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Q8JPX6

- VP30_EBORR

UniProt

Q8JPX6 - VP30_EBORR

Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 9019C3H1-type; atypicalAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Minor nucleoprotein VP30
    Alternative name(s):
    Transcription activator VP30
    Gene namesi
    Name:VP30
    OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
    Taxonomic identifieri386032 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000007207: Genome

    Subcellular locationi

    Virion. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 287287Minor nucleoprotein VP30PRO_0000245072Add
    BLAST

    Post-translational modificationi

    Phosphorylated by host. Phosphorylation negatively regulates the transcription activation By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homohexamer or homodimer. Interacts with the nucleoprotein By similarity.By similarity

    Structurei

    Secondary structure

    1
    287
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi144 – 15512
    Helixi164 – 17815
    Helixi183 – 1853
    Helixi186 – 19712
    Helixi201 – 2033
    Helixi204 – 21512
    Helixi221 – 2299
    Helixi232 – 24514
    Helixi246 – 2483
    Helixi255 – 2628

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3V7OX-ray2.25A/B142-272[»]
    ProteinModelPortaliQ8JPX6.
    SMRiQ8JPX6. Positions 142-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4444Intrinsically disorderedSequence AnalysisAdd
    BLAST
    Regioni26 – 4015RNA-bindingBy similarityAdd
    BLAST
    Regioni94 – 11219OligomerizationBy similarityAdd
    BLAST
    Regioni121 – 14020Intrinsically disorderedSequence AnalysisAdd
    BLAST
    Regioni180 – 19718Interaction with the nucleoproteinBy similarityAdd
    BLAST
    Regioni268 – 28720Intrinsically disorderedSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C3H1-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 9019C3H1-type; atypicalAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR014459. VP30_FiloV.
    [Graphical view]
    PfamiPF11507. Transcript_VP30. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8JPX6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEHSRERGRS SNMRHNSREP YENPSRSRSL SRDPNQVDRR QPRSASQIRV    50
    PNLFHRKKTD ALIVPPAPKD ICPTLKKGFL CDSKFCKKDH QLDSLNDHEL 100
    LLLIARRTCG IIESNSQITS PKDMRLANPT AEDFSQGNSP KLTLAVLLQI 150
    AEHWATRDLR QIEDSKLRAL LTLCAVLTRK FSKSQLGLLC ETHLRHEGLG 200
    QDQADSVLEV YQRLHSDKGG NFEAALWQQW DRQSLIMFIS AFLNIALQIP 250
    CESSSVVVSG LATLYPAQDN STPSEATNDT TWSSTVE 287
    Length:287
    Mass (Da):32,401
    Last modified:October 1, 2002 - v1
    Checksum:iFC76BDA604A769CE
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti67 – 671A → T in strain: Isolate Pennsylvania-89.
    Natural varianti136 – 1361Q → H in strain: Isolate Pennsylvania-89.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF522874 Genomic RNA. Translation: AAN04452.1.
    AY769362 Genomic RNA. Translation: AAV48579.1.
    RefSeqiNP_690585.1. NC_004161.1.

    Genome annotation databases

    GeneIDi955196.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF522874 Genomic RNA. Translation: AAN04452.1 .
    AY769362 Genomic RNA. Translation: AAV48579.1 .
    RefSeqi NP_690585.1. NC_004161.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3V7O X-ray 2.25 A/B 142-272 [» ]
    ProteinModelPortali Q8JPX6.
    SMRi Q8JPX6. Positions 142-266.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 955196.

    Family and domain databases

    InterProi IPR014459. VP30_FiloV.
    [Graphical view ]
    Pfami PF11507. Transcript_VP30. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011356. VP30_FiloV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
      Groseth A., Stroeher U., Theriault S., Feldmann H.
      Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
      Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
      Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Pennsylvania-89.

    Entry informationi

    Entry nameiVP30_EBORR
    AccessioniPrimary (citable) accession number: Q8JPX6
    Secondary accession number(s): Q5UAK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3