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Q8JPX9

- VP40_EBORR

UniProt

Q8JPX9 - VP40_EBORR

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Protein
Matrix protein VP40
Gene
VP40
Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral RNA replication, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein VP40
Alternative name(s):
Membrane-associated protein VP40
Gene namesi
Name:VP40
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007207: Genome

Subcellular locationi

Virion membrane; Peripheral membrane protein By similarity. Host endomembrane system; Peripheral membrane protein By similarity. Host cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity
Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place By similarity.

GO - Cellular componenti

  1. host cell endomembrane system Source: UniProtKB-SubCell
  2. host cell plasma membrane Source: UniProtKB-SubCell
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Matrix protein VP40
PRO_0000245081Add
BLAST

Interactioni

Subunit structurei

Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8JPX9.
SMRiQ8JPX9. Positions 44-321.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2143Important for oligomerization By similarity
Regioni213 – 331119Membrane-binding By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 104PTAP/PSAP motif
Motifi10 – 134PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

Sequence similaritiesi

Family and domain databases

InterProiIPR008986. EV_matrix.
[Graphical view]
PfamiPF07447. VP40. 1 hit.
[Graphical view]
PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
SUPFAMiSSF50012. SSF50012. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8JPX9-1 [UniParc]FASTAAdd to Basket

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MRRGVLPTAP PAYNDIAYPM SILPTRPSVI VNETKSDVLA VPGADVPSNS    50
MRPVADDNID HSSHTPSGVA SAFILEATVN VISGTKVLMK QIPIWLPLGV 100
ADQKIYSFDS TTAAIMLASY TVTHFGKISN PLVRVNRLGP GIPDHPLRLL 150
RLGNQAFLQE FVLPPVQLPQ YFTFDLTALK LITQPLPAAT WTDETPAGAV 200
NALRPGLSLH PKLRPILLPG KTGKKGHASD LTSPDKIQTI MNAIPDLKIV 250
PIDPTKNIVG IEVPELLVQR LTGKKPQPKN GQPIIPVLLP KYVGLDPISP 300
GDLTMVITQD CDSCHSPASH PYHMDKQNSY Q 331
Length:331
Mass (Da):35,821
Last modified:October 1, 2002 - v1
Checksum:i921510B11D204799
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti147 – 1471L → F in strain: Isolate Pennsylvania-89.
Natural varianti154 – 1552NQ → KK in strain: Isolate Pennsylvania-89.
Natural varianti159 – 1602QE → PG in strain: Isolate Pennsylvania-89.
Natural varianti321 – 3211P → T in strain: Isolate Pennsylvania-89.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF522874 Genomic RNA. Translation: AAN04450.1.
AY769362 Genomic RNA. Translation: AAV48576.1.
RefSeqiNP_690582.1. NC_004161.1.

Genome annotation databases

GeneIDi955192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF522874 Genomic RNA. Translation: AAN04450.1 .
AY769362 Genomic RNA. Translation: AAV48576.1 .
RefSeqi NP_690582.1. NC_004161.1.

3D structure databases

ProteinModelPortali Q8JPX9.
SMRi Q8JPX9. Positions 44-321.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 955192.

Family and domain databases

InterProi IPR008986. EV_matrix.
[Graphical view ]
Pfami PF07447. VP40. 1 hit.
[Graphical view ]
PIRSFi PIRSF018327. VP40_FiloV. 1 hit.
SUPFAMi SSF50012. SSF50012. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
    Groseth A., Stroeher U., Theriault S., Feldmann H.
    Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
    Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
    Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Pennsylvania-89.

Entry informationi

Entry nameiVP40_EBORR
AccessioniPrimary (citable) accession number: Q8JPX9
Secondary accession number(s): Q5UAK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant protein in the virion By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi