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Q8JPR1 (GP_BUNL8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Envelope glycoprotein

Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 3 chains:

  1. Glycoprotein G2
    Short name=Gn
  2. Non-structural protein NSm
  3. Glycoprotein G1
    Short name=Gc
Gene names
Name:GP
OrganismBunyavirus La Crosse (isolate Human/United States/L78/1978) [Reference proteome]
Taxonomic identifier796210 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]

Protein attributes

Sequence length1441 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.

Subunit structure

G1 and G2 interacts with each other By similarity.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity.

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region By similarity.

Non-structural protein NSm: Host Golgi apparatus membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the orthobunyaviruses M polyprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1313 Potential
Chain14 – 14411428Envelope glycoprotein
PRO_0000397205
Chain14 – 299286Glycoprotein G2 By similarity
PRO_0000397184
Chain300 – 473174Non-structural protein NSm By similarity
PRO_0000397185
Chain474 – 1441968Glycoprotein G1 By similarity
PRO_0000397186

Regions

Topological domain14 – 200187Lumenal Potential
Transmembrane201 – 22121 Potential
Topological domain222 – 30584Cytoplasmic Potential
Transmembrane306 – 32621 Potential
Topological domain327 – 36135Lumenal Potential
Transmembrane362 – 38221 Potential
Topological domain383 – 45068Cytoplasmic Potential
Transmembrane451 – 47121 Potential
Topological domain472 – 1395924Lumenal Potential
Transmembrane1396 – 141621 Potential
Topological domain1417 – 143317Cytoplasmic Potential
Region1066 – 108722Fusion peptide Potential

Amino acid modifications

Glycosylation571N-linked (GlcNAc...); by host Potential
Glycosylation4901N-linked (GlcNAc...); by host Potential
Glycosylation11771N-linked (GlcNAc...); by host Potential

Experimental info

Mutagenesis10661W → A: Complete loss of viral fusion and entry. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8JPR1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D9A2BECC01821D1D

FASTA1,441162,364
        10         20         30         40         50         60 
MICILVLITV AAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT EARYVRNATG 

        70         80         90        100        110        120 
VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL HTESYVCSAD CTIGVDKETA 

       130        140        150        160        170        180 
QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI TLDQTCEHLK VSCGPKSVQF HACFNQHMSC 

       190        200        210        220        230        240 
VRFLHRTILP GSIANSICQN IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYIY 

       250        260        270        280        290        300 
GIIYNKSCKK CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV 

       310        320        330        340        350        360 
MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EDLPDDMLEM ASRINSYYLT 

       370        380        390        400        410        420 
CILNYAVSWG LVIIGLLIGL LFKKYQHRFL NVYAMYCEEC DMYHDKSGLK RHGDFTNKCR 

       430        440        450        460        470        480 
QCTCGQYEDA AGLMAHRKTY NCLVQYKAKW MMNFLIIYIF LILIKDSAIV VQAAGTDFTT 

       490        500        510        520        530        540 
CLETESINWN CTGPFLNLGN CQKQQKKEPY TNIATQLKGL KAISVLDVPI ITGIPDDIAG 

       550        560        570        580        590        600 
ALRYIEEKED FHVQLTIEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN 

       610        620        630        640        650        660 
QHFCRCVKNG EKCSSSNWDF ANEMKDYYSG KQTKFDKDLN LALTALHHAF RGTSSAYIAT 

       670        680        690        700        710        720 
MLSKKSNDDL IAYTNKIKTK FPGNALLKAI IDYIAYMKSL PGMANFKYDE FWDELLYKPN 

       730        740        750        760        770        780 
PAKASNLARG KESSYNFKLA ISSKSIKTCK NVKDVACLSP RSGAIYASII ACGEPNGPSV 

       790        800        810        820        830        840 
YRKPSGGVFQ SSTDRSIYCL LDSHCLEEFE AIGQEELDAV KKSKCWEIEY PDVKLIQEGD 

       850        860        870        880        890        900 
GTKSCRMKDS GNCNVATNRW PVIQCENDKF YYSELQKDYD KAQDIGHYCL SPGCTTVRYP 

       910        920        930        940        950        960 
INPKHISNCN WQVSRSSIAK IDVHNIEDIE QYKKAITQKL QTSLSLFKYA KTKNLPHIKP 

       970        980        990       1000       1010       1020 
IYKYITIEGT ETAEGIESAY IESEVPALAG TSIGFKINSK EGKHLLDVIA YVKSASYSSV 

      1030       1040       1050       1060       1070       1080 
YTKLYSTGPT SGINTKHDEL CTGPCPANIN HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC 

      1090       1100       1110       1120       1130       1140 
VFGSCQDIIK EELSVYRKET EEVTDVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE 

      1150       1160       1170       1180       1190       1200 
TYSLPRIVAV QNHEIKIGQI NDLGVYSKGC GNVQKVNGTI YGNGVPRFDY LCHLASRKEV 

      1210       1220       1230       1240       1250       1260 
IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG DVKYKTFADS 

      1270       1280       1290       1300       1310       1320 
VDITAEGSCT GCINCFENIH CELTLHTTIE ASCPIKSSCT VFHDRILVTP NEHKYALKMV 

      1330       1340       1350       1360       1370       1380 
CTEKPGNTLT IKVCNTKVEA SMALVDAKPI IELAPVDQTA YIREKDERCK TWMCRVRDEG 

      1390       1400       1410       1420       1430       1440 
LQVILEPFKN LFGSYIGIFY TFIISIVVLL VIIYVLLPIC FKLRDTLRKH EDAYKREMKI 


R 

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References

[1]"Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain."
Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes."
Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S.
Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide."
Plassmeyer M.L., Soldan S.S., Stachelek K.M., Roth S.M., Martin-Garcia J., Gonzalez-Scarano F.
Virology 358:273-282(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF TRP-1066.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF528166 Genomic RNA. Translation: AAM94388.1.
EF485034 Viral cRNA. Translation: ABQ12634.1.
RefSeqNP_671969.1. NC_004109.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956555.

Family and domain databases

InterProIPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view]
PfamPF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view]
PIRSFPIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsTIGR04210. bunya_NSm. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGP_BUNL8
AccessionPrimary (citable) accession number: Q8JPR1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families