Q8JU42 (Q8JU42_WNV) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Polyprotein EMBL AAM81753.1
Organism	West Nile virus (WNV) EMBL AAM81753.1
Taxonomic identifier	11082 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus › Japanese encephalitis virus group
Virus host	Aedes [TaxID: 7158] Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Amblyomma variegatum (Tropical bont tick) [TaxID: 34610] Hyalomma marginatum [TaxID: 34627] Rhipicephalus [TaxID: 34630] Culex [TaxID: 53527] Mansonia uniformis [TaxID: 308735] Mimomyia [TaxID: 308737]

Protein attributes

Sequence length	3433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes By similarity. SAAS SAAS013754
Catalytic activity	ATP + H2O = ADP + phosphate. SAAS SAAS007094 NTP + H2O = NDP + phosphate. SAAS SAAS007094 Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS007094
Subcellular location	Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein By similarity SAAS SAAS013754.
Sequence similarities	Contains 1 RdRp catalytic domain. SAAS SAAS007094 Contains 1 helicase ATP-binding domain. SAAS SAAS007094 Contains 1 helicase C-terminal domain. SAAS SAAS007094

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host SAAS SAAS013754 Fusion of virus membrane with host endosomal membrane SAAS SAAS013754 Fusion of virus membrane with host membrane Host-virus interaction Initiation of viral infection RNA replication SAAS SAAS007094 Viral attachment to host cell SAAS SAAS013754 Viral penetration into host cytoplasm Virus endocytosis by host
Cellular component	Host endoplasmic reticulum SAAS SAAS013754 Host membrane SAAS SAAS013754 Membrane Viral envelope protein SAAS SAAS013754 Virion
Domain	Transmembrane Transmembrane helix SAAS SAAS007094
Ligand	ATP-binding Nucleotide-binding
Molecular function	Capsid protein SAAS SAAS007094 Helicase SAAS SAAS007094 Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase SAAS SAAS007094 Transferase
PTM	Disulfide bond SAAS SAAS013754
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: InterPro virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro mRNA (nucleoside-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8JU42 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1DFFCCDB2174B7EE	FASTA	3,433	381,210
	10 20 30 40 50 60 MSKKPGGPGK SRAVNMLKRG MPRVLSLIGL KRAMLSLIDG KGPIRFVLAL LAFFRFTAIA 70 80 90 100 110 120 PTRAVLDRWR GVNKQTAMKH LLSFKKELGT LTSAINRRSS KQKKRGGKTG IAVMIGLIAS 130 140 150 160 170 180 VGAVTLSNFQ GKVMMTVNAT DVTDVITIPT AAGKNLCIVR AMDVGYMCDD TITYECPVLS 190 200 210 220 230 240 AGNDPEDIDC WCTKSAVYVR YGRCTKTRHS RRSRRSLTVQ THGESTLANK KGAWMDSTKA 250 260 270 280 290 300 TRYLVKTESW ILRNPGYALV AAVIGWMLGS NTMQRVVFVV LLLLVAPAYS FNCLGMSNRD 310 320 330 340 350 360 FLEGVSGATW VDLVLEGDSC VTIMSKDKPT IDVKMMNMEA ANLAEVRSYC YLATVSDLST 370 380 390 400 410 420 KAACPTMGEA HNDKRADPAF VCRQGVVDRG WGNGCGLFGK GSIDTCAKFA CSTKATGRTI 430 440 450 460 470 480 LKENIKYEVA IFVHGPTTVE SHGNYSTQIG ATQAGRFSIT PAAPSYTLKL GEYGEVTVDC 490 500 510 520 530 540 EPRSGIDTNA YYVMTVGTKT FLVHREWFMD LNLPWSSAGS TVWRNRETLM EFEEPHATKQ 550 560 570 580 590 600 SVIALGSQEG ALHQALAGAI PVEFSSNTVK LTSGHLKCRV KMEKLQLKGT TYGVCSKAFK 610 620 630 640 650 660 FLGTPADTGH GTVVLELQYT GTDGPCKVPI SSVASLNDLT PVGRLVTVNP FVSVATANAK 670 680 690 700 710 720 VLIELEPPFG DSYIVVGRGE QQINHHWHKS GSSIGKAFTT TLKGAQRLAA LGDTAWDFGS 730 740 750 760 770 780 VGGVFTSVGK AVHQVFGGAF RSLFGGMSWI TQGLLGALLL WMGINARDRS IALTFLAVGG 790 800 810 820 830 840 VLLFLSVNVH ADTGCAIDIS RQELRCGSGV FIHNDVEAWM DRYKYYPETP QGLAKIIQKA 850 860 870 880 890 900 HKEGVCGLRS VSRLEHQMWE SVKDELNTLL KENGVDLSVV VEKQEGMYKS APKRLTATTE 910 920 930 940 950 960 KLEIGWKAWG KSILFAPELA NNTFVVDGPE TKECPTQNRA WNSLEVEDFG FGLTSTRMFL 970 980 990 1000 1010 1020 KVRESNTTEC DSKIIGTAVK NNLAIHSDLS YWIESRLNDT WKLERAVLGE VKSCTWPETH 1030 1040 1050 1060 1070 1080 TLWGDGILES DLIIPVTLAG PRSNHNRRPG YKTQNQGPWD EGRVEIDFDY CPGTTVTLSE 1090 1100 1110 1120 1130 1140 SCGHRGPATR TTTESGKLIT DWCCRSCTLP PLRYQTDSGC WYGMEIRPQR HDEKTLVQSQ 1150 1160 1170 1180 1190 1200 VNAYNADMID PFQLGLLVVF LATQEVLRKR WTAKISMPAI LIALLVLVFG GITYTDVLRY 1210 1220 1230 1240 1250 1260 VILVGAAFAE SNSGGDVVHL ALMATFKIQP VFMVASFLKA RWTNQENILL MLAAVFFQMA 1270 1280 1290 1300 1310 1320 YHDARQILLW EIPDVLNSLA VAWMILRAIT FTTTSNVVVP LLALLTPGLR CLNLDVYRIL 1330 1340 1350 1360 1370 1380 LLMVGIGSLI REKRSAAAKK KGASLLCLAL ASTGLFNPMI LAAGLIACDP NRKRGWPATE 1390 1400 1410 1420 1430 1440 VMTAVGLMFA IVGGLAELDI DSMAIPMTIA GLMFAAFVIS GKSTDMWIER TADISWESDA 1450 1460 1470 1480 1490 1500 EITGSSERVD VRLDDDGNFQ LMNDPGAPWK IWMLRMACLA ISAYTPWAIL PSVIGFWITL 1510 1520 1530 1540 1550 1560 QYTKRGGVLW DTPSPKEYKK GDTTTGVYRI MTRGLLGSYQ AGAGVMVEGV FHTLWHTTKG 1570 1580 1590 1600 1610 1620 AALMSGEGRL DPYWGSVKED RLCYGGPWKL QHKWNGQDEV QMIVVEPGKN VKNVQTKPGV 1630 1640 1650 1660 1670 1680 FKTPEGEIGA VTLDFPTGTS GSPIVDKNGD VIGLYGNGVI MPNGSYISAI VQGERMDEPI 1690 1700 1710 1720 1730 1740 PAGFEPEMLR KKQITVLDLH PGAGKTRRIL PQIIKEAINR RLRTAVLAPT RVVAAEMAEA 1750 1760 1770 1780 1790 1800 LRGLPIRYQT SAVTREHNGN EIVDVMCHAT LTHRLMSPHR VPNYNLFVMD EAHFTDPASI 1810 1820 1830 1840 1850 1860 AARGYISTKV ELGEAAAIFM TATPPGTSDP FPESNSPISD LQTEIPDRAW NSGYEWITEY 1870 1880 1890 1900 1910 1920 IGKTVWFVPS VKMGNEIALC LQRAGKKVVQ LNRKSYETEY PKCKNDDWDF VITTDISEMG 1930 1940 1950 1960 1970 1980 ANFKASRVID SRKSVKPTII TEGEGRVILG EPSAVTAASA AQRRGRIGRN PSQVGDEYCY 1990 2000 2010 2020 2030 2040 GGHTNEDDSN FAHWTEARIM LDNINMPNGL IAQFYQPERE KVYTMDGEYR LRGEERKNFL 2050 2060 2070 2080 2090 2100 ELLRTADLPV WLAYKVAAAG VSYHDRRWCF DGPRTNTILE DNNEVEVITK LGERKILRPR 2110 2120 2130 2140 2150 2160 WIDARVYSDH QALKAFKDFA SGKRSQIGLI EVLGKMPEHF MGKTWEALDT MYVVATAEKG 2170 2180 2190 2200 2210 2220 GRAHRMALEE LPDALQTIAL IALLSVMTMG VFFLLMQRKG IGKIGLGGVV LGVATFFCWM 2230 2240 2250 2260 2270 2280 AEVPGTKIAG MLLLSLLLMI VLIPEPEKQR SQTDNQLAVF LICVMTLVSA VAANEMGWLD 2290 2300 2310 2320 2330 2340 KTKSDISSLF GQRIEVKENF SMGEFLLDLR PATAWSLYAV TTAVLTPLLK HLITSDYINT 2350 2360 2370 2380 2390 2400 SLTSINVQAS ALFTLARGFP FVDVGVSALL LAAGCWGQVT LTVTVTAATL LFCHYAYMVP 2410 2420 2430 2440 2450 2460 GWQAEAMRSA QRRTAAGIMK NAVVDGIVAT DVPELERTTP IMQKKVGQIM LILVSLAAVV 2470 2480 2490 2500 2510 2520 VNPSVKTVRE AGILITAAAV TLWENGASSV WNATTAIGLC HIMRGGWLSC LSITWTLIKN 2530 2540 2550 2560 2570 2580 MDKPGLKRGG AKGRTLGEVW KERLNQMTKE EFTRYRKEAI IEVDRSAAKH ARKEGNVTGG 2590 2600 2610 2620 2630 2640 HPVSRGTAKL RWLVERRFLE PVGKVIDLGC GRGGWCYYMA TQKRVQEVRG YTKGGPGHEE 2650 2660 2670 2680 2690 2700 PQLVQSYGWN IVTMKSGVDV FYRPSECCDT LLCDIGESSS SAEVEEHRTI RVLEMVEDWL 2710 2720 2730 2740 2750 2760 HRGPREFCVK VLCPYMPKVI EKMELLQRRY GGGLVRNPLS RNSTHEMYWV SRASGNVVHS 2770 2780 2790 2800 2810 2820 VNMTSQVLLG RMEKRTWKGP QYEEDVNLGS GTRAVGKPLL NSDTSKIKNR IERLRREYSS 2830 2840 2850 2860 2870 2880 TWHHDENHPY RTWNYHGSYD VKPTGSASSL VNGVVRLLSK PWDTITNVTT MAMTDTTPFG 2890 2900 2910 2920 2930 2940 QQRVFKEKVD TKAPEPPEGV KYVLNETTNW LWAFLAREKR PRMCSREEFI RKVNSNAALG 2950 2960 2970 2980 2990 3000 AMFEEQNQWR SAREAVEDPK FWEMVDEERE AHLRGECHTC IYNMMGKREK KPGEFGKAKG 3010 3020 3030 3040 3050 3060 SRAIWFMWLG ARFLEFEALG FLNEDHWLGR KNSGGGVEGL GLQKLGYILR EVGTRPGGKI 3070 3080 3090 3100 3110 3120 YADDTAGWDT RITRADLENE AKVLELLDGE HRRLARAIIE LTYRHKVVKV MRPAADGRTV 3130 3140 3150 3160 3170 3180 MDVISREDQR GSGQVVTYAL NTFTNLAVQL VRMMEGEGVI GPDDVEKLTK GKGPKVRTWL 3190 3200 3210 3220 3230 3240 FENGEERLSR MAVSGDDCVV KPLDDRFATS LHFLNAMSKV RKDIQEWKPS TGWYDWQQVP 3250 3260 3270 3280 3290 3300 FCSNHFTELI MKDGRTLVVP CRGQDELVGR ARISPGAGWN VRDTACLAKS YAQMWLLLYF 3310 3320 3330 3340 3350 3360 HRRDLRLMAN AICSAVPVNW VPTGRTTWSI HAGGEWMTTE DMLEVWNRVW IEENEWMEDK 3370 3380 3390 3400 3410 3420 TPVEKWSDVP YSGKREDIWC GSLIGTRARA TWAENIQVAI NQVRAIIGDE KYVDYMSSLK 3430 RYEDTTLVED TVL

References

[1]	"Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle East." Lanciotti R.S., Ebel G.D., Deubel V., Kerst A.J., Murri S., Meyer R., Bowen M., McKinney N., Morrill W.E., Crabtree M.B., Kramer L.D., Roehrig J.T. Virology 298:96-105(2002) [PubMed: 12093177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF404757 Genomic RNA. Translation: AAM81753.1.
3D structure databases
HSSP	HSSP built from PDB template 1L9K based on UniProtKB Q88653.
ProteinModelPortal	Q8JU42.
SMR	Q8JU42. Positions 25-97, 291-693, 1424-1467, 1506-2124, 2534-2795, 2802-3427.
ModBase	Search...
Protein family/group databases
MEROPS	S07.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011492. DEAD_Flavivir. IPR000069. Env_glycoprot_M_flavivir. IPR013755. Flav_glyE_cen_dom_subdom1. IPR013756. Flav_glyE_cen_dom_subdom2. IPR013754. Flav_glyE_dim. IPR001122. Flavi_capsidC. IPR001157. Flavi_NS1. IPR000752. Flavi_NS2A. IPR000487. Flavi_NS2B. IPR000404. Flavi_NS4A. IPR001528. Flavi_NS4B. IPR002535. Flavi_propep. IPR000336. Flv_glyE_Ig-like. IPR014412. Gen_Poly_FLV. IPR011999. GlycoprotE_cen/dimer_Flavivir. IPR011998. GlycoprotE_cen/dimer_vir. IPR001650. Helicase_C. IPR014756. Ig_E-set. IPR009003. Pept_cys/ser_Trypsin-like. IPR001850. Peptidase_S7. IPR000208. RNA-dir_pol_flavivirus. IPR007094. RNA-dir_pol_PSvirus. IPR002877. rRNA_MeTrfase_RrmJ/FtsJ. [Graphical view]
Gene3D	G3DSA:3.30.387.10. Flav_glyE_cen_1. 1 hit. G3DSA:3.30.67.10. Flav_glyE_cen_2. 1 hit. G3DSA:2.60.98.10. Flav_glyE_dim. 1 hit. G3DSA:2.60.40.350. Flv_glyE_Ig-like. 1 hit.
Pfam	PF01003. Flavi_capsid. 1 hit. PF07652. Flavi_DEAD. 1 hit. PF02832. Flavi_glycop_C. 1 hit. PF00869. Flavi_glycoprot. 1 hit. PF01004. Flavi_M. 1 hit. PF00948. Flavi_NS1. 1 hit. PF01005. Flavi_NS2A. 1 hit. PF01002. Flavi_NS2B. 1 hit. PF01350. Flavi_NS4A. 1 hit. PF01349. Flavi_NS4B. 1 hit. PF00972. Flavi_NS5. 1 hit. PF01570. Flavi_propep. 1 hit. PF01728. FtsJ. 1 hit. PF00271. Helicase_C. 1 hit. PF00949. Peptidase_S7. 1 hit. [Graphical view]
PIRSF	PIRSF003817. Gen_Poly_FLV. 1 hit.
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF56983. Flavi_glycoprotE. 1 hit. SSF81296. Ig_E-set. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8JU42_WNV

Accession

Primary (citable) accession number: Q8JU42

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)