Q8JU57 (VP4_AQRVC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer capsid protein VP4

Gene names

Name:

S6

Organism

Aquareovirus C (isolate Golden shiner/USA/GSRV/1977) (AQRV-C) [Reference proteome]

Taxonomic identifier

185783 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Aquareovirus › Aquareovirus C

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interacts with VP7 to form the outer icosahedral capsid with an incomplete T=13 symmetry, about 80 nm in diameter, and consisting of 200 VP4-VP7 trimers. Myristoylated N-terminal peptide may be released in the endosome and involved in permeabilization and delivery of transcriptionally active viral particles into the host cell cytoplasm Potential.
Subunit structure	Interacts with VP6 and VP7 By similarity.
Subcellular location	Virion Potential.
Post-translational modification	Cleaved during the endosomal proteolytic disassembly of the outer capsid By similarity. N-terminally myristoylated. This acylation is essential for the membrane fusion activity By similarity.
Sequence similarities	Belongs to the aquareoviridae outer capsid VP4 protein family.

Ontologies

Keywords
Biological process	Viral penetration into host cytoplasm Viral penetration via permeabilization of host organellar membrane Virus entry into host cell
Cellular component	Virion
Molecular function	Capsid protein T=13 icosahedral capsid protein
PTM	Glycoprotein Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	T=13 icosahedral viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cell surface binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 648	647	Outer capsid protein VP4		PRO_0000404187
Regions
	Compositional bias	112 – 115	4	Poly-Thr
Sites
	Site	42 – 43	2	Cleavage By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine; by host By similarity
	Glycosylation	12	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	311	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	312	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	390	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	453	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	492	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8JU57 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A6FA4DA4164826D2	FASTA	648	68,555
	10 20 30 40 50 60 MGNVQTSVNT YNITGDGNSF TPTSDMTSTA APAIDLKPGV LNPTGKLWRP VGTSVATIDS 70 80 90 100 110 120 LAIVSDRFGQ YSFVNEGMRE TFSKALFDIN MWQPLFQATK TGCGPIVLSS FTTTTSGYVG 130 140 150 160 170 180 ATAGDALDNP VTNGVFISTV QVMNLQRTIA ARMRDVALWQ QHLDTAMTML TPDISAGSAS 190 200 210 220 230 240 CNWKSLLAFA KDILPLDNLC LTYPNEFYNV AIHRYPALKP GNPDTKLPDA QAHPLGEVAG 250 260 270 280 290 300 AFNAATSEVG SLVGSSSTLS QAISTMAGKD LDLIEADTPL PVSVFTPSLA PRSYRPAFIK 310 320 330 340 350 360 PEDAKWIAEF NNSSLIRKTL TYSGATYAVQ LGPGPTRVID MNAMIDSVLT LDVSGTILPY 370 380 390 400 410 420 DTNPDLSTSV PAFVLIQTSV PIQQVTTAAN ITAITVVSAA GASAINLAIN VRGQPRFNML 430 440 450 460 470 480 HLQATFERET ITGIPYIYGL GTFLIPSPTS SSNFSNPTLM DGLLTVTPVL LRETTYKGEV 490 500 510 520 530 540 VDAIVPATVM ANQTSEEVAS ALANDAIVLV SNHLNKLANV VGDAIPVASK TDDSATSAIV 550 560 570 580 590 600 SRLAVQHKLS QVGQTSPTPP DYPLLWRRAK RAASMFVSNP SLALQVGIPV LTQSGMLSAL 610 620 630 640 TSGVGTALRT GSLGKGVTDA SEKLRARQSL TVAKQAFFDQ IGSLWPGK

References

[1]

"Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae)." Attoui H., Fang Q., Mohd Jaafar F., Cantaloube J.F., Biagini P., de Micco P., de Lamballerie X. J. Gen. Virol. 83:1941-1951(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF403403 Genomic RNA. Translation: AAM92749.1.
RefSeq	NP_938065.1. NC_005171.1.
3D structure databases
ProteinModelPortal	Q8JU57.
SMR	Q8JU57. Positions 10-42.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2648334.
Family and domain databases
Gene3D	1.10.2040.10. 2 hits. 2.60.120.420. 1 hit.
InterPro	IPR009113. Mu1/VP4. IPR015962. Mu1_membr_pen_a-bundle_sub2. IPR015960. Mu1_membr_pen_b-sand_sub. [Graphical view]
Pfam	PF05993. Reovirus_M2. 1 hit. [Graphical view]
SUPFAM	SSF69908. Mu1_membr_pen. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VP4_AQRVC

Accession

Primary (citable) accession number: Q8JU57

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program