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Q8JPX5

- L_EBORR

UniProt

Q8JPX5 - L_EBORR

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 3 domains:
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    mRNA guanylyltransferase (EC:2.7.7.-)
    Gene namesi
    Name:L
    OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
    Taxonomic identifieri386032 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000007207: Genome

    Subcellular locationi

    Host cytoplasm. Virion By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22122212RNA-directed RNA polymerase LPRO_0000245048Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini625 – 809185RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini1803 – 2001199Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view]
    PfamiPF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
    PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8JPX5-1 [UniParc]FASTAAdd to Basket

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    MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRQCKLPKHI     50
    YRLKFDTIVS KFLSDTPVAT LPIDYLVPIL LRSLTGHGDR PLTPTCNQFL 100
    DEIINYTLHD AAFLDYYLKA TGAQDHLTNI ATREKLKNEI LNNDYVHQLF 150
    FWHDLSILAR RGRLNRGNNR STWFVHDEFI DILGYGDYIF WKIPLSLLPV 200
    TIDGVPHAAT DWYQPTLFKE SILGHSQILS VSTAEILIMC KDIITCRFNT 250
    SLIASIAKLE DVDVSDYPDP SDILKIYNAG DYVISILGSE GYKIIKYLEP 300
    LCLAKIQLCS KFTERKGRFL TQMHLSVIND LRELISNRRL KDYQQEKIRD 350
    FHKILLQLQL SPQQFCELFS VQKHWGHPIL HSEKAIQKVK RHATILKALR 400
    PNVIFETYCV FKYNIAKHYF DSQGTWYSVI SDRNLTPGLN SFIKRNHFPS 450
    LPMIKDLLWE FYHLNHPPLF STKVISDLSI FIKDRATAVE QTCWDAVFEP 500
    NVLGYNPPNK FSTKRVPEQF LEQEDFSIES VLNYAQELHY LLPQNRNFSF 550
    SLKEKELNIG RTFGKLPYLT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 600
    QKESLLHQAS WHHTSDDFGE NATVRGSSFV TDLEKYNLAF RYEFTAPFIE 650
    YCNHCYGVRN VFNWMHYLIP QCYMHVSDYY NPPHNVNLSN REYPPEGPSS 700
    YRGHLGGIEG LQQKLWTSIS CAQISLVEIK TGFKLRSAVM GDNQCITVLS 750
    VFPLKTDPEE QEQSAEDNAA RVAASLAKVT SACGIFLKPD ETFVHSGFIY 800
    FGKKQYLNGV QLPQSLKTAA RMAPLSDAIF DDLQGTLASI GTAFERAISE 850
    TRHILPCRIV AAFHTYFAVR ILQYHHLGFN KGIDLGQLSL SKPLDYGTIT 900
    LTLAVPQVLG GLSFLNPEKC FYRNFGDPVT SGLFQLRVYL EMVNMKDLFC 950
    PLISKNPGNC SAIDFVLNPS GLNVPGSQDL TSFLRQIVRR SITLTARNKL 1000
    INTLFHASAD LEDEMVCKWL LSSNPVMSRF AADIFSRTPS GKRLQILGYL 1050
    EGTRTLLASK IINNNSETPV LDKLRKITLQ RWNLWFSYLD HCDQLLADAL 1100
    QKISCTVDLA QILREYTWSH ILEGRSLIGA TLPCMVEQFK VKWLGQYEPC 1150
    PECLNKKGSN AYVSVAVKDQ VVSAWPNTSR ISWTIGSGVP YIGSRTEDKI 1200
    GQPAIKPRCP SSALKEAIEL ASRLTWVTQG GSNSEQLIRP FLEARVNLSV 1250
    SEVLQMTPSH YSGNIVHRYN DQYSPHSFMA NRMSNTATRL IVSTNTLGEF 1300
    SGGGQAARDS NIIFQNVINL AVALYDIRFR NTNTSDIRHN RAHLHLTECC 1350
    TKEVPAQYLT YTSALNLDLS RYRDNELIYD SNPLKGGLNC NLTIDSPLVK 1400
    GPRLNMIEDD LLRFPHLSGW ELAKTVVQSI ISDNSNSSTD PISSGETRSF 1450
    TTHFLTYPQI GLLYSFGAVL CFYLGNTILW TKKLDYEQFL YYLHNQLHNL 1500
    PHRALRVFKP TFKHASVMSR LMEIDSNFSI YIGGTSGDRG LSDAARLFLR 1550
    TAIASFLQFL KSWIIDRQKT IPLWIVYPLE GQQPESINEF LHKILGLLKQ 1600
    GPKSIPKEVS IQNDGHLDLA ENNYVYNSKS TASNFFHASL AYWRSRKSRK 1650
    TQDHNDFSRG DGTLTEPVRK FSSNHQSDEK YYNVTCGKSP KPQERKDFSQ 1700
    YRLSNNGQTM SNHRKKGKFH KWNPCKMLME SQRGTVLTEG DYFQNNTPPT 1750
    DDVSSPHRLI LPFFKLGNHN HAHDQDAQEL MNQNIKQYLH QLRSMLDTTI 1800
    YCRFTGIVSS MHYKLDEVLL EYNSFDSAIT LAEGEGSGAL LLLQKYSTRL 1850
    LFLNTLATEH SIESEVVSGF STPRMLLPIM QKVHEGQVTV ILNNSASQIT 1900
    DITSSMWLSN QKYNLPCQVE IIMMDAETTE NLNRSQLYRA VYNLILDHID 1950
    PQYLKVVVLK VFLSDIEGIL WINDYLAPLF GAGYLIKPIT SSARSSEWYL 2000
    CLSNLISTNR RSAHQTHKAC LGVIRDALQA QVQRGVYWLS HIAQYATKNL 2050
    HCEYIGLGFP SLEKVLYHRY NLVDTGLGPL SSVIRHLTNL QAEIRDLVLD 2100
    YNLMRESRTQ TYHFIKTAKG RITKLVNDFL KFSLIVQALK NNSSWYTELK 2150
    KLPEVINVCN RFYHTHNCEC QEKFFVQTLY LQRLRDAEIK LIERLTGLMR 2200
    FYPEGLIYSN HT 2212
    Length:2,212
    Mass (Da):252,549
    Last modified:October 1, 2002 - v1
    Checksum:iC28CE580881136E3
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti192 – 1921K → R in strain: Isolate Pennsylvania-89.
    Natural varianti324 – 3241H → R in strain: Isolate Pennsylvania-89.
    Natural varianti442 – 4421F → V in strain: Isolate Pennsylvania-89.
    Natural varianti600 – 6001E → G in strain: Isolate Pennsylvania-89.
    Natural varianti755 – 7551K → E in strain: Isolate Pennsylvania-89.
    Natural varianti1374 – 13741D → G in strain: Isolate Pennsylvania-89.
    Natural varianti1487 – 14871E → D in strain: Isolate Pennsylvania-89.
    Natural varianti1679 – 16791E → A in strain: Isolate Pennsylvania-89.
    Natural varianti1896 – 18983ASQ → SSH in strain: Isolate Pennsylvania-89.
    Natural varianti1909 – 19091S → SS in strain: Isolate Pennsylvania-89.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF522874 Genomic RNA. Translation: AAN04454.1.
    AY769362 Genomic RNA. Translation: AAV48581.1.
    RefSeqiNP_690587.1. NC_004161.1.

    Genome annotation databases

    GeneIDi955191.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF522874 Genomic RNA. Translation: AAN04454.1 .
    AY769362 Genomic RNA. Translation: AAV48581.1 .
    RefSeqi NP_690587.1. NC_004161.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 955191.

    Family and domain databases

    InterProi IPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view ]
    Pfami PF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
    PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
      Groseth A., Stroeher U., Theriault S., Feldmann H.
      Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
      Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
      Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Pennsylvania-89.

    Entry informationi

    Entry nameiL_EBORR
    AccessioniPrimary (citable) accession number: Q8JPX5
    Secondary accession number(s): Q5UAK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3