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Q8JZ13

- NSP1_ROTB4

UniProt

Q8JZ13 - NSP1_ROTB4

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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain Cow/United States/B641/198? G6-P7[5]-Ix-Rx-Cx-Mx-A3-Nx-Tx-Ex-Hx) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain Cow/United States/B641/198? G6-P7[5]-Ix-Rx-Cx-Mx-A3-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifieri10928 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi54 – 541C → A: 90% loss of IRF3 binding. Complete loss of IRF3 degradation. 1 Publication
Mutagenesisi79 – 791H → L: 95% loss of IRF3 binding. Complete loss of IRF3 degradation. 1 Publication
Mutagenesisi136 – 1361H → L: 70% loss of IRF3 binding. No effect on IRF3 degradation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Non-structural protein 1PRO_0000367819Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8181RNA-bindingBy similarityAdd
BLAST
Regioni82 – 17695Important for cytoskeleton localizationAdd
BLAST
Regioni316 – 491176Interaction with IRF3Add
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity.

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938Sequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8JZ13-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATFKDACYH YKKLNKLNSL VLKLGANDEW RPAPVTKYKG WCLDCCQYTN
60 70 80 90 100
LTYCRGCALY HVCQWCSQYN RCFLDEEPHL LRMRTFKDVV TKEDIEGLLT
110 120 130 140 150
MYETLFPINE KLVNKFINSV KQRKCRNEYL LEWYNHLLMP ITLQALTINL
160 170 180 190 200
EDNVYYMFGY YDCMEHENQT PFQFVNLLEK YDKLLLDDRN FHRMSHLPVI
210 220 230 240 250
LQQEYALRYF SKSRFLSKGK KRLSRSDFSD NLMEDRHSPT SLMQVVRNCI
260 270 280 290 300
SIHIDDCEWN KACTLIVDAR NYISIMNSSY TEHYSVSQRC KLFTKYKFGI
310 320 330 340 350
VSKLVKPNYI FSSHESCALN VHNCKWCQIN NHYKVWEDFR LRKIYNNVMD
360 370 380 390 400
FIRALVKSNV NVGHCSSQES VYKYVPDLFL ICKTEKWSEA VEMLFNYLEP
410 420 430 440 450
VNVNGTEYVL LDYEVNWEVR GLVMQNMDGK VPRILNMNDT KKILSAMIFD
460 470 480 490
WFDTRYMRET PMTTSTTNQL RTLNKRNELI DEYDLELSDV E
Length:491
Mass (Da):58,600
Last modified:October 1, 2002 - v1
Checksum:iB91058CEC1D07E54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF458087 mRNA. Translation: AAM21601.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF458087 mRNA. Translation: AAM21601.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002148. Rotavirus_NSP1.
[Graphical view ]
Pfami PF00981. Rota_NS53. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Translational regulation of rotavirus gene expression."
    Mitzel D.N., Weisend C.M., White M.W., Hardy M.E.
    J. Gen. Virol. 84:383-391(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Interferon regulatory factor 3 is a cellular partner of rotavirus NSP1."
    Graff J.W., Mitzel D.N., Weisend C.M., Flenniken M.L., Hardy M.E.
    J. Virol. 76:9545-9550(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN IRF3.
  3. "Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
    Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
    J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-54; HIS-79 AND HIS-136.

Entry informationi

Entry nameiNSP1_ROTB4
AccessioniPrimary (citable) accession number: Q8JZ13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3