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Protein

CTD nuclear envelope phosphatase 1

Gene

ctdnep1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein phosphatase that may dephosphorylate and activate lipins. Lipins are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at differents levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol (By similarity). Induces neuronal differentiation by antagonizing BMP signaling. Acts both by dephosphorylating BMPR1A and by promoting BMPR2 proteasomal degradation.By similarity2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. nervous system development Source: UniProtKB-KW
  3. nuclear envelope organization Source: UniProtKB
  4. positive regulation of triglyceride biosynthetic process Source: UniProtKB
  5. protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
CTD nuclear envelope phosphatase 1 (EC:3.1.3.16)
Alternative name(s):
Serine/threonine-protein phosphatase dullard
Gene namesi
Name:ctdnep1
Synonyms:dullard
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254032. ctdnep1.

Subcellular locationi

Membrane Curated; Single-pass membrane protein Curated. Cytoplasmperinuclear region 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2923HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. Nem1-Spo7 phosphatase complex Source: UniProtKB
  5. nuclear envelope Source: UniProtKB
  6. nuclear membrane Source: UniProtKB
  7. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671D → E: Strongly reduces phosphatase activity. 1 Publication
Mutagenesisi69 – 691D → E: Strongly reduces phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244CTD nuclear envelope phosphatase 1PRO_0000297972Add
BLAST

Expressioni

Developmental stagei

Expressed from egg to stage 35. Expression is restricted to the neural region as gastrulation proceeds, and is subsequently localized to neural tissues, branchial arches and pronephroi at the tail-bud stages.1 Publication

Interactioni

Subunit structurei

Interacts with bmpr1a, bmpr1b and bmpr2.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8JIL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 225168FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dullard family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG098153.
KOiK17617.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8JIL9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMRTPGLLGL RGFVAFAAKL WSFVLYLLRR QFRTIIQYQT VRYDVLPLSP
60 70 80 90 100
ASRNRLSQVK RKVLVLDLDE TLIHSHHDGV LRPTVRPGTP PDFILKVVID
110 120 130 140 150
KHPVRFFVHK RPHVDFFLEV VSQWYELVVF TASMEIYGSA VADKLDNNKG
160 170 180 190 200
VLRRRFYRQH CTLELGSYIK DLSVVHSDLS SVVILDNSPG AYRSHPDNAI
210 220 230 240
PIKSWFSDPS DTALLNLLPM LDALRFTADV RSVLSRNLHQ HRLW
Length:244
Mass (Da):28,169
Last modified:October 1, 2002 - v1
Checksum:i57E95520F55D26CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541N → S in AAH82639. 1 PublicationCurated
Sequence conflicti150 – 1501G → A in AAH82639. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB084264 mRNA. Translation: BAB92973.1.
BC082639 mRNA. Translation: AAH82639.1.
RefSeqiNP_001084192.1. NM_001090723.1.
NP_001090256.1. NM_001096787.1.
UniGeneiXl.5594.
Xl.76057.

Genome annotation databases

GeneIDi399358.
779162.
KEGGixla:399358.
xla:779162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB084264 mRNA. Translation: BAB92973.1.
BC082639 mRNA. Translation: AAH82639.1.
RefSeqiNP_001084192.1. NM_001090723.1.
NP_001090256.1. NM_001096787.1.
UniGeneiXl.5594.
Xl.76057.

3D structure databases

ProteinModelPortaliQ8JIL9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399358.
779162.
KEGGixla:399358.
xla:779162.

Organism-specific databases

CTDi399358.
779162.
XenbaseiXB-GENE-6254032. ctdnep1.

Phylogenomic databases

HOVERGENiHBG098153.
KOiK17617.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of dullard: a novel gene required for neural development."
    Satow R., Chan T.C., Asashima M.
    Biochem. Biophys. Res. Commun. 295:85-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, FUNCTION.
    Tissue: Embryo.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  3. "Dullard promotes degradation and dephosphorylation of BMP receptors and is required for neural induction."
    Satow R., Kurisaki A., Chan T.C., Hamazaki T.S., Asashima M.
    Dev. Cell 11:763-774(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-67 AND ASP-69, INTERACTION WITH BMPR1A; BMPR1B AND BMPR2, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCNEP1_XENLA
AccessioniPrimary (citable) accession number: Q8JIL9
Secondary accession number(s): Q640I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: October 1, 2002
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.