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Q8JH47 (CDK8_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 8

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk8
Mediator of RNA polymerase II transcription subunit cdk8
Gene names
Name:cdk8
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the Mediator complex By similarity. Interacts with ccnc By similarity.

Subcellular location

Nucleus Probable.

Developmental stage

Expressed maternally. Expressed zygotically throughout development from the mid-blastula transition. Expression is concentrated in the head and trunk region of the embryo at the pharyngula stage of development. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 464464Cyclin-dependent kinase 8
PRO_0000312926

Regions

Domain21 – 335315Protein kinase
Nucleotide binding27 – 359ATP By similarity
Region1 – 1515Interaction with CCNC By similarity
Compositional bias373 – 3775Poly-Gln

Sites

Active site1511Proton acceptor By similarity
Binding site521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8JH47 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 291B2CF7EFD35A62

FASTA46453,234
        10         20         30         40         50         60 
MGYDFKVKLT GERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDRDYA LKQIEGTGIS 

        70         80         90        100        110        120 
MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDYA EHDLWHIIKF HRASKANKKP 

       130        140        150        160        170        180 
LQLPRGMVKS LLYQILDGIH YLHANWVLHR DLKPANILVM GEGPERGRVK IADMGFARLS 

       190        200        210        220        230        240 
NSPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 

       250        260        270        280        290        300 
IKTSNPYHHD QLDRIFNVMG FPADKDWEDI KKMPEHSTLM KDFRRNTYTN CSLIKYMEKH 

       310        320        330        340        350        360 
KVKPDSKAFH LLQKLLTMDP IRRITSEQAM QDPYFLEEPL PTSDVFAGCQ IPYPKREFLT 

       370        380        390        400        410        420 
EEEPEDKADK KNQQQQQGNN HTNGAGHTGN PDNSHAQGPP LKKVRVVPPT ATSSGLIMTS 

       430        440        450        460 
DYQRSNPHAA YQNPGPSTSL PQSSMGYSST SQQPPQYSHQ THRY 

« Hide

References

[1]"Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development."
Brabazon E.D., Bree R.T., Carton M.W., Grealy M., Byrnes L.
Biochim. Biophys. Acta 1576:203-208(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY007219 mRNA. Translation: AAG01892.1.
RefSeqNP_919389.1. NM_194408.1.
UniGeneDr.82150.

3D structure databases

ProteinModelPortalQ8JH47.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8JH47.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID373868.
KEGGdre:373868.

Organism-specific databases

CTD1024.
ZFINZDB-GENE-030903-2. cdk8.

Phylogenomic databases

eggNOGCOG0515.
HOVERGENHBG014652.
InParanoidQ8JH47.
KOK02208.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20813405.
PROQ8JH47.

Entry information

Entry nameCDK8_DANRE
AccessionPrimary (citable) accession number: Q8JH47
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families