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Protein

Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene

Hmgcs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylglutaryl-CoA synthase, cytoplasmic (Hmgcs1), Hydroxymethylglutaryl-CoA synthase, mitochondrial (Hmgcs2)
  3. 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmgcr), 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmgcr), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Hmgcr), 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmgcr)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Proton donor/acceptorPROSITE-ProRule annotation1
Active sitei129Acyl-thioester intermediatePROSITE-ProRule annotation1
Active sitei264Proton donor/acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:Hmgcs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:107592. Hmgcs1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137481 – 520Hydroxymethylglutaryl-CoA synthase, cytoplasmicAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei46N6-acetyllysineBy similarity1
Modified residuei273N6-acetyllysineBy similarity1
Modified residuei495PhosphoserineCombined sources1
Modified residuei516PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8JZK9.
MaxQBiQ8JZK9.
PaxDbiQ8JZK9.
PRIDEiQ8JZK9.

2D gel databases

REPRODUCTION-2DPAGEQ8JZK9.

PTM databases

iPTMnetiQ8JZK9.
PhosphoSitePlusiQ8JZK9.
SwissPalmiQ8JZK9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000093930.
CleanExiMM_HMGCS1.
GenevisibleiQ8JZK9. MM.

Interactioni

Protein-protein interaction databases

BioGridi229007. 1 interactor.
IntActiQ8JZK9. 2 interactors.
MINTiMINT-1869354.
STRINGi10090.ENSMUSP00000136944.

Structurei

3D structure databases

ProteinModelPortaliQ8JZK9.
SMRiQ8JZK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiKOG1393. Eukaryota.
COG3425. LUCA.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiQ8JZK9.
KOiK01641.
OMAiQKEGNDR.
OrthoDBiEOG091G0791.
PhylomeDBiQ8JZK9.
TreeFamiTF105361.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8JZK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG
60 70 80 90 100
LGQARMGFCT DREDINSLCL TVVQKLMERH SLSYDCIGRL EVGTETIIDK
110 120 130 140 150
SKSVKSNLMQ LFEESGNTDI EGIDTTNACY GGTAAVFNAV NWVESSSWDG
160 170 180 190 200
RYALVVAGDI AIYATGNARP TGGVGAVALL IGPNAPLIFD RGLRGTHMQH
210 220 230 240 250
AYDFYKPDML SEYPVVDGKL SIQCYLSALD RCYSVYRKKI RAQWQKEGKD
260 270 280 290 300
KDFTLNDFGF MIFHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA
310 320 330 340 350
FGDVKLEDTY FDRDVEKAFM KASSELFNQK TKASLLVSNQ NGNMYTSSVY
360 370 380 390 400
GSLASVLAQY SPQQLAGKRV GVFSYGSGLA ATLYSLKVTQ DATPGSALDK
410 420 430 440 450
ITASLCDLKS RLDSRTCVAP DVFAENMKLR EDTHHLANYI PQCSIDSLFE
460 470 480 490 500
GTWYLVRVDE KHRRTYARRP FTNDHSLDEG MGLVHSNTAT EHIPSPAKKV
510 520
PRLPATSAES ESAVISNGEH
Length:520
Mass (Da):57,569
Last modified:October 1, 2002 - v1
Checksum:iE28F8772CF64D85C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC023851 mRNA. Translation: AAH23851.1.
BC029693 mRNA. Translation: AAH29693.1.
BC034317 mRNA. Translation: AAH34317.1.
AK031297 mRNA. Translation: BAC27338.1.
AK044835 mRNA. Translation: BAC32112.1.
AK045094 mRNA. Translation: BAC32218.1.
AK135551 mRNA. Translation: BAE22579.1.
CCDSiCCDS56901.1.
RefSeqiNP_001278368.1. NM_001291439.1.
NP_666054.2. NM_145942.5.
XP_006517641.1. XM_006517578.1.
UniGeneiMm.61526.

Genome annotation databases

EnsembliENSMUST00000179869; ENSMUSP00000136944; ENSMUSG00000093930.
GeneIDi208715.
KEGGimmu:208715.
UCSCiuc007rzv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC023851 mRNA. Translation: AAH23851.1.
BC029693 mRNA. Translation: AAH29693.1.
BC034317 mRNA. Translation: AAH34317.1.
AK031297 mRNA. Translation: BAC27338.1.
AK044835 mRNA. Translation: BAC32112.1.
AK045094 mRNA. Translation: BAC32218.1.
AK135551 mRNA. Translation: BAE22579.1.
CCDSiCCDS56901.1.
RefSeqiNP_001278368.1. NM_001291439.1.
NP_666054.2. NM_145942.5.
XP_006517641.1. XM_006517578.1.
UniGeneiMm.61526.

3D structure databases

ProteinModelPortaliQ8JZK9.
SMRiQ8JZK9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229007. 1 interactor.
IntActiQ8JZK9. 2 interactors.
MINTiMINT-1869354.
STRINGi10090.ENSMUSP00000136944.

PTM databases

iPTMnetiQ8JZK9.
PhosphoSitePlusiQ8JZK9.
SwissPalmiQ8JZK9.

2D gel databases

REPRODUCTION-2DPAGEQ8JZK9.

Proteomic databases

EPDiQ8JZK9.
MaxQBiQ8JZK9.
PaxDbiQ8JZK9.
PRIDEiQ8JZK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000179869; ENSMUSP00000136944; ENSMUSG00000093930.
GeneIDi208715.
KEGGimmu:208715.
UCSCiuc007rzv.2. mouse.

Organism-specific databases

CTDi3157.
MGIiMGI:107592. Hmgcs1.

Phylogenomic databases

eggNOGiKOG1393. Eukaryota.
COG3425. LUCA.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiQ8JZK9.
KOiK01641.
OMAiQKEGNDR.
OrthoDBiEOG091G0791.
PhylomeDBiQ8JZK9.
TreeFamiTF105361.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00102.
ReactomeiR-MMU-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiQ8JZK9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000093930.
CleanExiMM_HMGCS1.
GenevisibleiQ8JZK9. MM.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMCS1_MOUSE
AccessioniPrimary (citable) accession number: Q8JZK9
Secondary accession number(s): Q3UXI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.