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Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 90C3H1-type; atypicalAdd BLAST19

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000007207 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002450721 – 287Minor nucleoprotein VP30Add BLAST287

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein (By similarity).By similarity

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi144 – 155Combined sources12
Helixi164 – 178Combined sources15
Helixi183 – 185Combined sources3
Helixi186 – 197Combined sources12
Helixi201 – 203Combined sources3
Helixi204 – 215Combined sources12
Helixi221 – 229Combined sources9
Helixi232 – 245Combined sources14
Helixi246 – 248Combined sources3
Helixi255 – 262Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V7OX-ray2.25A/B142-272[»]
ProteinModelPortaliQ8JPX6.
SMRiQ8JPX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 44Intrinsically disorderedSequence analysisAdd BLAST44
Regioni26 – 40RNA-bindingBy similarityAdd BLAST15
Regioni94 – 112OligomerizationBy similarityAdd BLAST19
Regioni121 – 140Intrinsically disorderedSequence analysisAdd BLAST20
Regioni180 – 197Interaction with the nucleoproteinBy similarityAdd BLAST18
Regioni268 – 287Intrinsically disorderedSequence analysisAdd BLAST20

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 90C3H1-type; atypicalAdd BLAST19

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8JPX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHSRERGRS SNMRHNSREP YENPSRSRSL SRDPNQVDRR QPRSASQIRV
60 70 80 90 100
PNLFHRKKTD ALIVPPAPKD ICPTLKKGFL CDSKFCKKDH QLDSLNDHEL
110 120 130 140 150
LLLIARRTCG IIESNSQITS PKDMRLANPT AEDFSQGNSP KLTLAVLLQI
160 170 180 190 200
AEHWATRDLR QIEDSKLRAL LTLCAVLTRK FSKSQLGLLC ETHLRHEGLG
210 220 230 240 250
QDQADSVLEV YQRLHSDKGG NFEAALWQQW DRQSLIMFIS AFLNIALQIP
260 270 280
CESSSVVVSG LATLYPAQDN STPSEATNDT TWSSTVE
Length:287
Mass (Da):32,401
Last modified:October 1, 2002 - v1
Checksum:iFC76BDA604A769CE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti67A → T in strain: Isolate Pennsylvania-89. 1
Natural varianti136Q → H in strain: Isolate Pennsylvania-89. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522874 Genomic RNA. Translation: AAN04452.1.
AY769362 Genomic RNA. Translation: AAV48579.1.
RefSeqiNP_690585.1. NC_004161.1.

Genome annotation databases

GeneIDi955196.
KEGGivg:955196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522874 Genomic RNA. Translation: AAN04452.1.
AY769362 Genomic RNA. Translation: AAV48579.1.
RefSeqiNP_690585.1. NC_004161.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V7OX-ray2.25A/B142-272[»]
ProteinModelPortaliQ8JPX6.
SMRiQ8JPX6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi955196.
KEGGivg:955196.

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVP30_EBORR
AccessioniPrimary (citable) accession number: Q8JPX6
Secondary accession number(s): Q5UAK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.