Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition host IKBKE and TBK1 producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host IKBKE by virus, Inhibition of host innate immune response by virus, Inhibition of host IRF7 by virus, Inhibition of host RLR pathway by virus, Inhibition of host TBK1 by virus, Inhibition of host TLR pathway by virus, Interferon antiviral system evasion, Transcription, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase cofactor VP35
Gene namesi
Name:VP35
ORF Names:REBOVgp2
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007207 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Polymerase cofactor VP35PRO_0000245076Add
BLAST

Post-translational modificationi

Phosphorylated by host IKBKE.By similarity

Interactioni

Subunit structurei

Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein (By similarity). May interact with VP40. Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3 (By similarity).By similarity

Structurei

Secondary structure

1
329
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi210 – 21910Combined sources
Beta strandi223 – 2253Combined sources
Helixi227 – 24115Combined sources
Helixi245 – 25713Combined sources
Helixi262 – 27211Combined sources
Helixi275 – 2784Combined sources
Beta strandi283 – 2853Combined sources
Helixi289 – 2913Combined sources
Helixi294 – 2963Combined sources
Beta strandi299 – 3024Combined sources
Helixi309 – 3113Combined sources
Beta strandi313 – 3186Combined sources
Beta strandi324 – 3285Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KS4X-ray2.40A/B160-329[»]
3KS8X-ray2.40A/B/C/D160-329[»]
3L2AX-ray1.71A204-329[»]
4LG2X-ray2.70A/B/C/D205-329[»]
ProteinModelPortaliQ8JPY0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8JPY0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 329126VP35 IIDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili84 – 11229Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the filoviridae polymerase cofactor VP35 family.PROSITE-ProRule annotationCurated
Contains 1 VP35 IID (interferon inhibitory) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002953. Filo_VP35.
IPR031163. VP35_IID.
[Graphical view]
PfamiPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
PRINTSiPR01240. FILOVP35.
PROSITEiPS51735. VP35_IID. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8JPY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNNKLKVCS GPETTGWISE QLMTGKIPVT DIFIDIDNKP DQMEVRLKPS
60 70 80 90 100
SRSSTRTCTS SSQTEVNYVP LLKKVEDTLT MLVNATSRQN AAIEALENRL
110 120 130 140 150
STLESSLKPI QDMGKVISSL NRSCAEMVAK YDLLVMTTGR ATSTAAAVDA
160 170 180 190 200
YWKEHKQPPP GPALYEENAL KGKIDDPNSY VPDAVQEAYK NLDSTSTLTE
210 220 230 240 250
ENFGKPYISA KDLKEIMYDH LPGFGTAFHQ LVQVICKIGK DNNLLDTIHA
260 270 280 290 300
EFQASLADGD SPQCALIQIT KRVPIFQDVP PPIIHIRSRG DIPRACQKSL
310 320
RPAPPSPKID RGWVCLFKMQ DGKTLGLKI
Length:329
Mass (Da):36,410
Last modified:October 1, 2002 - v1
Checksum:i398BA99B4603C5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522874 Genomic RNA. Translation: AAN04449.1.
AY769362 Genomic RNA. Translation: AAV48575.1.
RefSeqiNP_690581.1. NC_004161.1.

Genome annotation databases

GeneIDi955189.
KEGGivg:955189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522874 Genomic RNA. Translation: AAN04449.1.
AY769362 Genomic RNA. Translation: AAV48575.1.
RefSeqiNP_690581.1. NC_004161.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KS4X-ray2.40A/B160-329[»]
3KS8X-ray2.40A/B/C/D160-329[»]
3L2AX-ray1.71A204-329[»]
4LG2X-ray2.70A/B/C/D205-329[»]
ProteinModelPortaliQ8JPY0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi955189.
KEGGivg:955189.

Miscellaneous databases

EvolutionaryTraceiQ8JPY0.

Family and domain databases

InterProiIPR002953. Filo_VP35.
IPR031163. VP35_IID.
[Graphical view]
PfamiPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
PRINTSiPR01240. FILOVP35.
PROSITEiPS51735. VP35_IID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
    Groseth A., Stroeher U., Theriault S., Feldmann H.
    Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
    Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
    Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Pennsylvania-89.

Entry informationi

Entry nameiVP35_EBORR
AccessioniPrimary (citable) accession number: Q8JPY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.