Reviewed, UniProtKB/Swiss-Prot Q8JH71 (ALDOB_DANRE)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase B
    EC=4.1.2.13
Alternative name(s):
    Liver-type aldolase

Gene names

Name:

aldob

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 364	363	Fructose-bisphosphate aldolase B		PRO_0000291611
Sites
	Active site	188	1	Proton acceptor By similarity
	Active site	230	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	364	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8JH71-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: FB2DD9CDDCF4DB9E	FASTA	364	39,288
	10 20 30 40 50 60 MTHQFPALST EQKKELATIA ERIVAPGKGI LAADESTGTM AKRFQKINVE NTEENRRSFR 70 80 90 100 110 120 DLLFSVDDSI SESIGGVILF HETLYQKSDK GVLFPKVIKD KGIVVGIKVD KGTAGLAGTD 130 140 150 160 170 180 GETTTQGLDG LSERCAQYKK DGCDFAKWRC VLKISDSCPS ALGIAENANV LARYASICQQ 190 200 210 220 230 240 NGLVPIVEPE ILPDGDHDLK QCQYATEKVL AAVYKALSDH HVYLEGTLLK PNMVTAGHSC 250 260 270 280 290 300 TKKYTPLEVA MATVTALRRT VPAAVPGICF LSGGQSEEEA SLNLNAMNQL SLHRPWKLSF 310 320 330 340 350 360 SYGRALQASA LSAWKGQAAN KKASQDAFVT RAKINSLASK GEYKPSGQAG QASTQSLFTA SYTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Negative charge correlates with neural expression in vertebrate aldolase isozymes." Merritt T.J.S., Quattro J.M. J. Mol. Evol. 55:674-683(2002) [PubMed: 12486526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Hematopoietic gene expression profile in zebrafish kidney marrow." Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y., Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X., Look A.T., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004) [PubMed: 15520368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney marrow.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF533646 mRNA. Translation: AAN04477.1. AY394965 mRNA. Translation: AAQ94592.1. BC062830 mRNA. Translation: AAH62830.1.
IPI	IPI00505184.
RefSeq	NP_919348.3.
UniGene	Dr.76309
3D structure databases
HSSP	HSSP built from PDB template 1QO5 based on UniProtKB P05062.
SMR	Q8JH71. Positions 3-364.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8JH71.
Genome annotation databases
Ensembl	ENSDART00000075744; ENSDARP00000070225; ENSDARG00000053684; Danio rerio. [Genome view]
GeneID	321664.
KEGG	dre:321664.
Organism-specific databases
CTD	321664.
ZFIN	ZDB-GENE-030131-383. aldob.
Phylogenomic databases
HOVERGEN	Q8JH71.
OMA	INQVPLH.
Enzyme and pathway databases
BRENDA	4.1.2.13. 96826.
Gene expression databases
ArrayExpress	Q8JH71.
Bgee	Q8JH71.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDOB_DANRE

Accession

Primary (citable) accession number: Q8JH71

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project