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Q8JZL3

- THTPA_MOUSE

UniProt

Q8JZL3 - THTPA_MOUSE

Protein

Thiamine-triphosphatase

Gene

Thtpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Hydrolase highly specific for thiamine triphosphate (ThTP).1 Publication

    Catalytic activityi

    Thiamine triphosphate + H2O = thiamine diphosphate + phosphate.1 Publication

    Cofactori

    Binds 1 Mg2+ ion per subunit.1 Publication

    pH dependencei

    Optimum pH is 7.5-9.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71Magnesium
    Metal bindingi9 – 91Magnesium
    Binding sitei11 – 111Substrate
    Binding sitei55 – 551Substrate
    Binding sitei57 – 571Substrate
    Binding sitei65 – 651Substrate
    Binding sitei125 – 1251Substrate
    Metal bindingi145 – 1451Magnesium
    Metal bindingi157 – 1571Magnesium
    Binding sitei157 – 1571SubstrateBy similarity
    Metal bindingi159 – 1591Magnesium
    Binding sitei193 – 1931Substrate

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB
    2. thiamin-triphosphatase activity Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: Ensembl
    2. thiamine diphosphate metabolic process Source: UniProtKB
    3. thiamine metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_223427. Vitamin B1 (thiamin) metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine-triphosphatase (EC:3.6.1.28)
    Short name:
    ThTPase
    Gene namesi
    Name:Thtpa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2446078. Thtpa.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 224223Thiamine-triphosphatasePRO_0000221492Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8JZL3.
    PaxDbiQ8JZL3.
    PRIDEiQ8JZL3.

    PTM databases

    PhosphoSiteiQ8JZL3.

    Expressioni

    Gene expression databases

    BgeeiQ8JZL3.
    CleanExiMM_THTPA.
    GenevestigatoriQ8JZL3.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    IntActiQ8JZL3. 1 interaction.
    MINTiMINT-4137740.

    Structurei

    Secondary structure

    1
    224
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 1412
    Helixi18 – 258
    Beta strandi28 – 4114
    Helixi46 – 494
    Beta strandi53 – 575
    Turni58 – 603
    Beta strandi61 – 666
    Beta strandi80 – 823
    Helixi85 – 9612
    Helixi106 – 1138
    Beta strandi116 – 13116
    Beta strandi141 – 1499
    Turni150 – 1523
    Beta strandi153 – 16311
    Helixi165 – 1673
    Helixi168 – 18215
    Beta strandi183 – 1853
    Helixi193 – 2019
    Helixi203 – 21210

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JMUNMR-A2-224[»]
    ProteinModelPortaliQ8JZL3.
    SMRiQ8JZL3. Positions 2-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8JZL3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 201197CYTHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThTPase family.Curated
    Contains 1 CYTH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG42323.
    GeneTreeiENSGT00390000005996.
    HOGENOMiHOG000154571.
    HOVERGENiHBG057173.
    InParanoidiQ8JZL3.
    KOiK05307.
    OMAiRDTYYDT.
    OrthoDBiEOG7288SN.
    PhylomeDBiQ8JZL3.
    TreeFamiTF333398.

    Family and domain databases

    Gene3Di2.40.320.10. 1 hit.
    InterProiIPR023577. CYTH-like_domain.
    IPR012177. ThTPase.
    [Graphical view]
    PANTHERiPTHR14586. PTHR14586. 1 hit.
    PfamiPF01928. CYTH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036561. ThTPase. 1 hit.
    SUPFAMiSSF55154. SSF55154. 1 hit.
    PROSITEiPS51707. CYTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8JZL3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQGLIEVER KFAPGPDTEE RLQELGATLE HRVTFRDTYY DTSELSLMLS    50
    DHWLRQREGS GWELKCPGVT GVSGPHNEYV EVTSEAAIVA QLFELLGSGE 100
    QKPAGVAAVL GSLKLQEVAS FITTRSSWKL ALSGAHGQEP QLTIDLDSAD 150
    FGYAVGEVEA MVHEKAEVPA ALEKIITVSS MLGVPAQEEA PAKLMVYLQR 200
    FRPLDYQRLL EAASSGEATG DSAS 224
    Length:224
    Mass (Da):24,264
    Last modified:January 23, 2007 - v3
    Checksum:iD33C844FDA8277D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF432864 mRNA. Translation: AAM22405.1.
    AK132479 mRNA. Translation: BAE21189.1.
    BC025562 mRNA. Translation: AAH25562.1.
    CCDSiCCDS27108.1.
    RefSeqiNP_694723.1. NM_153083.5.
    UniGeneiMm.319204.

    Genome annotation databases

    EnsembliENSMUST00000050575; ENSMUSP00000056026; ENSMUSG00000045691.
    GeneIDi105663.
    KEGGimmu:105663.
    UCSCiuc007tyb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF432864 mRNA. Translation: AAM22405.1 .
    AK132479 mRNA. Translation: BAE21189.1 .
    BC025562 mRNA. Translation: AAH25562.1 .
    CCDSi CCDS27108.1.
    RefSeqi NP_694723.1. NM_153083.5.
    UniGenei Mm.319204.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JMU NMR - A 2-224 [» ]
    ProteinModelPortali Q8JZL3.
    SMRi Q8JZL3. Positions 2-224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8JZL3. 1 interaction.
    MINTi MINT-4137740.

    PTM databases

    PhosphoSitei Q8JZL3.

    Proteomic databases

    MaxQBi Q8JZL3.
    PaxDbi Q8JZL3.
    PRIDEi Q8JZL3.

    Protocols and materials databases

    DNASUi 105663.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050575 ; ENSMUSP00000056026 ; ENSMUSG00000045691 .
    GeneIDi 105663.
    KEGGi mmu:105663.
    UCSCi uc007tyb.2. mouse.

    Organism-specific databases

    CTDi 79178.
    MGIi MGI:2446078. Thtpa.

    Phylogenomic databases

    eggNOGi NOG42323.
    GeneTreei ENSGT00390000005996.
    HOGENOMi HOG000154571.
    HOVERGENi HBG057173.
    InParanoidi Q8JZL3.
    KOi K05307.
    OMAi RDTYYDT.
    OrthoDBi EOG7288SN.
    PhylomeDBi Q8JZL3.
    TreeFami TF333398.

    Enzyme and pathway databases

    Reactomei REACT_223427. Vitamin B1 (thiamin) metabolism.

    Miscellaneous databases

    EvolutionaryTracei Q8JZL3.
    NextBioi 357818.
    PROi Q8JZL3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8JZL3.
    CleanExi MM_THTPA.
    Genevestigatori Q8JZL3.

    Family and domain databases

    Gene3Di 2.40.320.10. 1 hit.
    InterProi IPR023577. CYTH-like_domain.
    IPR012177. ThTPase.
    [Graphical view ]
    PANTHERi PTHR14586. PTHR14586. 1 hit.
    Pfami PF01928. CYTH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036561. ThTPase. 1 hit.
    SUPFAMi SSF55154. SSF55154. 1 hit.
    PROSITEi PS51707. CYTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of mouse thiamine triphosphatase cDNA."
      Lakaye B., Coumans B., Makarchikov A., Grisar T., Bettendorff L.
      Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Skin.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "Structural basis for the catalytic mechanism of mammalian 25-kDa thiamine triphosphatase."
      Song J., Bettendorff L., Tonelli M., Markley J.L.
      J. Biol. Chem. 283:10939-10948(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 2-223, SUBUNIT, MAGNESIUM-BINDING SITES, SUBSTRATE-BINDING SITES, CATALYTIC ACTIVITY, FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiTHTPA_MOUSE
    AccessioniPrimary (citable) accession number: Q8JZL3
    Secondary accession number(s): Q3V1G2, Q8C3P9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3