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Q8JPY0

- VP35_EBORR

UniProt

Q8JPY0 - VP35_EBORR

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Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition host IKBKE and TBK1 producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IKBKE activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host TBK1 activity Source: UniProtKB-KW
  4. suppression by virus of host toll-like receptor signaling pathway Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host IKBKE by virus, Inhibition of host innate immune response by virus, Inhibition of host IRF7 by virus, Inhibition of host RLR pathway by virus, Inhibition of host TBK1 by virus, Inhibition of host TLR pathway by virus, Interferon antiviral system evasion, Transcription, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase cofactor VP35
Gene namesi
Name:VP35
ORF Names:REBOVgp2
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007207: Genome

Subcellular locationi

Virion. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Polymerase cofactor VP35PRO_0000245076Add
BLAST

Post-translational modificationi

Phosphorylated by host IKBKE.By similarity

Interactioni

Subunit structurei

Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein (By similarity). May interact with VP40. Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3 (By similarity).By similarity

Structurei

Secondary structure

1
329
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi210 – 21910
Beta strandi223 – 2253
Helixi227 – 24115
Helixi245 – 25713
Helixi262 – 27211
Helixi275 – 2784
Beta strandi283 – 2853
Helixi289 – 2913
Helixi294 – 2963
Beta strandi299 – 3024
Helixi309 – 3113
Beta strandi313 – 3186
Beta strandi324 – 3285

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KS4X-ray2.40A/B160-329[»]
3KS8X-ray2.40A/B/C/D160-329[»]
3L2AX-ray1.71A204-329[»]
4LG2X-ray2.70A/B/C/D205-329[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8JPY0.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili84 – 11229Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002953. Filo_VP35.
[Graphical view]
PfamiPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
PRINTSiPR01240. FILOVP35.

Sequencei

Sequence statusi: Complete.

Q8JPY0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNNKLKVCS GPETTGWISE QLMTGKIPVT DIFIDIDNKP DQMEVRLKPS
60 70 80 90 100
SRSSTRTCTS SSQTEVNYVP LLKKVEDTLT MLVNATSRQN AAIEALENRL
110 120 130 140 150
STLESSLKPI QDMGKVISSL NRSCAEMVAK YDLLVMTTGR ATSTAAAVDA
160 170 180 190 200
YWKEHKQPPP GPALYEENAL KGKIDDPNSY VPDAVQEAYK NLDSTSTLTE
210 220 230 240 250
ENFGKPYISA KDLKEIMYDH LPGFGTAFHQ LVQVICKIGK DNNLLDTIHA
260 270 280 290 300
EFQASLADGD SPQCALIQIT KRVPIFQDVP PPIIHIRSRG DIPRACQKSL
310 320
RPAPPSPKID RGWVCLFKMQ DGKTLGLKI
Length:329
Mass (Da):36,410
Last modified:October 1, 2002 - v1
Checksum:i398BA99B4603C5CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF522874 Genomic RNA. Translation: AAN04449.1.
AY769362 Genomic RNA. Translation: AAV48575.1.
RefSeqiNP_690581.1. NC_004161.1.

Genome annotation databases

GeneIDi955189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF522874 Genomic RNA. Translation: AAN04449.1 .
AY769362 Genomic RNA. Translation: AAV48575.1 .
RefSeqi NP_690581.1. NC_004161.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3KS4 X-ray 2.40 A/B 160-329 [» ]
3KS8 X-ray 2.40 A/B/C/D 160-329 [» ]
3L2A X-ray 1.71 A 204-329 [» ]
4LG2 X-ray 2.70 A/B/C/D 205-329 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 955189.

Miscellaneous databases

EvolutionaryTracei Q8JPY0.

Family and domain databases

InterProi IPR002953. Filo_VP35.
[Graphical view ]
Pfami PF02097. Filo_VP35. 1 hit.
[Graphical view ]
PIRSFi PIRSF018326. VP35_FiloV. 1 hit.
PRINTSi PR01240. FILOVP35.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
    Groseth A., Stroeher U., Theriault S., Feldmann H.
    Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
    Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
    Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Pennsylvania-89.

Entry informationi

Entry nameiVP35_EBORR
AccessioniPrimary (citable) accession number: Q8JPY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3