Reviewed, UniProtKB/Swiss-Prot Q8JH64 (BTK_CHICK)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase BTK
    EC=2.7.10.2
Alternative name(s):
    Bruton tyrosine kinase

Gene names

Name:

BTK

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a crucial role in B-cell ontogeny By similarity. UniProtKB Q06187
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity. UniProtKB Q06187
Enzyme regulation	Activated by phosphorylation By similarity.
Subunit structure	Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain By similarity.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Autophosphorylated on Tyr-220 and Tyr-549. The tyrosine phosphorylation of Tyr-220 may create a docking site for a SH2 containing protein By similarity. UniProtKB P35991
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. Contains 1 Btk-type zinc finger. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	SH2 domain SH3 domain Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Tyrosine-protein kinase BTK		PRO_0000088067
Regions
	Domain	3 – 132	130	PH
	Domain	214 – 270	57	SH3
	Domain	276 – 366	91	SH2
	Domain	400 – 653	254	Protein kinase
	Zinc finger	134 – 170	37	Btk-type
	Nucleotide binding	406 – 414	9	ATP By similarity UniProtKB P08631
Sites
	Active site	519	1	Proton acceptor By similarity UniProtKB P08631
	Metal binding	142	1	Zinc By similarity UniProtKB Q06187
	Metal binding	153	1	Zinc By similarity UniProtKB Q06187
	Metal binding	154	1	Zinc By similarity UniProtKB Q06187
	Metal binding	164	1	Zinc By similarity UniProtKB Q06187
	Binding site	428	1	ATP By similarity UniProtKB P08631
Amino acid modifications
	Modified residue	220	1	Phosphotyrosine; by autocatalysis By similarity UniProtKB P35991
	Modified residue	549	1	Phosphotyrosine; by autocatalysis By similarity UniProtKB P35991

Sequences

	Sequence		Length	Mass (Da)
	Q8JH64-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: FE5217E8E6195F6A	FASTA	657	75,879
	10 20 30 40 50 60 MASIILESIF LKRSQQKKKT SPLNFKKRLF LLTESKLSYY EYDFERGRRG SKKGSVDIEK 70 80 90 100 110 120 ITCVETVVPE NNPPPERQVP KKGEDYNMEQ ISIIERFPYP FQVVYDEGPL YVFSPTEELR 130 140 150 160 170 180 KRWIHQLKSV IRYNSDLVQK YHPCFWIDGQ YLCCSQTAKN AMGCKILESR NGSLKAGRSH 190 200 210 220 230 240 RKTKKPLPPT PEEDTMVMKP LPPEPAPSAA GEMKKVVALY NYVPMNVQDL QLQKGEDYLI 250 260 270 280 290 300 LEESHLPWWK ARDKNGREGY IPSNYVTATS NSLEIYEWYS KNITRSQAEQ LLKQEGKEGG 310 320 330 340 350 360 FIVRDSTSKT GKYTVSVYAK SAVDPQGMIR HYVVCCTPQN QYYLAEKHLF NTIPELITYH 370 380 390 400 410 420 QHNSAGLISR LKYPVSRHQK SAPSTAGLGY GSWEIDPKDL TFLKELGTGQ FGVVKYGKWR 430 440 450 460 470 480 GQYNVAIKMI REGSMSEDEF IDEAKVMMNL SHEKLVQLYG VCTKQRPIFI ITEYMANGCL 490 500 510 520 530 540 LNFLRETQRR FQPAELLEMC KDVCEAMEYL ESKQFLHRDL AARNCLVNDQ GIVKVSDFGL 550 560 570 580 590 600 SRYVLDDEYT SSMGSKFPVR WSPPEVLLYS KFSSKSDVWS FGVLMWEVYS LGKMPYERFN 610 620 630 640 650 NSETTEHVIQ GLRLYRPQQA SERVYAIMYS CWHEKAEERP TFSALLGSIV DITDEEP

References

[1]	"Btk expressed in primary chicken erythroblasts." Schmidt U., Haubenwallner S., Beug H., Nimpf J. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF535118 mRNA. Translation: AAN04043.2.
IPI	IPI00595247.
UniGene	Gga.1305
3D structure databases
SMR	Q8JH64. Positions 2-169, 128-201, 209-272, 267-385, 395-652.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8JH64.
Genome annotation databases
Ensembl	ENSGALT00000007950; ENSGALP00000007936; ENSGALG00000004958; Gallus gallus. [Genome view]
Phylogenomic databases
HOGENOM	HBG755340.
HOVERGEN	Q8JH64.
InParanoid	Q8JH64.
Enzyme and pathway databases
BRENDA	2.7.10.2. 4.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR001562. Znf_Btk_motif. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00779. BTK. 1 hit. PF00169. PH. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00402. TECBTKDOMAIN.
SMART	SM00107. BTK. 1 hit. SM00233. PH. 1 hit. SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. PS51113. ZF_BTK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BTK_CHICK

Accession

Primary (citable) accession number: Q8JH64

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)