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Q8JH47

- CDK8_DANRE

UniProt

Q8JH47 - CDK8_DANRE

Protein

Cyclin-dependent kinase 8

Gene

cdk8

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 359ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Cell division protein kinase 8
    Mediator complex subunit cdk8
    Mediator of RNA polymerase II transcription subunit cdk8
    Gene namesi
    Name:cdk8
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-030903-2. cdk8.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Cyclin-dependent kinase 8PRO_0000312926Add
    BLAST

    Proteomic databases

    PRIDEiQ8JH47.

    Expressioni

    Developmental stagei

    Expressed maternally. Expressed zygotically throughout development from the mid-blastula transition. Expression is concentrated in the head and trunk region of the embryo at the pharyngula stage of development.1 Publication

    Interactioni

    Subunit structurei

    Component of the Mediator complex. Interacts with ccnc.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8JH47.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 335315Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 1515Interaction with CCNCBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi373 – 3775Poly-Gln

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG014652.
    InParanoidiQ8JH47.
    KOiK02208.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8JH47-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGYDFKVKLT GERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDRDYA    50
    LKQIEGTGIS MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDYA 100
    EHDLWHIIKF HRASKANKKP LQLPRGMVKS LLYQILDGIH YLHANWVLHR 150
    DLKPANILVM GEGPERGRVK IADMGFARLS NSPLKPLADL DPVVVTFWYR 200
    APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD 250
    QLDRIFNVMG FPADKDWEDI KKMPEHSTLM KDFRRNTYTN CSLIKYMEKH 300
    KVKPDSKAFH LLQKLLTMDP IRRITSEQAM QDPYFLEEPL PTSDVFAGCQ 350
    IPYPKREFLT EEEPEDKADK KNQQQQQGNN HTNGAGHTGN PDNSHAQGPP 400
    LKKVRVVPPT ATSSGLIMTS DYQRSNPHAA YQNPGPSTSL PQSSMGYSST 450
    SQQPPQYSHQ THRY 464
    Length:464
    Mass (Da):53,234
    Last modified:October 1, 2002 - v1
    Checksum:i291B2CF7EFD35A62
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY007219 mRNA. Translation: AAG01892.1.
    RefSeqiNP_919389.1. NM_194408.1.
    UniGeneiDr.82150.

    Genome annotation databases

    GeneIDi373868.
    KEGGidre:373868.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY007219 mRNA. Translation: AAG01892.1 .
    RefSeqi NP_919389.1. NM_194408.1.
    UniGenei Dr.82150.

    3D structure databases

    ProteinModelPortali Q8JH47.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8JH47.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 373868.
    KEGGi dre:373868.

    Organism-specific databases

    CTDi 1024.
    ZFINi ZDB-GENE-030903-2. cdk8.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG014652.
    InParanoidi Q8JH47.
    KOi K02208.

    Miscellaneous databases

    NextBioi 20813405.
    PROi Q8JH47.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development."
      Brabazon E.D., Bree R.T., Carton M.W., Grealy M., Byrnes L.
      Biochim. Biophys. Acta 1576:203-208(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiCDK8_DANRE
    AccessioniPrimary (citable) accession number: Q8JH47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3