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Q8JPY0

- VP35_EBORR

UniProt

Q8JPY0 - VP35_EBORR

Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition host IKBKE and TBK1 producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host IKBKE activity Source: UniProtKB-KW
    2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
    3. suppression by virus of host TBK1 activity Source: UniProtKB-KW
    4. suppression by virus of host toll-like receptor signaling pathway Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host IKBKE by virus, Inhibition of host innate immune response by virus, Inhibition of host IRF7 by virus, Inhibition of host RLR pathway by virus, Inhibition of host TBK1 by virus, Inhibition of host TLR pathway by virus, Interferon antiviral system evasion, Transcription, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase cofactor VP35
    Gene namesi
    Name:VP35
    ORF Names:REBOVgp2
    OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
    Taxonomic identifieri386032 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000007207: Genome

    Subcellular locationi

    Virion. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Polymerase cofactor VP35PRO_0000245076Add
    BLAST

    Post-translational modificationi

    Phosphorylated by host IKBKE.By similarity

    Interactioni

    Subunit structurei

    Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein By similarity. May interact with VP40. Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3 By similarity.By similarity

    Structurei

    Secondary structure

    1
    329
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi210 – 21910
    Beta strandi223 – 2253
    Helixi227 – 24115
    Helixi245 – 25713
    Helixi262 – 27211
    Helixi275 – 2784
    Beta strandi283 – 2853
    Helixi289 – 2913
    Helixi294 – 2963
    Beta strandi299 – 3024
    Helixi309 – 3113
    Beta strandi313 – 3186
    Beta strandi324 – 3285

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3KS4X-ray2.40A/B160-329[»]
    3KS8X-ray2.40A/B/C/D160-329[»]
    3L2AX-ray1.71A204-329[»]
    4LG2X-ray2.70A/B/C/D205-329[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8JPY0.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili84 – 11229Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002953. Filo_VP35.
    [Graphical view]
    PfamiPF02097. Filo_VP35. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
    PRINTSiPR01240. FILOVP35.

    Sequencei

    Sequence statusi: Complete.

    Q8JPY0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNNKLKVCS GPETTGWISE QLMTGKIPVT DIFIDIDNKP DQMEVRLKPS    50
    SRSSTRTCTS SSQTEVNYVP LLKKVEDTLT MLVNATSRQN AAIEALENRL 100
    STLESSLKPI QDMGKVISSL NRSCAEMVAK YDLLVMTTGR ATSTAAAVDA 150
    YWKEHKQPPP GPALYEENAL KGKIDDPNSY VPDAVQEAYK NLDSTSTLTE 200
    ENFGKPYISA KDLKEIMYDH LPGFGTAFHQ LVQVICKIGK DNNLLDTIHA 250
    EFQASLADGD SPQCALIQIT KRVPIFQDVP PPIIHIRSRG DIPRACQKSL 300
    RPAPPSPKID RGWVCLFKMQ DGKTLGLKI 329
    Length:329
    Mass (Da):36,410
    Last modified:October 1, 2002 - v1
    Checksum:i398BA99B4603C5CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF522874 Genomic RNA. Translation: AAN04449.1.
    AY769362 Genomic RNA. Translation: AAV48575.1.
    RefSeqiNP_690581.1. NC_004161.1.

    Genome annotation databases

    GeneIDi955189.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF522874 Genomic RNA. Translation: AAN04449.1 .
    AY769362 Genomic RNA. Translation: AAV48575.1 .
    RefSeqi NP_690581.1. NC_004161.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3KS4 X-ray 2.40 A/B 160-329 [» ]
    3KS8 X-ray 2.40 A/B/C/D 160-329 [» ]
    3L2A X-ray 1.71 A 204-329 [» ]
    4LG2 X-ray 2.70 A/B/C/D 205-329 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 955189.

    Miscellaneous databases

    EvolutionaryTracei Q8JPY0.

    Family and domain databases

    InterProi IPR002953. Filo_VP35.
    [Graphical view ]
    Pfami PF02097. Filo_VP35. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018326. VP35_FiloV. 1 hit.
    PRINTSi PR01240. FILOVP35.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
      Groseth A., Stroeher U., Theriault S., Feldmann H.
      Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
      Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
      Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Pennsylvania-89.

    Entry informationi

    Entry nameiVP35_EBORR
    AccessioniPrimary (citable) accession number: Q8JPY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3