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Q8JPR2

- L_BUNL8

UniProt

Q8JPR2 - L_BUNL8

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Bunyavirus La Crosse (isolate Human/United States/L78/1978)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are not polyadenylated. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L (EC:2.7.7.48)
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Gene namesi
    Name:L
    OrganismiBunyavirus La Crosse (isolate Human/United States/L78/1978)
    Taxonomic identifieri796210 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
    Virus hostiCervidae (deer) [TaxID: 9850]
    Homo sapiens (Human) [TaxID: 9606]
    Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
    Tamias [TaxID: 13712]
    ProteomesiUP000008768: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22632263RNA-directed RNA polymerase LPRO_0000397189Add
    BLAST

    Interactioni

    Subunit structurei

    Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1042 – 1230189RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1003 – 103331Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bunyaviridae RNA polymerase family.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR026382. CapSnatchArena.
    IPR029124. L_protein_N.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR014384. RNA-dir_pol_orthobunyavirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view]
    PfamiPF04196. Bunya_RdRp. 1 hit.
    PF15518. L_protein_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000824. L_OrthobunV. 1 hit.
    TIGRFAMsiTIGR04202. capSnatchArena. 1 hit.
    PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8JPR2-1 [UniParc]FASTAAdd to Basket

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    MDYQEYQQFL ARINTARDAC VAKDIDVDLL MARHDYFGRE LCKSLNIEYR     50
    NDVPFVDIIL DIRPEVDPLT IDAPHITPDN YLYINNVLYI IDYKVSVSNE 100
    SSVITYDKYY ELTRDISDRL SIPIEIVIVR IDPVSKDLHI NSDRFKELYP 150
    TIVVDINFNQ FFDLKQLLYE KFGDDEEFLL KVAHGDFTLT APWCKTGCPE 200
    FWKHPIYKEF KMSMPVPERR LFEESVKFNA YESERWNTNL VKIREYTKKD 250
    YSEHISKSAK NIFLASGFYK QPNKNEISEG WTLMVERVQD QREISKSLHD 300
    QKPSIHFIWG AHNPGNSNNA TFKLILLSKS LQSIKGISTY TEAFKSLGKM 350
    MDIGDKAIEY EEFCMSLKSK ARSSWKQIMN KKLEPKQINN ALVLWEQQFM 400
    VNNDLIDKSE KLKLFKNFCG IGKHKQFKNK MLEDLEVSKP KILDFDDANM 450
    YLASLTMMEQ SKKILSKSNG LKPDNFILNE FGSKIKDANK ETYDNMHKIF 500
    ETRYWQCISD FSTLMKNILS VSQYNRHNTF RIAMCANNNV FAIVFPSADI 550
    KTKKATVVYS IIVLHKEEEN IFNPGCLHGT FKCMNGYISI SRAIRLDKER 600
    CQRIVSSPGL FLTTCLLFKH DNPTLVMSDI MNFSIYTSLS ITKSVLSLTE 650
    PARYMIMNSL AISSNVKDYI AEKFSPYTKT LFSVYMTRLI KNACFDAYDQ 700
    RQRVQLRDIY LSDYDITQKG IKDNRELTSI WFPGSVTLKE YLTQIYLPFY 750
    FNAKGLHEKH HVMVDLAKTI LEIECEQREN IKEIWSTNCT KQTVNLKILI 800
    HSLCKNLLAD TSRHNHLRNR IENRNNFRRS ITTISTFTSS KSCLKIGDFR 850
    KEKELQSVKQ KKILEVQSRK MRLANPMFVT DEQVCLEVGH CNYEMLRNAM 900
    PNYTDYISTK VFDRLYELLD KGVLTDKPVI EQIMDMMVDH KKFYFTFFNK 950
    GQKTSKDREI FVGEYEAKMC MYAVERIAKE RCKLNPDEMI SEPGDGKLKV 1000
    LEQKSEQEIR FLVETTRQKN REIDEAIEAL AAEGYESNLE KIEKLSLGKA 1050
    KGLKMEINAD MSKWSAQDVF YKYFWLIALD PILYPQEKER ILYFMCNYMD 1100
    KELILPDELL FNLLDQKVAY QNDIIATMTN QLNSNTVLIK RNWLQGNFNY 1150
    TSSYVHSCAM SVYKEILKEA ITLLDGSILV NSLVHSDDNQ TSITIVQDKM 1200
    ENDKIIDFAM KEFERACLTF GCQANMKKTY VTNCIKEFVS LFNLYGEPFS 1250
    IYGRFLLTSV GDCAYIGPYE DLASRISSAQ TAIKHGCPPS LAWVSIAISH 1300
    WMTSLTYNML PGQSNDPIDY FPAENRKDIP IELNGVLDAP LSMISTVGLE 1350
    SGNLYFLIKL LSKYTPVMQK RESVVNQIAE VKNWKVEDLT DNEIFRLKIL 1400
    RYLVLDAEMD PSDIMGETSD MRGRSILTPR KFTTAGSLRK LYSFSKYQDR 1450
    LSSPGGMVEL FTYLLEKPEL LVTKGEDMKD YMESVIFRYN SKRFKESLSI 1500
    QNPAQLFIEQ ILFSHKPIID FSGIRDKYIN LHDSRALEKE PDILGKVTFT 1550
    EAYRLLMRDL SSLELTNDDI QVIYSYIILN DPMMITIANT HILSIYGSPQ 1600
    RRMGMSCSTM PEFRNLKLIH HSPALVLRAY SKNNPDIQGA DPTEMARDLV 1650
    HLKEFVENTN LEEKMKVRIA INEAEKGQRD IVFELKEMTR FYQVCYEYVK 1700
    STEHKIKVFI LPTKSYTTTD FCSLMQGNLI KDKEWYTVHY LKQILSGGHK 1750
    AIMQHNATSE QNIAFECFKL ITHFADSFID SLSRSAFLQL IIDEFSYKDV 1800
    KVSKLYDIIK NGYNRTDFIP LLFRTGDLRQ ADLDKYDAMK SHERVTWNDW 1850
    QTSRHLDMGS INLTITGYNR SITIIGEDNK LTYAELCLTR KTPENITISG 1900
    RKLLGARHGL KFENMSKIQT YPGNYYITYR KKDRHQFVYQ IHSHESITRR 1950
    NEEHMAIRTR IYNEITPVCV VNVAEVDGDQ RILIRSLDYL NNDIFSLSRI 2000
    KVGLDEFATI KKAHFSKMVS FEGPPIKTGL LDLTELMKSQ DLLNLNYDNI 2050
    RNSNLISFSK LICCEGSDNI NDGLEFLSDD PMNFTEGEAI HSTPIFNIYY 2100
    SKRGERHMTY RNAIKLLIER ETKIFEEAFT FSENGFISPE NLGCLEAVVS 2150
    LIKLLKTNEW STVIDKCIHI CLIKNGMDHM YHSFDVPKCF MGNPITRDMN 2200
    WMMFREFINS LPGTDIPPWN VMTENFKKKC IALINSKLET QRDFSEFTKL 2250
    MKKEGGRSNI EFD 2263
    Length:2,263
    Mass (Da):263,013
    Last modified:October 1, 2002 - v1
    Checksum:iA612682C8495E627
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF528165 Genomic RNA. Translation: AAM94387.1.
    EF485035 Viral cRNA. Translation: ABQ12635.1.
    RefSeqiNP_671968.1. NC_004108.1.

    Genome annotation databases

    GeneIDi956554.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF528165 Genomic RNA. Translation: AAM94387.1 .
    EF485035 Viral cRNA. Translation: ABQ12635.1 .
    RefSeqi NP_671968.1. NC_004108.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956554.

    Family and domain databases

    InterProi IPR026382. CapSnatchArena.
    IPR029124. L_protein_N.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR014384. RNA-dir_pol_orthobunyavirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view ]
    Pfami PF04196. Bunya_RdRp. 1 hit.
    PF15518. L_protein_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000824. L_OrthobunV. 1 hit.
    TIGRFAMsi TIGR04202. capSnatchArena. 1 hit.
    PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain."
      Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J.
      Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes."
      Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S.
      Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiL_BUNL8
    AccessioniPrimary (citable) accession number: Q8JPR2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3