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Q8JPX6

- VP30_EBORR

UniProt

Q8JPX6 - VP30_EBORR

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Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA By similarity.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007207: Genome

Subcellular locationi

Virion. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Minor nucleoprotein VP30PRO_0000245072Add
BLAST

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation By similarity.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein By similarity.By similarity

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi144 – 15512
Helixi164 – 17815
Helixi183 – 1853
Helixi186 – 19712
Helixi201 – 2033
Helixi204 – 21512
Helixi221 – 2299
Helixi232 – 24514
Helixi246 – 2483
Helixi255 – 2628

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V7OX-ray2.25A/B142-272[»]
ProteinModelPortaliQ8JPX6.
SMRiQ8JPX6. Positions 142-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Intrinsically disorderedSequence AnalysisAdd
BLAST
Regioni26 – 4015RNA-bindingBy similarityAdd
BLAST
Regioni94 – 11219OligomerizationBy similarityAdd
BLAST
Regioni121 – 14020Intrinsically disorderedSequence AnalysisAdd
BLAST
Regioni180 – 19718Interaction with the nucleoproteinBy similarityAdd
BLAST
Regioni268 – 28720Intrinsically disorderedSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8JPX6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEHSRERGRS SNMRHNSREP YENPSRSRSL SRDPNQVDRR QPRSASQIRV
60 70 80 90 100
PNLFHRKKTD ALIVPPAPKD ICPTLKKGFL CDSKFCKKDH QLDSLNDHEL
110 120 130 140 150
LLLIARRTCG IIESNSQITS PKDMRLANPT AEDFSQGNSP KLTLAVLLQI
160 170 180 190 200
AEHWATRDLR QIEDSKLRAL LTLCAVLTRK FSKSQLGLLC ETHLRHEGLG
210 220 230 240 250
QDQADSVLEV YQRLHSDKGG NFEAALWQQW DRQSLIMFIS AFLNIALQIP
260 270 280
CESSSVVVSG LATLYPAQDN STPSEATNDT TWSSTVE
Length:287
Mass (Da):32,401
Last modified:October 1, 2002 - v1
Checksum:iFC76BDA604A769CE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671A → T in strain: Isolate Pennsylvania-89.
Natural varianti136 – 1361Q → H in strain: Isolate Pennsylvania-89.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF522874 Genomic RNA. Translation: AAN04452.1.
AY769362 Genomic RNA. Translation: AAV48579.1.
RefSeqiNP_690585.1. NC_004161.1.

Genome annotation databases

GeneIDi955196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF522874 Genomic RNA. Translation: AAN04452.1 .
AY769362 Genomic RNA. Translation: AAV48579.1 .
RefSeqi NP_690585.1. NC_004161.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3V7O X-ray 2.25 A/B 142-272 [» ]
ProteinModelPortali Q8JPX6.
SMRi Q8JPX6. Positions 142-266.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 955196.

Family and domain databases

InterProi IPR014459. VP30_FiloV.
[Graphical view ]
Pfami PF11507. Transcript_VP30. 1 hit.
[Graphical view ]
PIRSFi PIRSF011356. VP30_FiloV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
    Groseth A., Stroeher U., Theriault S., Feldmann H.
    Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
    Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
    Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Pennsylvania-89.

Entry informationi

Entry nameiVP30_EBORR
AccessioniPrimary (citable) accession number: Q8JPX6
Secondary accession number(s): Q5UAK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3