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Q8JZ13

- NSP1_ROTB4

UniProt

Q8JZ13 - NSP1_ROTB4

Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain Cow/United States/B641/198? G6-P7[5]-Ix-Rx-Cx-Mx-A3-Nx-Tx-Ex-Hx) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri42 – 7938Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
    3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 1
    Short name:
    NSP1
    Alternative name(s):
    NCVP2
    Non-structural RNA-binding protein 53
    Short name:
    NS53
    OrganismiRotavirus A (strain Cow/United States/B641/198? G6-P7[5]-Ix-Rx-Cx-Mx-A3-Nx-Tx-Ex-Hx) (RV-A)
    Taxonomic identifieri10928 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiBos taurus (Bovine) [TaxID: 9913]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-KW
    2. host cytoskeleton Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi54 – 541C → A: 90% loss of IRF3 binding. Complete loss of IRF3 degradation. 1 Publication
    Mutagenesisi79 – 791H → L: 95% loss of IRF3 binding. Complete loss of IRF3 degradation. 1 Publication
    Mutagenesisi136 – 1361H → L: 70% loss of IRF3 binding. No effect on IRF3 degradation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Non-structural protein 1PRO_0000367819Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8181RNA-bindingBy similarityAdd
    BLAST
    Regioni82 – 17695Important for cytoskeleton localizationAdd
    BLAST
    Regioni316 – 491176Interaction with IRF3Add
    BLAST

    Domaini

    The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity.

    Sequence similaritiesi

    Belongs to the rotavirus A NSP1 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri42 – 7938Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR002148. Rotavirus_NSP1.
    [Graphical view]
    PfamiPF00981. Rota_NS53. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8JZ13-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATFKDACYH YKKLNKLNSL VLKLGANDEW RPAPVTKYKG WCLDCCQYTN    50
    LTYCRGCALY HVCQWCSQYN RCFLDEEPHL LRMRTFKDVV TKEDIEGLLT 100
    MYETLFPINE KLVNKFINSV KQRKCRNEYL LEWYNHLLMP ITLQALTINL 150
    EDNVYYMFGY YDCMEHENQT PFQFVNLLEK YDKLLLDDRN FHRMSHLPVI 200
    LQQEYALRYF SKSRFLSKGK KRLSRSDFSD NLMEDRHSPT SLMQVVRNCI 250
    SIHIDDCEWN KACTLIVDAR NYISIMNSSY TEHYSVSQRC KLFTKYKFGI 300
    VSKLVKPNYI FSSHESCALN VHNCKWCQIN NHYKVWEDFR LRKIYNNVMD 350
    FIRALVKSNV NVGHCSSQES VYKYVPDLFL ICKTEKWSEA VEMLFNYLEP 400
    VNVNGTEYVL LDYEVNWEVR GLVMQNMDGK VPRILNMNDT KKILSAMIFD 450
    WFDTRYMRET PMTTSTTNQL RTLNKRNELI DEYDLELSDV E 491
    Length:491
    Mass (Da):58,600
    Last modified:October 1, 2002 - v1
    Checksum:iB91058CEC1D07E54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF458087 mRNA. Translation: AAM21601.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF458087 mRNA. Translation: AAM21601.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002148. Rotavirus_NSP1.
    [Graphical view ]
    Pfami PF00981. Rota_NS53. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Translational regulation of rotavirus gene expression."
      Mitzel D.N., Weisend C.M., White M.W., Hardy M.E.
      J. Gen. Virol. 84:383-391(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Interferon regulatory factor 3 is a cellular partner of rotavirus NSP1."
      Graff J.W., Mitzel D.N., Weisend C.M., Flenniken M.L., Hardy M.E.
      J. Virol. 76:9545-9550(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN IRF3.
    3. "Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
      Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
      J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-54; HIS-79 AND HIS-136.

    Entry informationi

    Entry nameiNSP1_ROTB4
    AccessioniPrimary (citable) accession number: Q8JZ13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3