Q8JPR1 (GP_BUNL8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein
Short name=GP

Alternative name(s):
M polyprotein

Cleaved into the following 3 chains:

1. Glycoprotein G2
   Short name=Gn
2. Non-structural protein NSm
3. Glycoprotein G1
   Short name=Gc

Gene names

Name:

GP

Organism

Bunyavirus La Crosse (isolate Human/United States/L78/1978) [Reference proteome]

Taxonomic identifier

796210 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Orthobunyavirus › California encephalitis virus › Bunyavirus La Crosse

Virus host

Aedes triseriatus (Eastern treehole mosquito) (Ochlerotatus triseriatus) [TaxID: 7162]
Cervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Tamias [TaxID: 13712]

Protein attributes

Sequence length	1441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.
Subunit structure	G1 and G2 interacts with each other By similarity.
Subcellular location	Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity. Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region By similarity. Non-structural protein NSm: Host Golgi apparatus membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the orthobunyaviruses M polyprotein family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host Golgi apparatus Host endoplasmic reticulum Host membrane Membrane Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 13	13	Potential
	Chain	14 – 1441	1428	Envelope glycoprotein		PRO_0000397205
	Chain	14 – 299	286	Glycoprotein G2 By similarity		PRO_0000397184
	Chain	300 – 473	174	Non-structural protein NSm By similarity		PRO_0000397185
	Chain	474 – 1441	968	Glycoprotein G1 By similarity		PRO_0000397186
Regions
	Topological domain	14 – 200	187	Lumenal Potential
	Transmembrane	201 – 221	21	Potential
	Topological domain	222 – 305	84	Cytoplasmic Potential
	Transmembrane	306 – 326	21	Potential
	Topological domain	327 – 361	35	Lumenal Potential
	Transmembrane	362 – 382	21	Potential
	Topological domain	383 – 450	68	Cytoplasmic Potential
	Transmembrane	451 – 471	21	Potential
	Topological domain	472 – 1395	924	Lumenal Potential
	Transmembrane	1396 – 1416	21	Potential
	Topological domain	1417 – 1433	17	Cytoplasmic Potential
	Region	1066 – 1087	22	Fusion peptide Potential
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	490	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	1177	1	N-linked (GlcNAc...); by host Potential
Experimental info
	Mutagenesis	1066	1	W → A: Complete loss of viral fusion and entry. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8JPR1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D9A2BECC01821D1D	FASTA	1,441	162,364
	10 20 30 40 50 60 MICILVLITV AAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT EARYVRNATG 70 80 90 100 110 120 VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL HTESYVCSAD CTIGVDKETA 130 140 150 160 170 180 QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI TLDQTCEHLK VSCGPKSVQF HACFNQHMSC 190 200 210 220 230 240 VRFLHRTILP GSIANSICQN IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYIY 250 260 270 280 290 300 GIIYNKSCKK CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV 310 320 330 340 350 360 MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EDLPDDMLEM ASRINSYYLT 370 380 390 400 410 420 CILNYAVSWG LVIIGLLIGL LFKKYQHRFL NVYAMYCEEC DMYHDKSGLK RHGDFTNKCR 430 440 450 460 470 480 QCTCGQYEDA AGLMAHRKTY NCLVQYKAKW MMNFLIIYIF LILIKDSAIV VQAAGTDFTT 490 500 510 520 530 540 CLETESINWN CTGPFLNLGN CQKQQKKEPY TNIATQLKGL KAISVLDVPI ITGIPDDIAG 550 560 570 580 590 600 ALRYIEEKED FHVQLTIEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN 610 620 630 640 650 660 QHFCRCVKNG EKCSSSNWDF ANEMKDYYSG KQTKFDKDLN LALTALHHAF RGTSSAYIAT 670 680 690 700 710 720 MLSKKSNDDL IAYTNKIKTK FPGNALLKAI IDYIAYMKSL PGMANFKYDE FWDELLYKPN 730 740 750 760 770 780 PAKASNLARG KESSYNFKLA ISSKSIKTCK NVKDVACLSP RSGAIYASII ACGEPNGPSV 790 800 810 820 830 840 YRKPSGGVFQ SSTDRSIYCL LDSHCLEEFE AIGQEELDAV KKSKCWEIEY PDVKLIQEGD 850 860 870 880 890 900 GTKSCRMKDS GNCNVATNRW PVIQCENDKF YYSELQKDYD KAQDIGHYCL SPGCTTVRYP 910 920 930 940 950 960 INPKHISNCN WQVSRSSIAK IDVHNIEDIE QYKKAITQKL QTSLSLFKYA KTKNLPHIKP 970 980 990 1000 1010 1020 IYKYITIEGT ETAEGIESAY IESEVPALAG TSIGFKINSK EGKHLLDVIA YVKSASYSSV 1030 1040 1050 1060 1070 1080 YTKLYSTGPT SGINTKHDEL CTGPCPANIN HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC 1090 1100 1110 1120 1130 1140 VFGSCQDIIK EELSVYRKET EEVTDVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE 1150 1160 1170 1180 1190 1200 TYSLPRIVAV QNHEIKIGQI NDLGVYSKGC GNVQKVNGTI YGNGVPRFDY LCHLASRKEV 1210 1220 1230 1240 1250 1260 IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG DVKYKTFADS 1270 1280 1290 1300 1310 1320 VDITAEGSCT GCINCFENIH CELTLHTTIE ASCPIKSSCT VFHDRILVTP NEHKYALKMV 1330 1340 1350 1360 1370 1380 CTEKPGNTLT IKVCNTKVEA SMALVDAKPI IELAPVDQTA YIREKDERCK TWMCRVRDEG 1390 1400 1410 1420 1430 1440 LQVILEPFKN LFGSYIGIFY TFIISIVVLL VIIYVLLPIC FKLRDTLRKH EDAYKREMKI R

References

[1]	"Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain." Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes." Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S. Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide." Plassmeyer M.L., Soldan S.S., Stachelek K.M., Roth S.M., Martin-Garcia J., Gonzalez-Scarano F. Virology 358:273-282(2007) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF TRP-1066.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF528166 Genomic RNA. Translation: AAM94388.1. EF485034 Viral cRNA. Translation: ABQ12634.1.
RefSeq	NP_671969.1. NC_004109.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956555.
Family and domain databases
InterPro	IPR005167. Bunya_G1. IPR005168. Bunya_G2. IPR026400. Bunya_nonstruc_pro_NSm. IPR014413. M_poly_OrthobunV. [Graphical view]
Pfam	PF03557. Bunya_G1. 1 hit. PF03563. Bunya_G2. 1 hit. [Graphical view]
PIRSF	PIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMs	TIGR04210. bunya_NSm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GP_BUNL8

Accession

Primary (citable) accession number: Q8JPR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	October 1, 2002
Last modified:	January 9, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program