Q8JPX5 (L_EBORR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) [Complete proteome]

Taxonomic identifier

386032 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Ebolavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Epomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length	2212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Host cytoplasm. Virion By similarity.
Sequence similarities	Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2212	2212	RNA-directed RNA polymerase L		PRO_0000245048
Regions
	Domain	625 – 809	185	RdRp catalytic
Natural variations
	Natural variant	192	1	K → R in strain: Isolate Pennsylvania-89.
	Natural variant	324	1	H → R in strain: Isolate Pennsylvania-89.
	Natural variant	442	1	F → V in strain: Isolate Pennsylvania-89.
	Natural variant	600	1	E → G in strain: Isolate Pennsylvania-89.
	Natural variant	755	1	K → E in strain: Isolate Pennsylvania-89.
	Natural variant	1374	1	D → G in strain: Isolate Pennsylvania-89.
	Natural variant	1487	1	E → D in strain: Isolate Pennsylvania-89.
	Natural variant	1679	1	E → A in strain: Isolate Pennsylvania-89.
	Natural variant	1896 – 1898	3	ASQ → SSH in strain: Isolate Pennsylvania-89.
	Natural variant	1909	1	S → SS in strain: Isolate Pennsylvania-89.

Sequences

	Sequence		Length	Mass (Da)
	Q8JPX5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C28CE580881136E3	FASTA	2,212	252,549
	10 20 30 40 50 60 MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRQCKLPKHI YRLKFDTIVS 70 80 90 100 110 120 KFLSDTPVAT LPIDYLVPIL LRSLTGHGDR PLTPTCNQFL DEIINYTLHD AAFLDYYLKA 130 140 150 160 170 180 TGAQDHLTNI ATREKLKNEI LNNDYVHQLF FWHDLSILAR RGRLNRGNNR STWFVHDEFI 190 200 210 220 230 240 DILGYGDYIF WKIPLSLLPV TIDGVPHAAT DWYQPTLFKE SILGHSQILS VSTAEILIMC 250 260 270 280 290 300 KDIITCRFNT SLIASIAKLE DVDVSDYPDP SDILKIYNAG DYVISILGSE GYKIIKYLEP 310 320 330 340 350 360 LCLAKIQLCS KFTERKGRFL TQMHLSVIND LRELISNRRL KDYQQEKIRD FHKILLQLQL 370 380 390 400 410 420 SPQQFCELFS VQKHWGHPIL HSEKAIQKVK RHATILKALR PNVIFETYCV FKYNIAKHYF 430 440 450 460 470 480 DSQGTWYSVI SDRNLTPGLN SFIKRNHFPS LPMIKDLLWE FYHLNHPPLF STKVISDLSI 490 500 510 520 530 540 FIKDRATAVE QTCWDAVFEP NVLGYNPPNK FSTKRVPEQF LEQEDFSIES VLNYAQELHY 550 560 570 580 590 600 LLPQNRNFSF SLKEKELNIG RTFGKLPYLT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 610 620 630 640 650 660 QKESLLHQAS WHHTSDDFGE NATVRGSSFV TDLEKYNLAF RYEFTAPFIE YCNHCYGVRN 670 680 690 700 710 720 VFNWMHYLIP QCYMHVSDYY NPPHNVNLSN REYPPEGPSS YRGHLGGIEG LQQKLWTSIS 730 740 750 760 770 780 CAQISLVEIK TGFKLRSAVM GDNQCITVLS VFPLKTDPEE QEQSAEDNAA RVAASLAKVT 790 800 810 820 830 840 SACGIFLKPD ETFVHSGFIY FGKKQYLNGV QLPQSLKTAA RMAPLSDAIF DDLQGTLASI 850 860 870 880 890 900 GTAFERAISE TRHILPCRIV AAFHTYFAVR ILQYHHLGFN KGIDLGQLSL SKPLDYGTIT 910 920 930 940 950 960 LTLAVPQVLG GLSFLNPEKC FYRNFGDPVT SGLFQLRVYL EMVNMKDLFC PLISKNPGNC 970 980 990 1000 1010 1020 SAIDFVLNPS GLNVPGSQDL TSFLRQIVRR SITLTARNKL INTLFHASAD LEDEMVCKWL 1030 1040 1050 1060 1070 1080 LSSNPVMSRF AADIFSRTPS GKRLQILGYL EGTRTLLASK IINNNSETPV LDKLRKITLQ 1090 1100 1110 1120 1130 1140 RWNLWFSYLD HCDQLLADAL QKISCTVDLA QILREYTWSH ILEGRSLIGA TLPCMVEQFK 1150 1160 1170 1180 1190 1200 VKWLGQYEPC PECLNKKGSN AYVSVAVKDQ VVSAWPNTSR ISWTIGSGVP YIGSRTEDKI 1210 1220 1230 1240 1250 1260 GQPAIKPRCP SSALKEAIEL ASRLTWVTQG GSNSEQLIRP FLEARVNLSV SEVLQMTPSH 1270 1280 1290 1300 1310 1320 YSGNIVHRYN DQYSPHSFMA NRMSNTATRL IVSTNTLGEF SGGGQAARDS NIIFQNVINL 1330 1340 1350 1360 1370 1380 AVALYDIRFR NTNTSDIRHN RAHLHLTECC TKEVPAQYLT YTSALNLDLS RYRDNELIYD 1390 1400 1410 1420 1430 1440 SNPLKGGLNC NLTIDSPLVK GPRLNMIEDD LLRFPHLSGW ELAKTVVQSI ISDNSNSSTD 1450 1460 1470 1480 1490 1500 PISSGETRSF TTHFLTYPQI GLLYSFGAVL CFYLGNTILW TKKLDYEQFL YYLHNQLHNL 1510 1520 1530 1540 1550 1560 PHRALRVFKP TFKHASVMSR LMEIDSNFSI YIGGTSGDRG LSDAARLFLR TAIASFLQFL 1570 1580 1590 1600 1610 1620 KSWIIDRQKT IPLWIVYPLE GQQPESINEF LHKILGLLKQ GPKSIPKEVS IQNDGHLDLA 1630 1640 1650 1660 1670 1680 ENNYVYNSKS TASNFFHASL AYWRSRKSRK TQDHNDFSRG DGTLTEPVRK FSSNHQSDEK 1690 1700 1710 1720 1730 1740 YYNVTCGKSP KPQERKDFSQ YRLSNNGQTM SNHRKKGKFH KWNPCKMLME SQRGTVLTEG 1750 1760 1770 1780 1790 1800 DYFQNNTPPT DDVSSPHRLI LPFFKLGNHN HAHDQDAQEL MNQNIKQYLH QLRSMLDTTI 1810 1820 1830 1840 1850 1860 YCRFTGIVSS MHYKLDEVLL EYNSFDSAIT LAEGEGSGAL LLLQKYSTRL LFLNTLATEH 1870 1880 1890 1900 1910 1920 SIESEVVSGF STPRMLLPIM QKVHEGQVTV ILNNSASQIT DITSSMWLSN QKYNLPCQVE 1930 1940 1950 1960 1970 1980 IIMMDAETTE NLNRSQLYRA VYNLILDHID PQYLKVVVLK VFLSDIEGIL WINDYLAPLF 1990 2000 2010 2020 2030 2040 GAGYLIKPIT SSARSSEWYL CLSNLISTNR RSAHQTHKAC LGVIRDALQA QVQRGVYWLS 2050 2060 2070 2080 2090 2100 HIAQYATKNL HCEYIGLGFP SLEKVLYHRY NLVDTGLGPL SSVIRHLTNL QAEIRDLVLD 2110 2120 2130 2140 2150 2160 YNLMRESRTQ TYHFIKTAKG RITKLVNDFL KFSLIVQALK NNSSWYTELK KLPEVINVCN 2170 2180 2190 2200 2210 RFYHTHNCEC QEKFFVQTLY LQRLRDAEIK LIERLTGLMR FYPEGLIYSN HT

References

[1]	"Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States." Groseth A., Stroeher U., Theriault S., Feldmann H. Virus Res. 87:155-163(2002) [PubMed: 12191779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire." Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I. Virology 332:406-417(2005) [PubMed: 15661171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Pennsylvania-89.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF522874 Genomic RNA. Translation: AAN04454.1. AY769362 Genomic RNA. Translation: AAV48581.1.
RefSeq	NP_690587.1. NC_004161.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	955191.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017235. RNA-dir_pol_filovirus. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037548. RNA_pol_Filoviridae. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_EBORR

Accession

Primary (citable) accession number: Q8JPX5
Secondary accession number(s): Q5UAK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program