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Protein

Cysteine-rich venom protein triflin

Gene
N/A
Organism
Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Blocks contraction of smooth muscle elicited by high potassium-induced depolarization (PubMed:12047379). May target voltage-gated calcium channels (Cav) on smooth muscle.1 Publication

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Protein family/group databases

TCDBi8.B.9.1.1. the triflin toxin (triflin or crisp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich venom protein triflin
Short name:
CRVP
OrganismiProtobothrops flavoviridis (Habu) (Trimeresurus flavoviridis)
Taxonomic identifieri88087 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeProtobothrops

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 240221Cysteine-rich venom protein triflinPRO_0000006287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 153PROSITE-ProRule annotation1 Publication
Disulfide bondi92 ↔ 167PROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 164PROSITE-ProRule annotation1 Publication
Disulfide bondi186 ↔ 193PROSITE-ProRule annotation1 Publication
Disulfide bondi189 ↔ 198PROSITE-ProRule annotation1 Publication
Disulfide bondi202 ↔ 235PROSITE-ProRule annotation1 Publication
Disulfide bondi211 ↔ 229PROSITE-ProRule annotation1 Publication
Disulfide bondi220 ↔ 233PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Forms a stable, non-covalent complex with SSP-2.1 Publication

Structurei

Secondary structure

1
240
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 255Combined sources
Helixi31 – 4515Combined sources
Beta strandi52 – 543Combined sources
Helixi62 – 7211Combined sources
Helixi82 – 854Combined sources
Beta strandi93 – 1019Combined sources
Helixi105 – 1139Combined sources
Helixi114 – 1185Combined sources
Turni121 – 1233Combined sources
Beta strandi124 – 1274Combined sources
Helixi133 – 1386Combined sources
Beta strandi145 – 1528Combined sources
Beta strandi156 – 16813Combined sources
Turni173 – 1775Combined sources
Beta strandi182 – 1843Combined sources
Turni185 – 1884Combined sources
Beta strandi192 – 1943Combined sources
Helixi211 – 2177Combined sources
Helixi223 – 2286Combined sources
Helixi230 – 2345Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WVRX-ray2.40A20-240[»]
ProteinModelPortaliQ8JI39.
SMRiQ8JI39. Positions 20-240.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8JI39.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 166128SCPAdd
BLAST
Domaini202 – 23534ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004184.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
IPR002413. V5_allergen.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00838. V5ALLERGEN.
PR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JI39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAFIVLPIL AAVLQQSSGN VDFDSESPRK PEIQNEIIDL HNSLRRSVNP
60 70 80 90 100
TASNMLKMEW YPEAAANAER WAYRCIESHS SRDSRVIGGI KCGENIYMAT
110 120 130 140 150
YPAKWTDIIH AWHGEYKDFK YGVGAVPSDA VIGHYTQIVW YKSYRAGCAA
160 170 180 190 200
AYCPSSKYSY FYVCQYCPAG NIIGKTATPY KSGPPCGDCP SDCDNGLCTN
210 220 230 240
PCTRENEFTN CDSLVQKSSC QDNYMKSKCP ASCFCQNKII
Length:240
Mass (Da):26,752
Last modified:October 1, 2002 - v1
Checksum:i1EE3C9C426D974A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384219 mRNA. Translation: AAM45665.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384219 mRNA. Translation: AAM45665.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WVRX-ray2.40A20-240[»]
ProteinModelPortaliQ8JI39.
SMRiQ8JI39. Positions 20-240.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.9.1.1. the triflin toxin (triflin or crisp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004184.

Miscellaneous databases

EvolutionaryTraceiQ8JI39.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
IPR002413. V5_allergen.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00838. V5ALLERGEN.
PR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of novel snake venom proteins that block smooth muscle contraction."
    Yamazaki Y., Koike H., Sugiyama Y., Motoyoshi K., Wada T., Hishinuma S., Mita M., Morita T.
    Eur. J. Biochem. 269:2708-2715(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Venom and Venom gland.
  2. "Identification of novel serum proteins in a Japanese viper: homologs of mammalian PSP94."
    Aoki N., Sakiyama A., Deshimaru M., Terada S.
    Biochem. Biophys. Res. Commun. 359:330-334(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-49, SUBUNIT WITH SSP-2.
    Tissue: Venom.
  3. "Crystal structure of a CRISP family Ca2+ -channel blocker derived from snake venom."
    Shikamoto Y., Suto K., Yamazaki Y., Morita T., Mizuno H.
    J. Mol. Biol. 350:735-743(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 20-240, DISULFIDE BONDS.

Entry informationi

Entry nameiCRVP_PROFL
AccessioniPrimary (citable) accession number: Q8JI39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.