Reviewed, UniProtKB/Swiss-Prot Q8JTG9 (L_ABLVH)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Australian bat lyssavirus (isolate Human/AUS/1998) (ABLV) [Complete proteome]

Taxonomic identifier

446562 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus

Virus host

Pteropus alecto (Black flying fox) [TaxID: 9402]
Pteropus poliocephalus (Grey-headed flying fox) [TaxID: 9403]
Homo sapiens (Human) [TaxID: 9606]
Pteropus scapulatus (Little red flying fox) [TaxID: 94117]
Pteropus conspicillatus (Spectacled flying fox) [TaxID: 328804]
Saccolaimus [TaxID: 446909]

Protein attributes

Sequence length	2127 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviruses protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2127	2127	Large structural protein		PRO_0000294414
Regions
	Domain	611 – 799	189	RdRp catalytic
	Region	1562 – 2127	566	Interaction with P protein By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8JTG9-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: E3777CFCDA404F4E	FASTA	2,127	242,676
	10 20 30 40 50 60 MIDPGEVYDD PIDPVEPEPE LKNNTVIPNI LRNSDYNLNS PLIEDSARLM LEWLTTGNRP 70 80 90 100 110 120 GRLTLTDNCI RSYKTLKCYF RKVDVGSLKV GGLAAQAMIS LWLHGEHSES NRSRKCLSDL 130 140 150 160 170 180 TQFYQKSSPI EKLLNYTLGN RGLRIPPEGV LFCLKKVDYD RAFGRYLANI YASYLFFHVI 190 200 210 220 230 240 ILYMNALDWD EEKTILALWK DLSSVDIKKD QVKFRDPIWG SLIVTKDFVY SQNANCLFDR 250 260 270 280 290 300 NYTLMLKDLF LSRFNSLLIL LSPPEPRYSD DLISQLCQLY IAGDNVLSMC GNSGYDVIKM 310 320 330 340 350 360 LEPYVVNSLV QRAEGFRPLI HSLGDFPLFI RDKVGQLEGT FGPSARKFFQ VLDQFDNIHD 370 380 390 400 410 420 LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH IKKVIDGTYQ ECLASDLAKR VLRWGFDKYS 430 440 450 460 470 480 KWYLDPKLLA QDHPLTPYIR TQTWPPKHIV DLVGNTWHRL PITQIFEIPE SMDPSEILDD 490 500 510 520 530 540 KSHSLTRTKL ASWLAENRGG PVPSEKVIIT ALSKPPVNPR EFLKSIDLGG LPDDDLIIGL 550 560 570 580 590 600 KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 610 620 630 640 650 660 TGQGLQDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDQ VFGLKKVFSR THEFFQKSWV 670 680 690 700 710 720 YYSDRSDLIG LWEDQIYCLD MSDGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 730 740 750 760 770 780 RTKILAQGDN QVLCPTYMLS SGLSQEGLLY ELESISRNAL SIYRAIEDGA SKLGLIIKKE 790 800 810 820 830 840 ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 850 860 870 880 890 900 SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAD GDDFLLAMSR IIYLDPSLGG 910 920 930 940 950 960 VSGMSLGRFH IRQFSDPVSE GLSFWKEIWS SSSEPWIHSL CQEAGNPDLG DRSLESFTRL 970 980 990 1000 1010 1020 LEDPTTLNIR GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSI 1030 1040 1050 1060 1070 1080 EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRSLEESFY NSEIHGINRM 1090 1100 1110 1120 1130 1140 TQVPQRIGRV WSCSSERADL LREISWGRKV VGTTVPHPSE MLTLLPKSSI SCVCGPTGSE 1150 1160 1170 1180 1190 1200 NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 1210 1220 1230 1240 1250 1260 WFIARDSNLA QTLIKNIISL TGPQFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSICPNL 1270 1280 1290 1300 1310 1320 LSHISVSTDT MSDLTQDGRN FDFMFQPLML YAQTWTSELV QKDIRLKDST FHWHLRCQKC 1330 1340 1350 1360 1370 1380 IRSIDDITLD TSQVFEFPDV SKRISRMVSG AVPQFQKLPE VYLKPGHFDS LCGKDKSRHI 1390 1400 1410 1420 1430 1440 GSAQGLLYSI LVATHDPGYN DGTIFPVNIY SKISPKDYLR GLARGVLIGS SICFLTRMTN 1450 1460 1470 1480 1490 1500 ININRPLELI SGVISYILLR LDNHPSLYVM LRESSLRSEI FSIPQKIPAA YPTTMKEGNR 1510 1520 1530 1540 1550 1560 SVLCYLQHVL RYERDIITSS PENDWLWIFS DFRSTKMTYL TLITYQSHIL LQRIERSLSK 1570 1580 1590 1600 1610 1620 KMRADLRQLS SLMRQVLGGH GEDSLDSGED IQRLLRDALQ RTRWVDQEVR HAAKTMTGDH 1630 1640 1650 1660 1670 1680 SPSKKTSRKA GCSEWICSAQ QVAISTSSNP APVSEMDIRT LSRKLQNPLI SGLRVVQWAT 1690 1700 1710 1720 1730 1740 GAHYKLKPIL DDLNAYPSFC LVVGDGSGGI SRTVLNMFPD AKLVFNSLLE VSDLMASGTH 1750 1760 1770 1780 1790 1800 PLPPSAIMSG GDDIVSRVVD FESIWEKPSD LRNLSTWRYF QSVQSKLNMS YDLIICDAEV 1810 1820 1830 1840 1850 1860 TDIISVNKIT LLMSDFVLSI DGPLDLIFKS YGTMLVNPDY KAIQHLSRAF PKATGYITQL 1870 1880 1890 1900 1910 1920 TSSFSSELYL RFSKRGKFFR DVEYLTSSTL REMSLVLFNC SSPKSELQRA RSLNYQDLIR 1930 1940 1950 1960 1970 1980 GFPEEIVSNP YNEMIITLID SEVESFLVHK MVDDLELQRG TLSKMSIIIA IVIVYSNRVF 1990 2000 2010 2020 2030 2040 NVSKPLSDPV FYPPSDPKIL RHFNICCSTM LYLSTILGDV PNFARLHELY NSPITYYFKK 2050 2060 2070 2080 2090 2100 QIIRGSIYLS WSWSDDTSVF KRVSCNSNLS LSSHWIRLIY KIIKTTRFTG SHVDLSKEVE 2110 2120 KHLKGYNRWI TFNDVRSRSS LLDYSCL

References

[1]	"Sequence analysis of an isolate from a fatal human infection of Australian bat lyssavirus." Warrilow D., Smith I.L., Harrower B., Smith G.A. Virology 297:109-119(2002) [PubMed: 12083841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF418014 Genomic RNA. Translation: AAN05310.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_ABLVH

Accession

Primary (citable) accession number: Q8JTG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)