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Q8JPX5

- L_EBORR

UniProt

Q8JPX5 - L_EBORR

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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007207: Genome

Subcellular locationi

Host cytoplasm. Virion By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22122212RNA-directed RNA polymerase LPRO_0000245048Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 809185RdRp catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini1803 – 2001199Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8JPX5-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRQCKLPKHI
60 70 80 90 100
YRLKFDTIVS KFLSDTPVAT LPIDYLVPIL LRSLTGHGDR PLTPTCNQFL
110 120 130 140 150
DEIINYTLHD AAFLDYYLKA TGAQDHLTNI ATREKLKNEI LNNDYVHQLF
160 170 180 190 200
FWHDLSILAR RGRLNRGNNR STWFVHDEFI DILGYGDYIF WKIPLSLLPV
210 220 230 240 250
TIDGVPHAAT DWYQPTLFKE SILGHSQILS VSTAEILIMC KDIITCRFNT
260 270 280 290 300
SLIASIAKLE DVDVSDYPDP SDILKIYNAG DYVISILGSE GYKIIKYLEP
310 320 330 340 350
LCLAKIQLCS KFTERKGRFL TQMHLSVIND LRELISNRRL KDYQQEKIRD
360 370 380 390 400
FHKILLQLQL SPQQFCELFS VQKHWGHPIL HSEKAIQKVK RHATILKALR
410 420 430 440 450
PNVIFETYCV FKYNIAKHYF DSQGTWYSVI SDRNLTPGLN SFIKRNHFPS
460 470 480 490 500
LPMIKDLLWE FYHLNHPPLF STKVISDLSI FIKDRATAVE QTCWDAVFEP
510 520 530 540 550
NVLGYNPPNK FSTKRVPEQF LEQEDFSIES VLNYAQELHY LLPQNRNFSF
560 570 580 590 600
SLKEKELNIG RTFGKLPYLT RNVQTLCEAL LADGLAKAFP SNMMVVTERE
610 620 630 640 650
QKESLLHQAS WHHTSDDFGE NATVRGSSFV TDLEKYNLAF RYEFTAPFIE
660 670 680 690 700
YCNHCYGVRN VFNWMHYLIP QCYMHVSDYY NPPHNVNLSN REYPPEGPSS
710 720 730 740 750
YRGHLGGIEG LQQKLWTSIS CAQISLVEIK TGFKLRSAVM GDNQCITVLS
760 770 780 790 800
VFPLKTDPEE QEQSAEDNAA RVAASLAKVT SACGIFLKPD ETFVHSGFIY
810 820 830 840 850
FGKKQYLNGV QLPQSLKTAA RMAPLSDAIF DDLQGTLASI GTAFERAISE
860 870 880 890 900
TRHILPCRIV AAFHTYFAVR ILQYHHLGFN KGIDLGQLSL SKPLDYGTIT
910 920 930 940 950
LTLAVPQVLG GLSFLNPEKC FYRNFGDPVT SGLFQLRVYL EMVNMKDLFC
960 970 980 990 1000
PLISKNPGNC SAIDFVLNPS GLNVPGSQDL TSFLRQIVRR SITLTARNKL
1010 1020 1030 1040 1050
INTLFHASAD LEDEMVCKWL LSSNPVMSRF AADIFSRTPS GKRLQILGYL
1060 1070 1080 1090 1100
EGTRTLLASK IINNNSETPV LDKLRKITLQ RWNLWFSYLD HCDQLLADAL
1110 1120 1130 1140 1150
QKISCTVDLA QILREYTWSH ILEGRSLIGA TLPCMVEQFK VKWLGQYEPC
1160 1170 1180 1190 1200
PECLNKKGSN AYVSVAVKDQ VVSAWPNTSR ISWTIGSGVP YIGSRTEDKI
1210 1220 1230 1240 1250
GQPAIKPRCP SSALKEAIEL ASRLTWVTQG GSNSEQLIRP FLEARVNLSV
1260 1270 1280 1290 1300
SEVLQMTPSH YSGNIVHRYN DQYSPHSFMA NRMSNTATRL IVSTNTLGEF
1310 1320 1330 1340 1350
SGGGQAARDS NIIFQNVINL AVALYDIRFR NTNTSDIRHN RAHLHLTECC
1360 1370 1380 1390 1400
TKEVPAQYLT YTSALNLDLS RYRDNELIYD SNPLKGGLNC NLTIDSPLVK
1410 1420 1430 1440 1450
GPRLNMIEDD LLRFPHLSGW ELAKTVVQSI ISDNSNSSTD PISSGETRSF
1460 1470 1480 1490 1500
TTHFLTYPQI GLLYSFGAVL CFYLGNTILW TKKLDYEQFL YYLHNQLHNL
1510 1520 1530 1540 1550
PHRALRVFKP TFKHASVMSR LMEIDSNFSI YIGGTSGDRG LSDAARLFLR
1560 1570 1580 1590 1600
TAIASFLQFL KSWIIDRQKT IPLWIVYPLE GQQPESINEF LHKILGLLKQ
1610 1620 1630 1640 1650
GPKSIPKEVS IQNDGHLDLA ENNYVYNSKS TASNFFHASL AYWRSRKSRK
1660 1670 1680 1690 1700
TQDHNDFSRG DGTLTEPVRK FSSNHQSDEK YYNVTCGKSP KPQERKDFSQ
1710 1720 1730 1740 1750
YRLSNNGQTM SNHRKKGKFH KWNPCKMLME SQRGTVLTEG DYFQNNTPPT
1760 1770 1780 1790 1800
DDVSSPHRLI LPFFKLGNHN HAHDQDAQEL MNQNIKQYLH QLRSMLDTTI
1810 1820 1830 1840 1850
YCRFTGIVSS MHYKLDEVLL EYNSFDSAIT LAEGEGSGAL LLLQKYSTRL
1860 1870 1880 1890 1900
LFLNTLATEH SIESEVVSGF STPRMLLPIM QKVHEGQVTV ILNNSASQIT
1910 1920 1930 1940 1950
DITSSMWLSN QKYNLPCQVE IIMMDAETTE NLNRSQLYRA VYNLILDHID
1960 1970 1980 1990 2000
PQYLKVVVLK VFLSDIEGIL WINDYLAPLF GAGYLIKPIT SSARSSEWYL
2010 2020 2030 2040 2050
CLSNLISTNR RSAHQTHKAC LGVIRDALQA QVQRGVYWLS HIAQYATKNL
2060 2070 2080 2090 2100
HCEYIGLGFP SLEKVLYHRY NLVDTGLGPL SSVIRHLTNL QAEIRDLVLD
2110 2120 2130 2140 2150
YNLMRESRTQ TYHFIKTAKG RITKLVNDFL KFSLIVQALK NNSSWYTELK
2160 2170 2180 2190 2200
KLPEVINVCN RFYHTHNCEC QEKFFVQTLY LQRLRDAEIK LIERLTGLMR
2210
FYPEGLIYSN HT
Length:2,212
Mass (Da):252,549
Last modified:October 1, 2002 - v1
Checksum:iC28CE580881136E3
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti192 – 1921K → R in strain: Isolate Pennsylvania-89.
Natural varianti324 – 3241H → R in strain: Isolate Pennsylvania-89.
Natural varianti442 – 4421F → V in strain: Isolate Pennsylvania-89.
Natural varianti600 – 6001E → G in strain: Isolate Pennsylvania-89.
Natural varianti755 – 7551K → E in strain: Isolate Pennsylvania-89.
Natural varianti1374 – 13741D → G in strain: Isolate Pennsylvania-89.
Natural varianti1487 – 14871E → D in strain: Isolate Pennsylvania-89.
Natural varianti1679 – 16791E → A in strain: Isolate Pennsylvania-89.
Natural varianti1896 – 18983ASQ → SSH in strain: Isolate Pennsylvania-89.
Natural varianti1909 – 19091S → SS in strain: Isolate Pennsylvania-89.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522874 Genomic RNA. Translation: AAN04454.1.
AY769362 Genomic RNA. Translation: AAV48581.1.
RefSeqiNP_690587.1. NC_004161.1.

Genome annotation databases

GeneIDi955191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522874 Genomic RNA. Translation: AAN04454.1 .
AY769362 Genomic RNA. Translation: AAV48581.1 .
RefSeqi NP_690587.1. NC_004161.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 955191.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
    Groseth A., Stroeher U., Theriault S., Feldmann H.
    Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
    Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
    Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Pennsylvania-89.

Entry informationi

Entry nameiL_EBORR
AccessioniPrimary (citable) accession number: Q8JPX5
Secondary accession number(s): Q5UAK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3