Q8JPR2 (L_BUNL8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Bunyavirus La Crosse (isolate Human/United States/L78/1978) [Reference proteome]

Taxonomic identifier

796210 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Orthobunyavirus › California encephalitis virus › Bunyavirus La Crosse

Virus host

Aedes triseriatus (Eastern treehole mosquito) (Ochlerotatus triseriatus) [TaxID: 7162]
Cervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Tamias [TaxID: 13712]

Protein attributes

Sequence length	2263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are not polyadenylated. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity.
Sequence similarities	Belongs to the bunyaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Domain	Coiled coil
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2263	2263	RNA-directed RNA polymerase L		PRO_0000397189
Regions
	Domain	1042 – 1230	189	RdRp catalytic
	Coiled coil	1003 – 1033	31	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8JPR2 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A612682C8495E627	FASTA	2,263	263,013
	10 20 30 40 50 60 MDYQEYQQFL ARINTARDAC VAKDIDVDLL MARHDYFGRE LCKSLNIEYR NDVPFVDIIL 70 80 90 100 110 120 DIRPEVDPLT IDAPHITPDN YLYINNVLYI IDYKVSVSNE SSVITYDKYY ELTRDISDRL 130 140 150 160 170 180 SIPIEIVIVR IDPVSKDLHI NSDRFKELYP TIVVDINFNQ FFDLKQLLYE KFGDDEEFLL 190 200 210 220 230 240 KVAHGDFTLT APWCKTGCPE FWKHPIYKEF KMSMPVPERR LFEESVKFNA YESERWNTNL 250 260 270 280 290 300 VKIREYTKKD YSEHISKSAK NIFLASGFYK QPNKNEISEG WTLMVERVQD QREISKSLHD 310 320 330 340 350 360 QKPSIHFIWG AHNPGNSNNA TFKLILLSKS LQSIKGISTY TEAFKSLGKM MDIGDKAIEY 370 380 390 400 410 420 EEFCMSLKSK ARSSWKQIMN KKLEPKQINN ALVLWEQQFM VNNDLIDKSE KLKLFKNFCG 430 440 450 460 470 480 IGKHKQFKNK MLEDLEVSKP KILDFDDANM YLASLTMMEQ SKKILSKSNG LKPDNFILNE 490 500 510 520 530 540 FGSKIKDANK ETYDNMHKIF ETRYWQCISD FSTLMKNILS VSQYNRHNTF RIAMCANNNV 550 560 570 580 590 600 FAIVFPSADI KTKKATVVYS IIVLHKEEEN IFNPGCLHGT FKCMNGYISI SRAIRLDKER 610 620 630 640 650 660 CQRIVSSPGL FLTTCLLFKH DNPTLVMSDI MNFSIYTSLS ITKSVLSLTE PARYMIMNSL 670 680 690 700 710 720 AISSNVKDYI AEKFSPYTKT LFSVYMTRLI KNACFDAYDQ RQRVQLRDIY LSDYDITQKG 730 740 750 760 770 780 IKDNRELTSI WFPGSVTLKE YLTQIYLPFY FNAKGLHEKH HVMVDLAKTI LEIECEQREN 790 800 810 820 830 840 IKEIWSTNCT KQTVNLKILI HSLCKNLLAD TSRHNHLRNR IENRNNFRRS ITTISTFTSS 850 860 870 880 890 900 KSCLKIGDFR KEKELQSVKQ KKILEVQSRK MRLANPMFVT DEQVCLEVGH CNYEMLRNAM 910 920 930 940 950 960 PNYTDYISTK VFDRLYELLD KGVLTDKPVI EQIMDMMVDH KKFYFTFFNK GQKTSKDREI 970 980 990 1000 1010 1020 FVGEYEAKMC MYAVERIAKE RCKLNPDEMI SEPGDGKLKV LEQKSEQEIR FLVETTRQKN 1030 1040 1050 1060 1070 1080 REIDEAIEAL AAEGYESNLE KIEKLSLGKA KGLKMEINAD MSKWSAQDVF YKYFWLIALD 1090 1100 1110 1120 1130 1140 PILYPQEKER ILYFMCNYMD KELILPDELL FNLLDQKVAY QNDIIATMTN QLNSNTVLIK 1150 1160 1170 1180 1190 1200 RNWLQGNFNY TSSYVHSCAM SVYKEILKEA ITLLDGSILV NSLVHSDDNQ TSITIVQDKM 1210 1220 1230 1240 1250 1260 ENDKIIDFAM KEFERACLTF GCQANMKKTY VTNCIKEFVS LFNLYGEPFS IYGRFLLTSV 1270 1280 1290 1300 1310 1320 GDCAYIGPYE DLASRISSAQ TAIKHGCPPS LAWVSIAISH WMTSLTYNML PGQSNDPIDY 1330 1340 1350 1360 1370 1380 FPAENRKDIP IELNGVLDAP LSMISTVGLE SGNLYFLIKL LSKYTPVMQK RESVVNQIAE 1390 1400 1410 1420 1430 1440 VKNWKVEDLT DNEIFRLKIL RYLVLDAEMD PSDIMGETSD MRGRSILTPR KFTTAGSLRK 1450 1460 1470 1480 1490 1500 LYSFSKYQDR LSSPGGMVEL FTYLLEKPEL LVTKGEDMKD YMESVIFRYN SKRFKESLSI 1510 1520 1530 1540 1550 1560 QNPAQLFIEQ ILFSHKPIID FSGIRDKYIN LHDSRALEKE PDILGKVTFT EAYRLLMRDL 1570 1580 1590 1600 1610 1620 SSLELTNDDI QVIYSYIILN DPMMITIANT HILSIYGSPQ RRMGMSCSTM PEFRNLKLIH 1630 1640 1650 1660 1670 1680 HSPALVLRAY SKNNPDIQGA DPTEMARDLV HLKEFVENTN LEEKMKVRIA INEAEKGQRD 1690 1700 1710 1720 1730 1740 IVFELKEMTR FYQVCYEYVK STEHKIKVFI LPTKSYTTTD FCSLMQGNLI KDKEWYTVHY 1750 1760 1770 1780 1790 1800 LKQILSGGHK AIMQHNATSE QNIAFECFKL ITHFADSFID SLSRSAFLQL IIDEFSYKDV 1810 1820 1830 1840 1850 1860 KVSKLYDIIK NGYNRTDFIP LLFRTGDLRQ ADLDKYDAMK SHERVTWNDW QTSRHLDMGS 1870 1880 1890 1900 1910 1920 INLTITGYNR SITIIGEDNK LTYAELCLTR KTPENITISG RKLLGARHGL KFENMSKIQT 1930 1940 1950 1960 1970 1980 YPGNYYITYR KKDRHQFVYQ IHSHESITRR NEEHMAIRTR IYNEITPVCV VNVAEVDGDQ 1990 2000 2010 2020 2030 2040 RILIRSLDYL NNDIFSLSRI KVGLDEFATI KKAHFSKMVS FEGPPIKTGL LDLTELMKSQ 2050 2060 2070 2080 2090 2100 DLLNLNYDNI RNSNLISFSK LICCEGSDNI NDGLEFLSDD PMNFTEGEAI HSTPIFNIYY 2110 2120 2130 2140 2150 2160 SKRGERHMTY RNAIKLLIER ETKIFEEAFT FSENGFISPE NLGCLEAVVS LIKLLKTNEW 2170 2180 2190 2200 2210 2220 STVIDKCIHI CLIKNGMDHM YHSFDVPKCF MGNPITRDMN WMMFREFINS LPGTDIPPWN 2230 2240 2250 2260 VMTENFKKKC IALINSKLET QRDFSEFTKL MKKEGGRSNI EFD

References

[1]	"Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain." Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes." Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S. Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF528165 Genomic RNA. Translation: AAM94387.1. EF485035 Viral cRNA. Translation: ABQ12635.1.
RefSeq	NP_671968.1. NC_004108.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956554.
Family and domain databases
InterPro	IPR026382. CapSnatchArena. IPR007099. RNA-dir_pol_NSvirus. IPR014384. RNA-dir_pol_orthobunyavirus. IPR007322. RNA_pol_bunyavir. [Graphical view]
Pfam	PF04196. Bunya_RdRp. 1 hit. [Graphical view]
PIRSF	PIRSF000824. L_OrthobunV. 1 hit.
TIGRFAMs	TIGR04202. capSnatchArena. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_BUNL8

Accession

Primary (citable) accession number: Q8JPR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	October 1, 2002
Last modified:	October 31, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program