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Q8JPR1

- GP_BUNL8

UniProt

Q8JPR1 - GP_BUNL8

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Protein

Envelope glycoprotein

Gene

GP

Organism
Bunyavirus La Crosse (isolate Human/United States/L78/1978)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

GO - Biological processi

  1. modulation by virus of host morphology or physiology Source: InterPro
  2. viral entry into host cell Source: UniProtKB-KW
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:GP
OrganismiBunyavirus La Crosse (isolate Human/United States/L78/1978)
Taxonomic identifieri796210 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]
ProteomesiUP000008768: Genome

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs (By similarity).By similarity
Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini14 – 200187LumenalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121Sequence AnalysisAdd
BLAST
Topological domaini222 – 30584CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei306 – 32621Sequence AnalysisAdd
BLAST
Topological domaini327 – 36135LumenalSequence AnalysisAdd
BLAST
Transmembranei362 – 38221Sequence AnalysisAdd
BLAST
Topological domaini383 – 45068CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei451 – 47121Sequence AnalysisAdd
BLAST
Topological domaini472 – 1395924LumenalSequence AnalysisAdd
BLAST
Transmembranei1396 – 141621Sequence AnalysisAdd
BLAST
Topological domaini1417 – 143317CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell membrane Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1066 – 10661W → A: Complete loss of viral fusion and entry. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313Sequence AnalysisAdd
BLAST
Chaini14 – 14411428Envelope glycoproteinPRO_0000397205Add
BLAST
Chaini14 – 299286Glycoprotein G2By similarityPRO_0000397184Add
BLAST
Chaini300 – 473174Non-structural protein NSmBy similarityPRO_0000397185Add
BLAST
Chaini474 – 1441968Glycoprotein G1By similarityPRO_0000397186Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi490 – 4901N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1177 – 11771N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1066 – 108722Fusion peptideSequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view]
PIRSFiPIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsiTIGR04210. bunya_NSm. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JPR1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MICILVLITV AAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT
60 70 80 90 100
EARYVRNATG VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL
110 120 130 140 150
HTESYVCSAD CTIGVDKETA QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI
160 170 180 190 200
TLDQTCEHLK VSCGPKSVQF HACFNQHMSC VRFLHRTILP GSIANSICQN
210 220 230 240 250
IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYIY GIIYNKSCKK
260 270 280 290 300
CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV
310 320 330 340 350
MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EDLPDDMLEM
360 370 380 390 400
ASRINSYYLT CILNYAVSWG LVIIGLLIGL LFKKYQHRFL NVYAMYCEEC
410 420 430 440 450
DMYHDKSGLK RHGDFTNKCR QCTCGQYEDA AGLMAHRKTY NCLVQYKAKW
460 470 480 490 500
MMNFLIIYIF LILIKDSAIV VQAAGTDFTT CLETESINWN CTGPFLNLGN
510 520 530 540 550
CQKQQKKEPY TNIATQLKGL KAISVLDVPI ITGIPDDIAG ALRYIEEKED
560 570 580 590 600
FHVQLTIEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN
610 620 630 640 650
QHFCRCVKNG EKCSSSNWDF ANEMKDYYSG KQTKFDKDLN LALTALHHAF
660 670 680 690 700
RGTSSAYIAT MLSKKSNDDL IAYTNKIKTK FPGNALLKAI IDYIAYMKSL
710 720 730 740 750
PGMANFKYDE FWDELLYKPN PAKASNLARG KESSYNFKLA ISSKSIKTCK
760 770 780 790 800
NVKDVACLSP RSGAIYASII ACGEPNGPSV YRKPSGGVFQ SSTDRSIYCL
810 820 830 840 850
LDSHCLEEFE AIGQEELDAV KKSKCWEIEY PDVKLIQEGD GTKSCRMKDS
860 870 880 890 900
GNCNVATNRW PVIQCENDKF YYSELQKDYD KAQDIGHYCL SPGCTTVRYP
910 920 930 940 950
INPKHISNCN WQVSRSSIAK IDVHNIEDIE QYKKAITQKL QTSLSLFKYA
960 970 980 990 1000
KTKNLPHIKP IYKYITIEGT ETAEGIESAY IESEVPALAG TSIGFKINSK
1010 1020 1030 1040 1050
EGKHLLDVIA YVKSASYSSV YTKLYSTGPT SGINTKHDEL CTGPCPANIN
1060 1070 1080 1090 1100
HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC VFGSCQDIIK EELSVYRKET
1110 1120 1130 1140 1150
EEVTDVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE TYSLPRIVAV
1160 1170 1180 1190 1200
QNHEIKIGQI NDLGVYSKGC GNVQKVNGTI YGNGVPRFDY LCHLASRKEV
1210 1220 1230 1240 1250
IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG
1260 1270 1280 1290 1300
DVKYKTFADS VDITAEGSCT GCINCFENIH CELTLHTTIE ASCPIKSSCT
1310 1320 1330 1340 1350
VFHDRILVTP NEHKYALKMV CTEKPGNTLT IKVCNTKVEA SMALVDAKPI
1360 1370 1380 1390 1400
IELAPVDQTA YIREKDERCK TWMCRVRDEG LQVILEPFKN LFGSYIGIFY
1410 1420 1430 1440
TFIISIVVLL VIIYVLLPIC FKLRDTLRKH EDAYKREMKI R
Length:1,441
Mass (Da):162,364
Last modified:October 1, 2002 - v1
Checksum:iD9A2BECC01821D1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF528166 Genomic RNA. Translation: AAM94388.1.
EF485034 Viral cRNA. Translation: ABQ12634.1.
RefSeqiNP_671969.1. NC_004109.1.

Genome annotation databases

GeneIDi956555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF528166 Genomic RNA. Translation: AAM94388.1 .
EF485034 Viral cRNA. Translation: ABQ12634.1 .
RefSeqi NP_671969.1. NC_004109.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956555.

Family and domain databases

InterProi IPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view ]
Pfami PF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view ]
PIRSFi PIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsi TIGR04210. bunya_NSm. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain."
    Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes."
    Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S.
    Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide."
    Plassmeyer M.L., Soldan S.S., Stachelek K.M., Roth S.M., Martin-Garcia J., Gonzalez-Scarano F.
    Virology 358:273-282(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TRP-1066.

Entry informationi

Entry nameiGP_BUNL8
AccessioniPrimary (citable) accession number: Q8JPR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3