogenomic databases

eggNOGiENOG410J0T6. Eukaryota.
ENOG4111XGF. LUCA.
GeneTreeiENSGT00390000005996.
HOGENOMiHOG000154571.
HOVERGENiHBG057173.
InParanoidiQ8JZL3.
KOiK05307.
OMAiRDTYYDT.
OrthoDBiEOG091G11DC.
PhylomeDBiQ8JZL3.
TreeFamiTF333398.

Enzyme and pathway databases

BRENDAi3.6.1.28. 3474.
ReactomeiR-MMU-196819. Vitamin B1 (thiamin) metabolism.

Miscellaneous databases

EvolutionaryTraceiQ8JZL3.
PROiQ8JZL3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045691.
CleanExiMM_THTPA.
ExpressionAtlasiQ8JZL3. baseline and differential.
GenevisibleiQ8JZL3. MM.

Family and domain databases

Gene3Di2.40.320.10. 1 hit.
InterProiIPR033469. CYTH-like_dom.
IPR023577. CYTH_domain.
IPR012177. ThTPase.
[Graphical view]
PfamiPF01928. CYTH. 1 hit.
[Graphical view]
PIRSFiPIRSF036561. ThTPase. 1 hit.
SMARTiSM01118. CYTH. 1 hit.
[Graphical view]
SUPFAMiSSF55154. SSF55154. 1 hit.
PROSITEiPS51707. CYTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHTPA_MOUSE
AccessioniPrimary (citable) accession number: Q8JZL3
Secondary accession number(s): Q3V1G2, Q8C3P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.