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Q8JSP9

- HEMA_CVPIA

UniProt

Q8JSP9 - HEMA_CVPIA

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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Porcine hemagglutinating encephalomyelitis virus (strain IAF-404) (HEV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401NucleophileBy similarity
Active sitei326 – 3261Charge relay systemBy similarity
Active sitei329 – 3291Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPorcine hemagglutinating encephalomyelitis virus (strain IAF-404) (HEV)
Taxonomic identifieri230236 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007543: Genome

Subcellular locationi

Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface (By similarity).By similarity

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 424406Hemagglutinin-esterasePRO_0000037150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 65By similarity
Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi113 ↔ 162By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi197 ↔ 276By similarity
Disulfide bondi205 ↔ 249By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi307 ↔ 312By similarity
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi347 ↔ 371By similarity
Glycosylationi358 – 3581N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ8JSP9.
SMRiQ8JSP9. Positions 19-375.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 392374Virion surfaceSequence AnalysisAdd
BLAST
Topological domaini414 – 42411IntravirionSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei393 – 41321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 127121Esterase domain first partBy similarityAdd
BLAST
Regioni128 – 266139Receptor bindingBy similarityAdd
BLAST
Regioni267 – 379113Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JSP9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLLPRFCLV CSIISTFGFQ NPPTNVVSHF NDDWFLFGDS RTDCNHVVKT
60 70 80 90 100
NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY
110 120 130 140 150
EGVNFTPYHA FKCTSVGNND IWMQNKGLFY TQVYKKMAVY RSLTLVNVPY
160 170 180 190 200
VYNGSAQPTA LCKSGSLILN NPAYIAREAN VGDYYYKSEA DFSLSGCDEY
210 220 230 240 250
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH
260 270 280 290 300
YLVLPSGNYL AISNELLLTV PTKAICLNKR KVFTPVQVVD SRWNNARQSD
310 320 330 340 350
NMTAVACQLP YCYFRNSTSN YVGIYDVNHG DAGFTSILSG LLYDSPCFSQ
360 370 380 390 400
QGVFRYDNVS TVWPLFPFGN CPTAASIISS DLPICVYDPL PIILLGILLG
410 420
VAVIVIVVLL LYFMVDNGIR QHYA
Length:424
Mass (Da):47,745
Last modified:October 1, 2002 - v1
Checksum:i8F81920B566EC2A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF481863 Genomic RNA. Translation: AAM76999.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF481863 Genomic RNA. Translation: AAM76999.1 .

3D structure databases

ProteinModelPortali Q8JSP9.
SMRi Q8JSP9. Positions 19-375.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of the 3'-terminal end (8.1 kb) of the genome of porcine haemagglutinating encephalomyelitis virus: comparison with other haemagglutinating coronaviruses."
    Sasseville A.M.-J., Boutin M., Gelinas A.-M., Dea S.
    J. Gen. Virol. 83:2411-2416(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVPIA
AccessioniPrimary (citable) accession number: Q8JSP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3