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Protein

Non-structural protein NS-S

Gene

N

Organism
Bunyavirus La Crosse (isolate Human/United States/L78/1978)
Status
Reviewed-Annotation score: Annotation score: 1 out of 5-Protein inferred from homologyi

Functioni

Inhibits host transcriptional machinery, by producing modifications to the phosphorylation state of the C-terminal domain (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II, suggesting that the elongation step of transcription and/or 3'-end processing is prevented. Inhibition of host transcription machinery leads to shut off of host cell protein synthesis and inhibition of the host innate immune response. NSs also seems to be involved in the nuclear relocalization of host PABP1 (By similarity).By similarity

GO - Biological processi

  1. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of eukaryotic host transcription initiation by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein NS-S
Gene namesi
Name:N
OrganismiBunyavirus La Crosse (isolate Human/United States/L78/1978)
Taxonomic identifieri796210 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]
ProteomesiUP000008768: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9292Non-structural protein NS-SPRO_0000397187Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiQ8JPQ9. 83 interactions.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 384Poly-Ser

Sequence similaritiesi

Family and domain databases

InterProiIPR000797. Bunya_NSs.
[Graphical view]
PfamiPF01104. Bunya_NS-S. 1 hit.
[Graphical view]
PIRSFiPIRSF003954. NS-S_OrthobunV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8JPQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSHQQVQMD LILMQGIWTS VLKMQNYSTL LQLGSSSSMP QRPRLLSRVS
60 70 80 90
QRGRLTLNLE SGRWRLSIII FLETGTTQLV TTILPSTDYL GI
Length:92
Mass (Da):10,467
Last modified:October 1, 2002 - v1
Checksum:i034D8B31E8C6D348
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF528167 Genomic RNA. Translation: AAM94390.1.
EF485033 Viral cRNA. Translation: ABQ12633.1.
RefSeqiNP_671971.1. NC_004110.1.

Genome annotation databases

GeneIDi956557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF528167 Genomic RNA. Translation: AAM94390.1.
EF485033 Viral cRNA. Translation: ABQ12633.1.
RefSeqiNP_671971.1. NC_004110.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8JPQ9. 83 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi956557.

Family and domain databases

InterProiIPR000797. Bunya_NSs.
[Graphical view]
PfamiPF01104. Bunya_NS-S. 1 hit.
[Graphical view]
PIRSFiPIRSF003954. NS-S_OrthobunV. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of the Bunyavirus, La Crosse virus, Human/78 strain."
    Hughes M.T., Kempf B.J., Blair C.D., Beaty B.J.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Genome sequence analysis of La Crosse virus and in vitro and in vivo phenotypes."
    Bennett R.S., Ton D.R., Hanson C.T., Murphy B.R., Whitehead S.S.
    Virol. J. 4:41-41(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "La Crosse bunyavirus nonstructural protein NSs serves to suppress the type I interferon system of mammalian hosts."
    Blakqori G., Delhaye S., Habjan M., Blair C.D., Sanchez-Vargas I., Olson K.E., Attarzadeh-Yazdi G., Fragkoudis R., Kohl A., Kalinke U., Weiss S., Michiels T., Staeheli P., Weber F.
    J. Virol. 81:4991-4999(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNSS_BUNL8
AccessioniPrimary (citable) accession number: Q8JPQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 1, 2002
Last modified: January 7, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.