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Q8JPX6 (VP30_EBORR) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene names
Name:VP30
OrganismReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) [Complete proteome]
Taxonomic identifier386032 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA By similarity.

Subunit structure

Homohexamer or homodimer. Interacts with the nucleoprotein By similarity.

Subcellular location

Virion. Host cytoplasm By similarity.

Post-translational modification

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation By similarity.

Sequence similarities

Belongs to the filoviridae minor nucleoprotein VP30 family.

Contains 1 C3H1-type zinc finger.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentHost cytoplasm
Virion
   DomainZinc-finger
   LigandMetal-binding
RNA-binding
Viral nucleoprotein
Zinc
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

viral nucleocapsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Minor nucleoprotein VP30
PRO_0000245072

Regions

Zinc finger72 – 9019C3H1-type; atypical
Region1 – 4444Intrinsically disordered Potential
Region26 – 4015RNA-binding By similarity
Region94 – 11219Oligomerization By similarity
Region121 – 14020Intrinsically disordered Potential
Region180 – 19718Interaction with the nucleoprotein By similarity
Region268 – 28720Intrinsically disordered Potential

Natural variations

Natural variant671A → T in strain: Isolate Pennsylvania-89.
Natural variant1361Q → H in strain: Isolate Pennsylvania-89.

Secondary structure

.................. 287
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8JPX6 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: FC76BDA604A769CE

FASTA28732,401
        10         20         30         40         50         60 
MEHSRERGRS SNMRHNSREP YENPSRSRSL SRDPNQVDRR QPRSASQIRV PNLFHRKKTD 

        70         80         90        100        110        120 
ALIVPPAPKD ICPTLKKGFL CDSKFCKKDH QLDSLNDHEL LLLIARRTCG IIESNSQITS 

       130        140        150        160        170        180 
PKDMRLANPT AEDFSQGNSP KLTLAVLLQI AEHWATRDLR QIEDSKLRAL LTLCAVLTRK 

       190        200        210        220        230        240 
FSKSQLGLLC ETHLRHEGLG QDQADSVLEV YQRLHSDKGG NFEAALWQQW DRQSLIMFIS 

       250        260        270        280 
AFLNIALQIP CESSSVVVSG LATLYPAQDN STPSEATNDT TWSSTVE 

« Hide

References

[1]"Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
Groseth A., Stroeher U., Theriault S., Feldmann H.
Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Pennsylvania-89.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF522874 Genomic RNA. Translation: AAN04452.1.
AY769362 Genomic RNA. Translation: AAV48579.1.
RefSeqNP_690585.1. NC_004161.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3V7OX-ray2.25A/B142-272[»]
ProteinModelPortalQ8JPX6.
SMRQ8JPX6. Positions 142-266.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID955196.

Family and domain databases

InterProIPR014459. VP30_FiloV.
[Graphical view]
PfamPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVP30_EBORR
AccessionPrimary (citable) accession number: Q8JPX6
Secondary accession number(s): Q5UAK6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: March 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references