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Q8JPR1

- GP_BUNL8

UniProt

Q8JPR1 - GP_BUNL8

Protein

Envelope glycoprotein

Gene

GP

Organism
Bunyavirus La Crosse (isolate Human/United States/L78/1978)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.By similarity

    GO - Biological processi

    1. modulation by virus of host morphology or physiology Source: InterPro
    2. viral entry into host cell Source: UniProtKB-KW
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein
    Short name:
    GP
    Alternative name(s):
    M polyprotein
    Cleaved into the following 3 chains:
    Gene namesi
    Name:GP
    OrganismiBunyavirus La Crosse (isolate Human/United States/L78/1978)
    Taxonomic identifieri796210 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
    Virus hostiCervidae (deer) [TaxID: 9850]
    Homo sapiens (Human) [TaxID: 9606]
    Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
    Tamias [TaxID: 13712]
    ProteomesiUP000008768: Genome

    Subcellular locationi

    Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
    Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity.By similarity
    Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
    Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1066 – 10661W → A: Complete loss of viral fusion and entry. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1313Sequence AnalysisAdd
    BLAST
    Chaini14 – 14411428Envelope glycoproteinPRO_0000397205Add
    BLAST
    Chaini14 – 299286Glycoprotein G2By similarityPRO_0000397184Add
    BLAST
    Chaini300 – 473174Non-structural protein NSmBy similarityPRO_0000397185Add
    BLAST
    Chaini474 – 1441968Glycoprotein G1By similarityPRO_0000397186Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi490 – 4901N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1177 – 11771N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    G1 and G2 interacts with each other.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini14 – 200187LumenalSequence AnalysisAdd
    BLAST
    Topological domaini222 – 30584CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini327 – 36135LumenalSequence AnalysisAdd
    BLAST
    Topological domaini383 – 45068CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini472 – 1395924LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1417 – 143317CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei201 – 22121Sequence AnalysisAdd
    BLAST
    Transmembranei306 – 32621Sequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221Sequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121Sequence AnalysisAdd
    BLAST
    Transmembranei1396 – 141621Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1066 – 108722Fusion peptideSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR005167. Bunya_G1.
    IPR005168. Bunya_G2.
    IPR026400. Bunya_nonstruc_pro_NSm.
    IPR014413. M_poly_OrthobunV.
    [Graphical view]
    PfamiPF03557. Bunya_G1. 1 hit.
    PF03563. Bunya_G2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003944. M_poly_OrthobunV. 1 hit.
    TIGRFAMsiTIGR04210. bunya_NSm. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8JPR1-1 [UniParc]FASTAAdd to Basket

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    MICILVLITV AAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT     50
    EARYVRNATG VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL 100
    HTESYVCSAD CTIGVDKETA QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI 150
    TLDQTCEHLK VSCGPKSVQF HACFNQHMSC VRFLHRTILP GSIANSICQN 200
    IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYIY GIIYNKSCKK 250
    CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV 300
    MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EDLPDDMLEM 350
    ASRINSYYLT CILNYAVSWG LVIIGLLIGL LFKKYQHRFL NVYAMYCEEC 400
    DMYHDKSGLK RHGDFTNKCR QCTCGQYEDA AGLMAHRKTY NCLVQYKAKW 450
    MMNFLIIYIF LILIKDSAIV VQAAGTDFTT CLETESINWN CTGPFLNLGN 500
    CQKQQKKEPY TNIATQLKGL KAISVLDVPI ITGIPDDIAG ALRYIEEKED 550
    FHVQLTIEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN 600
    QHFCRCVKNG EKCSSSNWDF ANEMKDYYSG KQTKFDKDLN LALTALHHAF 650
    RGTSSAYIAT MLSKKSNDDL IAYTNKIKTK FPGNALLKAI IDYIAYMKSL 700
    PGMANFKYDE FWDELLYKPN PAKASNLARG KESSYNFKLA ISSKSIKTCK 750
    NVKDVACLSP RSGAIYASII ACGEPNGPSV YRKPSGGVFQ SSTDRSIYCL 800
    LDSHCLEEFE AIGQEELDAV KKSKCWEIEY PDVKLIQEGD GTKSCRMKDS 850
    GNCNVATNRW PVIQCENDKF YYSELQKDYD KAQDIGHYCL SPGCTTVRYP 900
    INPKHISNCN WQVSRSSIAK IDVHNIEDIE QYKKAITQKL QTSLSLFKYA 950
    KTKNLPHIKP IYKYITIEGT ETAEGIESAY IESEVPALAG TSIGFKINSK 1000
    EGKHLLDVIA YVKSASYSSV YTKLYSTGPT SGINTKHDEL CTGPCPANIN 1050
    HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC VFGSCQDIIK EELSVYRKET 1100
    EEVTDVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE TYSLPRIVAV 1150
    QNHEIKIGQI NDLGVYSKGC GNVQKVNGTI YGNGVPRFDY LCHLASRKEV 1200
    IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG 1250
    DVKYKTFADS VDITAEGSCT GCINCFENIH CELTLHTTIE ASCPIKSSCT 1300
    VFHDRILVTP NEHKYALKMV CTEKPGNTLT IKVCNTKVEA SMALVDAKPI 1350
    IELAPVDQTA YIREKDERCK TWMCRVRDEG LQVILEPFKN LFGSYIGIFY 1400
    TFIISIVVLL VIIYVLLPIC FKLRDTLRKH EDAYKREMKI R 1441
    Length:1,441
    Mass (Da):162,364
    Last modified:October 1, 2002 - v1
    Checksum:iD9A2BECC01821D1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF528166 Genomic RNA. Translation: AAM94388.1.
    EF485034 Viral cRNA. Translation: ABQ12634.1.
    RefSeqiNP_671969.1. NC_004109.1.

    Genome annotation databases

    GeneIDi956555.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF528166 Genomic RNA. Translation: AAM94388.1 .
    EF485034 Viral cRNA. Translation: ABQ12634.1 .
    RefSeqi NP_671969.1. NC_004109.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956555.

    Family and domain databases

    InterProi IPR005167. Bunya_G1.
    IPR005168. Bunya_G2.
    IPR026400. Bunya_nonstruc_pro_NSm.
    IPR014413. M_poly_OrthobunV.
    [Graphical view ]
    Pfami PF03557. Bunya_G1. 1 hit.
    PF03563. Bunya_G2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003944. M_poly_OrthobunV. 1 hit.
    TIGRFAMsi TIGR04210. bunya_NSm. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain."
      Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J.
      Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes."
      Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S.
      Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide."
      Plassmeyer M.L., Soldan S.S., Stachelek K.M., Roth S.M., Martin-Garcia J., Gonzalez-Scarano F.
      Virology 358:273-282(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TRP-1066.

    Entry informationi

    Entry nameiGP_BUNL8
    AccessioniPrimary (citable) accession number: Q8JPR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3