Reviewed, UniProtKB/Swiss-Prot Q8JIL9 (DULRD_XENLA)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase dullard
    EC=3.1.3.16

Gene names

Name:

dullard

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine/threonine phosphatase which may be required for proper nuclear membrane morphology By similarity. Induces neuronal differentiation by antagonizing BMP signaling. Acts both by dephosphorylating BMPR1A and by promoting BMPR2 proteasomal degradation.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Interacts with bmpr1a, bmpr1b and bmpr2. Ref.3
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › perinuclear region.
Developmental stage	Expressed from egg to stage 35. Expression is restricted to the neural region as gastrulation proceeds, and is subsequently localized to neural tissues, branchial arches and pronephroi at the tail-bud stages. Ref.1
Sequence similarities	Belongs to the dullard family. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cytoplasm Membrane
Domain	Transmembrane
Molecular function	Developmental protein Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope organization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein serine/threonine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Serine/threonine-protein phosphatase dullard		PRO_0000297972
Regions
	Transmembrane	7 – 29	23	Potential
	Domain	58 – 225	168	FCP1 homology
Experimental info
	Mutagenesis	67	1	D → E: Strongly reduces phosphatase activity. Ref.3
	Mutagenesis	69	1	D → E: Strongly reduces phosphatase activity. Ref.3
	Sequence conflict	54	1	N → S in AAH82639. Ref.2
	Sequence conflict	150	1	G → A in AAH82639. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8JIL9-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 57E95520F55D26CC	FASTA	244	28,169
	10 20 30 40 50 60 MMRTPGLLGL RGFVAFAAKL WSFVLYLLRR QFRTIIQYQT VRYDVLPLSP ASRNRLSQVK 70 80 90 100 110 120 RKVLVLDLDE TLIHSHHDGV LRPTVRPGTP PDFILKVVID KHPVRFFVHK RPHVDFFLEV 130 140 150 160 170 180 VSQWYELVVF TASMEIYGSA VADKLDNNKG VLRRRFYRQH CTLELGSYIK DLSVVHSDLS 190 200 210 220 230 240 SVVILDNSPG AYRSHPDNAI PIKSWFSDPS DTALLNLLPM LDALRFTADV RSVLSRNLHQ HRLW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of dullard: a novel gene required for neural development." Satow R., Chan T.C., Asashima M. Biochem. Biophys. Res. Commun. 295:85-91(2002) [PubMed: 12083771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, FUNCTION. Tissue: Embryo.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"Dullard promotes degradation and dephosphorylation of BMP receptors and is required for neural induction." Satow R., Kurisaki A., Chan T.C., Hamazaki T.S., Asashima M. Dev. Cell 11:763-774(2006) [PubMed: 17141153] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-67 AND ASP-69, INTERACTION WITH BMPR1A; BMPR1B AND BMPR2, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB084264 mRNA. Translation: BAB92973.1. BC082639 mRNA. Translation: AAH82639.1.
RefSeq	NP_001084192.1. NP_001090256.1.
UniGene	Xl.5594 Xl.76057
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	399358. 779162.
KEGG	xla:399358. xla:779162.
Organism-specific databases
Xenbase	XB-FEAT-5939295. dullard.
Phylogenomic databases
HOVERGEN	Q8JIL9.
Enzyme and pathway databases
BRENDA	3.1.3.16. 648.
Family and domain databases
InterPro	IPR011948. Dullard. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02251. HIF-SF_euk. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DULRD_XENLA

Accession

Primary (citable) accession number: Q8JIL9
Secondary accession number(s): Q640I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project