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Q8JZ13 (NSP1_ROTB4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain Cow/United States/B641/198? G6-P7[5]-Ix-Rx-Cx-Mx-A3-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifier10928 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation. Ref.3

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity. Ref.2

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Non-structural protein 1
PRO_0000367819

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17695Important for cytoskeleton localization
Region316 – 491176Interaction with IRF3

Experimental info

Mutagenesis541C → A: 90% loss of IRF3 binding. Complete loss of IRF3 degradation. Ref.3
Mutagenesis791H → L: 95% loss of IRF3 binding. Complete loss of IRF3 degradation. Ref.3
Mutagenesis1361H → L: 70% loss of IRF3 binding. No effect on IRF3 degradation. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8JZ13 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: B91058CEC1D07E54

FASTA49158,600
        10         20         30         40         50         60 
MATFKDACYH YKKLNKLNSL VLKLGANDEW RPAPVTKYKG WCLDCCQYTN LTYCRGCALY 

        70         80         90        100        110        120 
HVCQWCSQYN RCFLDEEPHL LRMRTFKDVV TKEDIEGLLT MYETLFPINE KLVNKFINSV 

       130        140        150        160        170        180 
KQRKCRNEYL LEWYNHLLMP ITLQALTINL EDNVYYMFGY YDCMEHENQT PFQFVNLLEK 

       190        200        210        220        230        240 
YDKLLLDDRN FHRMSHLPVI LQQEYALRYF SKSRFLSKGK KRLSRSDFSD NLMEDRHSPT 

       250        260        270        280        290        300 
SLMQVVRNCI SIHIDDCEWN KACTLIVDAR NYISIMNSSY TEHYSVSQRC KLFTKYKFGI 

       310        320        330        340        350        360 
VSKLVKPNYI FSSHESCALN VHNCKWCQIN NHYKVWEDFR LRKIYNNVMD FIRALVKSNV 

       370        380        390        400        410        420 
NVGHCSSQES VYKYVPDLFL ICKTEKWSEA VEMLFNYLEP VNVNGTEYVL LDYEVNWEVR 

       430        440        450        460        470        480 
GLVMQNMDGK VPRILNMNDT KKILSAMIFD WFDTRYMRET PMTTSTTNQL RTLNKRNELI 

       490 
DEYDLELSDV E 

« Hide

References

[1]"Translational regulation of rotavirus gene expression."
Mitzel D.N., Weisend C.M., White M.W., Hardy M.E.
J. Gen. Virol. 84:383-391(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Interferon regulatory factor 3 is a cellular partner of rotavirus NSP1."
Graff J.W., Mitzel D.N., Weisend C.M., Flenniken M.L., Hardy M.E.
J. Virol. 76:9545-9550(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN IRF3.
[3]"Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-54; HIS-79 AND HIS-136.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF458087 mRNA. Translation: AAM21601.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTB4
AccessionPrimary (citable) accession number: Q8JZ13
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families