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Q8JPR2 (L_BUNL8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase L

Short name=Protein L
EC=2.7.7.48
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Gene names
Name:L
OrganismBunyavirus La Crosse (isolate Human/United States/L78/1978) [Reference proteome]
Taxonomic identifier796210 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]

Protein attributes

Sequence length2263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are not polyadenylated. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity.

Sequence similarities

Belongs to the bunyaviridae RNA polymerase family.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 22632263RNA-directed RNA polymerase L
PRO_0000397189

Regions

Domain1042 – 1230189RdRp catalytic
Coiled coil1003 – 103331 Potential

Sequences

Sequence LengthMass (Da)Tools
Q8JPR2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: A612682C8495E627

FASTA2,263263,013
        10         20         30         40         50         60 
MDYQEYQQFL ARINTARDAC VAKDIDVDLL MARHDYFGRE LCKSLNIEYR NDVPFVDIIL 

        70         80         90        100        110        120 
DIRPEVDPLT IDAPHITPDN YLYINNVLYI IDYKVSVSNE SSVITYDKYY ELTRDISDRL 

       130        140        150        160        170        180 
SIPIEIVIVR IDPVSKDLHI NSDRFKELYP TIVVDINFNQ FFDLKQLLYE KFGDDEEFLL 

       190        200        210        220        230        240 
KVAHGDFTLT APWCKTGCPE FWKHPIYKEF KMSMPVPERR LFEESVKFNA YESERWNTNL 

       250        260        270        280        290        300 
VKIREYTKKD YSEHISKSAK NIFLASGFYK QPNKNEISEG WTLMVERVQD QREISKSLHD 

       310        320        330        340        350        360 
QKPSIHFIWG AHNPGNSNNA TFKLILLSKS LQSIKGISTY TEAFKSLGKM MDIGDKAIEY 

       370        380        390        400        410        420 
EEFCMSLKSK ARSSWKQIMN KKLEPKQINN ALVLWEQQFM VNNDLIDKSE KLKLFKNFCG 

       430        440        450        460        470        480 
IGKHKQFKNK MLEDLEVSKP KILDFDDANM YLASLTMMEQ SKKILSKSNG LKPDNFILNE 

       490        500        510        520        530        540 
FGSKIKDANK ETYDNMHKIF ETRYWQCISD FSTLMKNILS VSQYNRHNTF RIAMCANNNV 

       550        560        570        580        590        600 
FAIVFPSADI KTKKATVVYS IIVLHKEEEN IFNPGCLHGT FKCMNGYISI SRAIRLDKER 

       610        620        630        640        650        660 
CQRIVSSPGL FLTTCLLFKH DNPTLVMSDI MNFSIYTSLS ITKSVLSLTE PARYMIMNSL 

       670        680        690        700        710        720 
AISSNVKDYI AEKFSPYTKT LFSVYMTRLI KNACFDAYDQ RQRVQLRDIY LSDYDITQKG 

       730        740        750        760        770        780 
IKDNRELTSI WFPGSVTLKE YLTQIYLPFY FNAKGLHEKH HVMVDLAKTI LEIECEQREN 

       790        800        810        820        830        840 
IKEIWSTNCT KQTVNLKILI HSLCKNLLAD TSRHNHLRNR IENRNNFRRS ITTISTFTSS 

       850        860        870        880        890        900 
KSCLKIGDFR KEKELQSVKQ KKILEVQSRK MRLANPMFVT DEQVCLEVGH CNYEMLRNAM 

       910        920        930        940        950        960 
PNYTDYISTK VFDRLYELLD KGVLTDKPVI EQIMDMMVDH KKFYFTFFNK GQKTSKDREI 

       970        980        990       1000       1010       1020 
FVGEYEAKMC MYAVERIAKE RCKLNPDEMI SEPGDGKLKV LEQKSEQEIR FLVETTRQKN 

      1030       1040       1050       1060       1070       1080 
REIDEAIEAL AAEGYESNLE KIEKLSLGKA KGLKMEINAD MSKWSAQDVF YKYFWLIALD 

      1090       1100       1110       1120       1130       1140 
PILYPQEKER ILYFMCNYMD KELILPDELL FNLLDQKVAY QNDIIATMTN QLNSNTVLIK 

      1150       1160       1170       1180       1190       1200 
RNWLQGNFNY TSSYVHSCAM SVYKEILKEA ITLLDGSILV NSLVHSDDNQ TSITIVQDKM 

      1210       1220       1230       1240       1250       1260 
ENDKIIDFAM KEFERACLTF GCQANMKKTY VTNCIKEFVS LFNLYGEPFS IYGRFLLTSV 

      1270       1280       1290       1300       1310       1320 
GDCAYIGPYE DLASRISSAQ TAIKHGCPPS LAWVSIAISH WMTSLTYNML PGQSNDPIDY 

      1330       1340       1350       1360       1370       1380 
FPAENRKDIP IELNGVLDAP LSMISTVGLE SGNLYFLIKL LSKYTPVMQK RESVVNQIAE 

      1390       1400       1410       1420       1430       1440 
VKNWKVEDLT DNEIFRLKIL RYLVLDAEMD PSDIMGETSD MRGRSILTPR KFTTAGSLRK 

      1450       1460       1470       1480       1490       1500 
LYSFSKYQDR LSSPGGMVEL FTYLLEKPEL LVTKGEDMKD YMESVIFRYN SKRFKESLSI 

      1510       1520       1530       1540       1550       1560 
QNPAQLFIEQ ILFSHKPIID FSGIRDKYIN LHDSRALEKE PDILGKVTFT EAYRLLMRDL 

      1570       1580       1590       1600       1610       1620 
SSLELTNDDI QVIYSYIILN DPMMITIANT HILSIYGSPQ RRMGMSCSTM PEFRNLKLIH 

      1630       1640       1650       1660       1670       1680 
HSPALVLRAY SKNNPDIQGA DPTEMARDLV HLKEFVENTN LEEKMKVRIA INEAEKGQRD 

      1690       1700       1710       1720       1730       1740 
IVFELKEMTR FYQVCYEYVK STEHKIKVFI LPTKSYTTTD FCSLMQGNLI KDKEWYTVHY 

      1750       1760       1770       1780       1790       1800 
LKQILSGGHK AIMQHNATSE QNIAFECFKL ITHFADSFID SLSRSAFLQL IIDEFSYKDV 

      1810       1820       1830       1840       1850       1860 
KVSKLYDIIK NGYNRTDFIP LLFRTGDLRQ ADLDKYDAMK SHERVTWNDW QTSRHLDMGS 

      1870       1880       1890       1900       1910       1920 
INLTITGYNR SITIIGEDNK LTYAELCLTR KTPENITISG RKLLGARHGL KFENMSKIQT 

      1930       1940       1950       1960       1970       1980 
YPGNYYITYR KKDRHQFVYQ IHSHESITRR NEEHMAIRTR IYNEITPVCV VNVAEVDGDQ 

      1990       2000       2010       2020       2030       2040 
RILIRSLDYL NNDIFSLSRI KVGLDEFATI KKAHFSKMVS FEGPPIKTGL LDLTELMKSQ 

      2050       2060       2070       2080       2090       2100 
DLLNLNYDNI RNSNLISFSK LICCEGSDNI NDGLEFLSDD PMNFTEGEAI HSTPIFNIYY 

      2110       2120       2130       2140       2150       2160 
SKRGERHMTY RNAIKLLIER ETKIFEEAFT FSENGFISPE NLGCLEAVVS LIKLLKTNEW 

      2170       2180       2190       2200       2210       2220 
STVIDKCIHI CLIKNGMDHM YHSFDVPKCF MGNPITRDMN WMMFREFINS LPGTDIPPWN 

      2230       2240       2250       2260 
VMTENFKKKC IALINSKLET QRDFSEFTKL MKKEGGRSNI EFD 

« Hide

References

[1]"Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain."
Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes."
Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S.
Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF528165 Genomic RNA. Translation: AAM94387.1.
EF485035 Viral cRNA. Translation: ABQ12635.1.
RefSeqNP_671968.1. NC_004108.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956554.

Family and domain databases

InterProIPR026382. CapSnatchArena.
IPR029124. L_protein_N.
IPR007099. RNA-dir_pol_NSvirus.
IPR014384. RNA-dir_pol_orthobunyavirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view]
PfamPF04196. Bunya_RdRp. 1 hit.
PF15518. L_protein_N. 1 hit.
[Graphical view]
PIRSFPIRSF000824. L_OrthobunV. 1 hit.
TIGRFAMsTIGR04202. capSnatchArena. 1 hit.
PROSITEPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameL_BUNL8
AccessionPrimary (citable) accession number: Q8JPR2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families